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Biomedical subjects

J Hata

Publications and source records attributed to J Hata.

At least 235 records · Page 13Linked to original sources
PubMed1986

Serotonin in tubular adenomas, adenocarcinomas and endocrine tumours of the stomach. An immunohistochemical study.

Serotonin was examined immunohistochemically in seven tubular adenomas, 194 adenocarcinomas and 41 endocrine cell tumours of the stomach. In tubular adenomas, serotonin-containing cells showing argentaffinity were present in the lower portion of the adenomatous glands and were considered to be an expression of intestinal character. Scattered serotonin-containing tumour cells were found in 60 (30.9%) of 194 adenocarcinomas regardless of their histological type. Cell fusions between carcinoma and enterochromaffin (EC) cells might be a possible mechanism for the occurrence of serotonin-containing cells within the tumour. In 17 (54.8%) of 31 endocrine cell carcinomas, serotonin-containing tumour cells were observed in a variable degree in contrast to the absence of these cells in classical carcinoid. Moreover, diffuse serotonin reactivity was found in four cases of scirrhous endocrine cell carcinoma. The histogenesis and the occurrence of serotonin-containing cells in each type of gastric tumour is also discussed.

Adenocarcinoma↗
PubMed1986

Biochemical and morphological studies of human diploid and fluoride-resistant fibroblasts in vitro.

Fluoride-resistant (FR 30) lines were produced from human diploid fibroblast (NDU-1) cells by progressively increasing the concentration of fluoride (F) in the growth medium up to 1.58 mM. This concentration completely inhibited the growth of the original cells. The resistant cells had decreased incorporation of [14C]-leucine and an increased rate of the cell division. The activities of acid phosphatase, alkaline phosphatase, lactate dehydrogenase, leucine aminopeptidase, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and alpha-hydroxybutyrate dehydrogenase in the FR 30 cells were lower than in the NDU-1 cells. The FR 30 cells had irregular shapes and sizes; the amount of undeveloped rough endoplasmic reticulum and the number of lysosomes were increased. These biochemical and morphological changes in FR 30 cells suggest that their metabolic activities were depressed by F, although they have some degree of F resistance.

Cell Division↗
PubMed1986

Human epidermal growth factor in gastric carcinoma as a biologic marker of high malignancy.

The presence of human epidermal growth factor (hEGF) was studied in a total of 210 gastric carcinomas comprising 52 early carcinomas, 113 advanced carcinomas and 45 scirrhous carcinomas. An immunohistochemical study revealed no hEGF-immunoreactivity in early gastric carcinomas, while hEGF-positive tumor cells were detected in 24 (21.2%) of the 113 advanced carcinomas and in 15 (33.3%) of the 45 scirrhous carcinomas. The incidence of hEGF-immunoreactivity in well-differentiated adenocarcinomas was significantly higher than that in poorly differentiated adenocarcinomas (P less than 0.05). Moreover, hEGF-immunoreactive tumor cells were observed in 13 (30.4%) of the 42 scirrhous poorly differentiated adenocarcinomas, the incidence being significantly higher than that in non-scirrhous poorly differentiated adenocarcinomas (P less than 0.05). The average hEGF content in the tumor tissue estimated by radioimmunoassay was 3.77 +/- 0.61 (mean +/- SE) ng/g wet weight in immunohistochemical hEGF-positive tumors and 2.19 +/- 0.18 ng/g wet weight in hEGF-negative tumors, the difference being significant (P less than 0.05). Patients with hEGF-positive carcinomas (excluding scirrhous carcinomas) had much worse prognosis than those with hEGF-negative carcinomas. These results suggest that EGF produced by tumor cells plays an important role in the invasive growth and productive fibrosis of gastric carcinoma and also serves as a biologic marker of high malignancy in patients with gastric cancers.

Adenocarcinoma↗
PubMed1986

Monoclonal antibodies with fine specificities distinguishing alpha-fetoproteins of hepatoma and yolk sac tumor origin.

