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Biomedical subjects

J G Gall

Publications and source records attributed to J G Gall.

104 records · Page 6Linked to original sources
PubMed1970

Chromosomal localization of mouse satellite DNA.

Hybridization of radioactive nucleic acids with the DNA of cytological preparations shows that the sequences of mouse satellite DNA are located in the centromeric heterochromatin of the mouse chromosomes. Other types of heterochromatin in the cytological preparations do not contain satellite DNA.

Animals↗
PubMed1969

Formation and detection of RNA-DNA hybrid molecules in cytological preparations.

A technique is described for forming molecular hybrids between RNA in solution and the DNA of intact cytological preparations. Cells in a conventional tissue squash are immobilized under a thin layer of agar. Next they are treated with alkali to denature the DNA and then incubated with tritium-labeled RNA. The hybrids are detected by autoradiography. The technique is illustrated by the hybridization of ribosomal RNA to the amplified ribosomal genes in oocytes of the toad Xenopus. A low level of gene amplification was also detected in premeiotic nuclei (oogonia).

Animals↗
PubMed1969

Molecular hybridization of radioactive DNA to the DNA of cytological preparations.

A method is presented for detecting the cellular location of specific DNA fractions. The technique involves the hybridization of a radioactive test DNA in solution to the stationary DNA of a cytological preparation. Sites of DNA binding are then detected by autoradiography. Experiments with DNA of the toad Xenopus are described.

Animals↗
PubMed1967

Octagonal nuclear pores.

Negative staining of isolated nuclear envelopes by phosphotungstate shows that the nuclear pores are octagonal rather than circular. Pores of the same shape and approximately the same width, 663 +/- 5 A, were demonstrated in the newt, Triturus, the frog, Rana, and the starfish, Henricia. The outer and inner diameters of the annulus associated with each pore are respectively greater and less than the width of the pore itself. For this reason surface views of the envelope, unless negatively stained, fail to show the true dimensions of the pores.

Animals↗