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J C Monier

Publications and source records attributed to J C Monier.

At least 73 records · Page 4Linked to original sources
PubMed1986

[Anti-fibronectin autoantibodies in systemic lupus erythematosus, rheumatoid polyarthritis, and various viral or bacterial infectious diseases].

A micro-enzyme linked immunosorbent assay (ELISA) aimed at detecting anti-fibronectin (anti-Fn) antibodies has been developed and standardized. Fifty sera from systemic lupus erythematosus (SLE), 50 from rheumatoid arthritis (RA) patients, as well as 200 sera from patients with bacterial or viral infections were assayed for the presence of anti-Fn autoantibodies. The IgG fractions of three representative positive sera (1 SLE, 1 RA and 1 streptococcal endocarditis) were digested with pepsin and the resulting F(ab')2 fragment assayed in the test. The presence of the anti-Fn activity in these fragments as well as lack of correlation in individual sera between the level of anti-Fn (as determined by ELISA) and that of Ig or immune complexes, suggest that our anti-Fn autoantibodies are indeed detected in our assay. The meaning of these antibodies, which were also found with bacterial and viral infections is discussed within the frame of the fibronectin biological properties.

Arthritis, Rheumatoid↗
PubMed1986

Demonstration of anti-mitochondria antibodies by ELISA. Comparison with the indirect immunofluorescence technique on organ sections.

We determined the optimal conditions for the detection of anti-mitochondria antibodies by an enzyme linked immunosorbent assay (ELISA). Pig heart mitochondria was used at 5 mg/ml to coat polystyrene microplates and a serum dilution of 1/200 to carry out testing. This procedure detects anti-M1, M2, M4 and M6 antibodies and is more sensitive than indirect immunofluorescence. Anti-endoplasmic reticulum and anti-ribosome antibodies give negative results but anti-ds-DNA at high titres are a possible cause of false positivity.

Animals↗
PubMed1986

Antihistone antibodies detected by ELISA and immunoblotting in systemic lupus erythematosus and rheumatoid arthritis.

Antihistone antibodies were sought in sera from patients with systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), melanomas, leukemias and other cancers (particularly breast cancer) by micro-ELISA, using a solution of total histones as antigen. This solution contained H1 and core histones (H2A, H2B, H3 and H4). ELISA was positive in 59.8% of SLE cases, 5.2% of RA cases, 11.1% of melanomas, 13.6% of leukemias and 5.6% of other cancers. Immunoblotting using total histones enabled us to clarify the histone fraction recognized by antihistone antibodies. In SLE, these were mainly anti-H1 and anti-H2B antibodies. In RA, antibodies recognized all histone fractions. However, some sera from patients with RA stained the H4 band more intensely.

Arthritis, Rheumatoid↗
PubMed1985

Antibodies to ribonucleic acids detected by ELISA in systemic lupus erythematosus, systemic sclerosis and rheumatoid arthritis.

The natural and synthetic antiribonucleic acid antibodies (anti-RNA) are more frequently found in systemic lupus erythematosus (SLE) (80%) than in systemic sclerosis (66%) or in rheumatoid arthritis (RA) (54.5%). SLE sera contain antibodies directed against a broader variety of RNA than systemic sclerosis sera and most RA sera. Fifty-two percent of SLE and 29.2% of systemic sclerosis sera recognized at least 5 of the 7 RNA types studied, whereas RA sera recognized only one or 2. Synthetic antipolynucleotide antibody activities are mainly antipolyriboguanylic-ribocytidylic acid (prG-rC), antipolyriboadenylic-ribouridylic acid (prA-rU) and polyriboadenylic acid (prA) antibody in SLE, antipolyriboguanylic acid (prG) and antipolyribocytidylic acid (prC) antibody in systemic sclerosis and anti-prG-rC, anti-prC antibody in RA.

Arthritis, Rheumatoid↗
PubMed1984

Non-specific binding of heat-aggregated IgG to histone detected by ELISA.

