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J Correa

Publications and source records attributed to J Correa.

At least 37 records · Page 2Linked to original sources
PubMed1995

Computer-assisted diagnosis: the classification of mammographic breast parenchymal patterns.

We have developed a method for the quantification of breast texture by using different algorithms to classify mammograms into the four patterns described by Wolfe (N1, P1, P2 and Dy). The computerized scheme employs craniocaudal views of conventional screen-film mammograms, which are digitized by a laser scanner. We used discriminant analysis to select among different feature-extraction techniques, including Fourier transform, local-contrast analysis, and grey-level distribution and quantification. The method has been evaluated on 117 clinical mammograms previously classified by five radiologists as to mammographic breast parenchymal patterns (MBPPS). The results show differences in agreement among radiologists and computer classification, depending on the Wolfe pattern: excellent for Dy (kappa = 0.77), good for P2 (kappa = 0.52) and N1 (kappa = 0.52) and poor for P1 (kappa = 0.22). Our quantitative texture measure as calculated from digital mammograms may be valuable to radiologists in their assessment of MBPP and therefore useful in establishing an index of risk for developing breast carcinoma.

Algorithms↗
PubMed1995

Digital chest radiography: comparison of unprocessed and processed images in the detection of solitary pulmonary nodules.

PURPOSE: To compare the accuracy with which simulated solitary pulmonary nodules can be identified on digital images of the chest that are unprocessed, processed with adaptive spatial filtering, or processed with global filtering. MATERIALS AND METHODS: Six experienced chest radiologists evaluated 408 test radiographs (136 from each of the three types, half with simulated nodules) and judged whether a nodule was present. Data from the 2,448 observations were evaluated by means of a receiver operating characteristic curve with location methods. RESULTS: Accuracy was significantly better with the adaptive filter technique than with the global technique (P < .05), and it was better with adaptive filtering than with no processing in the detection of pulmonary nodules in the mediastinal-subdiaphragmatic areas (P < .05). No significant difference was found between no processing and global filtering. CONCLUSION: Adaptive filtration is superior to global filtration in the identification of solitary pulmonary nodules and is superior to no processing in nodules projected over the radiopaque areas of the thorax on digital images.

Algorithms↗
PubMed1994

Regulation of the cell cycle timing of Start in fission yeast by the rum1+ gene.

We have identified the rum1+ gene as a new regulator of the G1-phase of the fission yeast cell cycle. rum1+ determines the cell cycle timing of Start, by maintaining cells in a pre-Start state until they have attained a minimal critical mass. Cells lacking rum1+ are unable to arrest in pre-Start G1 in response to nitrogen starvation and are subsequently sterile. In addition, rum1+ prevents entry into mitosis from pre-Start G1, as shown by the fact that cdc10 mutants in the absence of rum1+ undergo lethal mitosis without entering S-phase.

Cell Cycle↗
PubMed1993

SSG1, a gene encoding a sporulation-specific 1,3-beta-glucanase in Saccharomyces cerevisiae.

In Saccharomyces cerevisiae, the meiotic process is accompanied by a large increase in 1,3-beta-glucan-degradative activity. The molecular cloning of the gene (SSG1) encoding a sporulation-specific exo-1,3-beta-glucanase was achieved by screening a genomic library with a DNA probe obtained by polymerase chain reaction amplification using synthetic oligonucleotides designed according to the nucleotide sequence predicted from the amino-terminal region of the purified protein. DNA sequencing indicates that the SSG1 gene specifies a 445-amino-acid polypeptide (calculated molecular mass, 51.8 kDa) showing extensive similarity to the extracellular exo-1,3-beta-glucanases encoded by the EXG1 gene (C. R. Vazquez de Aldana, J. Correa, P. San Segundo, A. Bueno, A. R. Nebreda, E. Mendez, and F. del Rey, Gene 97:173-182, 1991). The N-terminal domain of the putative precursor is a very hydrophobic segment with structural features resembling those of signal peptides of secreted proteins. Northern (RNA) analysis reveals a unique SSG1-specific transcript, 1.7 kb long, which can be detected only in sporulating diploids (MATa/MAT alpha) but does not appear in vegetatively growing cells or in nonsporulating diploids (MAT alpha/MAT alpha) when incubated under nitrogen starvation conditions. The meiotic time course of SSG1 induction indicates that the gene is transcribed only in the late stages of the process, beginning at the time of meiosis I and reaching a maximum during spore formation. Homozygous ssg1/ssg1 mutant diploids are able to complete sporulation, although with a significant delay in the appearance of mature asci.

