Biomedical subjects

J Czernicki

Publications and source records attributed to J Czernicki.

44 records · Page 3Linked to original sources

PubMedDate not supplied

[Activity of various lysosomal hydrolases in peripheral blood granulocytes in patients with multiple sclerosis].

The activity of acid phosphatase, acid and neutral proteases in the serum, white blood cells and lysosomal granules and in the supernatant of the lysosomal fraction of peripheral blood granulocytes was determined in 33 patients with multiple sclerosis. The control group included 20 clinically unaffected subjects. A rise in the activity of neutral proteases in white blood cells was demonstrated at the time of multiple sclerosis exacerbations and a fall in the activity of these enzymes in lysosomal granules with its simultaneous rise in the supernatant of the lysosomal fraction of granulocytes was observed.

Acid Phosphatase↗

PubMedDate not supplied

[Suppressor cell activity in multiple sclerosis and other nervous system diseases. Preliminary report].

3H-Thymidine incorporation was assessed in concanavalin A-stimulated cultures of peripheral blood lymphocytes of healthy subjects and multiple sclerosis patients. At the same time the counts of T and B lymphocytes were determined in peripheral blood and the activity of suppressor cells was determined in vitro by the method of Shou et all. in these patients and in other neurological diseases. The studied immunological parameters showed some variability in patients with multiple sclerosis: at the time of exacerbations the activity of suppressor cells decreased and the mitogenic response to Con A was impaired. In remissions and in slowly progressive cases of multiple sclerosis the activity of suppressor cells was increased, particularly in elderly patients. The reported investigations demonstrated that disturbances in the functions of suppressor cells occur also in other neurological diseases. The role of the observed disturbances in the pathogenesis of multiple sclerosis is discussed.

Adult↗

PubMedDate not supplied

Suppressor cells function in healthy persons.

The investigations were performed on lymphocytes isolated from peripheral blood of 26 healthy persons aged from 20 to 68 years. The following parameters were evaluated: T suppressor cell activity in two-step culture, concanavalin A (Con A) responsiveness in three-day culture and number of T lymphocytes carrying receptor for Fc portion of immunoglobulin G (TG cells). A slight increase in T suppressor activity and diminished responsiveness to Con A have been shown to be age-dependent. A correlation has been found between suppressor cells activity in vitro and the percentage of TG cells (r = 0.62, p less than 0.05). The level of suppressor activity of lymphocytes was independent of their Con A responsiveness.

Adult↗

PubMedDate not supplied

[In vitro cytotoxic activity of peripheral blood lymphocytes in patients with multiple sclerosis. Preliminary report].

Using as target cells chicken erythrocytes labelled with chromium 51Cr the cytotoxic activity (spontaneous and induced with PHA and Con A mitogens) of peripheral blood lymphocytes was studied in patients with multiple sclerosis. The control group comprised healthy subjects, patients with systemic lupus erythematosus and other central nervous system diseases. No changes were observed in the cytotoxic activity of lymphocytes of patients with multiple sclerosis without respect to the course and a duration of the disease process. On the other hand, in patients with SLE a decrease of cytotoxic activity was observed in vitro, both spontaneous activity (p less than 0.01) and mitogen-induced (p less than 0.05) as compared with other group of patients.

Adult↗

PubMedDate not supplied

[Mitogenic activity in vitro of lysosomal proteins of peripheral blood granulocytes in patients with multiple sclerosis].

Incorporation of 3H thymidine was investigated in cultures of lymphocytes obtained from peripheral blood of 33 multiple sclerosis patients and 20 healthy controls in presence of PHA or lysosomal proteins from granulocytes. The number of T lymphocytes was determined also in the peripheral blood of these patients. A rise was observed in the mitogenic activity of proteins obtained from lysosomal granules of granulocytes of multiple sclerosis patients at the time of disease exacerbation with a fall of this activity at the time of remission. At the same time a fall of T-lymphocyte count was observed in the blood of multiple sclerosis patients. The correlations between 3H-thymidine incorporation into lymphocytes in vitro, and the mitogenic activity of proteins obtained from lysosomal granules of granulocytes and the count of T lymphocytes are discussed.

Acute Disease↗

PubMedDate not supplied

[Effect of laser biostimulation on leg blood flow in the course of arteriosclerosis].

Blood flow pattern (maximum moment velocity, mean velocity, artery diameter and minute flow) in the superficial femoral artery and in the foot artery was evaluated in 30 patients, subjected to laser biostimulation for symptoms of atherosclerotic ischemia of the lower limbs. The blood flow examination was performed before the treatment and immediately afterwards (20 procedures) and 6 months following the therapy. It was found that laser biostimulation had a favorable effect on the blood flow pattern, particularly in the initial stage of ischemia (Io), and may be a useful method of medical treatment in atherosclerotic ischemia of the lower limbs.

Adult↗

PubMedDate not supplied

Activity of adenosine deaminase in red blood cells of patients suffering from multiple sclerosis treated with adrenocorticotropic hormone.

Adenosine deaminase (ADA) activity was studied in red blood cells of patients suffering from multiple sclerosis treated with adrenocorticotropic hormone (ACTH). ADA activity in hemolysates was determined according to the method of Hopkinson and calculated as units per g of hemoglobin. Activity of adenosine deaminase in healthy subjects was 0.871 +/- 0.251 U/g Hb. In patients with multiple sclerosis, before treatment ADA activity was 0.765 +/- 0.131 U/g Hb and was about 15.2% lower than in the control group (p < 0.02). After treatment with ACTH, ADA activity increased to 1.005 +/- 0.211 U/g Hb (p < 0.001). We have suggested that increased activity of adenosine deaminase in red blood cells of patients suffering from multiple sclerosis after treatment with ACTH is caused by diminution of superoxide generation, and therefore its sparing effect on cell membrane and enzyme is connected with membranes.

Adenosine Deaminase↗

PubMedDate not supplied

Red blood cell superoxide dismutase and catalase activities in patients suffering from multiple sclerosis treated with adrenocorticotropic hormone.

The purpose of this study was to determine superoxide dismutase (SOD) and catalase (CAT) activities in erythrocytes of patients with multiple sclerosis treated with ACTH. SOD activity in hemolysates was determined according to the method of Misra and Fridovich and calculated as units per gram of hemoglobin (Hb). CAT activity in hemolysates was determined with Beers and Sizer's method and expressed in IU/g Hb. SOD activity in control group was (1.61 +/- 0.45) x 10(3) U/g Hb whereas, the activity of CAT amounted to (5.88 +/- 1.36) x 10(4) U/g Hb. Before the treatment, SOD activity was decreased by approximately 20% ((1.25 +/- 0.25) x 10(3) U/g Hb) while that of CAT-by about 7.7% ((5.43 +/- 0.68) x 10(4) U/g Hb) in comparison to the normal control. After treatment with ACTH, activity of both enzymes increased: SOD-by about 34.4% to (1.68 +/- 0.38) x 10(3) U/g Hb and CAT-by about 7% to (6.29 +/- 0.55) x 10(4) U/g Hb. Results of investigations showed that ACTH caused an increase in CAT and SOD activities in erythrocytes of patients after three-week treatment.

Adrenocorticotropic Hormone↗