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Biomedical subjects

J D Oriel

Publications and source records attributed to J D Oriel.

At least 91 records · Page 5Linked to original sources
PubMed1977

Interrelationship of Chlamydia trachomatis and other pathogens in the female genital tract.

The isolation of Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, and Candida albicans in the female genital tract was studied in 1323 patients attending a venereal disease clinic. Disruption of the cell monolayers use for the isolation of C. trachomatis was significantly associated with the presence of T. vaginalis; this effect was markedly reduced by the addition of vancomycin to gentamicin and amphotericin B in the transport and growth media. The only significant positive association was the more frequent isolation of C. trachomatis in the presence of N. gonorrhoeae. There was a negative association between N. gonorrhoeae and C. albicans and between T. vaginalis and C. albicans, the fungus being isolated significantly less frequently when these microorganisms were present.

Candida albicans↗
PubMed1977

Spectinomycin hydrochloride in the treatment of gonorrhoea: Its effect on associated Chlamydia trachomatis infections.

Sixty-three heterosexual men were successfully treated with a single injection of spectinomycin hydrochloride 2 g for urethral infections with Neisseria gonorrhoeae. Chlamydia trachomatis was recovered from the urethra of 11 of these men both before and after treatment. In six men, the organism was isolated after but not before treatment. No isolates were obtained from the remaining men either before or after treatment. All 17 of the men who yielded C. trachomatis developed post-gonococcal urethritis. Eight of 46 men from whom no isolate was obtained in their cultures developed post-gonococcal urethritis. Seventeen of 50 women successfully treated with spectinomycin for cervical infections with N. gonorrhoeae yielded isolates of C. trachomatis both before and after treatment. The organism was isolated from five women before but not after treatment, and from four women after but not before treatment. In 24 women culture for C. trachomatis was negative both before and after treatment. Spectinomycin hydrochloride in the dosage used rarely eliminated C. trachomatis from the genital tract of either men or women; in this respect it resembled two other drugs commonly used for the treatment of gonorrhoea-pencillin and ampicillin.

Chlamydia Infections↗
PubMed1976

The lack of effect of ampicillin plus probenecid given for genital infections with Neisseria gonorrhoeae on associated infections with Chlamydia trachomatis.

Forty-six men were successfully treated with a single oral dose of ampicillin (2 g) plus probenecid (1 g) for urethral infections with Neisseria gonorrhoeae. Chlamydia trachomatis was isolated from cultures of cells obtained from 11 of these men both before and after treatment; C. trachomatis was isolated from one man before but not after treatment and from three men after but not before treatment. No isolates were obtained from the remaining 31 men either before or after treatment. Of the 15 patients whose cultures yielded C. trachomatis, 12 developed postgonococcal urethritis; of the 31 patients from whose cultures no isolate was obtained, five developed postgonococcal urethritis. Of 44 women successfully treated with ampicillin plus probenecid for cervical infections with N. gonorrhoeae, 18 had C. trachomatis isolated from the cervix both before and after treatment. C. trachomatis was isolated from five women before but not after treatment and from two women after but not before treatment. No isolates were obtained from the remaining 19 women either before or after treatment. Thus ampicillin plus probencide in the dosage used rarely eliminated C. trachomatis from the genital tract of either men or women. Whereas men with a persisting chlamydial infection will probably develop postgonococcal urethritis and thus receive appropriate treatment, such an infection in women is not likely to be suspected unless attempts are made to isolate C. trachomatis.

Ampicillin↗
PubMed1976

Simplified serological test for antibodies to Chlamydia trachomatis.

Three-hundred sixty sera from unselected patients attending two London venereal disease clinics were examined by a microimmunofluorescence test. Eleven egg-grown serotypes of Chlamydia trachomatis and the so-called "fast" strain SA2(f) were used as antigens. Of the 360 sera tested, 119 (33%) reacted to a titer of 1:16 or above with at least one antigen. Of these positive sera, over 50% cross-reacted with all 12 serotypes, and 95.5% reacted with SA2(f) in addition to other antigenic types. It is suggested that SA2(f) could be used as a single antigen for preliminary screening of a large number of sera for the presence or absence of chlamydial antibody.

Antibodies, Bacterial↗
PubMed1976

Chlamydial infection of the male urethra.

Urethral specimens from 477 men were collected with endourethral swabs and examined for Chlamydia trachomatis by cell culture on McCoy cells pretreated with idoxuridine. Of these men, 141 had gonococcal urethritis, 262 non-gonococcal urethritis (NGU), and 74 showed no evidence of urethritis. Of 118 men with heterosexually acquired gonococcal urethritis, thirty (25 per cent.), and of 23 men with homosexually acquired gonococcal urethritis, five (22 per cent.), yielded C. trachomatis from the urethra. Urethral specimens from 240 heterosexual men with NGU were examined, and 118 (49 per cent.) yielded C. trachomatis. Of these 240 men, 140 gave a past history of gonococcal or nongonococcal urethritis and 67 (48 per cent.) of these were positive for C. trachomatis; no past history was given by 100 men, of whom 51 were positive for C. trachomatis. Of the 240 heterosexual men with NGU, 81 had had symptoms for 7 days or more before examination, of whom 48 (59 per cent.) yielded isolates of C. trachomatis, and 145 had had symptoms for less than 7 days, of whom 59 (41 per cent.) yielded isolates. Of fourteen asymptomatic men, three were positive for C. trachomatis. Of 22 homosexual men with NGU, seven (32 per cent.) yielded C. trachomatis. C. trachomatis was recovered from the urethra in three (5 per cent.) of sixty heterosexual men without urethritis, and none of fourteen homosexual men without urethritis yielded C. trachomatis.

