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Biomedical subjects

J E Fulton

Publications and source records attributed to J E Fulton.

At least 55 records · Page 3Linked to original sources
PubMed1997

Cigarette smoking, weight gain, and coronary mortality: results from the Chicago Western Electric Study.

BACKGROUND: Prospective studies of overweight and coronary heart disease (CHD) have presented inconsistent findings. Previous inconsistencies may be explained by the modifying effect of cigarette smoking on the association between weight gain and coronary mortality. METHODS AND RESULTS: We prospectively studied 1531 men 40 to 59 years of age who were employed at the Hawthorne Works of the Western Electric Company in Chicago, Ill. Information collected at the initial examination in 1958 included recalled weight at age 20, present weight, height, smoking status, and other CHD risk factors. Vital status was known for all men on the 25th anniversary: 257 CHD deaths occurred over 31,644 person-years of experience. Cox regression analysis was used to investigate risk of coronary mortality associated with change in body mass index (deltaBMI) and its modification by smoking status after adjustment for age, major organ system disease, family history of CHD, and BMI at age 20. Adjustment was not performed for blood pressure or serum total cholesterol because these are intervening variables. DeltaBMI was positively associated with risk of coronary mortality in never-smokers but not in current-smokers (P for interaction =.088). For never-smokers with deltaBMI classified as stable, low gain, moderate gain, or high gain, adjusted relative risks of coronary mortality were 1.00, 1.75, 1.75, and 3.07, respectively (P for trend=.010). For current-smokers, the respective adjusted relative risks were 1.00, 0.78, 1.05, and 1.03 (P for trend=.344). CONCLUSIONS: These results support the hypothesis that cigarette smoking modifies the association between weight gain and coronary mortality. Future investigations of weight gain and coronary mortality should account for the modifying effect of cigarette smoking.

Adult↗
PubMed1997

Utilizing the ultraviolet (UV detect) camera to enhance the appearance of photodamage and other skin conditions.

BACKGROUND: Ultraviolet (UV) light (Wood's light) has been used since 1903 to document alterations in the pigment of the skin. The development of standard UV photographic methods has allowed better opportunities for patient education and the monitoring of their therapeutic progress. OBJECTIVE: To incorporate standardized UV photography into our cosmetic dermatology practice as a tool for patient teaching and monitoring therapeutic programs. METHODS: Patients with Fitzpatrick Type I to III skin types were photographed with visible and ultraviolet light. The resultant photographic prints were discussed with the patient. When appropriate, therapeutic programs to reverse the findings were instituted for these patients. Follow-up photographs were used to document changes. RESULTS: The UV camera became a useful tool in our practice. One picture was worth a thousand words. The UV camera documented pigment changes, melasma, vitiligo, and posttraumatic or postsurgical hypopigmentation. The UV camera was also useful to monitor treatment programs designed to redistribute the pigment. CONCLUSION: The UV camera should become an integral part of sun damage detection both in the physician's office and in the community. Not only does the camera document pigmentary changes but it also demonstrates improvement after therapeutic intervention.

Adolescent↗
PubMed1996

The combination of glycolic acid and hydroquinone or kojic acid for the treatment of melasma and related conditions.

BACKGROUND: Melasma continues to be a difficult problem. Although the cause is genetic, the condition is aggravated with sunlight, birth control pills, and pregnancy. Although hydroquinone is effective and has been available for years, a new product, kojic acid, has the advantage of being pharmaceutically more stable and, also, a tyrosinase inhibitor. OBJECTIVE: To evaluate on melasma and related conditions two similar formulations of glycolic acid/hydroquinone and glycolic acid/kojic acid. The therapeutic index of the two formulations is examined. METHODS: Thirty-nine patients were treated with kojic acid on one side of the face and hydroquinone in a similar vehicle on the other side of the face. The results were documented by a clinical investigator and with Wood's light examination combined with ultraviolet light photography. RESULTS: Fifty-one percent of the patients responded equally to hydroquinone and kojic acid. Twenty-eight percent had a more dramatic reduction in pigment on the kojic acid side; whereas 21% had a more dramatic improvement with the hydroquinone formulation. These results were not statistically different. The kojic acid preparation was more irritating. CONCLUSION: Both glycolic acid/kojic acid and glycolic acid/hydroquinone topical skin care products are highly effective in reducing the pigment in melasma patients. Both formulations should be available to the dermatologist to satisfy the patient's preferences.