Amino Acid Sequence↗
PubMed1993

Novobiocin forms cation-permeable ion channels in rat fetal distal lung epithelium.

The antibiotic novobiocin has been previously reported to increase Na+ transport in frog skin, presumably by attenuation of Na+ self-inhibition of Na+ channels. To determine whether novobiocin had similar effects and utilized a similar mechanism in mammalian Na(+)-transporting tissues, we studied its effect on ion transport by primary cultures of fetal distal lung epithelium (FDLE) cultured from 20-day gestationally aged rats (term = 22 days). Novobiocin (10 mM) increased short-circuit current and markedly decreased the resistance in FDLE monolayers mounted in Ussing chambers. Fura-2 single-cell studies showed that novobiocin increased intracellular Ca2+ concentration and that this resulted from extracellular sources. Nystatin-perforated patch-clamp techniques demonstrated that novobiocin increased nonrectifying cation whole cell currents without inducing detectable anion currents. Novobiocin created nonrectifying monovalent cation-selective channels in lipid bilayers. These studies demonstrated that novobiocin affects the bioelectric properties of Na+ transporting lung epithelium and that this likely occurs by the formation of ion-permeant channels in their lipid membranes.

Animals↗
PubMed1992

Genetic mapping of 1,3-beta-glucanase-encoding genes in Saccharomyces cerevisiae.

The map position of three 1,3-beta-glucanase-encoding genes in S. cerevisiae has been determined following conventional meiotic and mitotic mapping combined with recombinant DNA techniques. EXG1, EXG2 and SSG1 were localized to chromosomes XII, IV and XV, respectively, by hybridizing the cloned genes to Southern blots of chromosomes separated by pulsed-field gel electrophoresis, in conjunction with the rad52-1-dependent chromosome-loss mapping technique. Meiotic tetrad analyses further localized the EXG1 gene 6.1 centimorgans centromere-proximal to CDC25 on the right arm of chromosome XII. EXG2 was positioned between LYS4 and GCN2 on the right arm of chromosome IV, at distances of 6.2 centimorgans from LYS4 and 4.9 centimorgans from GCN2. Finally, the SSG1 locus mapped on the right arm of chromosome XV, about 8.2 centimorgans to the centromere-proximal side of HIS3.

Chromosome Mapping↗
PubMed1992

Enhancement of chest images by automatic adaptive spatial filtering.

Postprocessing of the image data is an exciting capability of digital radiography that may improve diagnostic performance. We present a new algorithm that selectively enhances edges and contrast in both lungs and mediastinum while minimally amplifying noise in chest images. Using different size kernels, two smoothed images are generated from the original chest image. The two regions of interest (lungs and mediastinum) are identified based on the distribution of pixel values in the image. A modified nonlinear unsharp mask subtraction technique is then applied. The resulting image has enhanced high- and middle-frequency information in the mediastinum without distorting lung parenchyma or significantly enhancing noise. We consider that the technique employed in this study could be suitable for routine use although its true effectiveness in improving diagnostic accuracy awaits observer-performance evaluation that is currently under way.

Adult↗
PubMed1991

Nucleotide sequence of the exo-1,3-beta-glucanase-encoding gene, EXG1, of the yeast Saccharomyces cerevisiae.

The nucleotide (nt) sequence of the Saccharomyces cerevisiae gene (EXG1) encoding extracellular exo-1,3-beta-glucanases (EXG) I and II was determined. An open reading frame of 1344 bp codes for a 448-amino acid (aa) polypeptide, with a calculated Mr of 51,307, which contains two potential N-glycosylation sites. The EXG1 DNA hybridizes to a 1.7-kb transcript whose 5' end maps to a position 98 bp upstream from the site of initiation of protein synthesis. Comparison of the N-terminal aa sequence deduced from the nt sequence with that of the purified EXGII revealed the existence of an extra 40-aa peptide in the precursor protein containing a Lys-Arg peptidase-processing site at the junction with the mature, extracellular form. The N-terminal region of the putative precursor is a very hydrophobic segment with structural features resembling those of signal peptides of secreted proteins. The Mr of the mature EXG polypeptide deduced from the nt sequence is 46,385. The 5'- and 3'-flanking regions of the EXG1 gene have structural features in common with other yeast genes.

