Search PubMed⌕ Search

Biomedical subjects

J Diebold

Publications and source records attributed to J Diebold.

At least 253 records · Page 14Linked to original sources
PubMed1985

Lymph node modification in patients with the acquired immunodeficiency syndrome (AIDS) or with AIDS related complex (ARC). A histological, immuno-histopathological and ultrastructural study of 45 cases.

The authors present the results of a histopathological study on the lymph-nodes taken from 45 subjects suffering from either an AIDS or from a chronic adenopathy corresponding to the definition of AIDS related complex (ARC). The various aspects observed were classed as type I to type IV. The lymph-node modifications observed in the 29 patients with an ARC could be divided into three principle groups: an extensive follicular hyperplasia associated with other elementary lesions or type IA (25 lymph-nodes from 23 patients); changes resembling a multicentric Castleman syndrome or type IB (1 case); angioimmunoblastic-like (AIL) lesions or type II (2 cases) and an association of lesions of type II (7 lymph-nodes from 6 patients). During AIDS, the adenopathy usually disappears, and the small lymph-nodes removed, especially on autopsy, show an extensive lymphoid depletion (type III) with systematic sclerosis (15 lymph-nodes from 14 patients). When adenopathy persists, it is due to infections complications (tuberculosis, cryptococcosis, avian mycobacteriosis and Whipple's disease like lesions). Of the 10 patients in whom a Kaposi's sarcoma was observed, only 6 showed lymph-node involvement, or type IV. The different histopathological lesions seem to appear according to an evolving succession, proven by certain association of lesions and by successive biopsies. In our series, 17% of subjects with an ARC evolved to AIDS. Lymph-node biopsy allows a possible ARC to be implicated on the association of the following simple lesions: follicular hyperplasia with partial or total destruction of the perifollicular lymphocytic cisterna, infiltration of the germinative centres by streams of small lymphocytes, evolving to an aspect of a "burst" germinative centre and various sinusal reactions with, in particular, the presence of neutrophilic polynuclear cells. The biopsy also allows the forms with bad prognosis to be recognized: those with AIL-like aspect or multicentric Castleman-like syndrome, which seems to represent a particular evolutive form. Finally, it also detects, in certain cases, the localization of a Kaposi syndrome, signalling the passage to AIDS. The immunopathological studies present a double interest. Firstly, they offer arguments in favour of the diagnosis: increase in the number of T8 lymphocytes in the germinative centres with the formation of small clusters and disruption of the network of dendritic reticular cells, and the inversion of the T4/T8 ratio in the extra-follicular cortical regions, by either a decrease in T4 lymphocytes or by an increase in T8 lymphocytes.(ABSTRACT TRUNCATED AT 400 WORDS)

Acquired Immunodeficiency Syndrome↗
PubMed1985

Pulmonary polymorphic centroblastic type malignant lymphoma in a patient with lymphomatoid granulomatosis, Sjögren syndrome and other manifestations of a dysimmune state.

A case of lymphomatoid granulomatosis arising in a patient with a long history of dysimmunity is reported. The autopsy reviewed the final evolution into a high grade malignant lymphoma with IgM K immunoglobulin production and discovered in deep lymph nodes modifications similar to angio-immunoblastic lymphadenopathy.

Adult↗
PubMed1985

Extended versus involved fields irradiation combined with MOPP chemotherapy in early clinical stages of Hodgkin's disease.

From 1976 to 1981, 335 patients with untreated Hodgkin's disease, clinical stages I, II, and IIIA, have been treated by MOPP (nitrogen mustard, vincristine, procarbazine, prednisone) chemotherapy, three to six cycles according to the prognostic factors, combined with radiotherapy. Irradiation was always performed after the first three cycles of chemotherapy, and was randomized between extensive radiotherapy, ie, mantle and paraaortic areas for supradiaphragmatic presentations, and radiotherapy restricted to the involved areas. No significant difference was observed between the two randomized branches for the disease-free survival (86% after six years in the involved field branch v 90% in the extended field branch), and none for the overall survival. Most of the relapses occurred in nonirradiated areas in the first group, and in irradiated areas in the second. Relapses were especially frequent in the IIE stages with pulmonary extension; extranodal relapses occurred with osseous and cutaneous localizations. Two cases of secondary leukemia were observed after three- or six-cycle MOPP plus radiotherapy limited to the involved areas.

