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J C Monier

Publications and source records attributed to J C Monier.

At least 91 records · Page 5Linked to original sources
PubMed1982

Identification of FTS (facteur thymique serique) on thymus ultrathin sections using monoclonal antibodies.

Using a new immunoelectromicroscopical technique, this study confirms the localization of the 'facteur thymique sérique' (FTS) in the reticulo-epithelial cells of mouse thymus. The use of anti-FTS monoclonal antibodies on ultrathin sections for electron microscopy reveals FTS in cytoplasmic vacuoles, labelling density depending on the density of the vacuolar content. The successful application of this technique opens the way to its use for double-immunolabelling.

Animals↗
PubMed1982

Antibodies to native type I and II collagens detected by an enzyme linked immunosorbent assay (ELISA) in rheumatoid arthritis and systemic lupus erythematosus.

Anti-native type I and/or type II collagen antibodies measured by an immunoenzymatic immunosorbent assay were found to be present in 22.2% of the rheumatoid arthritis cases studied, 27.5% of systematic lupus erythematosus patients and 28.6% of patients with Overlap Syndrome. These antibodies were not associated with any particular clinical or radiological form of rheumatoid arthritis. Nevertheless, antibodies to native type I and/or type II collagens were associated with seropositive rheumatoid arthritis and antibodies to native type II collagen with rheumatoid arthritis which have antinuclear antibodies. In addition antibodies to native type I collagen were especially present in rheumatoid arthritis with extra-articular symptoms. Antibodies to native collagens disappear or are diminished after corticosteroid treatment. Unlike gold, D-penicillamine or non-steroid anti-inflammatory drugs have no effect on levels of antibodies to native type I and II collagens.

Adrenal Cortex Hormones↗
PubMed1982

[Enzyme linked immunosorbent assay to detect antinucleic acid antibodies (anti-DNA and anti-RNA antibodies) (author's transl)].

The present paper describes a new ELISA type immunoenzymatic method for the detection of antibodies directed against nDNA, dDNA, and double stranded RNA (poly A, poly U and poly l, poly C). The antigens are adsorbed on glass beads but as some nucleic acids are difficult to adsorb on glass beads, polylysine was used to bind the antigens to the beads. The beads were saturated with sheep serum to get rid possible unspecific bindings. The sera are diluted before use with PBS containing sheep serum. The rate of antinucleic acid antibodies is obtained by subtraction of OD with antigen from OD without antigen. The technique was applied to human sera from patients with various autoimmune diseases (mostly disseminated lupus erythematosus). There is a rather good correlation between the determinations of anti-nDNA antibodies obtained with the ELISA and with indirect IF on Crithidia Luciliae.

Adult↗
PubMed1982

Location of FTS (facteur thymique sérique) in the thymus of normal and auto-immune mice.

This study was concerned with the in situ localization of facteur thymique sérique (FTS) by immunoelectron microscopy in the thymus of normal C57BL mice and aged auto-immune SWAN mice. Normal young mice have anti-FTS antibodies fixed specifically on the floccular material present in the cytoplasmic vacuoles of epithelial cortical and medullary cells. In aged auto-immune SWAN mice the anti-FTS antibodies show an activity only in the granules present in the vacuoles or free in the cytoplasm of epithelial cells. The floccular material is not labelled by the same antibodies. FTS positive granules show a repetitive structure which is characteristic of crystalline protein formations. The presence of FTS in the granules of cells confirm the hypothesis of FTS storage in the cytoplasm of epithelial cells in vivo during the auto-immune process.

Animals↗
PubMed1982

[Study of antihistone and anti-bicatenary RNA antibodies in connective tissue diseases using immunoenzyme technic].

Use of an immunoenzyme technique to seek two types of antibody (AB): antihistones and bicatenary anti-RNA, led to study of their distribution in different connective tissue and autoimmune diseases. Total antihistone AB and the H1 and H2b antifractions were seen almost exclusively in lupus (52.5 per cent of cases), principally during active phases of the disease since 87 per cent of active forms had antihistone AB as against 15 per cent of inactive forms. Antihistone AB would thus appear to be a valuable laboratory investigation in the diagnosis and surveillance of lupus. Bicatenary anti-RNA AB were more widely distributed, being found in DLE, RA, scleroderma and in various disorders with antinuclear antibodies. However they were slightly more common in active forms of lupus and their titres appeared to fall more quickly than those of antinuclear AB and anti-DNA AB at the time of clinical improvements. In RA, bicatenary anti-RNA AB were more commonly associated with inflammatory and progressive forms but further studies involving larger populations would be necessary to confirm these findings.

Arthritis, Rheumatoid↗
PubMed1981

Enzyme-linked immunosorbent assay for anti-histone antibodies and their presence in systemic lupus erythematosus sera.

We describe an enzyme-linked immunosorbent assay (ELISA) with adsorption of histones (total and fractions) on glass beads and saturation of excess sites with sheep serum. The anti-histone antibodies are detected with peroxidase conjugate and developed with Trinder's reagent which has great stability. This very sensitive method detects anti-histone antibodies in 53% of SLE patients and in virtually no other diseases. Positive reactions are observed only with total histones and fractions H1 and H2b.

Absorption↗
PubMed1980

Clinical and laboratory features of canine lupus syndromes.

