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J Endo

Publications and source records attributed to J Endo.

At least 19 recordsLinked to original sources
PubMed1992

Basic evaluation of an immunoradiometric competitive inhibition assay for sialosyl-Tn antigen in sera in women. Assay conditions and normal values.

The assay conditions needed for an immunoradiometric competitive inhibition assay of sera in healthy women were studied using the monoclonal antibody TKH2, which is known to recognize specifically sialosyl-alpha 2,6-GalNAc alpha 1-0-serine/threonine (S-Tn) antigen, a mucinous cancer-related antigen. Stable results were obtained with an incubation time of 1.5 hours at room temperature. The intra-assay and inter-assay coefficients of variation were 3.27% and 3.07%, respectively. The mean (+/- standard deviation [SD]) levels of serum S-Tn in 602 healthy women was 21.2 U/ml (+/- 8.4 U/ml). Values showed a normal logarithmic distribution. Although slightly higher levels were seen in postmenopausal compared with premenopausal women, the differences were not significant. The cutoff value of 41 U/ml was determined from data obtained in 602 healthy women; higher levels were observed in only 2%. Serum S-Tn levels were not strongly influenced by Lewis or ABO (H) blood type, smoking, pregnancy, parturition, or phase of menstrual cycle. The use of the S-Tn antigen as a tumor marker for various gynecologic cancers requires study.

ABO Blood-Group System↗
PubMed1992

Use of various types of column reactors for flow-injection analysis.

Two or three different kinds of immobilized enzymes can be aligned in a minireactor so that sequential enzymatic reactions are carried out from upstream to downstream during flow-injection analysis. A lactate oxidase-catalase reactor, used as precolumn for removing pre-existing lactate in serum before the lactose dehydrogenase (LDH) reactions, was useful for the determination of serum LDH activity, which did not require any blank correction. A sequential glutamate dehydrogenase-glutamate oxidase reactor was also useful for a novel chemiluminometric determination of ammonia. On the other hand, a co-immobilized creatininase-creatinase-sarcosine oxidase reactor, in spite of containing creatininase which catalyses the reversible reaction, was the most efficient for the determination of serum creatinine.

Ammonia↗
PubMed1992

A modified technique for endoscopic mucosal resection of small early gastric carcinomas.

Endoscopic gastric mucosal resection (EGMR) is increasingly employed in early gastric cancer to remove the tumour with the surrounding mucosa and part of the submucosa after submucosal saline injection and staining to define the extent of the lesion. This study presents preliminary experience with a modified EGMR technique in five patients which allows more precise targeting and resection of the lesions. The time required for this procedure was less than 30 minutes in each case. All lesions were completely removed. In two patients, however, tumour growth invaded the submucosa and these patients therefore underwent subsequent surgery. Three patients with carcinoma confined to the mucosa were tumour-free at follow-up with repeat endoscopy and biopsy after 7 to 23 months.

Adenocarcinoma↗
PubMed1992

Potassium accelerates urinary sodium excretion during salt loading without stimulating atrial natriuretic polypeptide secretion.

1. Effects of potassium (K) supplementation (100 mEq/day) on urinary sodium (Na) excretion and on the secretion of atrial natriuretic polypeptide (ANP) during salt loading (350 mEq/day) were studied in 12 healthy salt-resistant normotensives under strictly controlled metabolic ward conditions. 2. Urinary volume and Na excretion on the first day of the high salt period (HSP) were significantly greater in the K-supplemented group (KG) than in the control group (CG). 3. There was a significant gain in bodyweight after salt loading in both groups, with a significantly greater gain in CG on the second day of HSP. Haematocrit decreased significantly during salt loading in both groups, the degree of which was significantly greater in CG. 4. Plasma norepinephrine decreased significantly during salt loading in both groups, the degree of which was significantly less in KG than in CG. A significant increase in plasma ANP was observed in CG on and after the second day of HSP, while a significant increase in plasma ANP was observed on the fifth day of HSP in KG. 5. These findings indicate that K supplementation accelerates diuresis and natriuresis, resulting in moderate suppression of volume expansion induced by salt loading and that this accelerated diuresis and natriuresis is not a result of the action of ANP.