Adult↗
PubMed1996

Cosmetic denervation of the muscles of facial expression with botulinum toxin. A dose-response study.

BACKGROUND: Botulinum toxin has been used for facial hemispasm, strabismus, and blepharospasm. Recently it has been advocated to treat the frown lines. We have extended this program to treatment of other muscles of facial expression. METHODS: Botulinum toxin is injected into the muscles of facial expression in two or three sessions to produce a temporary loss of muscle tone. A standard method of cooling, injection, and compression was developed to minimize pain and bruising. OBJECTIVE: To complete a dose-response study to document the optimum timing and amount of toxin needed for each muscle group. RESULTS: Two to five Botulinum toxin units per muscle was as adequate as higher doses. Toxin that was reconstituted 30 days earlier produced the same loss of muscle tone as freshly mixed toxin. With two or three injection sessions loss of muscle tone lasted for up to 1 year. CONCLUSION: Botulinum toxin is highly effective as an adjuvant therapy for facial rejuvenation. This minor surgical procedure can temporarily reduce the lines on the upper face and produce a pleasing effect. With proper dosing and dilution this rejuvenation program becomes cost effective.

Adult↗
PubMed1996

Dermabrasion, chemabrasion, and laserabrasion. Historical perspectives, modern dermabrasion techniques, and future trends.

BACKGROUND: Modern skin resurfacing techniques began with wire brush surgery. Chemabrasion techniques were developed combining the peel and fraise surgery. The newer CO2 lasers provide technology to also resurface the skin and provide an alternative approach to correct facial defects and facilitate facial rejuvenation. OBJECTIVE: This review article focuses on important aspects of dermabrasion, chemabrasion, and laserabrasion. An emphasis is placed on indications, preoperative skin conditioning, modern equipment, anesthesia, and postoperative dressings. RESULTS: The mainstay of therapy for skin resurfacing continues to be chemical peels. Combinations with dermabrasion are important for deeper lesions such as acne scars, or combined with laserabrasions for deeper rhagades. CONCLUSIONS: With a thorough understanding of the important principles of skin resurfacing and wound healing, the management of complexion improvement becomes straight forward, complications become manageable, and patient satisfaction is excellent.

Acne Vulgaris↗
PubMed1996

Chicken Mhc alloantiserum cross-reactivity analysis by hemagglutination and flow cytometry.

The major histocompatibility complex (Mhc) haplotype in the chicken is generally determined by the use of alloantisera in a hemagglutination assay. This method restricts haplotype determination to antigens expressed on the surface of erythrocytes which includes class I (B - F) and class IV (B - G) antigens as well as any other polymorphic molecules on these cells. Alloantisera can result in complex cross-reactivity patterns. We describe here the analysis of 53 alloantisera made within Mhc-congenic lines. Each antiserum was tested by hemagglutination with erythrocytes and by flow cytometry with erythrocytes and peripheral white blood cells of seven Mhc haplotypes; B2, B5, B12, B13, B15, B19 , and B21 . Five types of antiserum were identified based on their reactivity to different cell subpopulations of the peripheral blood of the donor haplotype as well as in cross-reactivity for different haplotypes. RBC specific cross-reactive antigens attributed to B - G molecules were demonstrated for the B5 : B19, B12 : B19, and B19 : B21 cross-reactions. Cross-reactive antigens detected on RBC and thrombocytes attributable to B - G molecules on both types of cells were demonstrated for the B2 : B12, B2 : B15, B2 : B19, and B2 : B21 cross-reactions. In addition, cross-reactive antigens occurring on RBC and WBC were attributed to B - F (or RBC and lymphocyte-expressed B - G loci) and included the B12 : B13, B13 : B19, and B15 : B19 cross-reactions. Several antisera with specificity for B cells purportedly identifying B - L epitopes were found but their numbers were limited and cross-reactivities were not defined. The identities described here may be useful in understanding B haplotype similarities and differences in disease resistance and immune response.

