Biomedical subjects

J Brock

Publications and source records attributed to J Brock.

At least 127 records · Page 7Linked to original sources

PubMed1984

[Age-dependence of adenosine deaminase activity in mouse lymphocytes from the spleen and thymus].

The ADA activity was determined in splenocytes and thymocytes of female CBA-mice in dependence on the age of the animals. The investigations were carried out in period of 0 to 120 weeks, the enzyme activities of corresponding cells of animals in the age of 6 weeks served as controls. Whereas the ADA activity of splenocytes did not change during the time of investigations (P = 5%; not significant in analysis of variance), the enzyme activity of thymocytes was diminished significantly in all animals older than 14 weeks. The ratio of the ADA-activities in splenocytes and thymocytes was 1:2 at the beginning of the investigations and 1:1 after 120 weeks.

Adenosine Deaminase↗

PubMed1984

Idiopathic retroperitoneal fibrosis. A sometime surgical problem.

The study was undertaken to focus attention on idiopathic retroperitoneal fibrosis, which may confront any surgeon operating in the abdomen or retroperitoneal area. Eleven patients, six men and five women varying in age from 35 to 76 years, were treated from 1969 to 1983. Two patients gave a history of methysergide and one gave a history of ergotamine ingestion. Two patients had associated aortic aneurysms and two had renal artery stenosis. Symptoms were related to entrapment of retroperitoneal structures, primarily the ureter, vena cava, gonadal veins, the aorta and its branches. Abdominal and costovertebral angle pain, testicular pain and swelling, and renovascular hypertension were the most common symptoms. The most common differential diagnostic problem was retroperitoneal tumor. Intravenous or retrograde pyelography were suggestive of the diagnosis in five patients, ultrasonography in two, and computerized axial tomography in another. Treatment consisted of ureterolysis and intraperitoneal transplantation or omental wrapping of the ureter in five, nephrostomy in two, renal-iliac arterial bypass graft in two, and renal autotransplantation in one. One patient was treated conservatively. Good results were achieved in eight, fair results in two, and one patient died postoperatively. Idiopathic retroperitoneal fibrosis should be kept in mind diagnostically in patients with unexplained abdominal pain and/or retroperitoneal lesions, and the surgeon prepared to employ appropriate operative measures for relief when it is encountered.

Adult↗

PubMed1984

[Secretory immunoglobulin A (S-IgA)/immunoglobulin A (IgA) ratio in cervico-vaginal lavage fluids].

Investigation of the immunoglobulins in the vaginal lavage fluids are semiquantitatively. The aim of the present study was to complete the semiquantitative S-IgA estimations with an "S-IgA/IgA" ratio. An antiserum against the secretory component (Anti-SC) and an S-IgA standard as well as an antiserum against the immunoglobulin A(Anti-IgA) and a serum IgA standard were used. The ratio was proved in vaginal lavage fluids from 5 fertile and 2 infertile women in the course of the menstrual cycle on days 8th, 11th, 14th, 18th and 24th. Progesterone, IgG and IgA were estimated simultaneously in serum samples. According to the "S-IgA/IgA" ratio patients were divided in 2 groups with values higher than 1 on the one side and with values lower than 1 on the other side. In cases with high "S-IgA/IgA" ratio an increased local antibody production and secretion is supposed.

Cervix Uteri↗

PubMed1983

[Specificity of cell-mediated cytotoxicity. II. Cytotoxic lymphocytes after in vitro allogeneic sensitization in the H-2 system].

Allogeneic intraperitoneal immunization in vivo leads to induction of cytotoxic lymphocytes. The cytotoxic reaction of these lymphocytes is specific in vitro against thymocytes as target cells and depends on identical private determinants of stimulator and target cell haplotype. Cross reactions are not observed in this model with H-2 incompatible haplotypes as target cells, also no autoreactivity is found. But if effector cells are used after stimulation in a mixed lymphocyte culture and mitogen stimulated spleen cells as target cells, cross reaction are generally found and autoreactivity too. Conditions of the trial determine the results. In vivo and in vitro induced effector cells destroy thymocytes as well as spleen cell blasts. The type of target cell does not influence the specificity of the cytotoxic reaction. Only in vivo induced effector cells react specifically.

