Biomedical subjects
J Green
Publications and source records attributed to J Green.
Jejunal manometry patterns in health, partial intestinal obstruction, and pseudoobstruction.
The purpose of this study was to determine the utility of jejunal manometry in evaluating patients having partial small intestinal obstruction or the pseudoobstruction syndrome. We studied 10 healthy volunteers, 9 patients with documented mechanical obstruction, and 3 patients with chronic intestinal pseudoobstruction during fasting and for 2 h after a 650-kcal meal. Contractile activity, especially during phase 2, was extremely variable in all individuals studied. After the meal, the most striking finding in mechanical obstruction was the regular occurrence of clustered contractions. These were defined as 3-10 regular contractions, occurring 1 per 5 s, preceded and followed by at least 1 min of absent motor activity. The associated periods of quiescent motor activity may account for the unexpectedly reduced frequency and motility index in obstructed patients compared with normal subjects after the meal. Two different contractile patterns occurred in patients with pseudoobstruction. Before and after the meal, infrequent and low-amplitude contractions occurred in 2 patients, and multiple, aborally migrating, clustered contractions occurred in the other patient with pseudoobstruction. Manometric abnormalities occur in both mechanical obstruction and pseudoobstruction. However, the technique is probably not more sensitive than radiologic procedures, and the abnormalities that are detected by manometry are not specific. Some of the same motility changes occurred in both disorders.
Somatosensory cerebrally evoked responses in neurologic differentiation of organic vs. nonorganic impotence.
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Association between rising haemoglobin concentration and renal cyst formation in patients on long term regular haemodialysis treatment.
Seventeen of 20 (85%) patients on RDT for six to 13 years were found to have acquired renal cysts. There was a significant positive association between Hb and duration of RDT. The extent of cyst was positively and significantly correlated with the latest Hb and years on dialysis. There was also a significant positive correlation between size and years on dialysis only approached statistical significance. There was no correlation between kidney size and latest Hb. Complications in patients with acquired renal cysts appear to resemble those of familial polycystic disease with the added risk of neoplasia.
Effect of sulfinpyrazone upon antithrombin III and platelet factor 4 in chronic renal failure.
Despite the suppressive effects of uremia upon platelet function, patients with chronic renal failure are at increased risk for thrombotic diseases. This increased risk is linked to lowered concentrations of antithrombin III (AT III) and increased platelet release in vivo, as indicated by increased plasma concentrations of platelet factor 4 (PF4). Sulfinpyrazone, a drug capable of reducing the frequency of thrombotic events in uremic patients, allows return toward normal of the AT III while reducing to normal the plasma concentration of PF4. Thus, suppression of platelet function by sulfinpyrazone appears to reduce in vivo clotting, and thus reduces consumption of AT III.
Acyl-enzymes as thrombolytic agents in a rabbit model of venous thrombosis.
A derivative of human lys-plasmin in which the active site has been reversibly acylated (BRL 26920; p-anisoyl human lys-plasmin) has been examined as a fibrinolytic agent in a previously described rabbit model of venous thrombosis and shown to be significantly more active and less fibrinogenolytic than free plasmin. A p-anisoylated derivative of a streptokinase (SK)-activated plasmin preparation was significantly less fibrinogenolytic in vivo than the non-acylated enzyme. Acylation increased the fibrinolytic activity of preparations of SK-plasmin activator complexes. BRL 26921, the active site anisoylated derivative of the primary 2-chain SK-plasminogen complex was the most potent fibrinolytic agent studied. SK-Val442-plasminogen complexes, free or acylated, were biologically inactive in this model and confirm the essential nature of fibrin binding processes for effective thrombolysis in vivo.
[The syndrome of hyporeninemic hypoaldosteronism].
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[Treatment of electrolyte disorders].
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Chemotherapy of advanced ovarian carcinoma: initial experience using a platinum-based combination.
Thirty-five previously untreated patients with FIGO Stage III or IV ovarian carcinoma were treated with cis-diamminedichloroplatinum (II), Adriamycin and cyclophosphamide (PACe). Following four or five cycles of PACe, patients were assessed clinically for response. Twenty-one (60%) of the patients attained a clinical complete remission (CCR), seven (20%) a partial remission, and seven (20%) did not respond. The median survival of all patients was 19 months, 6.4 months for partial and nonresponders, and 26.5 months for clinical complete responders. Six patients remain relapse-free for more than two years. Patients entering into the study during the second year underwent a second-look procedure if they attained a CCR. Ten patients in CCR were assessed for second look; two refused, and eight underwent a laparotomy. Three of eight were in complete remission after second look, and a fourth patient who had microscopic disease in one ovary was rendered free of disease by surgery. Subjective toxicity was marked due to severe nausea and vomiting. Renal toxicity was not troublesome and myelosuppression was moderate. Ototoxicity and peripheral neuropathies were seen in seven patients. PACe is a highly effective induction regime but randomized studies are required to show that improved survival will result from it's use.
[Hyponatremia: mechanisms and treatment].
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Enzymatic sulfation of steroids. XV. Studies differentiating between rat liver androgen, estrogen, bile acid, glucocorticoid and phenol sulfotransferases.