Adolescent↗
PubMed1985

Human follicular dendritic cells express CR1, CR2, and CR3 complement receptor antigens.

The expression of complement receptors by human follicular dendritic cells (FDC) was investigated by immunohistochemical techniques by using polyclonal and monoclonal antibodies to antigenic determinants of CR1, CR2, and CR3. Upon optical immunohistochemical examination of frozen sections from human reactive lymph nodes and tonsils by a three-step immunoperoxidase technique, a strong staining of cell bodies and cytoplasmic extensions of FDC was observed in germinal centers with anti-CR1 and anti-CR2 antibodies. Staining for these antigens was also found on cytoplasmic extensions of FDC in the mantle zone and on the plasma membrane of B cells in the entire follicles. Staining of FDC with anti-CR2 antibody was more intense than that of B lymphocytes. Monoclonal antibodies directed against epitopes of the alpha-chain of CR3 weakly stained FDC in follicles in a similar pattern to that which was observed on adjacent sections with mouse monoclonal antibody KIM4 that only recognizes FDC in human lymph nodes. Immunoelectron-microscopy was performed on frozen sections of a lymph node involved with a centroblastic centrocytic B malignant lymphoma and a reactive tonsil with the use of rabbit F(ab')2 anti-CR1 antibodies and mouse monoclonal anti-CR2 antibody. All the plasma membrane of the cell body and cytoplasmic extensions of FDC in germinal centers and in the mantle zones homogeneously stained for CR1 and CR2 antigens. Fibroblastic reticulum cells were negative. The plasma membrane of tumoral B lymphocytes strongly stained with anti-CR1 and weakly stained with anti-CR2 antibodies. The presence of CR1, CR2, and CR3 on FDC is a unique surface characteristic of these cells that should optimally allow the cells to bind antigen/antibody complexes bearing any type of C3 fragment.

Antibodies, Monoclonal↗
PubMed1985

[Viral type particles in the germinal centers during a lymphadenopathic syndrome related to AIDS].

The identification of virus-like particles in the germinal centers of an AIDS-related lymph node with histological findings of group IA is reported. A 23 year-old man, intravenous drug user, presents an inversion of T helper/T suppressor cell ratio and a peripheral generalized lymphadenopathy, suggesting a diagnosis of ARC. Lymph node biopsy shows follicular hyperplasia and features consistent with group IA of AIDS-related lymphadenopathy. Immunohistochemical study reveals an increase of intrafollicular T8+ lymphocytes. Electronmicroscopic examination of follicles demonstrates the presence of particles showing features of viral nucleocapsids; these particles are seen between the cytoplasmic processes of follicular dendritic cells. Pathologic findings associated with lymphoid follicular hyperplasia in AIDS prodromal period are examined. The importance of lymph node biopsy with histological, immunohistochemical and ultrastructural studies, in patients at high risk for AIDS, is demonstrated. The detection of type D viral particles suggestive of retrovirus is in accordance with results of virologic studies which indicate the involvement of a retrovirus (called LAV or HTLV III) in the etiology of the disease.

Acquired Immunodeficiency Syndrome↗
PubMed1985

[Bronchial localization of a T-cell immunoblastic lymphoma. Transformation from mycosis fungoides].

A case report is presented of a patient with a pleural effusion subsequently shown to have a localised immunoblastic lymphoma of the bronchus of non-Hodgkin's type. The development of this lymphoma in a patient with mycosis fungoides underlines the potential for the transformation of a T cell epidermoid lymphoma of low grade malignancy to a lymphoma of high grade malignancy.

Bronchial Neoplasms↗
PubMed1985

[Cervical adenopathies in relation to AIDS. Course of histological aspects. Diagnostic and prognostic value].