Monoclonal antibodies with fine specificities distinguishing alpha-fetoproteins of hepatoma (HEP-AFP) and yolk sac tumor (YST-AFP) origin have been obtained. These murine antibodies were produced by hybridomas made by fusion of X63-Ag8.653 myeloma cells with BALB/c spleen cells immunized with either HEP-AFP or YST-AFP and selected for their differential association with these antigens on the basis of Scatchard plot analysis. Three monoclonals (MA120, MA132 and MA136) selectively reacted with HEP-AFP. Their reactivity with YST-AFP was low. One monoclonal (MA122) reacted strongly with YST-AFP, whereas the reaction with HEP-AFP was significantly less strong. The difference in the association constants of these antibodies for the two AFPs appeared to be due to their specificity for the carbohydrate portions of the AFPs, which are different, at least in part. Indirect immunoperoxidase staining confirmed that MA122 was able to stain sections of an infantile embryonal carcinoma, but not of hepatoma.

Animals↗
PubMed1986

Murine cytomegalovirus infection model in Balb/c mice--1. Virological and pathological profiles in mice inoculated with various virus doses.

By intraperitoneal injection of murine cytomegalovirus (MCMV) in Balb/c mice at doses of 2 X 10(6), 2 X 10(5) and 2 X 10(4) pfu per mouse, lethal, acute but non-lethal and asymptomatic infection were produced respectively. 100% mortality was found in mice infected with the lethal dose, as well as high MCMV titres in liver and spleen, severe necrosis of the spleen and severe degeneration of the liver. In these mice leukocyte counts in blood was not raised and inflammatory responses in the peritoneum, spleen and liver were not observed. Mice with acute but non-lethal infection showed less severe pathological changes, moderate spleen necrosis was followed by spleen enlargement. In this group of mice, severe mononucleosis was observed, and moderate inflammatory reactions were noted in the peritoneum, liver and spleen. Mice asymptomatically infected exhibited slight mononucleosis in the blood and splenomegaly.

Animals↗
PubMed1986

Murine cytomegalovirus infection model in Balb/c mice--2. Pericarditis with myocardial involvement during virus infection.

Pericarditis with myocardial involvement was observed in mice infected with doses of murine cytomegalovirus (MCMV) designed to produce lethal, acute but non-lethal and asymptomatic infections. White spot was noted on the surface of the right ventricle from 4 to 6 days after MCMV infection. MCMV was isolated from the heart tissues as early as 2 days after infection. The virus titre reached a peak on day 6 and thereafter rapidly declined to undetectable level. Histopathological changes appeared on day 2 to 4 when necrosis of myocardial fibres occurred in the subpericardial region. This was followed by acute inflammatory cellular infiltration on day 6. After that organization of fibrin took place and fibrotic lesion and cellular infiltration could still be observed after 18 days. The pathological changes could only be seen in the right ventricle. Peroxidase-labelled antibody technique was carried out to detect MCMV antigen in the cardiac tissue. MCMV antigen was found in the pericardium only at the early stage of infection.

Animals↗
PubMed1986

Teratocarcinoma derived from mouse zygotes after the introduction of activated human c-Ha-ras DNA.

We attempted to produce transgenic mice harboring the normal or activated human c-Ha-ras gene in order to examine the function of activated oncogenes in tumorigenesis. During the process of development, it happened that not only normal looking fetuses were obtained but also malformants, developmentally arrested conceptuses and tumors. Six such abnormally developed embryos were found to have been integrated with the activated human c-Ha-ras gene. One of the two tumors thus obtained was formed after the injection of 6.3 kb DNA fragment containing the gene for p21 with valine at the twelfth position. This tumor was integrated with two copies of the introduced DNA at a particular site in a chromosome and arranged in tandem in a head to tail direction. Histological analysis revealed that this tumor was constructed from at least three types of cells: two originating from different germ layers (one endoderm and the other mesoderm) and the third from an extra-embryonic ectoderm. The other tumor revealed similar features. Thus, it was strongly suggested that these tumors were derived from the very early developmental stage of the embryo, and had features very similar to those of teratocarcinoma.

Animals↗
PubMed1986

Infantile digital fibromatosis. An electron microscopic and immunohistochemical study.