Animals↗
PubMed1995

Functional analysis of avian class I (BFIV) glycoproteins by epitope tagging and mutagenesis in vitro.

Similarities between the physical structures of avian and mammalian major histocompatibility complex (MHC) class I glycoproteins have been proposed based on comparative alignment of their amino acid sequences. To investigate the physical structure of the chicken class I glycoprotein, we cloned the cDNA representing the BFIV locus of the B21 haplotype. A unique, chimeric class I glycoprotein was constructed by incorporating an epitope tag (FLAG) at the N terminus. Monoclonal antibodies to the FLAG epitope served to monitor cell-surface expression for functional analysis of the BFIV21 class I glycoprotein. The chimeric class I glycoprotein was expressed in target cells using an avian leukosis virus (ALV)-derived retrovirus vector (RCASBP). The presence of the FLAG epitope did not interfere with either alloantibody recognition or cytotoxic T lymphocyte interaction. Functional analysis employing site-directed mutagenesis identified BF amino acid residues forming serologic epitopes as well as residues important in antigen presentation to ALV-induced cytotoxic T lymphocytes. BF residues 78 and 81, corresponding to HLA 79 and 82, form an antibody epitope with a slight effect on ALV antigen presentation, consistent with their predicted orientation based on the HLA-A2 crystal structure. Alignment of the BFIV21 sequence with previously published BFIV sequences revealed polymorphisms at position 34 (HLA 34), a monomorphic residues in HLA and H-2. Residue 34 is located in pocket B and is predicted to contact the main-chain carbon of peptides bound in HLA-A2. A site-directed substitution in BFIV residue 34 dramatically alters ALV antigen presentation by the BFIV21 class I glycoprotein. These data indicate that the physical molecular structure of the chicken MHC class I glycoprotein is similar to HLA.

Amino Acid Sequence↗
PubMed1995

Prediction of maximum voluntary ventilation (MVV) in African-American adolescent girls.

The purposes of the present study were to: 1) develop original equations to predict maximum voluntary ventilation (MVV) in African-American adolescent girls; and 2) determine the validity of existing MVV prediction equations for use in African-American subjects by comparing predicted MVV values in the literature with actual MVV values obtained in this study. Forced expiratory volume in 1 second (FEV1), forced vital capacity (FVC), and 12 second MVV were measured with a clinical respirometer (Spirometrics model 2451) in 93 African-American adolescent girls (13.5 +/- 1.0 years). Age (r = 0.29), height (r = 0.38), FEV1 (r = 0.52), and FVC (r = 0.48) were significantly (P < 0.01) correlated with MVV. Multiple regression analysis was used to develop equations to predict MVV with age, height, and FEV1 as predictor variables. Using Mallow's Cp criterion, age-FEV1 and FEV1 equations appeared to be the most accurate predictors of MVV. When MVV was regressed on FEV1 in a non-intercept model, the result was the commonly cited equation MVV = 35.0 x FEV1. Thus, the previously established relationship between FEV1 and MVV can be extended to African-American adolescent girls. Comparison of predicted MVV values calculated from other published equations with our subjects' actual values indicated that equations using age and/or height as predictors did not accurately predict MVV in our subjects.

Adolescent↗
PubMed1995

Silicone gel sheeting for the prevention and management of evolving hypertrophic and keloid scars.

