Attachment of Escherichia coli to human spermatozoa.
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Biomedical subjects
Publications and source records attributed to J Friberg.
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Real-time sonography was used in a prospective study of 32 patients as the sole diagnostic parameter for ectopic pregnancy. The minimal finding for diagnosis was an identifiable gestational sac with a circular pattern of echoes. Neither the presence or absence of an intrauterine sac nor the knowledge of either a positive or negative pregnancy test was used in the diagnosis. Among the 32 study patients, the diagnosis of ectopic pregnancy was made by sonography in nine (28.4%). Follow-up surgery confirmed the diagnosis in eight (89%). Among 23 patients with negative findings on sonography, only one was found on follow-up to have an ectopic pregnancy, for a 96% diagnostic accuracy for the negative group. Thus, of 32 patients with the potential diagnosis of ectopic pregnancy, 30 (94%) were correctly diagnosed using real-time sonography alone.
Postcoital tests (PCTs) were examined from couples where the husbands had circulating head-to-head (H-H) or tail-to-tail (T-T) sperm-agglutinating antibodies. The results were compared with findings in couples without antibodies. The presence of H-H sperm-agglutinating antibodies did not interfere with the outcome of the PCT. High serum titers of T-T sperm-agglutinating antibodies, the presence of such antibodies in the seminal fluid, and/or a strong or complete autoagglutination in the husband's ejaculate markedly reduced the number of invading spermatozoa as well as their survival in the PCT. A moderate titer of T-T sperm-agglutinating antibodies was also reflected in the PCT but low titers did not have any influence on the results.
Repeated postcoital tests (PCTs) were evaluated in couples with unexplained infertility, in couples with circulating sperm-agglutinating antibodies of the head-to-head (H-H) or tail-to-tail (T-T) type in serum, and in couples without any antibodies. The results were compared with those of PCTs in the conception cycle from women undergoing gonadotropin treatment for anovulation. Only limited differences in the PCT results were noted between couples with sperm-agglutinating antibodies, couples without such antibodies, and fertile couples.
Thirty-four semen samples from fertile men were subjected to bacteriologic evaluation before and after freezing to -196 degrees C using 10% glycerol as the protective medium. Cultures of the fresh portions from all ejaculates examined showed the presence of aerobic bacteria. Staphylococcus epidermidis, Corynebacterium sp., alpha-streptococci, and nonhemolytic streptococci were found so commonly that they were considered commensals. Enterococci, Escherichia coli, S. aureus, and beta-hemolytic streptococci group B, as well as Pseudomonas aeruginosa, P. maltophilia, and P. fluorescens, were also recovered from a few semen samples of these fertile men. Practically all bacteria isolated from the fresh ejaculate were also recovered from the portions frozen in liquid nitrogen.
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Serum and seminal fluid from infertile men with head-to-head (H-H) or tail-to-tail (T-T) sperm-agglutinating antibodies and fertile men devoid of sperm antibodies were examined for immunoglobulin (Ig)G, IgA, IgM, IgD, and IgE. Only IgG, IgA, and IgE were demonstrated in the seminal fluid. No statistically significant differences in the serum or seminal fluid immunoglobulin concentrations were found between the fertile and the two infertile groups of men examined.
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In 10 infertile men with sperm-agglutinating antibodies in serum and a history of inguinal herniorrhaphy the site of the previous operation was explored. Five of the men had an occlusion of the vas deferens and in three others spermatoceles were noted in the epididymis. The occlusion of the vas deferens was in the area of the previous herniorrhaphy. Infertility caused by the development of sperm antibodies may occasionally be a long-term consequence of inguinal herniorrhaphy.
Three different species of mycoplasmas--M. hominis, M. fermentans, and Ureaplasma urealyticum--have been found to infect human genitalia. In the man, mycoplasmas appear to play a role in the etiology of nonspecific urethritis and prostatis. Mycoplasmas repeatedly have been cultured from the tubes of patients with acute salpingitis and a significant antibody response to the organisms during recovery has been reported. Obstetric infections and puerperal sepsis occasionally seem to be caused by mycoplasmas. These organisms also appear to induce spontaneous abortion as well as cause infertility in a small proportion of couples.
Weak or sometimes up to moderate spermagglutination was encountered in ejaculates from a group of men without spermagglutinating antibodies in serum. A similar pattern of spermagglutination was seen in ejaculates from men with head-to head (H-H) spermagglutinating antibodies in serum, even when a high titer (greater than or equal to 1:64) of antibodies was present. In contrast, men with high titers of tail-to-tail (T-T) sperm agglutinating antibodies in serum showed very marked or complete spermagglutination in the ejaculates. The agglutination in ejaculates from men with low titers (less than or equal to 1:32) of T-T spermagglutinating antibodies in serum was much less conspicuous and equaled that in ejaculates from men without such antibodies. This indicates that only a high titer of T-T spermagglutinating antibodies is associated with marked or complete spermagglutination in ejaculates.
A comparison between freezing of human sperm using glycerol or glycerol egg-yolk citrate is presented. Freezing was performed with a gradual lowering of the temperature using alchol-liquid nitrogen or by immersion into liquid nitrogen. Great variations were observed within the same donor sperm sample when the sperm was frozen according to different techniques and marked post-thaw, motility variations were also found between spermatozoa from different donors. No statistically significant differences were found in the number of pregnancies obtained following inseminations with sperm frozen according to the two methods.
A series of determinations of the concentrations of trimethoprim in seminal fluid and serum was made during the treatment of 18 men. Trimethoprim could be demonstrated in an average concentration in serum of 1.8-2.0 mug per millilitre and in seminal fluid of between 1.0 and 1.5 mug per millilitre. At the same time it was demonstrated that the combination of trimethoprim and sulphamethoxazole (co-trimoxazole) has no effect on genital mycoplasma species.
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The relationship between the state of the germinal epithelium and the type and titer of circulating sperm-agglutinating antibodies has been investigated in a series of 59 azoospermic or occasionally cryptozoospermic men. The patients were grouped according to the condition of the germinal epithelium as observed from testicular biopsy specimens, as well as to type and titer of circulating sperm-agglutinating antibodies investigated by a previously described microagglutination technique. Evidence is presented to suggest that the presence of mature spermatozoa in the testicular structures may be a prerequisite for the spontaneous production of circulating sperm-agglutinating antibodies, at least of the head-to-tail (H-T) agglutinating type. Furthermore, these circulating H-T sperm-agglutinating antibodies, once they are formed, do not seem to interfere adversely with the germinal epithelium of the carrier.
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