Anti-histone antibodies are currently detected by micro ELISA in systemic lupus erythematosus sera from humans, mice and dogs. Here we show that the control-heated sera may bind non-specifically to the whole histones and histone fractions. The heated immunoglobulins binding to histones are mainly IgG and to a lesser extent IgA, but never IgM. These false positive ELISA reactions occurred only with aggregated IgG which binds to histones via Fc; IgM rheumatoid factor prevented their fixation. Immune complexes do not seem to interfere significantly in the detection of anti-histone antibodies with the ELISA test.

Antibodies↗
PubMed1984

Specificities of antinuclear antibodies detected in dogs with systemic lupus erythematosus.

Five hundred and eighty dogs with at least one clinical sign compatible with a systemic lupus erythematosus (SLE) were entered in a prospective study aimed at evaluating the prevalence of antinuclear antibodies (ANAb). SLE was diagnosed in 38 of these dogs (group A) which fulfilled at least four American Rheumatism Association (ARA) criteria; of these, sixteen had ANAb titers greater than or equal to 4096. The 23 dogs which met three or two ARA criteria (group B) had an ANAb geometric mean titer (GMT) of 259. Dogs (group C) with only 1 criterium had an ANAb GMT of 75. Anti-ds-DNA Ab were present in 6 dogs from group A (16%), and 2 dogs from group B (9%). Anti-histone Ab were present among dogs from group A, B and C with frequencies of 81%, 67% and 26%, respectively. Among dogs from group A, the ANAb titers and the levels of anti-histone Ab correlated positively when individual sera were considered. Antibodies against the soluble nuclear antigen (SNA) were detected in 74%, 39% and 13% of the dogs from groups A, B and C, respectively. Antibodies initially described in human SLE also exist in SLE dogs. Anti-Sm Ab were found in 24% of dogs in group A. With anti-RNP Ab the frequency was still lower (10%). However, two other types of anti-SNA Ab against RNAse and trypsin-resistant antigens, not found in human "reference sera", were often detected. The first type (anti-type 1 Ab) was found in 26% and 9% of group A and group B, The first type (anti-type 1 Ab) was found in 26% and 9% of group A and group B, respectively; the second type (anti-type 2 Ab) is less frequent, and was found in 13% and 17% of group A and B, respectively. It appears that testing for anti-Sm, anti-type 1 and anti-histone Ab should be performed in order to improve the diagnosis of SLE in dogs.

Animals↗
PubMed1984

Generation of hybridoma antibodies to double-stranded DNA from non-autoimmune BALB/c strain: studies on anti-idiotype.

A hybridoma obtained between normal spleen cells from BALB/c mice (a non-autoimmune strain) and SP2-O-Ag 14 myeloma cell line was designated as HB2. These hybrid cells produced an IgM kappa-anti-ds-DNA antibody, but their specificity was limited to some polydeoxyribonucleotides such as natural ds-DNA from calf thymus, poly dG-poly dC, poly d(GC) and poly d(GC)-poly d(GC). In contrast, poly dA-poly dT, poly d(AT) were not recognized. The configuration of the nucleic acid helix plays a small role if any, in the building of the epitopes recognized by the hybridoma HB2 antibodies, while the presence of G and C appeared to be essential. These epitopes could not be found on ss- and ds-polyribonucleotides. B cells able to produce anti-ds-DNA antibodies are therefore present in non-autoimmune BALB/c mice, but not enough to produce the corresponding antibodies at a detectable level in the serum. Following immunization of BALB/c mice with hybridoma HB2 monoclonal antibodies, anti-idiotype antibodies were obtained which also recognized idiotopes present in the serum from both murine MRL/1 and human systemic lupus erythematosus (SLE).

Animals↗
PubMed1984

Localization of thymulin (FTS-Zn) in mouse thymus. Comparative data using monoclonal antibodies following different plastic embedding procedures.

The distribution of thymulin (FTS-Zn) was studied in thymuses from normal mice (OF1) or autoimmune mice (NZB). Thymulin localization was investigated using immunocytochemical techniques on sections of GMA and epon-embedded mouse thymuses. Two monoclonal antibodies were used: anti-synthetic thymulin and anti-intracellular thymulin. In the immunofluorescence assay, GMA sections allowed a more subtle localization of thymulin in the cytoplasm of epithelial cells (with a vacuolar pattern) compared to the epon sections (with a homogeneous fluorescence in the cytoplasm). In both cases, the number of labeled cells was greater in the medullary region than in the cortex of the thymus. At the electron microscopic level, immunolabeling of epon ultrathin sections showed ferritin distributed in some of the epithelial cell vacuoles. The two monoclonal antibodies revealed similar distributions of thymulin in the thymus. The results obtained in this study confirm that the amount of thymulin is greater in the epithelial cells of normal compared to autoimmune thymuses.

