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Biomedical subjects

J Czernicki

Publications and source records attributed to J Czernicki.

At least 37 records · Page 2Linked to original sources
PubMed1982

Some T-cell functions in patients with viral myocarditis. The effect of levamisole.

Some T-cell functions in vitro have been studied in 16 healthy persons, 8 patients with past myocarditis, without any circulatory after-effects and in 12 patients with chronic viral myocarditis. Decrease of T-suppressor cell activity and enhanced blastic transformation of lymphocytes induced with allogeneic heart antigens have been observed in patients suffering from myocarditis. In six patients with active inflammatory process of the heart muscle, application of levamisole brought about not only clinical and electrocardiographic improvement but also an increase of suppressor cells activity and diminished blastic transformation induced with the allogeneic heart antigens and a rise of the T-lymphocytes percentage.

Adult↗
PubMed1981

The effect of lysosome factors derived from PMNL of multiple sclerosis and systemic lupus erythematosus patients on suppressor cell activity in vitro.

The lysosomal factors obtained from PMNL of healthy persons and patients with multiple sclerosis, systemic lupus erythematosus and other neurological diseases inhibited in vitro the generation of Con A-induced suppressor cell activity. The results obtained proved that these factors from granulocytes of MS-relapse and active LE have a stronger inhibitory effect on suppressor cell activity than on healthy ones.

Adult↗
PubMed1981

The effect of polymorphonuclear leukocyte (PMNL) factors on T suppressor cell function in in vitro studies in healthy persons and some autoimmune disorders.

Lysosome proteins derived from peripheral blood granulocytes of patients with multiple sclerosis (MS), lupus erythematosus (LE), rheumatoid arthritis (RA) and recurrent uveitis cause the impairment of Con A-induced suppressor cell activity in two-step culture in vitro. This effect is independent of lysosome protease activity. Such a phenomenon, observed in vitro, may cause disturbances in suppressor cell function in the course of the above diseases.

Adult↗
PubMed1979

Influence of ACTH on activity of some granulocytic proteases in patients with multiple sclerosis.

The effect of treatment with ACTH on activity of granulocytic acid and neutral proteases in patients suffering from multiple sclerosis was studied. Protease activity was distinctly higher in granulocytes from patients. ACTH normalized the activities investigated in patients with exacerbating course multiple sclerosis, but had no influence on hydrolases in the progressive form of the disease.

Adolescent↗
PubMed1976

Polymorphonuclear leukocyte lysosome activities and lymphocyte transformation in multiple sclerosis and some other central nervous system chronic diseases.

The role of PMNL lysosomes was studied in some lesions of the central nervous system with speical regard to multiple sclerosis patients. The significant decrease of acid hydrolases was observed in multiple sclerosis (MS) peripheral blood PMNL lysosomal granula and, in parallel, the increase of lysosome proteins mitogenic potency. The other lesions of the central nervous system such as apoplexy, brain tumors, brain trauma and epilepsy have not shown the MS-like activities of PMNLs. The role of PMNLs in pathogenesis of MS and in the stimulation of immunocompetent cells is suggested.

Adult↗
PubMedDate not supplied

[Evaluation of certain leukocyte subpopulations in the peripheral blood and cerebrospinal fluid in multiple sclerosis].

Using a mixed rosette technique the counts of lymphocytes forming rosettes E and EA and non-rosette-forming lymphocytes were determined in the peripheral blood and cerebrospinal fluid in 15 multiple sclerosis patients and 17 controls. A rise was observed in the percent and absolute value of non-rosette-forming lymphocytes in the peripheral blood and cerebrospinal fluid in these patients. Various immunological aspects of this fact in the pathogenesis of multiple sclerosis are discussed.

Adult↗
PubMedDate not supplied

[A case of Guillain-Barré syndrome].

