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Biomedical subjects

J Conde

Publications and source records attributed to J Conde.

At least 91 records · Page 5Linked to original sources
PubMed1981

[Analysis of germ cultures in 57 cases of appendicular peritonitis and 16 postoperative septic complications].

Reliable bacteriological data were obtained from 53 and 16 out of 64 pediatric appendiceal peritonitis an 17 of their suppurative complications. Studies for aerobic and anaerobic flora ws performed in all. Each primitive peritoneal exudate contained a mixed flora with an average of 1.3 aerobic and 2.27 anaerobic species. E. coli and B. fragilis were almost constantly found among other enteric bacteria. Exudates from wound or intraabdominal complicative infections contained an average of 1.3 aerobic and 3 anaerobic species, and again E. coli and B. fragilis were constantly present. In eleven patients, there were exudates available from both the peritonitis and the complications, and the flora was coincident for, at least, one gram in all, and for two or more in eight. These facts enable us to point out the importance of anaerobes in peritoneal infections and their complications. At the same time, we recall that antibiotic treatment of peritonitis must be prescribed with this evidence in mind.

Abscess↗
PubMed1980

[Infection of pediatric operative wounds in a general hospital (author's transl)].

During the two-year 1977-1978 period 37 (3.29%) out of 1,124 pediatric surgical wounds became infected in the postoperative course. Ten infections were observed among the 844 "clean" procedures (1.18%), 5/155 "clean-contaminated" (3.22%), 3/32 "contaminated" (9.37%) and 19/93 "dirty" (20.43%). These rates are quite comparable, although somewhat lower, to those found in similar studies carried out in adults. The bacteriologic studies of the contamination and the subsequent infection revealed that "clean" wounds were almost exclusively infected by "Staphilococci", while all the remaining categories (which very often implicated an opening of digestive tract lumen) became infected by multiple endogenous flora including enterobacteriae (mainly "E. coli") and anaerobic germs ("Bacteroides" and "Clostridia"). The limited use of antibiotics in this series was guided by these bacteriologic findings, a fact that can partially explain our results. There was no mortality directly related to the infection of the wound, but the risks and uncomfort incurred by the patients were not negligible and must be taken into consideration aside with the increase of the cost of hospital stay (evaluated as 35,000-100,000 ptas./patient in this series) to justify every effort to decrease incidence of this generally minor complication.

Adult↗
PubMed1979

Saccharomyces cerevisiae mutant defective in exo-1,3-beta-glucanase production.

Saccharomyces cerevisiae S288C produced two laminarinases (1,3-beta-glucanases) which were separated by diethylaminoethyl-Sephadex column chromatography; one was an endo-1,3-beta-glucanase, and the other was an exo-1,3-beta-glucanase active not only on laminarin but also on pustulan (1,6-beta-glucan) and on p-nitrophenyl-beta-D-glucoside. A mutant defective in the production of this last enzyme was isolated, and the mutation was named exb1-1. The selection procedure was based on the capacity of exo-1,3-beta-glucanase to hydrolyze synthetic glucosides. The level of endo-1,3-beta-glucanase in cell extracts of the mutant was normal, but the exo-1,3-beta-glucanase could not be detected by column chromatographic analysis of these extracts. The mutant phenotype, recessive in heterozygous diploids, was stable through successive meioses and showed a Mendelian segregation, indicating that the mutation affected a single gene, which was named EXB1. The lack of production of exo-1,3-beta-glucanase persisted through all the phases of growth, but growth itself was not impaired by the enzyme deficiency.

Genes↗
PubMed1976

A mutant of Saccharomyces cerevisiae defective for nuclear fusion.

A mutant unable to fuse nuclei during mating has been isolated from standard wild-type Saccharomyces cerevisiae. Tetrad analysis of the mutation responsible for this defect (kar1-1) shows that it segregates as a single Mendelian factor. The defect kn kaf1-1 appears to be nuclear limited. Cytological and genetic evidence shows that in this mutant the events associated with zygote formation are normal until the point of nuclear fusion. The consequence of this defect is the formation of a multinucleate zygote which in subsequent divisions can segregate heterokaryons and haploid heterplasmons.