BACKGROUND: Hypertrophic scars and keloids remain a problem for surgeons. Topical and intralesional corticosteroids, positive pressure dressings, cryotherapy, and laser therapy are helpful but not uniformly successful. OBJECTIVE: To document the effectiveness of silicone gel sheeting in the prevention and/or reduction of evolving hypertrophic scars and keloids. METHODS: Silicone gel sheeting was placed over evolving scars in 20 cases. The dressing was worn for at least 12 hours a day. Biopsies were examined for the presence of silica in the tissue. RESULTS: Lesions improved during the treatment period in 85% of the cases. The mechanisms of action are unknown. Positive pressure was not necessary. No silica from the dressing was found at the wound site. CONCLUSION: Daily treatments with silicone gel sheeting should begin as soon as an itchy red streak develops in a maturing wound. The dressing is effective in reducing the bulk of these lesions.

Adult↗
PubMed1995

In vitro analysis of a primary, major histocompatibility complex (MHC)-restricted, cytotoxic T-lymphocyte response to avian leukosis virus (ALV), using target cells expressing MHC class I cDNA inserted into a recombinant ALV vector.

The interaction between the major histocompatibility complex (MHC) and cytotoxic T lymphocytes (CTLs) is an important component of the host's resistance to viral infections and tumor formation. In this study, an avian leukosis virus (ALV) vector system, RCASBP, expressing MHC chicken class I (B-F) cDNA was used to develop target cells expressing the chicken class I glycoproteins complexed with ALV antigens on the cell surface. Peripheral blood from chickens inoculated with ALV was shown to contain antigen-specific, MHC-restricted, CD8+ effector CTLs, using a 51Cr release assay utilizing the RCASBP B-F target cells. The stimulated effector cells were also predominantly alpha beta T-cell receptor-positive (TCR2) T cells. The CTL response varied between two haplotypes of chickens which differed in their response to Rous sarcoma virus (RSV)-induced tumors. Chickens with the B21 haplotype which regress RSV-induced tumors showed maximal cytolytic activity, while chickens with the B13 haplotype which do not regress RSV-induced tumors had minimal to no cytolytic activity. In addition to assessing the CTL response to ALV, the creation of MHC-specific immortal target cell lines will be extremely useful in evaluating CTL responses to other viral disease in chickens.

Animals↗
PubMed1994

Observed levels of elementary and middle school children's physical activity during physical education classes.

BACKGROUND: The benefits of childhood physical activity include fitness, weight control, and exercise habits that may carry over into adulthood. School physical education is the primary program responsible for training the nation's youth to be physically active and national objectives call for students to be engaged in moderate-to-vigorous physical activity at least 50% of class time. The purpose of this study was to determine through systematic observation in a regional sample of elementary and middle schools the amount of moderate-to-vigorous physical activity students obtain during physical education classes. METHODS: Twenty elementary schools and 7 middle schools were randomly sampled from the 355 elementary schools and 117 middle schools in the 20 school districts in Harris County, Texas. In addition, 9 elementary and 6 middle schools that were identified as having excellent physical education programs were sampled purposively to provide a comparison. Seven systematic observations of student's physical activity were conducted during physical education in each school. RESULTS: The average moderate-to-vigorous physical activity in the randomly selected elementary schools was 8.6%, significantly less (P < 0.05) than for the randomly selected middle schools (16.1%) or for the comparison elementary schools (20.6%) and middle schools (24.0%). CONCLUSIONS: The average amount of physical activity observed in this regional sample of schools was less than the estimated national average of 27% and far less than the national recommendation calling for a minimum of 50%.

Adolescent↗
PubMed1993

Aerobic capacity in black adolescent girls.

Our purpose was to determine the aerobic capacities (VO2max) of a group of black female adolescents (age = 11.4-15.8 years) randomly chosen from a single urban school. Of 91 girls selected, 64 performed an incremental treadmill running test to volitional exhaustion and achieved VO2max as determined from expired gas measures. Other measures included height (m), weight (kg), and calf and triceps skinfolds (for % fat estimates). Girls were also asked whether they had achieved menarche. VO2max averaged 37.3 +/- 6.2 ml.kg-1 x min-1 and was significantly correlated (r[62]) with height (-.32, p < .01), body mass index (-.63, p < .001), and % fat (-.65, p < .001) but not with age (-.16, p > .10). Postmenarchal girls were significantly taller and older than premenarchal girls. Contrary to previous studies, the girls' VO2max values were not related to biological age. Our subjects' aerobic capacity values averaged 14% less than those of nonblack U.S. female adolescents previously reported in the literature. This difference in VO2max was primarily a function of body weight. Study implications support the possibility that overweight in adult black women may originate prior to or during early adolescence. Future longitudinal studies should be designed to investigate the effects of aerobic fitness on cardiovascular risk factor reduction in black adolescent girls.

