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J Fleurette

Publications and source records attributed to J Fleurette.

At least 181 records · Page 10Linked to original sources
PubMed1983

[Effect of storage time at -20 degrees C on the determination of serum concentrations of tobramycin in the presence of 6 beta-lactams].

We assessed the role of six beta-lactam antibiotics and of storage time at - 20 degrees C, in inactivation of tobramycin in patients' sera. Several ranges of concentrations were used for the combined antibiotics. The tobramycin concentrations were measured both by microbiological assay (plate diffusion) and an enzyme mediated immunoassay technique (EMIT). Using the bioassay method, the results after 8 days of frozen storage were as follows: carbenicillin and ticarcillin (256 mg/l) induced a 40-50% reduction of tobramycin activity, whereas mezlocillin (256 mg/l) had less effect: a 15-20% reduction. After 15 days at -20 degrees C the results were nearly the same except for azlocillin (256 mg/l), increasing its percentage reduction to 20%. The results obtained by EMIT procedure were significantly better after 8 days of frozen storage: only mezlocillin (5% reduction) and azlocillin (10%) were effective. Nevertheless after 15 days at -20 degrees C, the inactivating action of the pre-cited antibiotics were similar to those obtained at the same time with the bioassay method. Piperacillin and cefsulodin never induced any reduction of tobramycin levels, whatever the time of storage or quantification procedure used. So, our opinion is that patients' sera containing a beta-lactam antibiotic in combination with tobramycin should be assayed immediately upon receipt. Keeping it at -20 degrees C is not sufficient to prevent in vitro tobramycin inactivation.

Anti-Bacterial Agents↗
PubMed1983

[Value of the study of fibrinogen affinity in the identification of Staphylococcus aureus. Results of a multicenter study].

The passive haemagglutination reaction for detecting the Staphylococcus aureus fibrinogen affinity was done in a collaborative study. The test was compared to the major tests used in medical microbiology. The test was as efficient as the protein A or coagulase tests. Its simplicity, rapidity and specificity make it a useful tool for the diagnosis of this species.

Fibrinogen↗
PubMed1983

Micromethod for carbon substrate assimilation by Pseudomonas maltophilia.

Assimilation of 60 carbon sources by 114 Pseudomonas maltophilia strains, identified by conventional methods, was studied by a standardized micromethod (API strip). Automatic reading of growth intensity was done at 1, 2 and 4 days. Growth kinetics of reference strain NCTC 10257 were also studied and showed evidence of different growth according to the substrates. The results agreed well with nutritional patterns reported. This micro-method seems suitable for daily use by laboratories because of purity and standardization of substrates, facility of use, accurate and automatic reading, and the great number of assimilation tests possible.

Amino Acids↗
PubMed1983

[Legionella infections].

The authors review the bacteriological features of Legionella infections, their clinical symptoms and the methods of diagnosis. They stress the unusual ecological features of Legionella which generally lives in natural water reservoirs or in artificial reservoirs (drinking water piping, air conditioning). In the clinical situation, current emphasis is on the extrapulmonary infections whose pathogenesis has not yet been fully explained. The incidence and prevalence of Legionella in pneumonia still needs to be defined. The methods of bacteriological and serological diagnosis are described as well as the ways of interpreting the results.

Animals↗
PubMed1982

[Comparative in vitro activity of dibekacin, gentamicin and tobramycin on 617 bacterial strains].

Minimum inhibitory and bactericidal concentrations of 3 aminoglycosides (dibekacin, gentamicin, tobramycin) have been recorded with 617 hospital strains. 4 mg/l of tobramycin inhibited 82 to 84% of Enterobacteria, Pseudomonas, Acinetobacter and S. Aureus; the less sensitive species are Serratia (51%) and Citrobacter (68%). 4 mg/l concentration of gentamicin or dibekacin inhibited 80-83% of Pseudomonas and S. Aureus, 78-79% of Acinetobacter and 74% of Enterobacteria. Dibekacin shows an in vitro activity superior to that of gentamicin and inferior to that of tobramycin on Klebsiella and Proteus mirabilis, but is rather to that of gentamicin and tobramycin on all other bacteria species.

