Search PubMed⌕ Search

Biomedical subjects

J Hau

Publications and source records attributed to J Hau.

At least 91 records · Page 5Linked to original sources
PubMed1985

The effect on pregnancy of intrauterine administration of antibodies against two pregnancy-associated murine proteins: murine pregnancy-specific beta 1-glycoprotein and murine pregnancy-associated alpha 2-glycoprotein.

Intrauterine administration of 100 arbitrary units (AU) of purified monospecific rabbit anti murine pregnancy specific beta 1-glycoprotein antibodies resulted in the loss of the fetuses in pregnant mice (14 out of 14). By contrast, a similar treatment with 100 AU of rabbit anti murine pregnancy-associated alpha 2-glycoprotein had an effect similar to saline on the outcome of pregnancy. Intravenous administration of 1000 AU of rabbit anti murine pregnancy specific beta 1-glycoprotein during pregnancy in mice had no effect on the outcome of pregnancy.

Animals↗
PubMed1985

Placental function studies in low birth weight infants with and without dysmaturity.

Maternal blood levels of human placental lactogen and schwangerschaftsprotein 1 were measured in 51 women who delivered a growth-retarded infant. The levels were substantially lower in the 27 women whose infants were clinically dysmature than in the 24 women whose infants were small but of normal appearance. About one-half (44%) the cases of true dysmaturity had abnormal concentrations of human placental lactogen (less than 4 mg/L), whereas none of the small but normal group had values in this zone. It is concluded that biochemical tests of this type reflect dynamic aspects of placental function and not simply the overall size of the fetus and placenta.

Female↗
PubMed1984

The third complement factor (C3) and its in vivo cleavage products: interaction with lectins and precipitation with polyethylene glycol.

Five molecular forms of C3 expressing D but not C epitopes were identified following in vivo activation of the complement system. Examination of concanavalin A (Con-A) reactivity in crossed immunoelectrophoresis revealed that native C3, C3c and the beta mobile form 4 of C3d were completely precipitated by 100 micrograms Con A/cm2. The alpha-1 mobile form 1 of C3d did not interact with Con A, whereas the alpha-2 mobile forms 2 and 3 were retarded in electrophoretic migration by Con A. Native C3, C3c, and forms 4 and 5 of C3d were precipitated by 12% (w/v) polyethylene glycol (PEG). Form 1 of C3d was soluble in these PEG concentrations, whereas forms 2 and 3 were partially precipitated.

Antigen-Antibody Reactions↗
PubMed1984

Placental protein measurements in complicated pregnancies. I. Intrauterine growth retardation.

Maternal serum levels of human placental lactogen (hPL), schwangerschaftsprotein 1 (SP1) and pregnancy-associated plasma protein A (PAPP-A) were measured serially throughout pregnancy in 753 women who had a normal pregnancy when recruited during the second trimester. Thirty-three women were delivered of an infant with low birth-weight and with phenotypic features of intrauterine growth retardation (IUGR). The predictive value of an abnormal (less than 10th centile) hPL result (PVpos) in the identification of IUGR was between 28 and 32%, the sensitivity (36-54%) being greatest at 35 weeks gestation. The predictive value of a normal result (PVneg) was 87-96% at various stages of pregnancy, also greatest at 35 weeks gestation. For SP1, the sensitivity and predictive values were also greatest at 35 weeks gestation (PVpos, 20%; sensitivity, 32%; PVneg, 95%), but for PAPP-A these values were considerably less at all gestations. The trends in levels of hPL, SP1 and PAPP-A observed in individual patients with IUGR were not apparently related to any clinically recognizable feature of the pregnancy or the degree of fetal compromise, irrespective of whether the levels were within or outside the 80% confidence limits of the normal range or whether the levels fell from within the normal range. These data suggest that maternal hPL measurements are superior in the identification of IUGR in samples obtained at 30-35 weeks gestation.

Female↗
PubMed1984

Placental protein measurements in complicated pregnancies. II. Pregnancy-related hypertension.

