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J Crocker

Publications and source records attributed to J Crocker.

At least 181 records · Page 10Linked to original sources
PubMed1986

Presence of 3-fucosyl-N-acetyllactosamine shown by monoclonal antibody AGF 4.48 in Reed-Sternberg cells.

A series of 50 specimens of Hodgkin's disease and 10 of reactive follicular hyperplasia were examined by means of indirect immunoperoxidase staining with a monoclonal antibody AGF 4.48: this is known to bind to 3-fucosyl-N-acetyllactosamine, which, in particular, is expressed by granulocyte series cells. Most Reed-Sternberg and many Hodgkin's cells were labelled by the antibody after pretreatment with neuraminidase. Routinely processed paraffin wax embedded sections proved suitable for staining. The findings were comparable with those reported by others with monoclonal antibodies to various other granulocyte markers. This technique is of potential diagnostic value.

Antibodies, Monoclonal↗
PubMed1986

Ectopic beta-human chorionic gonadotropin production by bladder urothelial neoplasia.

Endocrine manifestations are rarely observed in bladder urothelial neoplasia. To our knowledge, only 11 cases of choriocarcinoma have been reported in this site, and recently the production of beta-human chorionic gonadotropin has been demonstrated in these tumors by applying the immunoperoxidase technique. Of 104 transitional cell neoplasms of the bladder, we demonstrated by the indirect immunoperoxidase technique beta-human chorionic gonadotropin production in 12 neoplasms, 11 of which were grade 3 and 4 transitional cell carcinomas.

Aged↗
PubMed1986

Immunohistochemical localization of S100 protein in skin tumors.

We applied a peroxidase-antiperoxidase technique for S100 protein to 73 tumors of skin and skin adnexa. These included 15 eccrine tumors, 11 apocrine tumors, 18 tumors with differentiation toward hair, two sebaceous adenomas, one mixed tumor of the scalp, ten dermatofibromas, ten basal cell carcinomas, five squamous cell carcinomas, and one clear cell acanthoma. Consistent results were obtained. Occasional cells in eccrine tumors showed strong positive staining, as did the Langerhans' cells in the squamous cell carcinomas and the clear cell acanthoma. The cells of the apocrine tumors showed moderate to weak staining, and the tumors with differentiation toward hair, the sebaceous adenomas, and the mixed tumor of the scalp showed uniform negative staining, as did basal cell carcinomas and dermatofibromas.

Humans↗
PubMed1985

The distribution of cathepsin B in human tissues.

Cathepsin B is a lysosomal enzyme of importance in many physiological and pathological processes. Its distribution in human tissues was studied by an indirect immunoperoxidase method. Cathepsin B was demonstrated in macrophages, hepatocytes, renal tubules, gastrointestinal epithelium and fibroblasts, confirming previous studies. It was demonstrated for the first time by immunohistology in several other tissues, especially stratified squamous epithelium, transitional epithelium, salivary glands, pancreas, central and peripheral neuronal cell bodies, trophoblast and all endocrine organs. Widespread distribution of cathepsin B has been postulated several times but this is the fullest evidence that the enzyme indeed occurs in many organs. In pathology cathepsin B has so far been thought to be involved in demyelination, emphysema, rheumatoid arthritis and neoplastic infiltration.

Cardiovascular System↗
PubMed1985

Immunohistochemical demonstration of S-100 protein in salivary gland neoplasms.

A peroxidase-antiperoxidase technique for S-100 protein has been applied to 68 salivary glands. These included 17 pleomorphic adenomas, seven adenoid cystic carcinomas, 23 adenolymphomas and a number of other neoplasms. In addition, five specimens of myoepithelial sialadenitis ('benign lymphoepithelial lesion') and five normal parotid glands were included. Consistent results were obtained, myoepithelial cells and cells in myxoid and chondroid areas in pleomorphic adenomas staining intensely. In adenoid cystic carcinoma, on the other hand, there was no staining. The adenolymphomas possessed intensely S-100 protein-positive cells in the interfollicular lymphoid areas; these were probably interdigitating reticulum cells. In addition, branching structures, probably corresponding to Langerhans' cells, were observed in the epithelium of adenolymphomas.

Adenolymphoma↗
PubMed1985

Another look at sex stereotypes and social judgments: an analysis of the social perceiver's use of subjective probabilities.

Locksley, Borgida, Brekke, and Hepburn (1980) assert that subjects fall prey to the base-rate fallacy when they make stereotype-related trait judgments. They found that subjects ignored their stereotypes when trait judgments were made in the presence of trait-related behavioral information. The present article reexamines those findings with respect to two issues: (a) the use of a normative criterion in comparison with subjects' judgments and (b) the level of analysis (group vs. individual) of subjects' judgments. We conducted a replication of the Locksley et al. (1980) Study 2, and the results were examined with respect to these two issues. We found no support for the base-rate fallacy. When a Bayesian normative criterion was constructed for each subject based on the subject's own stereotype judgments and was compared with assertiveness judgments made in the presence of individuating information, there was no evidence that subjects ignored or underused their stereotypes as the base-rate fallacy predicts.