Anti-Bacterial Agents↗
PubMed1982

[Skin flora of the hands, elbow-hollow and fore-arm (author's transl)].

During five years, the values of the skin flora of the hands, fore-arm and elbow-hollow were studied on volunteers, especially five of them. Samples were taken according to the washing method. Results show important quantitative variations of the flora between the differents volunteers and even for a same volunteer. In the five long term followed subjects, the differences were: 2.17 log 10 for aerobic flora and 1.97 log 10 for aeroanaerobic flora of the hands, 3.90 log 10 for aerobic flora and 3.88 log 10 for aero-anaerobic flora of the fore-arm, 2.95 log 10 for aerobic flora and 3.35 log 10 for aero-anaerobic flora of the elbow-hollow. These variations remained independent of the season. According to these variations, already notified by others authors it is suggested first to standardize sampling and bacteriological methods, second to develop multicentric programs in view to increase the number of subjects to be included in the study.

Adult↗
PubMed1982

[Diseases of the lungs caused by legionella species (author's transl)].

Ever since the 1976 Philadelphia epidemic and the isolation of the causative organism by MacDade in 1977, numerous clinical, epidemiological and bacteriological papers have stressed the significance of the "new" causative organisms responsible for serious cases of pneumonia. On the basis of knowledge available at present, the Legionellaceae family accounts for these bacteria. There are five different species in the genus Legionella: L. pneumophila, L. micdadei, L. bozemannii, L. dumoffii and L. gormanii. L. pneumophila occurs most frequently and has six serogroups (Serogroups 1-6), the first of which is the most important. Legionelloses, the diseases caused by these organisms, occur epidemically, endemically or sporadically. In clinical terms, these are acute cases of pneumonia which occur especially frequently in older persons and immunocompromised hosts. The course is severe in such patients. There is increased lethality. Erythromycin, rifampicin and cefoxitin are the most effective antibiotics. After the culture has been made in the suitable milieu and the material obtained by pulmonary aspiration has been inoculated into guinea pigs, the bacteriological diagnosis is made by direct immunofluorescence. The serological diagnosis is based on evidence of serological changes demonstrated by the indirect immunofluorescence test.

Adult↗
PubMed1982

[Bacteriologic study of 125 cases of streptococcal infectious endocarditis].

Streptococci remain the bacterial group the most frequently associated with microbial endocarditis. This paper points out the ability of the different species to cause endocarditis. 128 streptococci are isolated from patients with microbial endocarditis (127 from blood cultures, 1 from a valve). Isolates are speciated by biochemical tests and the determination of their group antigen. The viridans streptococci most frequently isolated are S. sanguis I (27 p. cent), S. mitis (16.5 p. cent) and S. sanguis II (9.6 p cent). A comparative survey of the results is rather difficult due to the methodological and taxonomical divergences.

Endocarditis, Bacterial↗
PubMed1982

[Practical problems raised by antibiotic minimal inhibitory concentration determination in Legionella species (author's transl)].

Because of culture medium constitution and growth conditions of Legionella species, antibiotic Minimal inhibitory Concentration (MIC) determination is difficult and does not fit with the accepted standards. The following strains have been tested : references strains : Staphylococcus aureus Oxford CNCM 53154, S. aureus 209P CNCM 53156, E. coli CNCM A224, E. coli CNCM 7324. L. pneumophila, 9 strains representative of the 6 serogroups. L. micdadei, L. dumoffii. 5 clinical isolates of L. pneumophila serogroup 1 (3 isolated in our Laboratory and 2 in Belgium). MIC of 17 antibiotics have been determined, among them macrolids not tested before. The method used was the Agar Dilution Method, with several media compared. MIC values are different in function of the various media tested Charcoal yeast extract medium, which is recommended for Legionella growth, does not inhibit activity of penicillin G, cefalotin, cefoxitin, amikacin, erythromycin and lincosamins, in contrast of tetracyclin, gentamicin, dibekacin, chloramphenicol, oleandomycin, pristinamycin, spiramycin and rifampicin. Charcoal, ferric pyrophosphate and L-cystein present in the culture medium are inactivating factors. However our results confirm good activity of rifampicin, erythromycin, cefoxitin, chloramphenicol, gentamicin and amikacin. Among the others macrolids tested, pristinamycin is the most active.

