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Biomedical subjects

J C Houck

Publications and source records attributed to J C Houck.

At least 55 records · Page 3Linked to original sources
PubMed1975

Lymphocyte chalone concentrates and their effects upon leukemic cells in vitro.

Lymphocyte chalone is a cell-specific but not species-specific noncytotoxic and endogenous inhibitor of the transformation and mitotic activity of normal lymphocytes stimulated in vitro by either lectins or antigens. However, human- and mouse-established lymphoblastic cell lines were killed and their uptake of thymidine was inhibited after incubation for almost one complete mitotic cell cycle. The chalone lymphocytotoxicity effect was also demonstrated, using primary cultures of EL-4 lymphoma cells. Lymphocyte chalone was highly cytotoxic to leukemic cells under crowded condition (cells in G1-G0) in contrast to S phase chemotherapeutic agents. Finally, thymus chalone lymphocytotoxicity appears to be specific for T versus B lymphoblastic cell lines.

Animals↗
PubMed1975

Cytotoxic effects on splenic ultrafiltrates upon leukaemic lymphocytes.

Ultrafiltrates from spleen inhibited both DNA synthesis and the proliferation of normal lymphocytes stimulated inculture from both mouse and man without apparent cytotoxicity. However, the same doses of this spleen ultrafiltrate will kill up to two-thirds of the leukaemic lymphoblasts from both mouse and man after 24 h incubation. This unique lymphocytotoxic effect could also be demonstrated on fresh primary cultures of leukaemic lymphocytes and was highly effective on slowly growing established cell lines under crowd culture conditions. Furthermore. ultrafiltrated thymus extract did not affect the DNA synthesis rates of the viability of NC-37 lymphoblasts, which have B cell characteristic. Thymus extract was cytotoxic to Molt cells, which have T cell characteristics.

Animals↗
PubMed1975

The inhibition of macrophage migration inhibtory factor (38485).

Analogs of EACA were capable of inhibiting the in vitro activity of macrophage migration inhibitory factor (MIF). The most active analog of EACA in terms of MIF inhibition had four methyl groups separating an acetylated amino group from a free carboxyl group. This compound, acetylated 5-amino valeric acid, was capable of inhibiting completely MIF activity in vitro at a concentration of less than 10-6 M without demonstrating any appreciable antitryptic activity, even at 10-3 M. Antitryptically active EACA did not inhibit MIF except at very high concentrations (10-2M). Further, the most purified preparations of MIF obtained from aqueous extracts of thymus that we have studied contained no significant proteolytic activity upon denatured hemoglobin at pH 3-8. Therefore, we do not correlate anti-tryptic with anti-MIF activity in vitro.

Aminocaproates↗