Evidence for a fibroblast chalone.
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Biomedical subjects
Publications and source records attributed to J C Houck.
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A specific endogenous inhibitor for lymphocyte DNA synthesis that can be isolated from the lymphoid system and which is probably cell specific is described. The inhibitor is thermolabile, is destroyed by trypsin, and has a mass of about 30,000 to 50,000 daltons.
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Confluent monolayers of either mouse or diploid human fibroblasts contained no measurable amounts of either collagenolytic (pH 5.5) or proteolytic (pH 7.5) activities. Within a few hours after exposure of these cells to antiinflammatory drugs, significant amounts of these enzymatic activities were demonstrable extracellularly. These activities were profoundly decreased in cultures simultaneously treated with the anti-inflammatory drugs and cycloheximide or actinomycin D.
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