Search PubMed⌕ Search

Biomedical subjects

J Browne

Publications and source records attributed to J Browne.

At least 55 records · Page 3Linked to original sources

Research directions in male sex work.

This paper provides a critical review of the literature relating to male sex work and outlines approaches to theorizing about and researching male sex work. The adequacy of these models to explain the male sex work industry is discussed. The literature reveals that earlier studies conceptualized male sex work as deviance and focused on the individual sex worker and his reasons for engaging in sex work. Although the research agenda has recently moved away from the individual sex worker towards the sex work industry, the focus of the investigation continues to be from a deviance rather than a work perspective. A number of aspects of male sex work have received little attention in the literature. These include the interpersonal dialogues and power relations that constitute the commercial sexual negotiation, the role of political and economic forces, and expressions of male sexuality within the practice of commercial sex. The paper suggests future research directions and argues that researchers need to draw on the strengths of the male sex work community in order to promote safe sex practices in commercial researchers need to draw on the strengths of the male sex work community in order to promote safe sex practices in commercial sexual settings.

Acquired Immunodeficiency Syndrome↗

Onchocerca volvulus: a comparative study of in vitro neutrophil killing of microfilariae and humoral responses in infected and endemic normals.

In vitro neutrophil-mediated microfilarial killing, humoral responses to crude adult worm antigens (OVAg) and to four recombinant Onchocerca antigens (GST-OV7, GST-OV103, GST-OV3.6, and MBP-OV16), as well as Western blot analysis to stage-specific Onchocerca proteins were studied in individuals from Bassa County, Liberia, infected with onchocerciasis and in endemic normals, defined as individuals without microfilaridermia. Both groups exhibited high levels of specific serum-dependent killing of microfilariae but could not be differentiated on the basis of these results. However, infected individuals had a significantly higher frequency of antibody response to OVAg (P = 0.0001) except GST-OV103. Based on the pattern of response to the different antigens, 17 categories of specific recognition were observed. Nine of these categories were common to both the infected individuals and the endemic normals, 2 were unique to the infected individuals, and 6 were unique to the endemic normals. Among the endemic normals, we identified a subcategory of individuals who had nondetectable levels of total IgG to OVAg by ELISA and had significantly lower IgG4 responses to OVAg. These same individuals demonstrated evidence of past infection, based on serum antibodies detectable by Western blot analysis to male and female adult worms and skin microfilariae, and had a positive response to two or less of the recombinant antigens. We believe that this subcategory may represent individuals with a unique status of immunity.

Adolescent↗

Preparation of antisera to recombinant, soluble N-acetylglucosaminyltransferase V and its visualization in situ.

N-Acetylglucosaminyltransferase V (GlcNAc-T V) is a glycosyltransferase which transfers N-acetylglucosamine in beta(1,6) linkage to the alpha(1,6)-linked mannose residue of Asn-linked oligosaccharides. This enzyme is characterized by several unusual properties: GlcNAc-T V is the largest lumenal Golgi glycosyltransferase described thus far, and its multiple mRNA transcripts range from 4.5 to about 9.5 kb; GlcNAc-T V mRNA and activity are regulated by the src tyrosine kinase signalling pathway; in brain tissue, large levels of GlcNAc-T V mRNA are present, but only relatively low levels of catalytic activity can be detected; a lectin-resistant cell line, Lec4A, expresses active GlcNAc-T V which is mislocalized intracellularly. In addition, the cell surface oligosaccharide products of this enzyme have been hypothesized to regulate intercellular adhesion. In order to devise specific inhibitors of this enzyme it is necessary to understand its physical structure and how structural changes can influence its activity and localization. We have expressed milligram amounts of a soluble form of recombinant rat GlcNAc-T V, purified it from CHO cell-conditioned media, and used it to prepare specific antisera. This antisera binds selectively to GlcNAc-T V and has been used to visualize B-16 mouse melanoma cell GlcNAc-T V on immunoblots after SDS-PAGE. When the antisera was used in immunofluorescence microscopy experiments on permeabilized B-16 and baby hamster kidney cells, intense, specific staining was observed in intracellular structures which appear to correspond to the Golgi apparatus.

Animals↗

Anaesthesia for thyroplasty.

The purpose of this report is to describe the anaesthetic considerations for layngoplastic procedures. Thyroplasty is a procedure which restores the voice in unilateral vocal cord paralysis. The procedure employs an external approach via a window cut at the appropriate level in the thyroid ala. A wedge of silastic is inserted against the inner perichondrium, thereby displacing the vocal cord medially and permitting voice production. Correct placement of the implant is assessed by asking the patient to phonate; patient cooperation is therefore necessary at certain times during the procedure. We describe our management of a patient undergoing thyroplasty. The use of a benzodiazepine agonist-antagonist combination provided both optimal operating conditions and patient comfort.

Anesthesia↗

The social meanings behind male sex work: implications for sexual interactions.