Assertiveness↗
PubMed1985

Immunohistochemical localization of cathepsin G in human tissues.

An antiserum to human cathepsin G has been raised in sheep and its reactivity with human tissues has been tested. The indirect immunoperoxidase staining sequence was employed and was applied to routinely processed paraffin sections. Mature granulocytes, especially those of the neutrophil variety, were intensely and consistently stained. Activity was not observed in other cell or tissue types. Many of the cells of acute and chronic myeloid leukemia were strongly stained, in contrast to those of acute lymphoblastic or chronic lymphocytic leukemias. The results of the technique are compared with those described with staining for muramidase (lysozyme), alpha 1-antitrypsin, leukocyte elastase, and naphthol-AS-D-chloroacetate esterase, and with certain monoclonal antisera directed against granulocyte determinants.

Cathepsin G↗
PubMed1985

An immunohistochemical study of branchial cysts.

Twenty five specimens of branchial cyst from the same number of patients have been examined. On staining with haematoxylin and eosin a consistent finding was that the mural lymphoid follicles were always aligned with their mantle zones towards the luminal epithelium. With conventional staining lymphatic sinuses were noted in 17 of the specimens, but with immunohistochemical staining these structures were apparent in 23 cysts. Their frequent occurrence in branchial cysts supports the theory that these lesions are derived from epithelial inclusions in lymph nodes. Immunohistochemical techniques for a range of other markers, using polyclonal and monoclonal antisera, showed a distribution of lymphoid and non-lymphoid tissue elements, as seen in lymph nodes and, for example, palatine tonsils. The lining luminal epithelium also shared many features in common with the crypt epithelium of tonsils.

Adult↗
PubMed1985

Cells which contain S-100 protein in Hodgkin's disease: a quantitative study.

Paraffin sections from a series of 50 lymph nodes affected by Hodgkin's disease were examined by means of the unlabelled primary antibody peroxidase-antiperoxidase method to detect those cells which contained S-100 protein. In addition, 15 lymph nodes showing reactive follicular hyperplasia were studied. A simple enumeration procedure (eyepiece graticule) was used to count the number of such cells in 20 standard 25 X microscope fields. In the specimens of nodular sclerosing Hodgkin's disease many cells positive for S-100 protein were present, in contrast to the other Rye subtypes, which showed a relative paucity. By comparison, the lymph nodes showing reactive follicular hyperplasia contained a similar number of cells containing S-100 to those seen in the nodular sclerosing lymph nodes affected by Hodgkin's disease.

Cell Count↗
PubMed1984

An enzyme histochemical study of the sinuses of reactive lymph nodes.

The enzyme histochemistry of the cells lining and within the marginal and medullary sinuses of twenty human reactive lymph nodes has been studied. The sinuses contain luminal ('reticular') cells which are strongly positive for certain hydrolytic enzymes, including acid-alpha-naphthyl acetate esterase, acid phosphatase and beta-glucuronidase. In addition, the lining ('littoral') cells on both sides of the medullary sinuses are positive for these enzymes. In contrast, enzyme-containing lining ('littoral') cells of the marginal (subcapsular) sinuses are observed only on the inner aspect of the sinuses, the outer aspect being negative. Alkaline phosphatase is not present in the sinusoidal cells but 5'-nucleotidase is seen in varying amounts. These findings are supported by an ultrastructural study of three of the nodes, using a staining method for esterase activity. The different enzyme histochemical properties of the littoral cells in the marginal and medullary sinuses closely mirrors that observed when, for example, these structures are stained immunohistochemically for IgA or J chain.

5'-Nucleotidase↗
PubMed1984

Immunohistochemical demonstration of cathepsin B in the macrophages of benign and malignant lymphoid tissues.

Cathepsin B has been demonstrated by immunohistochemical means in the macrophages of palatine tonsils, reactive lymph nodes and in specimens of Hodgkin's disease (HD) and non-Hodgkin's lymphomas (NHL). Two cases of genuine histiocytic lymphoma showed strong staining for the enzyme in most cells. In Hodgkin's disease, many Reed-Sternberg cells and Hodgkin cells were positive. Branching and 'tingible body' macrophages (histiocytic reticulum cells, HRCs) were strongly positive in all of the specimens. In reactive lymph nodes, the sinus-lining cells and intrasinusoidal macrophages were positive for cathepsin B. True dendritic reticulum cells (DRCs) appeared to be negative. Unlike muramidase (lysozyme), cathepsin B is not seen in neutrophil polymorph leucocytes.