Adolescent↗
PubMed1991

The prevention and management of postdermabrasion complications.

The complications of keloids, pigment changes, loss of skin texture, and enlarged facial pores remain a problem with dermabrasion patients. Their occurrence can be reduced by proper patient selection, proper dermabrasion technique, proper wound management, and prompt treatment. Keloids are rapidly resolved with the use of flurandrenolide tape covered with positive-pressure chin-strap dressings. Streaks of hyperpigmentation are lightened with a combination of sunscreen, opaque makeup, tretinoin, and hydroquinone lotions. The loss of skin texture can be prevented by not abrading too deeply and avoiding subsequent bacterial contamination during wound healing. However, enlarged skin pores in the central portion of the face and hypopigmentation can be permanent complications.

Dermabrasion↗
PubMed1990

Chicken A blood groups system antigens: molecular characteristics and lack of expression on lymphocytes.

The molecular structure of two antigens (A2 and A4) of the chicken A blood group system was determined by using an A4-specific monoclonal antibody (ISU-cA) and several alloantisera specific for chicken A blood group antigens. Molecules immunoprecipitated from erythrocytes were separated on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under either reducing or non-reducing conditions. Molecules of relative molecular weights 53.0 and 54.5 Kd were identified under reducing conditions for A2 and A4 antigens, respectively, and non-reduced molecules had a relative molecular weight of 44.5 Kd for both antigens. Two-dimensional electrophoresis showed a similar, single, diffuse band near pH 6.5 for each antigen. The data are consistent with a glycosylated molecule with one or more intrachain disulphide bonds. Allelic differences between A2 and A4 antigens seem to be due to an additional moiety on A4 antigen with a net neutral charge. Binding to chicken lymphocytes of antibody specific for A antigens was not detected by enzyme-linked immunosorbent assay (ELISA). Immunoprecipitations of radiolabelled peripheral blood lymphocyte-surface molecules using ISU-cA and A-specific alloantisera also did not detect A blood group antigens. Thus, chicken A blood group antigens are not indicated to be present on lymphocytes.

Animals↗
PubMed1990

Monoclonal antibody differentiates chicken A system alloantigens.

A monoclonal antibody (ISU-cA) was produced that recognized certain alloantigens of the chicken A blood group locus. Antigens produced by alleles A3, A4 and A8 were positive, and those produced by A2 and A5 were negative, by haemagglutination. The specificity of ISU-cA for chicken A blood group antigens was demonstrated by serologic analyses, genetic crosses and competitive inhibition of binding by anti-A alloantisera. To our knowledge, this is the first reported monoclonal antibody against a chicken blood group alloantigen system other than the B complex.

Alleles↗
PubMed1990

Ontogeny and expression of chicken A blood group antigens.

Expression of chicken red blood cell (RBC) surface antigens was studied by using a monoclonal antibody (ISU-cA) specific for chicken A blood group antigens. Erythrocytes were examined from embryos of 3-18 days of incubation and from chicks at hatch up to 21 weeks of age. Specific antigens were detected on embryonic RBC surfaces by immunofluorescence as early as 3 days of incubation. Antigenic expression was examined by both haemagglutination and immunofluorescence and found to increase with age from embryos to mature birds. The antigen concentration on the cell surface was found to be affected by genotype; heterozygotes had an intermediate level of antigen between that of the two parental genotypes. These data confirm the co-dominance that is observed with most blood group antigens. Flow cytometric analysis allowed confirmation that the entire erythrocyte population gradually increased in antigenic expression over time, rather than having an antigen-negative subpopulation being replaced by a positive subpopulation.

Animals↗