Erroneous densitometric albumin estimation as a result of prestaining with certain dyes.
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Biomedical subjects
Publications and source records attributed to J Hattingh.
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The effectiveness of EDTA (dipotassium and disodium salts) and heparin as anticoagulants for the blood of puffadders and pigeons has been investigated. In the case of the snakes all three substances produced haemolysis after variable time intervals, and increases in cell volume were observed. In the case of the pigeons, samples stored at 4 degrees C showed lower haematocrit values and haemolysed sooner than samples stored at room temperature.
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Comparative polyacrylamide electrophoretic studies were done on the cerebrospinal fluid (CSF) of rabbits, pigeons, frogs and snakes. The results are compared to the pattern of normal human CSF and it is suggested that significant differences between the human and lower animals might exist.
The possible use of the swimbladder of carp as auxiliary respiratory organ when the animals are exposed to air has been studied. After exposure to air, blood pH and pO2 and mean swimbladder O2 concentration decrease and blood pCO2 increases in normal animals. Exposure to air after ligation of the pneumatic ducts results in increases in blood pH and pCO2 and decreases in blood pO2 and swimbladder carbon dioxide concentration. Possible reasons for these observations are discussed.
This study compares the effects of ethylenediamine tetra-acetate (EDTA) and heparin in various concentrations as anticoagulants on the microhaematocrit values of the blood of five species of freshwater fish. EDTA always produced haemolysis after a time interval ranging from 1 to 48 hrs and increased cell volume. Heparin in powdered form was found to be the anticoagulant of choice although minor haematological changes do occur, but it is stressed that preliminary experiments must be done to examine the effects of the anticoagulant before routine haematologic analyses are preformed.
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