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Biomedical subjects

J Coll

Publications and source records attributed to J Coll.

At least 109 records · Page 6Linked to original sources
PubMed1982

Characterization of c-lil, a chicken cellular sequence associated with a stock of B77 avian sarcoma virus.

Using biochemical methods, we have shown that a new specific sequence, v-lil, is associated with a given stock of B77 avian sarcoma virus (clone 9). We prepared a DNA complementary to v-lil sequences, using substractive hybridizations, and investigated the properties of this sequence. v-lil has a genetic complexity of ca. 2,000 nucleotides and is not present in various stocks of avian sarcoma virus, avian leukosis virus, or defective leukemia virus. v-lil is not associated with B77 avian sarcoma virus isolated from the original tumor and thus has been acquired by in vitro passage of the virus on chicken embryo fibroblasts. A search for the origin of the v-lil sequence among the DNAs of different avian species has shown that a similar sequence, c-lil, is present in normal chicken DNA (1 to 2 copies per haploid genome). c-lil is not highly conserved but is present in the DNA of all chickens from the genus Gallus. The c-lil sequence is transcribed at a low level (1 to 3 copies per cell) in normal chicken embryo fibroblasts. The biological function, if any, of v-lil or its cellular equivalent has yet to be determined.

Animals↗
PubMed1982

[Circulating immune complexes in the serum of tumor patients].

The conditions for the detection of circulating immune complexes (CIC) were established by combination of polyethylene glycol precipitation with a nephelometric determination on the precipitate of endogenous C1q. The CIC precipitated from the sera of patients with breast carcinoma, malignant melanoma or autoimmune diseases were dissociated in SDS-beta-mercaptoethanol buffer and no differences were observed in the electrophoretic patterns os polyacrylamide gels allowing to characterize tumour specific antigens.

Antigen-Antibody Complex↗
PubMed1981

Pulmonary involvement in primary biliary cirrhosis.

The association of pulmonary fibrosis and primary biliary cirrhosis (PBC) remains controversial. To determine the frequency of pulmonary fibrosis in PBC, a carefully selected series of 14 PBC patients, seven patients with Sicca complex, and 14 control subjects have been studied. Seven of the 14 patients with PBC had Sjögren's syndrome, four of whom had some clinical evidence of pulmonary disease. Evaluation of ventilatory capacity, gas transfer factor, arterial blood gases, and lung mechanics were performed. Gas transfer was reduced in patients with PBC associated with Sjögen's syndrome and in patients with the Sicca complex. These results suggest that the respiratory, clinical, ad functional abnormalities found in PBC are related to the presence of an associated Sjögen's syndrome.

Adult↗
PubMed1981

Incorporation of fatty acids into phosphatidylcholine species by liver homogenates from chick embryos.

Labeled palmitic, stearic, oleic, linoleic and araquidonic acids were incorporated into phosphatidylcholines by incubation with liver homogenates from 13, 17 and 21 day-old chick embryos. Palmitic acid was incorporated into dienoic and tetraenoic phosphatidylcholines whereas linoleic acid was incorporated into dienoic phosphatidylcholines. Labeled palmitic and linoleic acids were specifically found into positions 1 and 2 of the phosphatidylcholine molecule, respectively. Under in vitro conditions, the incorporation of either palmitic or linoleic acids into phosphatidylcholines was 2-3 fold greater in liver homogenates from 17 day-old chick embryos than in those from 13 or 21 day-old ones.

Age Factors↗
PubMed1981

Deacylation and reacylation of phosphatidylcholines by liver homogenates from chick embryos.

Liver homogenates from 13, 17 and 21 day-old chick embryos were incubated with phosphatidylcholine and lysophosphatidylcholine labeled with fatty acids to study deacylation and reacylation reactions of phosphatidylcholines during development. By using phosphatidylcholines labeled with palmitic or linoleic acids in position 1 or 2, respectively, phospholipase A2 activity was detected. By using lysophosphatidylcholine labeled with palmitic acid in position 1, preferential acylation with araquidonic acid was demonstrated. In agreement with this data, the incubation of dienoic phosphatidylcholines labeled with palmitic acid in position 1, with the liver homogenates, gave rise to labeled tetraenoic phosphatidylcholines. The extent of both deacylation and reacylation of the phosphatidylcholine molecule in position 2 by liver homogenates from chick embryos, decreased during development.

Acylation↗
PubMed1980

[Urinary tract infection in postmenopausal diabetic women. Clinical survey of hospital and nonhospital populations (author's transl)].

The incidence and clinical course of urinary tract infections in postmenopausal diabetic patients in comparison to non-diabetic women have been evaluated. All cases with former history of urinary instrumentation or underlying conditions favoring urinary infections were rejected. Urinary tract infection was present in 14.9 percent of postmenopausal diabetics versus 10.5 percent in the non-diabetics. The incidence of urinary tract infection in premenopausal women was 12.5 percent. Urinary pathogenes showed similar incidence rate in both groups: E. coli (41 versus 57 percent), P. mirabilis (20 versus 28 percent) and Klebsiella (11 versus 20 percent). Diabetic women showed a higher rate of sepsis and infection recurrence than non-diabetic (10.7 and 17.8 percent versus 2.6 and 2.6 percent, respectively). Severe impairment of renal function (plasma creatinine clearance > 2 mg/100 ml) was registered in 12.5 percent of postmenopausal patients with diabetes mellitus, but none of the non-diabetics suffered renal damage (p < 0.01).

Aged↗
PubMed1979

Pulpectomy procedure for deciduous teeth with severe pulpal necrosis.

Pulpectomies of deciduous teeth with severe pulpal necrosis should be considered as a possible treatment plan. Systemic and dental criteria have been devised to help the clinician to select cases in which successful results may be produced. A clinical success occurs when the pulpectomized tooth is painless, is firm in its alveolar socket, and is without a fistulous tract. Radiographically, any radiolucent area should be resolving with six months, and no pathologic root resorption should be observed. Research should determine whether pulpectomies may be done on children with severe systemic disease and should determine long-term consequences of underfilled and overfilled pulpectomies.

Dental Pulp Capping↗