Maternal serum levels of pregnancy-associated plasma protein A (PAPP-A), human placental lactogen (hPL) and schwangerschafts-protein 1 (SP1) were measured serially during the second and third trimesters in 753 women with a normal pregnancy when recruited during the second trimester. Thirty-seven pregnancies were complicated by pregnancy-related hypertension after 28 weeks gestation. Maternal levels of PAPP-A and SP1, and trends of levels in individual patients, could generally not be distinguished from those seen in patients with a normal pregnancy, and were unrelated to the time of onset of the disease, its severity or the occurrence of other complications with one exception, in which decreased levels of SP1 and hPL were seen. Mean levels of hPL were significantly lower (P less than 0.05) at 35 weeks gestation. These data suggest that the measurement of the placental proteins examined here is of no value in the prediction of occurrence of pregnancy-related hypertension.

Female↗
PubMed1984

Placental protein measurements in complicated pregnancies. III. Premature labour.

Maternal serum levels of pregnancy-associated plasma protein. A (PAPP-A), human placental lactogen (hPL) and schwangerschafts-protein 1 (SP1) were measured serially during second and third trimester in 753 women with normal pregnancy when they were recruited to the study. In 24 women spontaneous premature labour occurred before 37 completed weeks and these women had significantly lower mean levels of serum SP1 at 29-31 weeks and at 33-34 weeks gestations but similar mean levels of serum PAPP-A and hPL at all gestations compared with corresponding values in normal pregnancy. The predictive value of an abnormal SP1 result was 5.2% at 29-31 weeks and 10.3% at 33-34 weeks. Furthermore, trends of levels of the three placental proteins in individual patients were similar to those seen in normal pregnancy, and the trends were unrelated to the occurrence of other complications and the time of onset of labour. This study suggests that measurements of the three placental proteins are unlikely to be of any value in the prediction of spontaneous premature labour.

Female↗
PubMed1984

Examination of Aleutian disease virus in charge-shift crossed immunoelectrophoresis.

The surface properties of Aleutian disease virus were studied by charge-shift crossed immunoelectrophoresis. When different strains of Aleutian disease virus were treated with non-charged detergent followed by charged detergents, they showed bi-directional migration velocity shifts in electrophoresis, indicating amphiphilic surface properties of the virus.

Aleutian Mink Disease Virus↗
PubMed1984

Charge and size heterogeneity of C3d following in vivo and in vitro activation of the complement system.

Split products of the third complement factor (C3) expressing D but not C epitopes (C3d) were analyzed by crossed immunoelectrophoresis and size chromatography. Four molecular forms (termed 1, 2, 3, and 4 from the anodic side) were identified. The precipitation pattern of C3d in serum following acute in vivo activation was similar to the patterns observed using in vitro activation by MgCl2, zymosan, Escherichia coli, and delta IgG. These patterns were different from those observed in normal human serum and serum from a patient suffering from systemic lupus erythematosus. The molecular weights of forms 1 and 4 were approximately 170,000 daltons and those of forms 2 and 3 approximately 40,000 daltons.

Complement Activation↗
PubMed1984

Characterization of the analogues to human pregnancy-associated alpha 2-glycoprotein (alpha 2PAG, PZP) isolated in the mouse and rat.

Immunological cross-reaction and antigenic identity between pregnancy-associated alpha 2-glycoprotein (alpha 2PAG) and alpha 2PAG analogues in the mouse and the rat are demonstrated. The proteins have been characterized independently in 2 different laboratories and the physicochemical and biological properties of the rat and the mouse alpha 2PAG analogues and human alpha 2PAG are compared and discussed.

Animals↗
PubMed1984

Induction of murine alpha-foetoprotein synthesis by oestradiol.

The synthesis of murine alpha-foetoprotein (m-AFP) was induced in 43 out of 47 adult mice of both sexes by sc administration of 100 micrograms oestradiol-17 beta every second day. The m-AFP serum levels of the oestrogen treated male and female mice were 7 and 17%, respectively, of the levels in mice during late pregnancy. Immunohistochemical examination of liver tissue revealed the intracellular presence of m-AFP in less than 0.5% of the hepatocytes scattered throughout the liver of the oestrogen treated mice.