This qualitative study explores the meanings of the commercial sexual encounter between male sex workers and their clients. The study highlights the various social meanings male sex workers attribute to having sex, their typologies of clients, the psychic contexts of male commercial sex, safer sex interactions, and how these issues inform sexual behaviour. The data shows that the meaning attached to the act of having sex is an important aspect of the way in which participants perceive their partners, conduct themselves during sexual encounters, and engage in safe sex practices. Clients are categorized by sex workers according to their perceptions of 'them', which include 'marrieds', 'easy trade', 'undesirables', 'sugar daddies' and 'heaven trade'. Different types of clients pose alternate levels of risk to the safe sex practices of sex workers. The sex worker's definition of commercial sex as work enables him to separate work and personal sex and define work sex as 'not real sex', in which safe sex practices symbolize both the degree of self that is shared and protective work equipment. It was also found that this sample of sex workers do not negotiate safe sex. Rather they use 'modes of interaction' which direct the encounter either towards safe sex, or they refuse to continue with the transaction. The interactive modes identified are 'natural', 'educative', 'challenge', 'other options' and 'walk-out'. These modes of interaction are effective strategies for ensuring safe sex, and can be used by the broader community to gain partner compliance in safe sex practices.

Adult↗

The relationship of fasting insulin levels to vascular risk factors in a general practice in Northern Ireland.

OBJECTIVE: To investigate the relationship between insulin and vascular risk factors in a healthy male population at high risk of ischaemic heart disease. DESIGN: Computer-generated random number selection of subjects. SETTING: A suburban general practice population (total practice population 4500) in Northern Ireland. SUBJECTS: Four hundred male subjects, aged 35-65 years, were randomly selected with 273 responding. INTERVENTION: At interview, each subject completed a questionnaire, had blood pressure measured and a 12-lead ECG recorded. The next morning, fasting blood samples were taken and a timed overnight urine collection for the albumin excretion rate was returned. RESULTS: To exclude the confounding effects of other variables on insulin concentrations, a healthy nonobese, nondiabetic, normotensive group with no history of ischaemic heart disease, no family history of diabetes and not taking drugs was identified (n = 120). Within this group there was a significant correlation between insulin and triglyceride (r = 0.30; P < 0.05), high-density lipoprotein (HDL) cholesterol (r = 0.24; P < 0.05) and glucose (r = 0.30; P < 0.05). A group with higher insulin levels (n = 22) were compared to a group with lower insulin levels (n = 22). Serum triglyceride was higher (1.29 +/- 0.1 vs. 1.00 +/- 0.08 mmol L-1; P < 0.05), HDL cholesterol was lower (1.26 +/- 0.06 vs. 1.50 +/- 0.09 mmol L-1; P < 0.05) and plasma glucose higher (5.2 +/- 0.1 vs. 4.9 +/- 0.1 mmol L-1; P < 0.05) in the group with higher insulin levels. CONCLUSIONS: There is a relationship between insulin and triglyceride, HDL cholesterol and glucose but not blood pressure, cholesterol or low-density-lipoprotein (LDL) cholesterol in a healthy population at high risk of ischaemic heart disease.

Adult↗

Onchocerca volvulus: in vitro cytotoxic effects of human neutrophils and serum on third-stage larvae.

The cytotoxic effects of neutrophils co-cultured with infective third-stage larvae (L3) and autologous serum from three groups of individuals: infected (INF), non-patent endemic normals (EN) and non-endemic controls (NEC), were compared using a MTT (3-(4,5 dimethylthiazol-2yl)-2,5 diphenyl tetrazolium bromide) reduction assay as a biochemical parameter of larval viability. The MTT assay permitted the detection of L3 which had suffered varying levels of necrosis. The observations from this study indicate that infected individuals and endemic normals are capable of mounting a specific immune response against the infective L3. This is based on our findings of i) significantly greater numbers of L3 showed necrosis in neutrophil cultures of the INF and EN compared to those from the NEC, ii) a superior ability of cultures from these individuals to inhibit the molting of L3 to fourth-stage larvae, iii) IgG antibodies reactive with the surface of L3, detected by immunofluorescence in the serum of the INF and the EN and iv) recognition of specific antigens in extracts of L3 detected by Western blot analysis. The specific immune response directed against L3 is probably a significant immunological defense mechanism used to limit infection in endemic areas.

Adolescent↗

Microalbuminuria does not predict cardiovascular disease in a normal general practice population.

Recent studies have suggested that microalbuminuria is relatively common (9.4%) in non-diabetic subjects and that it is an excellent marker for increased cardiovascular risk. In an attempt to assess the prevalence of microalbuminuria in Northern Ireland where there is a high incidence of coronary heart disease, we studied 400 males, age 35-65 years, chosen at random from a Belfast general practice. There was a 73% response rate (n = 273). Sixteen per cent of the population has ischaemic heart disease. Microalbuminuria was defined as an increased urinary albumin excretion rate of 20-200 ug min-1. Thirteen subjects (4.7%) had an albumin excretion rate of 20 ug min-1 or more. After exclusion of subjects with diabetes mellitus or renal diseases, the group with microalbuminuria (n = 8), was compared to those without microalbuminuria (n = 256). There was no significant difference in the incidence of ischaemic heart disease between the two groups, nor did the group with microalbuminuria have a more adverse profile of vascular risk factors, apart from serum triglyceride (1.8 +/- 0.2 v 1.3 +/- 0.0 mmol l-1, p < 0.05) and plasma glucose (5.5 +/- 0.3 v 5.1 +/- 0.3 mmol l-1, p < 0.05) levels. We conclude that in a general practice from an area at high risk of ischaemic heart disease, the prevalence of microalbuminuria was low. Contrary to previous reports, microalbuminuria was not helpful in predicting subjects at risk of ischaemic heart disease.