Animals↗

PubMed1983

[Biochemical processes on membranes during lymphocyte stimulation].

A conformational change of the antigen receptors takes place with the help of macrophages, T helper cells and soluble factors after antigen binding. This conformational change may result in the following reactions: a) activation of a receptor-associated enzyme system b) influence on the lipid matrix and indirect activation of membrane-associated enzyme systems or influence on the cell metabolism by changing the ion flux c) activation of the cytoskeleton with following movements of the ligand-receptor-complexes and indirect activation of cytoskeleton-associated enzyme systems or influence on the metabolism by changing the ion flux. The adenylate cyclase (phosphorylating cascade) or the proteases (limited proteolysis of inactive proenzymes) may be the enzyme systems with regulatory effect which can be activated.

B-Lymphocytes↗

PubMed1983

[Secretory immunoglobulin A in amniotic fluid].

Secretory immunoglobulin A (S-IgA) was estimated in amniotic fluid samples by means of the single radial immunodiffusion according to Mancini. A monospecific antiserum against human secretory component was used. 163 amniotic fluid samples from normal pregnancies and risk pregnancies respectively were investigated. Within the 3rd trimenon the S-IgA content in amniotic fluid increased significantly. With respect to literature and examinations performed previously a connection between S-IgA content in amniotic fluid and fetal lung maturity seems to be possible.

Amniotic Fluid↗

PubMed1983

[Secretory immunoglobulin A (S-IgA) in amniotic fluid and in pharyngeal mucus and urine of newborn infants].

S-IgA was estimated in amniotic fluid and in mucus samples of pharyngeal cavities and urine samples of newborns for assistance of the hypothesis that there is a connection between S-IgA content in amniotic fluid and fetal pulmonary maturity. It was used an antiserum against human secretory component and an S-IgA standard in the single radial immunodiffusion according to Mancini and coworkers. High S-IgA concentrations in the mucus samples of pharyngeal cavities refer to fetal bronchial-pulmonary system as the probable essential part in the synthesis of S-IgA established in amniotic fluid of the 3rd trimenon in pregnancy.

Amniotic Fluid↗

PubMed1983

[Secretory immunoglobulin A in the amniotic fluid of healthy pregnant females].

Amniotic fluid levels of secretory immunoglobulin A (S-AgA) were measured by simple radial immunodiffusion according to the method of Mancini using a monospecific antiserum against the human secretory component. 256 samples from healthy pregnant women were examined. Amniotic fluid S-IgA concentration increases significantly during normal pregnancy and shows a loose correlation to the phospholipid level.

Amniotic Fluid↗

PubMed1983

[Quantitative determination of secretory immunoglobulin A in the serum of patients with gynecologic tumors].

Secretory immunoglobulin A (S-IgA) was estimated by mean of single radial immunodiffusion and an antiserum against secretory component as well as an S-IgA standard in serum samples from 28 patients with fibroid, 18 patients with uterine cervix cancer, 8 patients with endometrium cancer and 4 patients with cancer of the vulva. 21 patients formed a control group. The average values were mean = 37,7 +/- 10,6 mg/l (control group), mean = 34,6 +/- 16,2 mg/l (fibroid), mean = 43,4 +/- 18,6 mg/l (uterine cervix cancer), mean = 43,3 +/- 20,0 mg/l (endometrium cancer). There were not significant differences between tumour groups and the control group. Some increased S-IgA values in the several groups were striking in the course of which we have not found any connection between the increased S-IgA values and the tumour stages as well as the histological tumour types. Moreover it was proved whether the posttherapeutical S-IgA values were changed in consequence of surgery or radiotherapy compared with values obtained before therapy values. Significant alterations were not ascertained with the t-test for compared observations.

Adult↗

PubMed1982

[Chemotherapy schedule for the management of metastasizing gastric cancer. Methotrexate, adriamycin and 5-fluorouracil].

The possible effectiveness of methotrexate, 5-fluorouracil and adriamycin was tested in a phase I/II study of patients with metastasising gastric carcinoma. complete of partial remission occurred in 19 of 30 patients (approximately 63%). Two of 30 patients had complete remission. Median survival time of patients with remission cannot as yet be determined: about 68% are still alive after 17 months. Median survival time of patients with advanced tumour was only five months. The difference in survival curve is statistically significant (P less than 0.05). The study is continuing.

