Search PubMed⌕ Search

Biomedical subjects

J Hein

Publications and source records attributed to J Hein.

At least 91 records · Page 5Linked to original sources

[Determination of O-agglutinins against Yersinia enterocolitica by the indirect hemagglutination test (author's transl)].

O-agglutinins against Yersinia enterocolitica serovar 03 and serovar 09 in serum specimens were determined by the indirect (passive) hemagglutination test and the Widal technique. The hemagglutination test was carried out in microtitre plates with formalized and sensitized turkey erythrocytes. Cell wall antigens (lipopolysaccharides) were prepared by the phenol-water method. The indirect hemagglutination test had a high sensitivity and specificity. Moreover, this test has the advantage of being easier to set up and read than the bacteria agglutination. No unspecific reactions were observed.

Agglutinins↗

[Immunoglobulins (IgG, secretory-IgA) and function of parotid gland in children with cystic fibrosis, with bronchial asthma, and with chronic bronchitis (author's transl)].

The immunoglobulins IgG and IgA (Secretory-IgA) were determined in connection with the parameters of secretion in parotid saliva. Children with cystic fibrosis, with bronchial asthma and with chronic bronchitis were compared. No significant differences were found for the groups of cystic fibrosis, of bronchial asthma, and of chronic bronchitis in comparison to a control group in relation to flow rate and protein content in parotid saliva. The comparison of the groups of diseases showed striking differences only with regard to the output of the gland after stimulation. The output of the gland per minute for IgG was decreased for patients with bronchial asthma as well as for patients with cystic fibrosis. No differences were found for the patients with chronic bronchitis and for the control group. Also the output of the gland for secretory-IgA was decreased after stimulation in the groups of disease: bronchial asthma and cystic fibrosis. The concentration of immunoglobulins in serum no correlation with the concentration of immunoglobulins in saliva neither for IgA nor for IgG. A high content of IgG in serum of patients with cystic fibrosis was observed. The mean values of IgA in serum of patients with chronic bronchitis were found to be significantly decreased.

Adolescent↗

[Septicaemia caused by Yersinia enterocolitica (author's transl)].

Yersinia enterocolitica (serotype 3 of Winblad, or O-group 1 of Knapp) was isolated from two blood cultures of a 52-year-old patient with chronic hepatitis, pancytopenia and paroxysmal nocturnal haemoglobinuria who had been hospitalised because of septic temperatures and chills. The serum-agglutinin titre was 1:10.000 both against the isolated strain and a reference strain (MyO; serotype 3). The titre had decreased to 1:2.500 and 1:640, respectively, in serum samples taken ten and 40 days later. The causative organism was not isolated from stool specimens, but the patient had been treated with antibiotics. Strains identical biochemically, serologically and by lysotypi-g were found in the stool specimens of the patient's wife and dog. The agglutinin titres were 1:320 in the wife and 1:20 in the dog. A son, who was living in the same house, had a negative stool culture and serology.

Anemia, Aplastic↗