Three cases of infantile digital fibromatosis were studied by electron microscopy and immunohistochemistry. The tumor was made up equally of myofibroblasts containing long narrow bundles of microfilaments with dense bodies. Another striking feature was the intracytoplasmic electron-dense inclusion. The inclusions were constituted of packed fibrils similar to those of the long bundles. At the periphery of these inclusions were seen prominent felt-like filamentous structures; the diameter varied from 5-12 nm. The 10 nm filaments were seen most abundantly in the cytoplasm surrounding the inclusions, but sometimes they were also found within the inclusions themselves, mainly at their periphery. These findings suggest that the inclusions may represent an abnormal accumulation of cytoskeletal proteins. On the other hand, various kinds of immunohistochemical study showed that actin, actomyosin, myosin, and vimentin were distinctly observed in cytoplasms of tumor cells. They were located fringing the intracytoplasmic inclusions, but the inclusion themselves showed negative findings, thus indicating a hollow-like staining pattern. These data can be synthesized as follows. The inclusion results from an accumulation of cytoskeletal proteins (actin-myosin complex and vimentin, at least), and antigenicity seems to be lost by for a certain mechanism or in the degenerative process.

Child, Preschool↗
PubMed1985

A malignant cystosarcoma phyllodes with positive estrogen receptor and its heterotransplantation into nude mice.

We detected cytosol estrogen receptors in a malignant cystosarcoma phyllodes and succeeded in serial transplantations into athymic nude mice. The tumors of this transplantable strain (MC-3-JCK) have the same histologic features as the original tumors, and retain significant amounts of cytosol estrogen receptors. This strain appears to provide a useful experimental model for the study of biologic and therapeutic aspects of human cystosarcoma phyllodes of the breast.

Adult↗
PubMed1985

Congenital giant craniopharyngioma.

A case of a huge congenital craniopharyngioma in a male infant was reported with a review of five similar cases from the literature.

Craniopharyngioma↗
PubMed1985

'So-called mesothelioma' of the atrioventricular node--immunohistochemical study.

So-called mesothelioma of the atrioventricular node is a rare lesion and a cause of sudden, unexpected death. A case of congenital complete heart block accompanied by polyclonal immunoblastosis is presented. The histological features of our case are fundamentally similar to that of the cases previously reported. Immunohistochemical findings indicated that the tumor may have originated from aberrant epithelial or mesothelial tissue.

Adult↗
PubMed1984

Malignant granular cell tumor of the right sciatic nerve. Report of an autopsy case with electron microscopic, immunohistochemical, and enzyme histochemical studies.

An autopsy case of a malignant granular cell tumor of the right sciatic nerve was reported. The surgically excised malignant granular cell tumor of a 43-year-old women showed a close relationship with the sciatic nerve, and postmortem examination disclosed extensive metastases. Electron microscopic observations of the material obtained at surgery revealed many diagnostic granules containing myelin figures, as well as basement membrane-like structures around some of the tumor cells. Electron microscopic acid phosphatase staining suggested that the granules could be of a lysosomal origin. Immunohistochemical investigation showed the presence of S-100 protein in the tumor cells, a finding which is believed to be specific for the nervous system. And, focally, the cytoplasmic processes accompanied by basement membrane mimicked Schwann cells. This evidence suggested the highly malignant nature of the malignant granular cell tumor of probable Schwann cell origin.

Adult↗
PubMed1984

Glicentin-containing cells in intestinal metaplasia, adenoma and carcinoma of the stomach.

Glicentin-containing cells (Glic. cells) in intestinal metaplasia, adenoma and carcinoma of the stomach were examined using immuno-histochemical techniques. Glic. cells first occurred in the gastric mucosa of the transitional area between metaplastic and intact gastric glands. They frequently showed hyperplasia or micronoduli in the budding area of the deeper metaplastic glands, but in completely intestinalized mucosa these endocrine cells decreased remarkably. Gastric adenomas with mild dysplasia had a good number of glicentin-immunoreactive cells which were located in the deeper adenoma glands. Gastrin- and somatostatin-positive cells were also detected in the adenomas. The incidence of glicentin-positive tumor cells was significantly higher in well differentiated adenocarcinoma than in poorly differentiated adenocarcinoma. Among the seven cases of scirrhous argyrophil cell carcinoma, three showed glicentin- and glucagon-immunoreactivity in the same area of the tumor. These findings suggest that the selective increase of Glic. cells in intestinal metaplasia may be closely related to the development of gastric adenoma. Glicentin positive tumor cells in gastric carcinomas can be regarded to be an expression of intestinal or fetal markers.

Adenoma↗