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Biomedical subjects

J Gougeon

Publications and source records attributed to J Gougeon.

52 records · Page 3Linked to original sources
PubMedDate not supplied

Detection and measurement of rheumatoid factor using a new immunoenzyme technique with peroxidase/anti-peroxidase complex.

A new, solid-phase enzymatic technique is proposed for the detection and measurement of rheumatoid factors (RF). A complex (PAP) consisting of peroxidase and rabbit IgG anti-peroxidase antibodies was used as antigen. The sensitivity of the technique is such that even "physiological" levels of RF may be detected in the majority of serum samples. The intra-assay and inter-assay repeatability (6-8% and 12%) is better than that for either of the traditional agglutination techniques (latex fixation test and Waaler-Rose reaction, or LWR) and this means that the serological evolution of the illness may be followed more accurately for each patient. The detection of non-agglutinating RF was also possible. The antigen used (i. e. rabbit IgG) was fixed immunologically to the enzyme, hence it was not denatured by chemically labelling. This antigen resembles that of the Waaler-Rose reaction. A variation of this method enabled the detection of RF immunoglobulin class, when only the IgM fraction of the sera tested could be fixed to the solid phase by means of anti-mu F(ab')2 antibody before introduction of the antigen. The affinity constant could be measured without antibody purification. In 211 cases of adult rheumatoid arthritis, abnormally high levels of RF were revealed in 78% of the subjects using the PAP technique and in only 53% with the agglutination (LWR) techniques. On the other hand, the RF levels were within normal limits in the 51 cases of other inflammatory rheumatisms lacking RF according to LWR techniques, in 62 cases of non-inflammatory diseases (hip osteoarthrosis, low back pain) and also in the 42 cases of other inflammatory diseases.

Animals↗
PubMedDate not supplied

[A case of Whipple's disease with ankylosing spondylarthritis treated with trimethoprim-sulfamethoxazole].

We report the case of a patient, suffering from Whipple's disease and HLA B27 positive ankylosing spondylitis with syndesmophytes and erosive discopathy. Since spinal radiographic aspects of spondylitis due to Whipple's disease are unusual, we are debating on their relation. We, then, took an interest in the treatment given to this patient: trimethoprim-sulfamethoxazole which would now appear to be the best antibiotic for Whipple's disease.

Aged↗
PubMedDate not supplied

[The lateral atlantoaxial joint involvement in the rheumatoid arthritis. Report of ten cases and review (author's transl)].

An analysis of ten recent observations of lateral arthritis of C1-C2 during the course of rheumatoïd arthritis. This lesion, which is not rare, has charactéristic symptoms: localized pain together with occipital (Arnold's) neuralgia and specific blocking of head rotation towards the afflicted side. Frontal per-oral radiography shows joint line lesions most often unilateral, and a displacement of C1 towards the non afflicted side with pseudoinclination of the odontoïd process towards the afflicted side. Sometime more destructive lesions of the lateral masses of the atlas give them a triangular form and this may result eventually in an upwards "vertical" displacement of the odontoïd. The pain usually subsides in a few weeks after a conservative treatment with or without temporary immobilisation by a light removable cervical collar but the loss of the homolateral rotation of the head persists. Besides occipital (Arnold's) neuralgia, there seems to be no other neurologic complications; if they follow, it is due to an associated anteroposterior displacement.

Adult↗