Earlier reports left the number of enzymes that catalyzed phenol, androgen, estrogen, bile acid and glucocorticoid sulfation obscure. Here, we have utilized chromatographic, immunochemical and endocrinologic methods to compare and differentiate these enzymes in rat liver. Sulfotransferases I, II, and III--which sulfate glucocorticoids--were used in this comparison. We found that phenols were sulfated by phenol sulfotransferases 1 and 2, which were unrelated to the other enzymes studied here. Large amounts of phenol sulfotransferase 1 were found in both sexes. Large amounts of phenol sulfotransferase 2 were restricted to males. By contrast, the small amount of androgen sulfation found in both sexes appeared to be mediated by sulfotransferase II, which preferred 3 beta-hydroxysteroids, but also sulfated estrogens and glucocorticoids to lesser extents. The sulfation of estrogens presented a more complex picture. Most estradiol sulfotransferase activity in both sexes was due to an enzyme that sulfated estrone poorly, and did not sulfate the other steroids tested. This specific estradiol sulfotransferase was unrelated to the other sulfotransferases described here. Smaller amounts of estrogen sulfotransferase activity that sulfated estradiol and estrone equally well were present at concentrations dependent on the sex of test animals. This enzyme activity appeared to be due to sulfotransferases I, II and III. Most bile acid sulfotransferase activity eluted from DEAE-Sephadex A-50 columns with sulfotransferases I and II. However, data with males suggested that these enzymes were not responsible. Thus, phenols, androgens, estrogens and glucocorticoids were sulfated by six enzymes of differing substrate specificity: phenol sulfotransferases 1 and 2, specific estradiol sulfotransferase, and sulfotransferases I, II, and III. Unique bile acid sulfotransferases also appeared probable.
Characterization of a cell culture persistently infected with the DA strain of Theiler's murine encephalomyelitis virus.
We established a persistent infection in L 929 cells with the DA strain of Theiler's murine encephalomyelitis virus. Our studies showed that only a small number of cells in the cultures contained infectious virus or viral antigen. A role for interferon in the maintenance of persistence was suggested. Viral isolates from the cultures were not temperature sensitive, nor did they contain viral capsid polypeptide mutations or defective interfering particles. T1 oligonucleotide maps showed evidence of mutation in two of three isolates.
Interrelation of heart rate and autonomic activity in asymptomatic men with unobstructed coronary arteries. Studies with atrial pacing, adrenaline infusion, and autonomic blockade.
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Pulmonary function in normal humans with exercise and temperature-humidity stress.
Fifty-eight normal young male human subjects were exposed for 4 h to comfortable conditions (22 degrees C, 40% rh) or to heat stress conditions (30 degrees C, 60% rh) with or without exercise. Exercise amounted to two 15-min sessions of treadmill walking at 6.7 km X h-1 (4 mph) with a 10% grade beginning at 105 and 225 min after entry into the chamber. Measurements of 15 pulmonary function variables were made 1) before entry into the chamber, 2) 5 min after the first exercise period, 3) 5 min after the second exercise period, and 4) 24 h after the end of the exposure period. The exercise, estimated to use about two-thirds of the subjects' maximum oxygen intake, produced no statistically significant (P less than 0.01) changes in pulmonary function parameters. Heat stress produced significant changes in forced vital capacity, and possibly significant interactions were observed in peak expiratory flow and forced expiratory flow at 25% of vital capacity. Effects of the two factors appeared to be additive. Changes with exercise and heat stress were associated with reversal of a progressive decrease of airway resistance seen in subjects at rest in a comfortable environment.
O antigen loss by semi-rough E. coli causing recurrent urinary infections, analysed by gel column filtration and gas-liquid chromatography.
The surface polysaccharides of Escherichia coli isolated from 6 patients with recurrent urinary infection were studied serologically and chemically. In 4 patients multiple isolates were obtained. The first isolate from each of these patients was a smooth strain which could be serotyped with O antisera. The second isolates from 3 of these patients, obtained at intervals of 1-6 wk, were semi-rough strains. They could not be serotyped after culture on nutrient agar but could be serotyped after subculture on blood agar. In each patient the serotype remained the same. Gel column filtration of the polysaccharides, and gas-liquid chromatography (GLC) of the trimethyl-silylated sugars showed that smooth strains carried a preponderance of O-specific carbohydrate with little core carbohydrate. The semi-rough strains had lost nearly all their O-specific carbohydrate. The change from smooth to semi-rough involved the progressive loss of full length O-specific side-chains rather than a widespread 'shortening' of these side-chains. In patients chronically infected with the same strain of E. coli it has been shown that GLC analysis of the carbohydrates of different isolates of the same strain in one patient will vary because often the strain will undergo a smooth to semi-rough or rough variation. This variation indicates that GLC analysis of extracted carbohydrates would often not be able to determine whether multiple isolates of E. coli from one patient were the same or different strains.
Adult Still's disease associated with pregnancy.
A 23-yr-old woman in her sixth month of pregnancy presented with the systemic variant of juvenile rheumatoid arthritis (Still's disease). The symptoms included high fever, erythematous evanescent maculopapular rash, hepatosplenomegaly and asymmetric arthritis. During a follow-up period of 1 yr, anti-inflammatory drugs (both steroidal and nonsteroidal) were administered, leading to the eventual disappearance of the patient's complaints. Adult-onset Still's disease reported in the literature is reviewed, and the association of Still's disease with pregnancy is discussed.
Passage of viral membrane proteins through the Golgi complex.
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