Adult↗
PubMed1992

[Advances in automated amino acid analysis].

Recent progress in the field of amino-acid analysis, characterized by the development of fully automated chromatographic equipment, is described. For separation and quantitation of free amino acids from biological fluid, such as blood and urine, ion exchange column chromatography followed by colorimetric detection of ninhydrin reactive products is the most widely adopted methodology in the clinical field. Analytic procedures are fully automated. The computer controlled pumping mechanism guarantees a precise buffer supply for separation and peak identification and peak height or/and area detection of each amino acid in the effluent are highly reproducible. Complete analysis of more than 30 amino acids in biological fluids can be performed within 2 hours. Amino acid analysis is now easily adopted in the routine clinical laboratory and is expected to contribute to the clinical diagnosis of amino acidopathies.

Amino Acids↗
PubMed1992

[Studies on the efficient application by STKS and 8200].

We examined the usefulness of differential leukocyte counts provided by the automated STKS (Coulter) using the VCS method, the cell analyzer 8200 (Hitachi) using the image analysing as compared with that of the traditional manual method. In the STKS, about 8000 leukocytes can be counted within one minute and 5 part differential counts (neutrophils, eosinophils, monocytes, lymphocytes) are provided. The STKS may provided adequate identification of patients who have significant leukocyte abnormalities that require further study. Potential deficiencies of this system included an inability to detect abnormal lymphocytes such as ATL cells. In addition, low numbers of erythroblasts were missed, although these cells could be warned by a flagging system. In the cell analyzer 8200, the differential leukocyte count is done by the microscopic examination of stained blood films which is prepared by automatic device. Results from counts performed with this system correlated well with those obtained by the manual method. This system also missed low numbers of erythroblasts and ATL cells. Another potential deficiencies of the system is that number of cells observed within one minute were limited to about 100. When a prescribed set of cut-off limits, carefully chosen qualitatively, is introduced into these systems, automated leukocyte differential counts, with combination of STKS, 8200 and the manual method, clearly improve turn around time for laboratory blood assessments and this help to reduce unnecessary differential leukocyte counts by the manual method.

Evaluation Studies as Topic↗
PubMed1991

Blood pressure cosegregates with a microsatellite of angiotensin I converting enzyme (ACE) in F2 generation from a cross between original normotensive Wistar-Kyoto rat (WKY) and stroke-prone spontaneously hypertensive rat (SHRSP).

We investigated the linkage between high blood pressure and the ACE gene in the F2 generation between SHRSP/Izm and WKY/Izm. The male F2 rats were categorized into 3 genotypes according to a microsatellite polymorphism in the ACE gene. Significantly high blood pressure was observed in the SHRSP homozygotes when it was compared to the blood pressure of the heterozygotes. Further, after 2 or 3 months salt-loading, the blood pressure was significantly higher in the SHRSP homozygotes than in the heterozygotes and the WKY homozygotes. The heterozygotes had a blood pressure similar to that in the WKY homozygotes, indicating that the effect of the ACE gene genotype was recessive. Salt appetite was neither correlated with the salt-sensitivity nor cosegregated with the ACE genotype. The results indicate that the locus of ACE gene associates with the development of hypertension, especially salt-sensitive hypertension.

Animals↗
PubMed1991

Effects of chronic bromocriptine-induced hypoprolactinemia on plasma testosterone responses to human chorionic gonadotropin stimulation in normal men.