Adult↗

Asking effective questions: an important leadership role to support quality improvement.

Effective questions stimulate, guide, and empower employees to think critically about the improvement processes that they are involved in as team leaders or team members in their daily activities. Learn how questions must be carefully phrased in order to provide guidance and maintain the integrity of the team's ability to choose options and implement change.

Attitude↗

Neuroendocrine effects of intravenous clomipramine in depressed patients and healthy subjects.

OBJECTIVE: Neuroendocrine challenge paradigms have been used to asses serotonergic systems in depression, but limitations in the specificity of many of these tests have been noted. In this study, the neuroendocrine responses to acute intravenous administration of the serotonin (5-HT) reuptake inhibitor clomipramine were assessed in depressed patients and matched control subjects. METHODS: Thirty hospitalized patients who met DSM-III-R criteria for major depression, and 30 healthy control subjects who were matched for age, sex, and season of year for the time of study, received 12.5 mg of intravenously administered clomipramine. RESULTS: The depressed patients demonstrated significant blunting of prolactin responses to clomipramine, as well as trends toward blunted ACTH and cortisol responses. There was no difference between the patient and control groups in growth hormone responses, plasma clomipramine levels, or self-reports of side effects. CONCLUSIONS: These data support the hypothesis that depressed patients have abnormal neuroendocrine responses to the intravenous administration of the 5-HT reuptake inhibitor clomipramine. Further study is required to delineate the mechanisms responsible for the abnormal response to intravenously administered clomipramine in depression.

Adolescent↗

Onchocerca volvulus: in vitro killing of microfilaria by neutrophils and eosinophils from experimentally infected chimpanzees.

Peripheral blood leukocytes from chimpanzees experimentally infected with third stage larvae, or with nodular microfilaria (mf) of Onchocerca volvulus, were evaluated for their ability to kill nodular mf in vitro. Inoculation of chimpanzees with third stage larvae, and prospective follow up, permitted each animal to be classified as patent, infected but not patent, or post-patent. Neither lymphocytes nor monocytes significantly adhered to, immobilized, or killed mf. However, neutrophils or eosinophils killed mf effectively in the presence of immune serum. Neutrophil and eosinophil enriched cell preparations from control animals were able to kill mf with serum from infected animals, but to a significantly lesser degree than when both cells and serum were taken from infected animals. Chimpanzees inoculated with mf showed a progressive increase in cellular killing of mf after a second inoculation which surpassed that seen with animals inoculated with third stage larvae. Antibody-mediated killing of mf by neutrophils and eosinophils may be important in the termination of patency in this infection.

Animals↗

The use of clonidine in the treatment of neuroleptic-induced tardive dyskinesia.

A double-blind crossover comparison of clonidine and placebo was conducted in seven subjects afflicted with neuroleptic-induced tardive dyskinesia (TD). Subjects received either clonidine, 0.4 mg/day, or placebo for a period of 8 weeks and were then crossed over to the opposing treatment modality. The effects of clonidine on TD symptomatology could not be distinguished from those of placebo (p greater than 0.05). However, comparison of prestudy ratings of TD severity to poststudy ratings revealed a significant reduction in TD symptoms (p less than 0.01). Close analysis of the data indicates that clonidine's effect on TD may have carried over into the placebo phase of the study, masking any true differences between clonidine and placebo treatment. Other factors which may have contributed to the lack of significant findings are discussed.

Adult↗

Characterization of recombinant monkey and human erythropoietin.

We have isolated the human EPO gene from a human genomic lambda library and a monkey EPO cDNA from an anemic monkey kidney cDNA library. These sequences have been expressed in both COS1 and Chinese hamster ovary cells. The expressed EPO has been shown to be fully biologically active both in vitro and in vivo and immunologically identical to the native hormone. In addition, administration of recombinant EPO to animals causes a dramatic increase in their hematocrit.

Anemia↗

Properties and relative immunogenicity of various preparations of recombinant DNA-derived hepatitis B surface antigen.

The gene for hepatitis B surface antigen was cloned into vectors for expression in yeast and mammalian cells. These hosts assemble the surface antigen protein into spherical structures containing cell-derived lipids. Particles were isolated from these cell cultures and purified by standard biochemical and biophysical means. Protein micells were formed from such particles by removal of lipid with nonionic detergent. Both particle and protein micellar preparations were formulated with alum adjuvant and tested for their immunopotency in mice. All the materials so analysed proved to be highly immunogenic. Safety and regulatory aspects of these materials and other potential and current hepatitis B vaccines are discussed. It is concluded that the yeast-derived materials and certain mammalian cell production systems present the most suitable opportunities for new hepatitis B vaccines.

Animals↗