Animals↗
PubMed1984

Binding capacity of sera from systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) to C3c. Evaluation by enzyme linked immunosorbent assay (ELISA).

Immunoconglutinins (IKs) are autoantibodies directed against antigenic determinants on C3 complement component. An ELISA was performed to detect IKs in sera from 50 RA patients, 50 SLE patients and 50 normal subjects. Comparison showed significantly higher levels in patients than in normal subjects (p less than 0.001) and higher IKs levels in RA than in SLE (p less than 0.001). IKs were not related to others biological tests, except a statistically significant inverse correlation between IKs and circulating immune complexes levels detected by conglutinin binding assay in RA.

Adult↗
PubMed1983

Specific estrogen binding sites in human lymphoid cells and thymic cells.

The binding of estrogen in preparations of human peripheral blood mononuclear cells, as well as by splenic and thymic cells is demonstrated by three different approaches (Dextran-coated charcoal method, whole cell assay, and gel filtration on a sepharose 4B column). Scatchard's analysis of [3H]-moxestrol (R2858) and [3H]-estradiol binding proves the existence of a single class of receptor sites having a dissociation constant of 0.18-2.4 X 10(-9) M. Physicochemical properties of the binder, including binding capacity and steroid specificity, are quite similar to those reported for the thymus of small mammalian species or human thymoma.

Humans↗
PubMed1983

Sex-dependent systemic lupus erythematosus-like syndrome in (NZB X SJL)F1 mice.

(NZB X SJL)F1 (NS) mice were previously shown to develop sex-dependent thymic abnormalities in the course of aging. The possible occurrence in these mice of various autoimmune manifestations characteristic of a systemic lupus erythematosus (SLE)-like syndrome is investigated. Female NS mice died faster and exhibited antinuclear (AN), anti-ds-DNA antibodies and circulating immune complexes earlier in life and in greater amounts than male NS mice. At 12 months of age immunoglobulin deposits were detected in the renal glomeruli and at the dermo-epidermal junction of the skin. These deposits were more frequent and more intense in females than in males. In addition, proteinuria was found to rise with aging in females but not in males. These data demonstrate that NS mice suffer from SLE symptoms which, like the thymic abnormalities, are influenced by sex-related factors. The study of castrated males and females and of androgen-treated females suggests that androgens exert an inhibitory effect on these SLE symptoms. Preliminary genetic analysis further indicates a probable polygenic control of these SLE symptoms in NS females.

Aging↗
PubMed1983

Thymic abnormalities induced by castration in aged (NZB X SJL)F1 male mice: distinct effects of early and late castration.

Female but not male (NZB X SJL)F1 (NS) hybrid mice develop thymic abnormalities during aging. To determine the possible participation of androgens in this sex-related difference, we investigated the effect of androgen deprivation, as can be achieved by orchidectomy, on the cellular composition of the thymus of old NS males. Mice were orchidectomized at ages ranging from 3 weeks to 9 months and their thymuses were studied at the ages of 12 or 18 months. Phenotypic characterization of the intrathymic lymphocyte population was carried out using flow cytofluorometry analysis. Orchidectomy performed early in life (3 weeks-3 months) resulted, at 12 months of age, in thymic alterations (expansion of dull Thy-1+ cells, emergence of surface immunoglobulin-bearing cells) resembling those occurring spontaneously in old NS females. In contrast, when orchidectomy was performed later in life (6-9 months), there was a numerical increase of all thymocyte subsets but no major qualitative abnormality at either 12 or 18 months of age. Therefore, the absence of thymic disease in intact NS males may reflect primarily a suppressive effect of androgens that can be reversed by early but not by late orchidectomy.

Animals↗
PubMed1983

Effect of histamine on leukocyte migration tests in vertebrates. II. Study of the lack of species specificity of leukocyte inhibiting factor--production inhibitor (LIF-PI).