The authors describe a case of Guillain-Barré syndrome with a high protein level in the cerebrospinal fluid. During the treatment corticotherapy was found to be of low effectiveness but a gradual improvement followed a combined treatment with imuran and corticosteroids with simultaneous intrathecal hydrocortisone administration despite persistence of changes in the concentration of total protein and immunoglobulins in the cerebrospinal fluid.

Adult↗
PubMedDate not supplied

[T-lymphocytes with a receptor for the immunoglobulin Fc fragment in the blood of multiple sclerosis patients].

In 12 multiple sclerosis patients and 15 healthy subjects the suppressor activity in vitro by the method of Shou et al. was determined parallelly with the number of T-lymphocytes with the receptor for the Fc fragment of immunoglobulin G (TG lymphocytes). In healthy subjects a correlation (p less than 0.05) was demonstrated between the suppressor activity of lymphocytes in vitro and the number of TG lymphocytes in the peripheral blood. On the other hand, in multiple sclerosis patients a decrease was observed frequently in the suppressor activity of these cells in vitro (especially during exacerbation of the disease) while the number of TG lymphocytes in the peripheral blood was normal or raised sometimes. Different causes of the this phenomenon in multiple sclerosis are discussed.

Adult↗
PubMedDate not supplied

Participation of granulocytes in in vitro regulation of suppressor cell activity.

In in vitro investigations factors were found in the specific granules of peripheral blood granulocytes of healthy subjects, which inhibited the induction of non-specific suppressor cells (S cells) in doses of 10 and 25 micrograms per 1 ml (1 X 10(6) cells) of 48-hour culture. In two-stage culture the S cells decreased the proliferation of responder lymphocytes (R cells) stimulated with Con A and pokeweed mitogen (PWM) by 56.8 +/- 2.4 per cent and 19.7 +/- 5.9 per cent, respectively. The above mentioned granulocyte factors (GF) caused a statistically significant reduction of S cell activity (p less than 0.01) or even its complete inhibition, depending on the mitogen stimulating the R cells (this inhibition was greater after stimulation of lymphocytes by PWM). Inactivation of proteolytic enzymes with di-isopropylfluorophosphate (DFP) failed to decrease the biological activity of these factors, which are released from the granulocytes after 30 minutes of granulocyte adherence to Petri dishes. Lymphocyte culture in a medium containing the supernatant from a culture of 1 X 10(6)/ml adherent granulocytes demonstrated increased proliferation of these lymphocytes and reduced activity of suppressor cells. On the other hand, an increase in the number of adherent granulocytes to 5 X 10(6)/ml of the medium produced a reverse effect: impairment of the proliferative response and enhancement of the activity of S cells.

Adult↗
PubMedDate not supplied

[Cerebrospinal fluid cAMP and cGMP in various peripheral and central nervous system diseases. Preliminary report].

In a group of 51 patients the concentrations of cAMP and cGMP and their ratio were determined in the cerebrospinal fluid in certain neurological diseases. Statistically significant differences were found in the values of the concentrations of the above metabolites in selected groups of neurological diseases. Generally speaking, apart from a group of multiple sclerosis cases the values of cAMP concentration were higher in central nervous system diseases than in peripheral nervous system diseases. The highest values of the cAMP/cGMP ratio were observed in the group of patients with inflammatory conditions of the central nervous system. This method of cerebrospinal fluid examination may have an important role in the diagnosis of neurological diseases, particularly in multiple sclerosis.

Adult↗
PubMedDate not supplied

Concentrations of cAMP and activity of pertinent enzymes in certain brain structures of the rabbit.

The concentrations of cAMP and the activity of adenyl cyclase and specific cAMP-phosphodiesterase were determined in certain structures of rabbit brain. Statistically significant differences were found in the concentrations of cAMP; and enzyme activity between certain structures of the brain, particularly in the structures of the brain stem: mesencephalon, diencephalon and the pontine structures.

3',5'-Cyclic-AMP Phosphodiesterases↗