To study the role played by normal levels of plasma prolactin (PRL) in the secretion of testosterone (T) in the testes, we induced hypoprolactinemia with a daily dose of 5 mg bromocriptine administered orally in five normal men 20 to 35 years of age for 8 weeks. The basal PRL, T, luteinizing hormone, follicle-stimulating hormone, and maximum responses of plasma T to human chorionic gonadotropin (hCG) stimulation were measured every 2 weeks. Basal levels of plasma T were reduced in the 1st 2-week-long period of hypoprolactinemia. In the 4-week-long period of hypoprolactinemia, the maximal response of plasma T to hCG stimulation was significantly reduced. The findings suggest that normal levels of plasma PRL may play an important role in the secretion of T in the human testes in vivo.

Adult↗
PubMed1991

Activation of Na+/H+ exchange is unnecessary in the induction of c-fos mRNA in serum-stimulated vascular smooth muscle cells.

1. The effects of extracellular Na+ concentration ([Na+]o) on serum-stimulated c-fos mRNA induction in vascular smooth muscle cells (VSMC) were studied to examine whether the activation of Na+/H+ exchange and the following intracellular alkalinization are necessary in the induction of c-fos in VSMC. 2. When monitored with 2', 7'-bis (carboxylethyl)-5(6)-carboxyfluorescein, the reduction in [Na+]o in HCO(3-)-free buffer caused a dose-dependent inhibition of Na(+)-dependent intracellular pH recovery in acid-loaded VSMC. In addition, serum-stimulated intracellular alkalinization in VSMC was completely blocked by the removal of extracellular Na+. 3. By contrast, c-fos induction, quantified by slit blot hybridization of c-fos mRNA, was not fully inhibited by the reduction of [Na+]o. Furthermore, amiloride and ethylisopropyl-amiloride, inhibitors of Na+/H+ exchange, only partially blocked c-fos induction by serum. 4. We concluded from the observation above, that the intracellular alkalinization caused by the activation of Na+/H+ exchange was unnecessary in serum-stimulated induction of c-fos mRNA in VSMC.

Animals↗
PubMed1991

Genetic factors regulate the rise in blood pressure in F2 generation crossed between stroke-prone spontaneously hypertensive rats and Wistar-Kyoto rats.

1. There was no significant difference between the systolic blood pressure (SBP) of offspring derived from SHRSP mother and WKY father and the SBP of offspring derived from WKY mother and SHRSP father at the developing stage (5-13 weeks of age). 2. The degree of genetic determinations of SBP in stroke-prone spontaneously hypertensive rat (SHRSP) at 5, 7, 10 and 13 weeks of age, determined by genetic crosses between SHRSP and WKY, was 73.9, 70.8, 50.2 and 55.3% respectively. 3. Significant correlations between SBP at 5 and 7 weeks, 7 and 10 weeks, 10 and 13 weeks, also at 5 and 13 weeks of age in F2 generation crossed between SHRSP and WKY were observed. SBP falling at or above the 80th percentile group in F2 generation at 5 weeks of age were constantly higher than SBP falling at or below the 20th percentile group from 7 weeks of age onwards. 4. These results indicate that there exists 'tracking phenomenon of SBP in SHRSP' and that genetic factors regulate the rise in SBP. Tracking of SBP in F2 generation gives us new methodological insight into hypertensive mechanism in SHR.

Animals↗
PubMed1991

Genetic heterogeneity of the spontaneously hypertensive rat.

We examined DNA fingerprints of the spontaneously hypertensive rat from Shimane Institute of Health Science, Izumo, Japan, including seven substrains that were separated in the early stages of the establishment of the stroke-prone spontaneously hypertensive rat, and compared their fingerprints with those of rats from other sources. Obtained DNA fingerprints revealed that, in both the stroke-resistant spontaneously hypertensive rat and the Wistar-Kyoto rat, there is a substantial genetic difference between the rats from the National Institutes of health and from Shimane Institute of Health Science. By contrast, only a small genetic difference was observed either between the rats from the National Institutes of Health and Charles River Laboratories or among the substrains of the spontaneously hypertensive rat in the Shimane Institute of Health Science. Further, in the strains from the Shimane Institute of Health Science, there were fingerprinting bands that could distinguish either the Wistar-Kyoto rat from all the substrains of the spontaneously hypertensive rat or the stroke-prone from the stroke-resistant spontaneously hypertensive rat in spite of their close genetic backgrounds. From the observations above, we concluded 1) that there is substantial genetic variance of the spontaneously hypertensive rat between the two major sources in the world, the National Institutes of Health and the Shimane Institute of Health Science and 2) that by DNA fingerprinting analysis, it is possible to identify the restriction fragment length polymorphisms that are specific for the spontaneously hypertensive rat or the stroke-prone spontaneously hypertensive rat. These polymorphisms can be applied in the segregation study of the F2 generation.