Amino Acid Sequence↗
PubMed1990

Synthesis and secretion of a Bacillus circulans WL-12 1,3-1,4-beta-D-glucanase in Escherichia coli.

The synthesis and secretion of a 1,3-1,4-beta-D-glucanase were studied in different strains of Escherichia coli transformed with plasmids carrying the Bacillus circulans WL-12 1,3-1,4-beta-D-glucanase structural gene. This gene (named BGC) is contained within a 1.9-kilobase BamHI-HindIII fragment and directs the synthesis in E. coli of an enzyme that specifically degrades lichenan. Only one active form of the enzyme was found when the gene was expressed in different E. coli strains. The electrophoretic pattern of this protein showed a molecular weight that was approximately the same as that of the mature beta-glucanase secreted from B. circulans WL-12, suggesting that the processing of this protein may be similar in both species. As deduced from maxicell experiments, the Bacillus parental promoter directs the synthesis in E. coli. Pulse-chase experiments showed that the protein may be cotranslationally processed.

Bacillus↗
PubMed1990

The 25-kilodalton insulin-like growth factor (IGF)-binding protein inhibits both basal and IGF-I-mediated growth of chick embryo pelvic cartilage in vitro.

Insulin-like growth factor (IGF)-I stimulates the growth of many tissues, including growth plate cartilage. However, the role of IGF-binding proteins in the growth process is controversial. We purified a 25-kDa IGF-binding protein (BP-25) from amniotic fluid. We tested the effect of this BP-25 preparation on both basal and IGF-I-stimulated growth of chick embryo pelvic cartilages maintained in serum-free organ culture. Cartilage wet weight was 4.1 +/- 0.3 mg/cartilage initially; after 3 days, BP-25 inhibited both basal and IGF-I-stimulated growth. Control cartilages weighed 7.4 +/- 0.7 mg/cartilage, while those incubated with 100 nM BP-25 weighed 5.8 +/- 0.5 mg/cartilage (P less than 0.001 vs. control); BP-25 concentrations as low as 0.2 nM significantly inhibited basal cartilage growth. Cartilages incubated with 1.25 nM IGF-I weighed 10.4 +/- 0.8 mg/cartilage (P less than 0.001 vs. control), while those incubated with both 100 nM BP-25 and 1.25 nM IGF-I weighed 8.1 +/- 0.5 mg/cartilage (P less than 0.001 vs. cartilage incubated with IGF-I alone); BP-25 concentrations as low as 0.4 nM significantly inhibited IGF-I-stimulated cartilage growth. BP-25 also inhibited basal and IGF-I-stimulated increases in cartilage dry weight, [3H]thymidine incorporation into DNA, and 35SO4 incorporation into proteoglycan. A second BP-25 preparation, which in the presence of 1% platelet-poor plasma acts synergistically with IGF-I to stimulate DNA synthesis and cell replication of fibroblasts and smooth muscle cells in tissue culture, inhibited IGF-I-stimulated cartilage growth to the same degree as did our BP-25 preparation. In separate experiments, proteins present in serum-free medium conditioned for 3 days by chick cartilages were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and incubated with [125I]IGF-I. This medium was found to contain two IGF-binding proteins; one appeared to be the chick equivalent of BP-25, while the other had a molecular mass similar to that of a poorly characterized human 34-kDa IGF-binding protein. We conclude that purified BP-25 inhibits the growth of chick embryo pelvic cartilage in our serum-free organ culture system. Since conditioned medium from these cartilages contains both IGF-I-like peptides and IGF-binding proteins such as BP-25, we suggest that the IGF-binding proteins present may act to down-regulate the growth-promoting effects of the local IGF peptides.

Animals↗
PubMed1989

Axonal patterns of disc-shaped cells in the central nucleus of the cat inferior colliculus.