Animals↗
PubMed1983

Quantification of corticosteroid binding globulin by electroimmunoassay during human pregnancy.

Corticosteroid binding globulin (CBG) was purified by one step positive immunospecific affinity chromatography and antibody to human CBG produced in goats. The goat anti-human CBG was used in the development of a precise rocket immunoelectrophoresis for CBG quantification. CBG levels measured by this assay were found to increase from 30 AU/ml to 100 AU/ml during pregnancy. There was no statistically significant association observed between levels of CBG, or circulating estriol, or pregnancy zone protein. Circulating CBG was estimated in 32 women with pregnancy associated hypertension, the levels were all within the normal range, and not significantly different from those seen in matched controls.

Animals↗
PubMed1983

The influence of pituitary and gonadal hormones on serum levels of pregnancy-associated murine protein-1.

Hypophysectomy of female mice resulted in the disappearance of pregnancy-associated murine protein-1 (PAMP-1) from the circulation within a week. Maintenance of physiological levels of oestrogen, progesterone, testosterone, LH, FSH or prolactin was not sufficient to maintain a normal PAMP-1 level in the hypophysectomized animals. However, administration of oestrogen in large doses to adult male mice with undectatable levels of circulating PAMP-1 caused PAMP-1 to appear in the blood. Testosterone treatment of females inhibited the PAMP-1 synthesis.

Animals↗
PubMed1983

Immunochemical demonstration of a new pregnancy protein in the mare.

An antiserum against the serum of a pregnant mare was absorbed with stallion serum. This antiserum then gave two precipitates in crossed immunoelectrophoresis with serum from pregnant mares as the antigen. The two precipitates exhibited beta-1 and alpha-2 electrophoretic mobility. Identity was demonstrated between the alpha-2 mobile protein and PMSG. The absorbed antiserum inhibited the biological action of the PMSG preparation when tested in mouse ovarian weight assays. The beta-1 mobile protein was not detected in the serum from non-pregnant mares, stallions or geldings and was detected earlier in pregnancy (Day 30) than was PMSG (Day 42).

Animals↗
PubMed1983

Heterogeneity in electrophoretic mobility of C3-derived molecules expressing D but not C-epitopes following in vivo activation of the complement system.

Four different populations of C3-derived molecules expressing D but not C epitopes were identified following in vivo activation of the complement system. The four molecular forms, differing in electrophoretic migration velocity, were assigned the nos. 1, 2, 3 and 4 after decreasing electrophoretic mobility. Analysis of the time-dependent changes in the relative concentration of the different molecular forms demonstrated an increase in the plasma concentration of population 4 and a decrease of populations 2 and 3, whereas form 1 remained rather constant after acute activation of the complement system.

Animals↗
PubMed1982

Affinity electrophoresis of glycoproteins.

Affinity electrophoresis is based on the reaction between interacting components during electro phoresis. In this review is given the general analytical technology. The main advantages of the analytical electrophoresis approach appear to be: 1. It can separate macromolecules that interact with a specific ligand from those that do not. 2. It can be used for studies of interacting macromolecules. 3. It is not necessary to purify interacting components. 4. A multitude of proteins reacting with the same ligand may be studied simultaneously. 5. It can be generalized to interactions other than those between lectins and glycoproteins.

Animals↗
PubMed1982

Circulating C3, C4, and C3 split products (C3c and C3d) during normal pregnancy.

The plasma concentrations of the complement components C3 and C4, as well as the split products C3c and C3d, were measured before, during, and after normal pregnancy. Significantly increased values were observed in the C3 and C3d levels in the second and third trimesters of pregnancy. The level of C4 was not significantly affected by pregnancy and C3c could not be detected using electroimmunoassays. These results suggest that the increased C3 split-product levels observed reflected an increased turnover of native C3 rather than activation of the complement cascade.

Complement C3↗