Histopathologic appearances of lymph nodes during acquired immune deficiency syndrome (AIDS) and related conditions are analyzed, with emphasis on the diagnostic and prognostic value of cervical gland biopsy at the lymphadenopathic stage. Details are given of the characteristics of the follicular hyperplasia of the lymph nodes (type I), which appears to be the initial lesion and is possibly regressive, and emphasis placed on the poor prognostic significance of the hyperplasia simulating a Castleman syndrome and of the forms simulating an angioimmunoblastic lymphadenopathy (type II). Findings suggest that the appearance, in a still hyperplastic lymph nodes, of foci of pseudo-angioimmunoblastic homogenization, of fibroedema with lymphoid depletion or conjunctival vascular proliferation may be the initial sign of a course towards the glandular atrophy (type III) or Kaposi's sarcoma which are the attribute of confirmed AIDS.

Acquired Immunodeficiency Syndrome↗
PubMed1984

Immunoelectronmicroscopic profile of intracytoplasmic immunoglobulins in B non Hodgkin's malignant lymphomas. Comparison with the normal pattern of B lymphoid cells.

An immunoelectronmicroscopic method using Fab fragment of anti human IgG (H + L) has been employed to study the localization of cytoplasmic immunoglobulins in the tumoral cells of 12 B non Hodgkin's malignant lymphomas (B-M.L.). A comparison with normal homologous B lymphoid cells was performed on 10 non tumoral reactive lymph nodes. Immunostaining was observed in PNC, RER and Golgi complex. The criterions of differentiation were discussed in the different B-M.L.. Because of a granular hyaloplasmic immunostaining in normal and tumoral centroblasts and immunoblasts, monospecific antibodies against gamma, mu, alpha heavy chains were used to rule out a non specific uptake. Presence of mu heavy chain was discussed as an argument for immunoglobulin free ribosomal synthesis.

B-Lymphocytes↗
PubMed1984

[Malignant centroblastic and immunoblastic lymphoma. Results of a direct immunofluorescence study of 43 samples after inclusion in paraffin].

The immunosecreting character of tumour cells was shown using a direct immunofluorescent technique on histological sections of fixed tissue, included in paraffin, of 43 specimens from 39 patients suffering from highly malignant ML. These ML corresponded, in the Lennert-Kiel classification, to immunoblastic (9 cases, 4 in plasmoblastic transformation) or polymorphic centroblastic ML (34 cases). In seven patients, the highly malignant ML was secondary to a low grade malignancy ML (3 CLL; 1 lymphoplasmocytic ML; 1 centroblastic centrocytic follicular ML) or to previous dysimmune state (1 Gougerot-Sjögren syndrome 1 lymphomatoid granulomatosis of Liebow). Of these 43 specimens from diverse tissues, but predominantly lymphatic lymph nodes (22 cases), 14 did not contain any monoclonal intracytoplasmic immunoglobulin (12 polymorphic centroblastic ML; 2 immunoblastic ML). On the other hand, 8 immunoblastic ML and 21 polymorphic centroblastic ML did secrete a monoclonal immunoglobulin. Among the 29 cases, 23 contained a heavy chain associated with only one light chain. In one case of Richter's syndrome, a double population, one mu, the other gamma, associated to only the light chain Kappa, was demonstrated. The heavy chain mu was secreted in 25 cases out of 29, associated 19 times to a Kappa chain and 4 times to a Lambda chain. The authors stress the morphological aspects of the positive fluorescent reactions, which plead in favour of a real secretion by the cells: perinuclear border with small grains projecting into the nucleus; intranuclear vacuoles; positive cytoplasmic reactions with more or less confluent granules, predominantly perinuclear.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1984

[Histopathologic aspects of bone marrow in angioimmunoblastic lymphadenopathy].