Cathepsin B↗
PubMed1984

Angiofollicular lymph node hyperplasia (Castleman's disease): an immunohistochemical and enzyme-histochemical study of the hyaline-vascular form of lesion.

Tissues from five cases of angiofollicular lymph node hyperplasia have been studied. All had the histological structure of the hyaline-vascular type of lesion; large numbers of very compact lymphoid follicles were distributed evenly throughout a highly vascular tissue. The follicles were characterized by their small size, a vascular poorly cellular and frequently hyalinized centre, and a 'tight' concentric mantle of small lymphocytes arranged in layers producing an 'onion-skin' appearance. The interfollicular tissue was characterized by the large numbers of small vessels mainly hyalinized capillaries and a few high endothelial venules and the presence of variable numbers of lymphocytes, plasma cells, immunocytes and immunoblasts. The immunoperoxidase method demonstrated polytypic cytoplasmic immunoglobulin in the small numbers of centroblasts and plasma cells within the follicle centres and in the plasma cells and immunocytes in the interfollicular tissue. Large numbers of suppressor T cells were present in the interfollicular areas and only scattered helper T cells were seen within the lymphocyte mantles. A strong reaction for factor VIII-related antigen was seen in the endothelium of the interfollicular high endothelial venules but only a weak reaction in the vessels in the follicle centres. A concentric distribution pattern of the dendritic reticulum cells was seen with the metalophil impregnation method of Marshall and with the enzyme histochemical methods for acid alpha-naphthyl acetate esterase and 5'-nucleotidase. This pattern differs from the zonal distribution of these cells seen in reactive lymphoid follicles. The nature and possible pathogenesis of AFLNH are discussed and contrasted with reactive hyperplasia.

5'-Nucleotidase↗
PubMed1984

Immunohistochemical localisation of factor VIII-related antigen in Hodgkin's disease.

A series of 45 lymph nodes involved by Hodgkin's disease have been examined by means of the unlabelled antibody peroxidase-antiperoxidase (PAP) method for the demonstration of factor VIII-related antigen (F VIII R Ag). In addition, five lymph nodes showing reactive follicular hyperplasia were studied. In the specimens of nodular sclerosing Hodgkin's disease, many blood vessels were present, as demonstrated by virtue of F VIII R Ag in endothelial cells. These vessels were present within and, especially, around the nodules in this subtype; in addition the fibrous trabeculae were densely vascular. High vascularity was also a feature of specimens of the cellular variant of nodular sclerosing Hodgkin's disease. However, the other three Rye subtypes of the disease showed a striking paucity of F VIII R Ag-positive blood vessels. The reactive nodes showed numerous vessels around and between follicles but the lymph sinuses were consistently negative. Mast cells were present in all specimens but were especially frequent within and adjacent to the sinuses in reactive specimens; they were strongly positive for the F VIII R Ag, their staining being abolished by pre-adsorption of the primary antiserum with factor VIII itself.

Adolescent↗
PubMed1984

Immunohistochemical demonstration of leucocyte elastase in human tissues.

A polyclonal antiserum, produced in sheep and reactive against purified human leucocyte elastase, has been applied to paraffin sections from a range of human tissues by means of the indirect immunoperoxidase method. Striking and consistent results have been obtained. Normal or inflammatory granulocytes were intensely positive, the reaction being "blocked" by pure elastase. Activity was not seen in other sites, including histiocytic reticulum and lymphoid cells, although some weaker reaction was present in gastric and ileal lining epithelium. Strong reactivity was also seen in the cells of acute and chronic myeloid leukaemia and in extramedullary haemopoiesis. The advantages of this technique are compared with those for muramidase (lysozyme), alpha 1-antitrypsin, and naphthol-AS-D-chloroacetate esterase.

Granulocytes↗
PubMed1984

A quantitative study of histiocytic reticulum cells in diffuse and follicular non-Hodgkin's lymphomas.

Histiocytic reticulum cells have been counted in 160 lymph nodes, comprising 50 high grade non-Hodgkin's lymphomas, 90 lymphomas of low grade histology, and 20 specimens exhibiting reactive follicular hyperplasia. The histiocytes were shown immunohistochemically by virtue of their content of the cysteine proteinase cathepsin B. A consistent and striking finding was that high grade lymphomas contain many more histiocytes than low grade lymphomas. Immunoblastic neoplasms contain up to 24.2% of these cells, whereas low grade diffuse lymphomas possess only up to 3.6% histiocytes. Histiocytic reticulum cells were also counted in benign or malignant follicular lesions in standard areas from follicle centres only. No significant differences were found between low grade lymphomas and hyperplastic nodes. These findings are discussed in relation to previous, more limited studies.

Cathepsin B↗