Determination of the specificities of antinuclear antibodies in the sera of 20 dogs presenting with symptoms of a lupus-like syndrome permitted their separation into 2 groups. The first group of 14 dogs all had antibody activity to DNA-histone antigen(s), and 4 of them also had antibodies to native DNA (nDNA). The Farr test with standard buffer was found to be unsatisfactory for the measurement of anti-nDNA antibodies in dog sera due to a high incidence of false positive reactions; these could be eliminated by the inclusion of sodium dodecyl sulfate in the buffer system. The second group of 6 dogs was characterized by the presence of antibodies to extractable nuclear antigen. In every serum tested diseased dogs had a diminished level of circulating thymic factor as compared to controls of the same age, suggesting that a diminution of suppressor T cells may be an etiologic factor.

Animals↗
PubMed1980

Cytoplasmic localization of FTS (facteur thymique sérique) in thymic epithelial cells. An immunoelectronmicroscopical study.

This study was concerned with the localization of the facteur thymique sérique (FTS) by immunoelectronmicroscopy in cultured thymic epithelial cells. The FTS antibodies labelled differently sized cytoplasmic vacuoles containing a substance of variable density. This substance occurs occasionally in crystalline structures which reacted strongly with purified FTS antibodies. On the ultrastructural level the FTS-positive cells were demonstrated to fix specifically keratin antibodies and to contain tonofilaments. Some of these cells originated from the thymic medulla proved by the presence of microvilli-forming cytoplasmic vacuoles. The existence of FTS-positive crystalline inclusions confirmed the hypothesis of a storage process in the cytoplasm of thymic epithelial cells in vitro or in pathological conditions.

Animals↗
PubMed1980

Lupus-like syndrome in some strains of nude mice.

Nude mice (strains ORL, C57BL, BALB/C), as well as neonatally thymectomized mice, develop spontaneous antinuclear antibodies (ANab) and antidouble-stranded DNA antibodies (dsDNAab). Later, these animals develop a lupus-like syndrome in which immunoglobulin (Ig) deposits appear, first in the kidney glomeruli, then at the dermoepidermal junction and in the choroid plexus. In the beginning, these Ig are IgM and IgG2; later, IgG1 and IgA deposit in the kidneys. The classes of the Ig of the deposits correspond to those of the circulating ANab-Ig, except for IgA. Acid eluates from kidneys and skin, contain anti-DNA histone ab. An increased C1q binding activity is observed in 8- to 12-wk-old ORL nude mice with ANab, but is not observed in age-matched ORL nude mice without ANab. These data indicate the participation of ANab in the constitution of immune complexes and of tissular Ig deposits in nude mice. The antinuclear autoimmunization process, in nude as well as in thymectomized mice, might be interpreted as a defect of T suppressor cells, which normally control autoimmune clones. However, in contrast with the preceding strains of nude mice, nude mice from the IFFA centre, which have a different genetic background, develop neither ANab, nor dsDNAab, nor glomerular lesions. Three hypotheses can be proposed to explain these particularities of IFFA nude mice. They either have an immune response regulating system, independent of the thymus which is defective or absent in the other strains, or they are deprived of lymphocytes able to produce ANab and dsDNAab, or there are not enough free nuclear antigens to stimulate an immune response. Preliminary results obtained in our laboratory favour the first hypothesis.

Animals↗
PubMed1980

Characterization of facteur thymique sérique (FTS) in the thymus. I. Fixation of anti-FTS antibodies on thymic reticulo-epithelial cells.

Facteur thymique sérique (FTS) is a circulating nonapeptide inducing T cell differentiation. Its strict thymus dependency, already shown by its disappearance after thymectomy and its presence in thymus extracts, was confirmed using indirect immunofluorescence or immunoperoxidase by the binding to reticulo-epithelial cells of an antibody produced against synthetic FTS. The specificity of the reaction was demonstrated by the inhibition of binding observed after preincubating the anti-FTS antibody with synthetic FTS.

Animals↗
PubMed1979

[Absence of anti double stranded DNA antibodies in viral hepatitis (author's transl)].

The presence of anti double stranded DNA antibodies (ds DNA Ab) in the sera of patients with acute viral hepatitis and B virus chronic active hepatitis has recently been described. In contrast with these results we found no ds DNA ab, but anti single stranded DNA antibodies (ss DNA ab) in the sera from patients with these diseases. The ds DNA ab were studied simultaneously by two methods: Immunofluorescence (IF) on crithidia luciliae and Farr test. In this later case it was shown that the ds DNA preparation contained no ss DNA because it did not react with an anti ss DNA rabbit serum. Our results indicate that the above mentioned authors have been using in their Farr test a preparation of ds DNA contaminated by ss DNA, thus revealing the presence of anti ss DNA ab but not anti ds DNA ab, in the sera of their patients. On the other hand the presence of ds DNA ab has been shown in the sera of 85% of the patients with systemic lupus erythematosus and of 20,5% of the patients with non EB chronic active hepatitis. At least one part of these later are probably autoimmune diseases.

Acute Disease↗
PubMed1979

[Lymphocyte migration inhibition in rabbit experimental syphilis (author's transl)].

During rabbit experimental syphilis a suspension of Treponema pallidum (TP) was used as antigen. The evolution of anti TP antibodies was followed up to the fifteenth month, the antibody titer was maximum at month seven. A stimulation of leukocytes migration test was observed up to the sixth month then a significant inhibition appeared (chi square test) which persists after the ninth month. Hence the possibility was discussed that some form of cellular immunity appears secondary while immune response was mostly of humoral type at the beginning of the disease.

Animals↗