Search PubMed⌕ Search

Biomedical subjects

J Fleurette

Publications and source records attributed to J Fleurette.

At least 163 records · Page 9Linked to original sources
PubMed1984

[Evaluation of the incidence of serological cross reactions between Legionella and Mycoplasma or Chlamydia].

Because of the possible serological cross reactions frequently described in the literature between L. pneumophila and M. pneumoniae or between L. pneumophila and Chlamydia, we decided to determine their frequency with our sera and our own methods. The anti M. pneumoniae and Chlamydia antibodies were researched in 162 sera samples from 46 cases of legionellosis serologically diagnosed and in 51 sera from 26 hyperimmunised rabbits. Conversely, anti L. pneumophila antibodies were researched in 165 sera from mycoplasmosis (61) or chlamydiosis (25 cases). None of the sera from mycoplasmosis or chlamydiosis tested by IFA and MA had antilegionella antibodies. None of the rabbits' sera hyperimmunised with Legionella had anti M. pneumoniae or Chlamydia antibodies. On the contrary, by complement fixation and IFA, out of the 46 cases of legionellosis under study, 6 showed a seroconversion in M. pneumoniae and 2 in Chlamydia. The Ig class determination did not show IgM titers in 7 of these 8 sera. The mechanisms of these cross reactions are discussed: antigens and methods used, booster effect during legionellosis, concurrent mycoplasmosis or chlamydiosis.

Agglutination Tests↗
PubMed1984

[Antibiotic susceptibility testing by the disk method. Comparative evaluation of 5 commercial systems].

The systems studied were: bioMerieux, Difco, Institut Pasteur Production, Oxoid (media, discs, standards of interpretation). Each batch was tested for pH value and calcium and magnesium concentrations. The activity of 19 antibiotics was determined by use of three reference strains (E. coli ATCC 25922, S. aureus ATCC 25923, P. aeruginosa ATCC 27853). The most suitable inoculum was defined after testing three different methods of preparation. The susceptibility of each strain to each antibiotic was studied 30 times using each system. The reproducibility of the diameters with each system and the intersystem variance was calculated. This allowed groups of homogeneity between the different systems to be defined. The results were interpreted according to the various existing standards (manufacturer, French committee of SFM, WHO) yielding an overall discordance of 0,29%, of little practical importance. Finally the reliability of each system was evaluated according to WHO, data. The Difco medium gave the most accurate results with the other media tending to produce excessively large zones of inhibition.

Anti-Bacterial Agents↗
PubMed1984

[Effectiveness of 9 soaps and/or antiseptics on hand flora after surgical-type washing].

We studied the effect of nine soaps and/or antiseptics on the bacterial flora of hands 5 minutes after a surgical scrub. Each agent was used by ten healthy volunteers, free of skin lesions. The following agents were used: chlorhexidine gluconate 4% and 1.5%, povidone iodine 4%, ethanol 70 degrees, isopropanol 70 degrees, a non-antiseptic soap, and another soap followed by either ethanol 70 degrees, isopropanol 70 degrees or a preparation containing H2O2. The surgical scrub procedure varied slightly according to whether or not the agent was soapy and required rinsing. Sampling was carried out using Gaschen's bag method with 400 ml of neutralising solution. Counts were made after 48 hours aerobic incubation at 35 degrees C on tryptic soy agar with 1% Tween 80, and after 8 days anaerobic incubation at 35 degrees C on Brewer's yeast agar with 1% Tween 80. Results were expressed as the log 10 to the number of bacteria per hand. Statistical significance was determined using the Student t test. The greatest reduction in aerobic flora was produced by isopropanol 70 degrees C (1.7 log 10). 1.5 to 0.5 log 10 reductions were produced, in the following decreasing order, by ethanol 70 degrees, povidone iodine, chlorhexidine gluconate 4% and 1.5% and a soap with ethanol 70 degrees. A reduction of less than 0.5 log 10 was produced by a soap with isopropanol 70 degrees, and soaps with H2O2. Similar results were obtained with the facultative anaerobes.

Anti-Infective Agents, Local↗
PubMed1983

[Bacteriologic study of 125 cases of infectious streptococcal endocarditis].

