Rapid DNA isolations for enzymatic and hybridization analysis.
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Biomedical subjects
Publications and source records attributed to J Cameron.
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In Salt Lake County, Utah in 1972 and 1975, 167 women had planned home deliveries. Birth certificate data indicated they did not differ from the total population in age, marital, and socioeconomic status. Eighty-four women were interviewed and reported hostility from health professionals which may have placed them at unnecessary risk.
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In the first three years of a Nurse Specialist Clinic in Family Planning, 1422 new patients were seen. Oral contraception (OCs) was dispensed for 638 patients, 548 intrauterine devices (IUDs) were fitted and 126 patients received an injectable contraceptive. The continuation rate at thirty months was, 73.2 for OCs, and 68.6 for IUDs. The additional training received by the nurse specialists allowed them to practice comprehensive family planning safely and effectively, as shown by the low "problem" rate, 9.1% for OCs and 7.2% for IUDs. Continued use effectiveness, as shown by the second choice of contraception initiated in the clinic for those who stopped using their first method, was also high, 81.5% for OCs and 90.5% for IUD closures.
In a clinic population of 289 children (nine years of age and under) suspected of developmental delay, it was found that correlations on repeated administrations of the Bayley and/or Stanford-Binet intelligence tests were much higher than in normal children; that the magnitude of the correlations was inversely related to IQ level; that children initially tested under two years of age displayed more IQ change than those tested over the age of two, with girls more likely than boys to show a drop, and the greatest amount of fluctuation occurring the 51-80 range. The IQ constancy of this population has important developmental implications for the retarded: it suggests that they are growing intellectually at a rate proportional to normal children of their own mental rather than chronological age; that they may well have more years of maturation before reaching a plateau; and that educational programs should take into consideration this lengthened learning period.
The desirability of choosing methods and equipment to meet local needs such as yield per cycle and ready availability of components of media and other reagents, and of simple equipment such as good quality glassware, of replacement parts for more complex equipment, and, most of all, of technical assistance is emphasised. Particular emphasis is given to ways of increasing yield from agar and static-liquid culture and to simple methods of separating bacteria from large volumes of culture using bacterial-precipitants. Designs are given for simple growth and mixing vessels, including magnetically-stirred units which require a minimum of servicing. It is strongly recommended that the basic production-unit should be a simple, multipurpose, high-quality stainless-steel vessel of appropriate size: such a unit has a working-life of at least 10 years. Regardless of the type of equipment used, simple or complex, the need, at all times, for well-trained technical staff is also stressed.
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When a tracheal-innominate artery fistula is suspected, angiography is essential if the patient's condition is stable. An aggressive angiographic approach is needed to demonstrate the fistulous tract. Multiple injections in different degrees of obliquity and subtraction views should be obtained for early accurate diagnosis and for optimal demonstration of the pathologic anatomy.
The present paper indicates a number of unresolved problems in the metabolism of Bordetella pertussis and in the standardization of pertussis vaccines. The use of chemically defined media should enable the metabolic problems to be clarified. The problems concerned with the control and standardization of vaccines can largely be resolved in the laboratory with the introduction of reference preparations but the question of the reactivity of vaccine remains for the future.
Reversion of toxoid to toxin has been described by several authors yet present day regulations of all National Control Authorities have no specific requirements to check for this possibility. Most producers however have included some form of test in their own protocols for release. We have developed a satisfactory test for both diphtheria and tetanus toxoids which involves storing diluted toxoid at 20 degrees C and 34 degrees C for 6 weeks and performing a full toxicity test on the samples. In the case of diphtheria toxoid we have evidence that it may be dangerous to rely on the simpler guinea-pig skin test, since we have shown that several lots, while giving a negative skin reaction, exhibit diphtheria toxicity when given systemically. It is felt that an in vivo conversion to toxin may occur in some lots which would not be detected by the skin test.
Toxicity tests based on mouse-weight-gain (whether control of pertussis, DPT, cholera or typhoid vaccine) are not satisfactory, particularly since the vaccines may be adsorbed and non-adsorbed preparations. Comparison between results of tests in animals some of which were treated with vaccine and others with saline does not allow to make conclusions as valid as those supplied by a standard control vaccine (adsorbed and not adsorbed) instead of saline.
Cell death accompanies the formation of free digits in birds and mammals. However, in species with webbing between the adult digits, little or no cell death occurs in the prospectively webbed region of the developing interdigit. Cell death does not occur during the formation of free digits in amphibians. In this paper we report that cell death accompanies the formation of the digits in snapping and painted turtles and in the skink (a lizard). We conclude that cell death accompanying the formation of free digits had its origin at the point of amniote emergence during evolution.
In an experimental clinic, run by nurse specialists in family planning, a total of 768 patients were seen in the first year. Oral contraception was dispensed for 377 patients and 187 intrauterine devices (IUCDs) were inserted; a further 204 IUCD patients attended only for follow-up visits. All side effects were adequately diagnosed by the nurse specialist.