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Biomedical subjects

J Franz

Publications and source records attributed to J Franz.

At least 109 records · Page 6Linked to original sources
PubMed1982

[Detection of antibodies against the virus causing viral gastroenteritis in swine using the modified autoradiography test].

A modified method for autoradiographic determination of antibodies to the virus of transmissible gastroenteritis of swine was developed. It is based on the actual reaction between antigen bound in cells of the infected cell cultures and antibodies in tested sera, which is visualized, by help of rabbit antibodies marked 125J (125J RaSw IgG antibody) to porcine IgG, on a sensitive radiograph and evaluated on the basis of darkening at the point of positive immunological reaction. Specificity of the test and mutual comparability and reproducibility of the results were confirmed by examining the known positive and negative sera and by comparison with the results of the virus-neutralisation test. Out of the 36 examined porcine blood sera the antibodies were proved autoradiographically only in the samples positive also by virus-neutralisation. In experimentally infected pigs, the same dynamics of antibody production was recorded by the two tests. They were, however, demonstrated autoradiographically already by the two tests. They were, however, demonstrated autoradiographically already the eighth day after infection, while by virus neutralisation test as late as 14th day. Their level increased gradually until 35th day after infection.

Animals↗
PubMed1981

Detection of antirotavirus immunoglobulins A, G, and M in swine colostrum, milk, and feces by enzyme-linked immunosorbent assay.

An enzyme-linked immunosorbent assay was developed to allow direct detection of class-specific antirotavirus antibodies. In colostrum and in milk, antirotavirus antibodies were found in the three immunoglobulin classes. Antirotavirus immunoglobulins G and M were predominant in colostrum, whereas antirotavirus immunoglobulin A was predominant in milk and feces.

Animals↗
PubMed1981

Measurement of porcine faecal IgA, IgG and IgM levels by a competitive enzyme-linked immunosorbent assay.

The concentrations of IgG, IgA and IgM were compared in faecal material of healthy and diarrhoeic pigs using a competitive enzyme-linked immunosorbent assay (ELISA). The relative concentrations of faecal immunoglobulins (Ig) in healthy piglets and adults were significantly different (P less than 0.01). IgA was the most important Ig in adult faeces while IgM was predominant in piglet faeces. IgG appears to be a relatively rare component. No significant differences could be detected between groups of healthy and diarrhoeic piglets. In adult faeces, significant changes (P less than 0.02 to P less than 0.001) in Ig relative concentrations were observed between diarrhoeic and healthy animals.

Aging↗
PubMed1980

Use of fluorescein conjugated staphylococcal protein-A as a labeling agent for porcine lymphocytes.

Staphylococcal Protein-A conjugated to fluorescein (FPA) was compared to a fluorescein conjugated sheep immunoglobulin anti-pig IgG (FSAPG) as a labeling agent for surface IgG positive (sIg+) porcine lymphoid cells. At plateau concentration of the reagents, more lymphoid cells were labeled with FPA than with FSAPG. However, on a protein concentration basis, FPA was less sensitive than FSAPG. As a control, FPA proved to be a poor labeling agent for sIg+ bovine lymphoid cells when compared to FITC conjugated rabbit IgG anti-bovine IgG (FRABG). Adsorptions of porcine lymphoid cells onto Protein-A Sepharose were performed in order to study the differential specificity of the Protein-A for sIg and for free immunoglobulins. The results of such absorptions showed that Protein-A Sepharose, whatever its affinity for Fab fragments from porcine immunoglobulins, could be used to enrich the sIg negative lymphoid cell populations in the pig.

Animals↗
PubMed1977

[Effect of different systems of colostral nutrition on the level of immunoglobulins in the blood of calves].

New-born calves, artificially fed colostrum or native colostral whey, either dried or preserved by another method, had good health and good weight gains (between 0.05 and 0.60 kg). No greater differences were observed between the groups of calves given three times the colostrum of their mothers, calves given mixed colostrum, and calves fed colostral whey powder. In all groups only individual differences in IgG content in the blood serum were observed after 48 hours from birth. Hypogammaglobulinaemia occurred in individual cases both in calves given small amounts of colostrum or colostral whey and in calves given sufficient quantities. The time that had elapsed from birth to the first drinking did not exert any greater influence upon the IgG level in the blood; the decisive factor was the amount of colostrum taken in by the calf in the first dose. The rate of the absorption of IgG1, IgG2, IgA, and IgM from colostrum and the concentration of the immunoglobulins in the serum depended on the quantity of colostrum in the first dose and were not influenced to any greater degree by the amount of colostrum given to the calves in further doses. The amount of IgG in the blood serum of calves corresponded approximately to the level of colostral antibodies to the virus PI-3. The antibodies to the virus PI-3 and small quantities of IgG were observed also in the serum to new-born calves before drinking colostrum.

Agammaglobulinemia↗
PubMed1977

[Attempt at the prevention of respiratory syndrome by rearing calves with a minimum morbidity rate].

Under the conditions of a pilot experiment on two farms the authors tested the system of calf rearing in which the calves were separated from their mothers immediately after birth to be kept in specially equipped rearing facilities. In calf-house K with a semi-continual system of operation the total number of calves reared on milk nitrition was 959 and the average daily weight gain in individual groups (41 to 75 days from birth) ranged, with the exception of four groups, from 0.50 to 0.78 kg, depending on the deterioration or improvement of the conditions of operation, tending, or hygiene, In calf-house D with the all-in all-out system of operation where 391 calves were reared, the average daily weight gain in individual groups (55 to 84 days from birth) was 0.70 to 0.87 kg, with the exception of four groups. The calves reared in this way maintained good growth ability also in fattening, showing the average weight gain of 1.00 kg and 1.08 kg in groups transferred from the vegetable-nutrition department of the houses to a disinfected stable, in comparison with the 0.77 kg and 0.85 kg gains in groups, transferred to stables with the continual system of operation. Heifers were ready for the first insemination at the age of 20 to 23 months. The method of calf-rearing without mothers, based on controlled supply of colostrum to calves reared in closed populations in clean environment disinfected in advance, enables the production of "minimum-morbidity animals" giving excellent results in all parameters of production.

Animal Feed↗