Streptococci remain the bacterial group the most frequently associated with microbial endocarditis. This paper points out the ability of the different species to cause endocarditis. 128 streptococci are isolated from patients with microbial endocarditis (127 from blood cultures, 1 from a valve). Isolates are speciated by biochemical tests and the determination of their group antigen. The viridans streptococci most frequently isolated are S. sanguis I (27 p. cent), S. mitis (16,5 p. cent) and S. sanguis II (9,6 p cent). A comparative survey of the results is rather difficult due to the methodological and taxonomical divergences.

Endocarditis, Bacterial↗
PubMed1983

Comparison of microagglutination with the indirect immunofluorescence assay for the diagnosis of infection with Legionella pneumophila serogroup 1.

A comparison was made of the indirect immunofluorescence assay and microagglutination in the diagnosis of infections caused by Legionella pneumophila serogroup 1. Control sera consisted of 709 sera from patients without pneumonia and 99 sera from patients with Mycoplasma or Chlamydia infection. The 468 test sera were from 51 patients with serologically confirmed or suspected Legionella pneumophila serogroup 1 infection, and from 230 patients with pneumonia of unknown aetiology. There was good agreement between the results of the two methods for detection of antibodies to Legionella pneumophila serogroup 1. However, contrary to what is currently reported in the literature, microagglutination was the more sensitive method in this study, especially if the first serum samples were compared. The detection of IgM by microagglutination probably explains this increased sensitivity.

Agglutination Tests↗
PubMed1983

Enterotoxin production, phage typing and serotyping of Staphylococcus aureus strains isolated from clinical materials and food.

The production of enterotoxins A, B, C and F by strains of Staphylococcus aureus isolated from various clinical sources and from isolates implicated in food poisoning was investigated. One hundred and ninety one of the 374 clinical strains (51.1%) were found to be enterotoxigenic; of these, 81 (27.7%) strains produced enterotoxin A, 57 (15.3%) strains produced enterotoxin B, 23 (6.2%) strains produced enterotoxin C, and 64 (17.1%) strains produced enterotoxin F. These enterotoxigenic strains were most frequently lysed by phages of group III (21.5%) or were not typable (22%). Eighteen of the 29 strains implicated in food poisoning were enterotoxigenic. The correlation of antigens and bacteriophage patterns with enterotoxigenicity was determined: enterotoxin A being related to a4 antigen, enterotoxin B to phages of 94/96 complex with c1, o antigens, and enterotoxin F to phages of group I with 2632, k1k2, m antigens.

Bacterial Toxins↗
PubMed1983

Modified method for production and purification of Staphylococcus aureus enterotoxin B.

A medium containing 4% bio-trypcase and 1% yeast extract was used for the production of Staphylococcus aureus enterotoxin B. The yield obtained was estimated at 200 micrograms of enterotoxin per ml of S. aureus S-6 culture supernatant. The purification method involves chromatography on Biorex 70 resin, isoelectric focusing, and gel filtration on Sephadex G-100. The purified enterotoxin (isoionic point, pH 8.55) was shown to be homogenous protein with a molecular weight of 29,000 when tested by gel electrophoresis.

Chromatography, Gel↗
PubMed1983

Biotypes, serotypes and phage types of caprine strains of Staphylococcus aureus.

Nine of 11 caprine strains of Staphylococcus aureus examined belong to biotype C. They were typable serologically using antisera developed for typing human strains of Staphylococcus aureus. H2 was found to be the dominant thermostable antigen whilst 1 and 0 were the most common thermolabile antigens. Some strains possessed protein A and poly A beta (beta-glucosaminyl ribitol teichoic acid). Phage typing of the strains was achieved with an international set of phages for typing human strains of Staphylococcus aureus and they belong to phage groups II, and the mixed group.

Animals↗
PubMed1983

[In vitro antibacterial activity of cefmenoxime (SCE 1365)].

617 clinical isolates were tested, 592 of which were from hospital source. The minimal inhibitory concentrations of cefmenoxime were determined by a microtiter dilution method, using Mueller-Hinton broth. The results obtained give a 92% agreement with the reference agar-dilution method. Cefmenoxime shows a potent activity against Enterobacteriaceae (n = 420): the modal MIC is less than or equal to 0,03 mg/l, and 90% of them are inhibited by 1 mg/l. Some isolates require a higher concentration, above 32 mg/l: Enterobacter (8%), indole-positive Proteus (13%), Serratia (3%), Citrobacter (3%). Pseudomonas (n = 71) and Acinetobacter (n = 62) appear to be less susceptible than Enterobacteriaceae. The modal MIC is 16 mg/l and the concentration inhibiting 90% of the isolates is above 32 mg/l. The modal MIC of cefmenoxime against Staphylococcus aureus (n = 64) is 1 mg/l, and 90% of the strains belonging to this species are inhibited by 32 mg/l.