Adult↗
PubMed1975

Infection with Chlamydia group A in men with urethritis due to Neisseria gonorrhoeae.

Men with urethritis due to Neisseria gonorrhoeae were treated with gentamicin, which is inactive against Chlamydia. Urethral specimens were collected before treatment and one and two weeks after treatment and cultured for Chlamydia in irradiated McCoy cells. The overall incidence of chlamydial infection was 15 of 44 (34%). All of 15 Chlamydia-positive men and 11 of 29 Chlamydia-negative men (38%) developed postogonococcal urethritis two weeks after treatment. Pre-treatment sera were examined by a complement fixation test and a simplified indirect fluorescent antibody (FA) test with a yolk sac-cultured antigen, strain SA2)f). Sera from all of 15 Chlamydia-positive men had titers of larger than or equal to 1:16 in the indirect FA test; 13 of 29 Chlamydia-negative men (45%) had positive tests. The complement fixation test was insensitive, detecting chlamydial antibodies in only one of 15 sera from Chlamydia-positive men. These results suggest that Chlamydia may cause many cases of postgonococcal urethritis.

Antibodies↗
PubMed1975

Laboratory procedures for the isolation of chlamydia trachomatis from the human genital tract.

The technique of isolating Chlamydia trachomatis from the human gential tract by centrifuging clinical specimens on to cell monolayers with subsequent incubation has been improved and simplified. Gentamicin in the media was found to be superior to streptomycin in reducing bacterial contamination of specimens. The infectivity of chlamydial suspensions of laboratory cultured material was significantly reduced by storage at +4 degrees C for more than 48 hours, and by immediate freezing to -70 degrees C. When compared with immediate processing of the specimens, freezing to -70 degrees C was found to reduce the isolation rate of C.trachomatis from men with non-gonococcal urethritis (NGU) by approximately 20%. McCoy cells pretreated with idoxuridine were compared with irradiated McCoy cells for the isolation of C. trachomatis from clinical specimens. There was no significant difference in sensitivity between the two systems, but the former is considerably simpler. The effect of the centrifugal force used for inoculating specimens on to the cell monolayers on the isolation rate of C. trachomatis was studied in groups of men with NGU. Maximal isolation rates were obtained with forces of about 3000 G, which were not significantly raised by further increasing the force used. It is suggested that the isolation of C. trachomatis from the genital tract is now well within the capacity of any laboratory equipped with simple cell culture facilities.

Cells, Cultured↗
PubMed1975

Effects of minocycline and tetracycline on the vaginal yeast flora.

Two groups of women with non-specific genital infection were treated for two weeks with tetracycline 250 mg six-hourly and minocycline 100 mg twice daily respectively. Cultures for yeasts were performed before treatment and at the end of the first and second weeks. Before treatment yeasts, mostly Candida albicans, were recovered from 13% of the women. After one and two weeks' treatment yeasts were isolated from 22 and 29% of women treated with tetracycline, and from 19 and 29% of women treated with minocycline. It is concluded that despite inhibition of the growth of yeasts shown by minocycline in vitro, there is no evidence of any significant difference between the actions of tetracycline and minocycline on the vaginal yeast flora.

Candida albicans↗
PubMed1975

Minocycline in the treatment of nongonococcal urethritis: its effect on Chlamydia trachomatis.

The effect of minocycline on nongonococcal urethritis (NGU) was investigated, with particular reference to its action against Chlamydia trachomatis. Preliminary laboratory studies showed that the drug was active against a laboratory chlamydial strain (Lb4f) in vitro. A group of 133 men with first attacks of NGU was then treated with minocycline, 100 mg twice daily for 3 weeks. Isolates of C trachomatis were obtained from 33 of these men before treatment; all of them gave negative results on cell culture after therapy. Of the 133 patients, 119 (90%) had symptoms on their first attendance, but after one week's treatment only 16 of the 133 (12%) and after 3 weeks 6 of the 133 (5%) had symptoms. All 133 men had demonstrable urethritis initially; by the end of the first week of treatment only 43 of the 133 (32%) and by the end of the third week 32 of the 133 (24%) had evidence of urethritis. There were no differences in clinical response between the Chlamydia-positive and Chlamydia-negative groups. A group of 24 female sexual contacts of men with NGU yielded C trachomatis on cell culture. After 3 weeks' therapy with minocycline all these women gave negative results on cell culture. It is concluded that minocycline is a useful addition to existing remedies for NGU. The future structure of treatment trials for NGU is discussed.

Animals↗