The axonal patterns of disc-shaped cells (Dsc) and their distribution within the central nucleus (Cn) of the inferior colliculus was studied in young cats with the rapid Golgi method. Dsc were subdivided in three main cell varieties according to their axonal branching pattern. The first type possesses local axonal collaterals inside the lamina of origin but also gives collaterals and probably terminals to adjacent lamina. The second variety is characterized by a dense axonal plexus with a restricted zone of arborization. The third axonal pattern is distributed in a radiate fashion. These results demonstrate that Dsc contribute to the intrinsic axonal system of the Cn to a larger degree than previously supposed. Axon terminals of Dsc probably establish axo-dendritic contacts with medium sized Stc which probably establish contacts with neighbouring Dsc. This suggests that reciprocal connections between Dsc and Stc could exist in the Cn.

Animals↗
PubMed1988

A prospective randomized trial of fluorouracil versus fluorouracil plus cisplatin in the treatment of metastatic colorectal cancer: a Hoosier Oncology Group trial.

From May 1984 through December 1986, 141 patients with metastatic adenocarcinoma of the colon or rectum were entered on this Hoosier Oncology Group (HOG) trial evaluating the role of cisplatin in systemic therapy. Patients were stratified by the presence or absence of hepatic metastases and by performance status, and were subsequently randomized to receive fluorouracil (5-FU) (15 mg/kg/wk) alone or the same dose of 5-FU plus cisplatin (60 mg/m2 every 3 weeks). The total duration of treatment was six cycles (18 weeks). In 132 fully evaluable patients the objective response rates were 19% for 5-FU and 22% for 5-FU plus cisplatin. Statistically, the median survival times of 40 and 39 weeks were not significantly different (P = .62). However, the median duration of remission (MDR) was superior (P = .05) for 5-FU alone. This study fails to confirm clinically significant synergy of 5-FU plus cisplatin in the treatment of metastatic colorectal cancer.

Adult↗
PubMed1988

Stellate cells and their axonal patterns in the central nucleus of the inferior colliculus of the cat (Felis domesticus).

Stellate cells (Stc) in the central nucleus of the inferior colliculus of the cat are divided according to cell body size and shape, dendritic branching pattern and axonal arborization into large, medium-size and small categories. Each of these were divided in different cell types. Three types of large Stc, six types of medium sized Stc and one type of small Stc were described. All of these cell types differ from each other in their morphological characteristics. Stc contribute to the neuropil organization of the central nucleus with numerous axonal collaterals which differ in their form and extension. Large and medium-sized Stc could be projecting neurons with a system of local axonal collaterals while the small Stc is considered a Golgi II type neuron. The probable synaptic relationships between Stc and disc-shaped neurons as well as between the different types of Stc are described. The mode of this probable synaptic arrangement suggests that another level of organization exist in the central nucleus, superimposed to the anatomically defined fibrodendritic laminae.

Animals↗
PubMed1985

Efficacy of ipronidazole against trichomoniasis in beef bulls.

Preputial smegma samples from 195 beef bulls were collected repeatedly and cultured for Tritrichomonas foetus. Seventy-five (38.5%) of these bulls were positive for trichomonads on at least 1 culture. Sensitivity of the culture procedure (number of positive cultures/number of total cultures from known-positive bulls) was 81.6%. Storage of preputial smegma in lactated Ringer's solution at 5 C for 24 hours resulted in a 14% loss of sensitivity. Seventy-three of the 75 infected bulls were available for treatment and were alloted randomly to 2 groups. Bulls in both groups were treated with procaine penicillin (7,000 IU/kg, IM) for 2 days before ipronidazole treatment. Thirty grams of ipronidazole powder was dissolved in 60 ml of sterile water, and was given IM to group 1 bulls. Group 2 bulls were given a similar 30-g ipronidazole solution IM on day 1, and were given 15 g of ipronidazole dissolved in 30 ml of sterile water on days 2 and 3. Efficacy of treatment (ie, negative cultures of preputial smegma for trichomonads for 6 consecutive weeks after treatment) was 92.8% for the 42 bulls treated once and 100% for the 31 bulls treated 3 times.

Animals↗