Anti-Bacterial Agents↗
PubMed1982

[Skin flora of the hands, elbow-hollow and fore-arm (author's transl)].

During five years, the values of the skin flora of the hands, fore-arm and elbow-hollow were studied on volunteers, especially five of them. Samples were taken according to the washing method. Results show important quantitative variations of the flora between the different volunteers and even for a same volunteer. In the five long term followed subjects, the differences were 2,17 log 10 for aerobic flora and 1,97 log 10 for aero-anaerobic flora of the hands, 3,90 log 10 for aerobic flora and 3,88 log 10 for aero-anaerobic flora of the fore-arm 2,95 log 10 for aerobic flora and 3,35 log 10 for aero-anaerobic flora of the elbow-hollow. These variations remained independent of the season. According to these variations, already notified by others authors it is suggested first to standardize sampling and bacteriological methods, second to develop multicentric programs in view to increase that number of subjects to be included in the study.

Aerobiosis↗
PubMed1981

[Tricuspid valve replacement with aortic valve allograft. Clinical and haemodynamic results more than five years after (author's transl)].

Between 1970 and 1974, twenty-three patients with advanced multivalve lesions underwent tricuspid valve replacement with a human aortic valve allograft (26-28 mm in diameter) mounted on a Shumway ring. The mean follow-up was 76.7 months. Early post-operative mortality was 4.4% and late mortality 17.4%. The actuarial survival rate was 86.6% at 5 years and 74.2% at 8 years. In 1979, 17 out of 18 survivors (one was lost sight of) were reinvestigated by cardiologists. Late functional improvement z found in 82.2% of the cases; two patients failed to improve owing to recurrent mitral stenosis. On clinical examination 12 out of 15 subjects showed no sign of right ventricular failure. In 54% the cases phonocardiography revealed abnormal tricuspid sounds indicating variable and moderate deterioration of the allograft. Haemodynamic studies showed a significant decrease in mean right atrial pressure (p less than 0.001) and mean pulmonary artery pressure (p less than 0.001), and an increase in cardiac index (p less than 0.01). The diastolic gradient through the graft was always inferior to 3 mmHg (mean 1.2 mmHg), with little change on exercise. This was significantly less than the 3.9 mmHg gradient observed with tricuspid valve replacement using Hancok valve. These were the only tricuspid valve replacements performed in our unit in 1970-74. The satisfactory long-term results obtained contrast with the frequent deterioration observed in aortic valve replacements carried out during the same period by the same team, using the same technique

Aortic Valve↗
PubMed1981

Antigenic properties and susceptibility to antibiotics of strains of Staphylococcus aureus in the 94, 96 phage complex.

Out of the 2,527 strains typed in 1976, 1977, 1978 and 1979, 226 strains (8.94%) were lysed by phages 94, 96 or 94 + 96. The two phages lyse together 83.17% of strains, whilst phage 94 alone lyses only 3.96% and phage 96 alone 12.87%. 94/96 strains have the S. aureus species antigens, particularly that of biotype A (human) at levels similar to those previously described: Protein A 96%, beta Ribitol teichoic acid (93%). Very characteristic is the presence in these strains of type antigens c1 (89%) and sigma (92%), which are significantly unusual or absent in the strains of other phages types. 94-96 complex strains are sensitive to antibiotics except resistance to Penicillin G (95%), Tetracycline (48%), Doxycycline (25%). Relation between lysis by phages 94-96 and presence of antigens c1 and sigma is discussed.

Anti-Bacterial Agents↗