Animals↗
PubMed1990

[Clinical epidemiology of autoimmune thyroid disorders--variation of natural history in patients with autoimmune thyroid disorders].

Clinical and epidemiological study for autoimmune thyroid disorders was performed in a rural community. Ninety-six of 1,686 subjects had asymptomatic autoimmune thyroiditis and were followed from 1979 to 1988. TRH loading test was carried out in 91 of 96 cases between 1983 and 1984. Each individual was classified into 4 subgroups. Five patients had normal levels of basal plasma TSH and had no increment of peak levels of plasma TSH (Grade G). Twenty-three had normal levels of both basal and peak plasma TSH (Grade I). The peak plasma TSH levels in 53 patients was high, but the basal was within the normal range (Grade II). Ten had high levels of both basal and peak plasma TSH (Grade III). One of the 5 Grade G cases developed overt Graves' disease in 1987 and another Grade III case suffered from goitrous Hashimoto's thyroiditis with primary hypothyroidism. Four of the ten Grade III cases and one Grade II case developed primary myxedema during the observation period. These results show that the natural history of asymptomatic autoimmune thyroiditis is variable and the prevention of overt autoimmune thyroid disorders is difficult.

Aged↗
PubMed1990

Clinical value of sialyl Tn antigen in patients with gynecologic tumors.

Sialyl Tn antigen was measured by the immunoradiometric competitive inhibition assay in sera from patients with various gynecologic tumors: 49 uterine myomas, 97 cervical cancers, 42 endometrial cancers, 63 benign ovarian tumors, and 59 ovarian cancers including eight cases of borderline malignancy. Among the patients with uterine malignancies, a few cases showed a sialyl Tn antigen level in excess of the cutoff limit (39 U/mL). In the patients with ovarian cancers, serum sialyl Tn antigen was significantly elevated: clinical stage I, 31%; stage II, 29%; and stage III, 69%. The antigen level also correlated with the effect of treatment. In addition, malignant fluid exhibited a high sialyl Tn antigen level, whereas benign fluid showed no elevation. However, serum sialyl Tn antigen was elevated in 6% of the patients with benign ovarian cysts and in 1.5% of normal volunteers. The current findings demonstrate that the lack of tumor specificity of sialyl Tn antigen limits its diagnostic value in gynecologic malignancies, but that serial measurement of this antigen appears to be useful for monitoring patients and evaluating therapy.

ABO Blood-Group System↗
PubMed1989

Cleavage action of a trypsin-like protease from Bacteroides gingivalis 381 on reduced egg-white lysozyme.

Soluble reduced lysozyme was extensively digested by a trypsin-like protease purified from the culture supernatant of the bacterium. The digestion peptides were separated and purified by reversed-phase high-performance liquid chromatography, and were subjected to amino acid analysis. The fragments were identified by their amino acid composition, and the cleavage sites in the lysozyme chain were determined. Like mammalian trypsin, the enzyme from B. gingivalis split peptide bonds non-specifically at carboxyl sides of internal arginine and lysine residues, but the lysine present at the amino terminus of the lysozyme chain was not released. In addition, the enzyme cleaved the peptide linkage at the amino side of lysine and bonds between leucine-glycine, alanine-leucine and leucine-serine. Thus the trypsin-like protease from B. gingivalis has some cleavage actions on lysozyme different from those of mammalian trypsin.

Amino Acid Sequence↗