Acinetobacter↗
PubMed1983

[In vitro effect of rosoxacin on hospital bacteria. Results of a multicenter study].

This work reports a multicenter study of antibacterial activity of rosoxacin, a new antibacterial agent of the quinolone group. Enterobacteriaceae are very sensitive to rosoxacin (modal MIC 0,5 micrograms/ml); resistant strains are observed in all species, but more often among Serratia and Citrobacter. Pseudomonas aeruginosa is less sensitive with a maximum number of strains between 2 and 8 micrograms/ml, so it is nearly for Acinetobacter. Haemophilus are very sensitive, having MIC of 0,03 and 0,06 micrograms/ml. The spectrum of rosoxacin includes Gram positive bacteria, since the MIC of Staphylococci are similar to Enterobacteriaceae; Enterococci are less sensitive, the major part of the strains being inhibited by 4 and 8 micrograms/ml.

4-Quinolones↗
PubMed1983

[Growth of Staphylococcus aureus Cowan I in the presence of subbactericidal concentrations of povidone-iodine].

Staphylococcus aureus, strain Cowan I, is cultivated in the presence of povidone iodine (PVP.I) at the concentration of 0, 640, 960 micrograms/ml; growth curves points are platted (OD 640 mm) at 0, 1, 2.50, 3, 4.50, 7 and 24 hours. Growth curves in the presence of povidone alone as well as in the standard without antiseptic are similar and reproducible. A bacteriostatic effect is observed with 640 micrograms/ml PVP.I concentration compared to an incomplete bactericidal effect with 960 micrograms/ml concentration. Proteinee A detected by conditioned hemagglutination method is present in the centrifugation pellet of the standard and only in the supernatant of the test samples during the early phases of the growth. Production of alpha-ribitol-teichoic acid detected by counterimmunoelectrophoresis is not significantly different in the standard and in the presence of antiseptic. Biochemical characteristics studied by Api Staph gallery are identical for the control strain and the strain grown in the presence of PVP.I. Observed by scanning and transmission electronic microscope the bacterial cells do not present structural modification during growth with PVP.I.

Povidone↗
PubMed1983

[Antibacterial activity of apalcillin against 300 strains of enterobacteria].

The in vitro bacteriostatic activity of apalcillin was compared with those of piperacillin, mezlocillin and carbenicillin. The minimal inhibitory concentrations determined by a microtiter dilution method, show a bi-modal distribution: apalcillin, piperacillin: 1 mg/l - greater than 128 mg/l; mezlocillin: 2 mg/l - greater than 128 mg/l; carbenicillin: 4 mg/l - greater than 128 mg/l. Two thirds of the isolates are inhibited by 8 mg/l of any antibiotic, except carbenicillin (128 g/l). Two genus seem less susceptible: Klebsiella display modal MIC of apalcillin and piperacillin equal to 4 mg/l and above 128 mg/l: Serratia isolates resist to 128 mg/l, whatever antibiotic is considered. The in vitro bactericidal activity of apalcillin was evaluated by killing-curves method against 8 Enterobacteriaceae strains. Used at a concentration equal to the MIC or twice the MIC, apalcillin produces a 1,5-2 log10 reduction in viable count, 3 hours after the addition of antibiotic.

Ampicillin↗
PubMed1983

[Multicenter study of the in vitro antibacterial activity of ceftazidime on gram-negative bacilli].

This work reports a multicenter study of antibacterial activity of ceftazidime, a new third generation cephalosporin, on hospital Gram negative rods. Enterobacteriaceae are very sensitive to ceftazidime with a maximum number of strains inhibited by 1 microgram/ml or less (modal MIC 0, 125 microgram/ml); some resistant strains are observed, particularly among Enterobacter and Citrobacter. Activity of ceftazidime on Pseudomonas aeruginosa is superior to other third generation cephalosporins; modal MIC is 1 microgram/ml and 88% of the strains are inhibited by 4 microgram/ml or less. Acinetobacter are less sensitive to ceftazidime, with a modal MIC of 8 microgram/ml.

Bacteria↗