Cell Fusion↗
PubMedDate not supplied

Tolerance and short-term effect of a cluster schedule with pollen-extracts quantified in mass-units.

We performed a prospective, multicenter study to assess the tolerance and possible short-term effects of allergen vaccines administered according to a cluster schedule in the months immediately preceding the onset of the pollen season. The study was carried out in eight centers and included 191 patients (children and adults) with allergic respiratory disease due to sensitization to olive tree and/or grass pollen. Of these, 34 patients acted as controls and the remaining patients received immunotherapy administered in the initiation phase according to a cluster schedule of eight doses injected on four visits. After 3 months of treatment, significant differences were found between the two groups in medication consumption (antihistamines in drops and oral formulations: p = 0.045 and p = 0.001, respectively; short-acting beta2-agonist treatments: p = 0.004) and respiratory symptoms (wheezing and coughing: p = 0.035 and 0.014, respectively). The cytokine profile (interleukin [IL]-4, 5, 10 and 2, interferon [IFN-gamma], and tumor necrosis factor [TNF-alpha]) was determined before the start of treatment and at the end of follow-up (4-5 months). Levels of IL-4, 5 and 10 (Th2 profile) decreased while those of IL-2, IFN-gamma, and TNF-alpha (Th1 profile) decreased. These differences were more marked in the active group than in the control group but were not statistically significant. No severe adverse effects were recorded. This study shows that the schedule tested had an acceptable tolerance profile and produced significant changes in symptom and medication scores after a few months of treatment. A double-blind, placebo-controlled study is needed to confirm these results.

Adult↗
PubMedDate not supplied

Plasma kallikrein amidolytic activity in bronchial asthma.

We have investigated plasma kallikrein amidolytic activity in the following groups of patients: 1. Normal control group of blood donors. 2. Extrinsic pollen-activated bronchial asthma patients, during periods of symptomatology and at a later time after the spring. 3. Subjects with atopic bronchial asthma in acute phase when admitted to our hospital's emergency room and later when clinically recovered. 4. Subjects with extrinsic bronchial asthma, sensitive to Dermatophagoides pteronyssinus and Dermatophagoides farinae with FEV1 < 80%. 5. Subjects with extrinsic bronchial asthma, sensitive to Dermatophagoides pteronissinus and Dermatophagoides farinae in a state of clinical rest. After 9 minutes of activation, the following results were found, with a significance of p < 0.01: There are significant differences between the normal group and those that we consider the active groups, asthma FEV1 < 80%, pollen-sensitive asthma in springtime and acute asthma. No significant differences exist between the normal group and inactive groups, inactive asthma, pollen-sensitive asthma out of springtime and acute asthma inactive. Significant differences exist in active groups (acute asthma and pollen-sensitive asthma in springtime) when they become inactive (acute asthma inactive and pollen-sensitive asthma out of springtime). The active groups have a higher plasma kallikrein amidolytic activity than both the inactive and control groups.

Acute Disease↗
PubMedDate not supplied

The study cellular subpopulations in peripheral blood from a normal reference group population (blood donors).

The spectacular development of monoclonal antibodies against cellular antigens an technology such as flow cytometry allow the investigation of cellular subpopulations that until now have been unknown. At the same time, the functional study of these subpopulations becomes of maximal interest, as this information could have future applications for pathological processes. Due ti this basic need for information, we have studied diverse lymphocytic subpopulations in a normal population that serves as a reference group, using the following antigens: CD3, CD4, CD8, CD20, CD45RA, CD25, LAM1, CD29, CD11b and CD23. Some of these subpopulations had not been previously studied in a normal reference group.

Antibodies, Monoclonal↗