Immune regulatory effects of histamine are now well established. We have previously demonstrated that human T lymphocytes having histamine receptors, produce a soluble factor inhibiting the production of LIF. In this present paper we have shown that production and function of this factor is not species specific. Indeed the leukocyte inhibiting factor-production inhibitor (LIF-PI) produced by human, guinea pig or dog lymphocytes inhibits the production of LIF by human, guinea pig and murine lymphocytes to a similar extent. This property allows for easier testing of LIF-PI particularly from human subjects.

Animals↗
PubMed1983

Identification of canine T lymphocytes by membrane receptor to peanut agglutinin: T-lymphocyte identification in dogs with lupus-like syndrome.

Canine T lymphocytes were detected, using fluorescent peanut agglutinin (PNA) as a marker. Using a fluorescent technique and cytofluorometry, 70 +/- 11% and 72.4%, respectively, of peripheral blood lymphocytes were bound to PNA. Of thymocytes, 97 +/- 4.5% were detected by fluorescent PNA, but less than 1% were detected for lymphocytes from bone marrow. The T-lymphocyte depletion and enrichment indicated that PNA was bound to lymphocytes recognized by anti-T-lymphocyte heterologous serum. A T-lymphocyte deficiency was detected among 8 dogs with a lupus-like syndrome.

Animals↗
PubMed1983

Anti-tubulin antibodies in autoimmune thyroid disorders.

The presence of circulating antibodies directed against a cytoskeletal element, microtubules, in patients with autoimmune thyroid disorders, has been studied using pure brain tubulin as antigen. Immune complexes were immunoprecipitated using a goat anti-human immunoglobulin antibody. Twenty sera among 48 (41%) from patients with Graves' disease and nine sera among 16 (56%) from patients with Hashimoto's thyroiditis had increased levels of anti-tubulin antibodies as compared to that of 26 sera from control subjects. Only one serum among 11 from patients with toxic adenoma was positive. Very similar results were obtained using protein A adsorbent to collect immune complexes. Specificity of the tubulin binding activity was ascertained by dilution of the sera and displacement of tracer tubulin by unlabelled pure tubulin from rat or human brain. Anti-tubulin antibody titres were variable; one serum was positive at dilution higher than 1:15,000, a titre similar to those obtained in animals experimentally immunized against tubulin. Binding of labelled and unlabelled tubulin to immunoglobulins from positive sera was strictly competitive. The apparent affinity constant for the binding of tubulin to human anti-tubulin autoantibodies determined on four sera was 0.2-0.6 X 10(9)/M. There was no significant association between anti-tubulin antibodies and anti-microsomal antibodies or anti-thyroglobulin antibodies or thyroid stimulating antibodies. In contrast, only five to six per cent of sera from patients with other autoimmune diseases: lupus erythematosis or pernicious anaemia, had increased levels of anti-tubulin antibodies. In conclusion, tubulin represents a new autoantigen which is expressed rather specifically in autoimmune thyroid disorders and probably independently from the classical thyroid antigens.

Adult↗
PubMed1982

Histone antibodies in systemic lupus erythematosus. A possible diagnostic tool.

Antibodies to total histones and histone fractions H1, H2a-H4, H2b, and H3 were measured in serum samples from 61 patients with systemic lupus erythematosus (SLE), 33 with rheumatoid arthritis, 17 with systemic sclerosis, and 20 with various other diseases by use of a sensitive immunoenzymatic assay. Histone antibodies were present in 52.4% of the SLE samples whereas only 1 of the samples from other diseases was positive (systemic sclerosis). The presence of these antibodies in SLE patients was not associated with any specific clinical manifestations, but was correlated with activity of the disease: 87% (20 of 23) of patients with active SLE, in particular 9 of 9 not yet treated, showed histone antibody whereas only 18% (4 of 22) of samples from patients with inactive SLE were positive. We believe that the measurement of histone antibodies would be a useful addition to the present laboratory parameters (antinuclear and double-stranded DNA antibodies and circulating immune complexes) for the diagnosis and progression of systemic lupus erythematosus, particularly since they seem to appear during or just before the onset of an active phase and tend to be absent during remission.

Adolescent↗