42 bone marrow biopsies (BMB) were done in 34 patients with angio-immunoblastic lymphadenopathy (AIL), 3 of them associated with Gougerot-Sjögren syndrome. Involvement of bone marrow was discovered in 36 BMB (85%) and in 27 patients (79%). The lesions appeared as nodules destroying the hematopoietic tissue. Each nodule was constituted by a variable amount of lymphocytes, plasma cells, immunoblasts and histiocytes. Epithelioid cells were associated in 14 BMB and in 11 patients. Collagenous myelofibrosis was seen in and around nodules. The bone marrow lesions were uninodular in 5 BMB (11.9%), multinodular in 17 patients and 20 BMB (47.6%) or massive in 10 patients and 11 BMB (26.1%). Vascular lesions were disclosed in only 17 BMB with multinodular or massive involvement. The hematopoietic bone marrow outside the nodular lesions showed hyperplasia in 12 BMB and was normal in all other cases. In the 7 BMB without AIL localization the hematopoiesis was 6 times normal and hyperplasic in one case. No case of malignant lymphoma was disclosed. AIL bone marrow involvement should not be confused with non Hodgkin's ML or Hodgkin's disease. Correlation with median survival and bone marrow involvement was shown: 12 months in cases with massive involvement, 16 months in uni or multinodular lesions, 56 months in bone marrow free of disease. So BMB is useful for the diagnosis of AIL and the presence or absence of bone marrow involvement is of prognostic value.

Bone Marrow↗
PubMed1984

[Anatomopathological aspects of non-Hodgkin's malignant rectocolic lymphoma].

The malignant lymphomas (ML) of the colon are the rarest of the non-hodgkinsian ML of the digestive tract. The caecum and rectum are the most frequent locations for these colonic ML. They can occur as primary tumours or as secondary manifestations resulting from the evolution of other primary ML in other locations. The macroscopic appearance varies according to the size and number of lesions. The histological classifications in current use are based on both morphological and functional data. The most interesting of these is the one proposed by Lennert which distinguishes between the ML of low malignancy (tumours where small cells predominate) and those of high malignant (tumours made up of large cells). The prognostic depends on both the stage of growth and the type of treatment applied.

Adolescent↗
PubMed1984

[Lymph node localization of epidermotropic T malignant lymphomas (mycosis fungoides and Sézary's syndrome)].

In this paper are reported the different histological variants of Mycosis Fungoïdes (M.F.) observed in 48 lymph node positive biopsies providing from 98 lymph nodes obtained from 94 patients with M.F. A partial destruction of the lymphoïd architecture was required for attesting M.F. involvement. The remaining 50 lymph nodes demonstrated dermopathic non specific lesions. A partial lymph node involvement is often observed with a persistent sinusal pattern and residual germinative follicles (39 from 48 cases or 80%). The tumoral infiltration begins in the deep cortex and in the interfollicular areas. Some reactive lesions of dermopathic lymphadenopathy are frequently associated. The main histological feature is the cellular pleomorphism. Nevertheless 4 cytological variants may be described according to the predominant proliferating cells: a small cell variant, a large cell variant, a mixed cell variant comprising an equivalent proportion of small medium and large-sized cells, an immunoblastic variant with majority of immunoblasts and some Sternberg-like tumoral cells. Any form may be associated with granuloma which increase polymorphism. In some histological variants, so-called Lutzner cells are numerous and facilitate the diagnosis of epidermotropic malignant T lymphoma. Nevertheless, some cases may be discussed with centrocytic or centroblastic centrocytic lymphomas, with Pinkus multilobulated T lymphoma or with convoluted lymphoblastic lymphoma. More difficult is the distinction from Lennert T - zone lymphomas and from adult T-cell lymphoma/leukemia described in Japan. The immunoblastic variant may be mistaken with some high-grade malignant non-Hodgkin lymphoma, with an angio-immunoblastic lymphadenopathy, even with a metastatic carcinoma. The evidence of granuloma with numerous eosinophiles frequently observed in the Hodgkin-like variant is a cause of confusion with Hodgkin's disease. A good help would be supplied by the discover of some Lutzner cells among the granuloma. No correlation was found between these histological variants and the M.F. evolution. Evidence of reactive inflammatory cells do not interfere with the prognosis.

Biopsy↗