Adult↗

PubMed1982

Amniotic fluid secretory immunoglobulin A in normal pregnancy and in pregnancy complicated by rhesus isoimmunization.

Amniotic fluid levels of secretory immunoglobulin A (S-IgA) were measured by simple radial immunodiffusion according to the method of Mancini using a monospecific antiserum against the human secretory component. We examined 256 samples from healthy pregnant women and 149 samples from mothers suffering from rhesus isoimmunization. During normal pregnancy amniotic fluid S-IgA increased significantly and showed a loose correlation with the phospholipid levels. This was not observed in pregnancies complicated by rhesus isoimmunization.

Amniotic Fluid↗

PubMed1982

[Specificity of cell mediated cytotoxicity. I. Cytotoxic reactions in H-2 system after allogeneic sensitization in vivo].

Allogeneic sensitization in vivo results in cytotoxic lymphocytes specific in cytotoxic reactions against thymus cells as target cells. Only those targets are destroyed which have common private determinants with the stimulator cell. Target determinants on the K end of the histocompatibility complex are mainly responsible for cell lysis. Alloantibodies block specifically the cytotoxic reaction. First of all blocking activity is directed against the private determinant of the K end. Alloantibodies against public determinants from other H-2 incompatible combinations don't influence the cytotoxic reaction. The blocking antibodies belong mainly to the IgG class but antibodies of the IgM class show a inhibitory activity too.

Animals↗

PubMed1982

[Increase of the GvH reaction after alloimmunization. Influence of the serologically defined determinants of the H-2 system].

GvH reactions were induced in (CBA X C57B1)F1 and (CBA X A)F1 mice with sensitized lymphocytes. Specific sensitization results in an increased GvH reaction, sensitization against third party cells does not influence the GvH reaction. Therefore serologically defined public antigens of the H-2 system play not role in the GvH reaction. The comparison of different numbers of specifically sensitized lymphocytes, lymphocytes sensitized against third party cells, and control lymphocytes for the induction of the GvH reaction leads to the same result.

Animals↗

PubMed1982

[Demonstration of immunoglobulins, glycoproteins and beta 2 microglobulin in ovarian cysts and cystic ovarian tumors].

Immunoglobulins G, M, and A as well as secretory immunoglobulin A (S-IgA), pregnancy-specific beta1-glycoprotein, pregnancy-associated alpha2-glycoprotein, and beta2-microglobulin were analysed in fluid samples which had been taken from ovarian tumours and ovarian cysts. High S-IgA values were established from mucinous tumours and from one endometrioid adenocarcinoma.

Adolescent↗

PubMed1982

Suppression of GVH reaction in F1 hybrid mice by foetal liver cells.

The effect of foetal liver cells on GVH reaction with the parental lymphocytes in F1 hybrid mice was studied. The GVH reaction was induced in newborn (CBA X C3H)F1 and in 10-day-old (CBA X C57BL/6)F1 hybrid mice. Foetal liver cells did not induce a GVH reaction. The GVH reaction of CBA spleen cells in (CBA X C3H)F1 hybrid mice was demonstrable, although weak. Foetal liver cells suppressed the GVH reaction, in dependence on cell concentration, in (CBA x C3H)F1 but not in (CBA X C57BL/6)F1 hybrid mice. The possibility to induce transplantation tolerance with foetal liver cells is discussed.

Animals↗

PubMed1982

[Estimation of secretory immunoglobulin A in serum of pregnant women (author's transl)].

Secretory immunoglobulin A (S-IgA) was measured by means of radial immunodiffusion, according to Mancini, with a self-made antiserum to the secretory component being used together with a S-IgA standard. S-IgA serum concentrations went up with significance in the course of pregnancy, which was probably attributable to increased production of secretory protein in the breasts in preparation for lactation.

Female↗

PubMed1982

[Detection of secretory immunoglobulin A in vaginal fluid of hysterectomised women].

Secretory immunoglobulin A (S-IgA) or the secretory component (SC) in vaginal fluid of hysterectomised patients were established by means of simple and double immunodiffusion, according to Mancini or Ouchterlony, using an S-IgA standard. Since S-IgA was recorded from vaginal fluid, general questions were raised for origin and transport mechanisms of this localised immunoglobulin.

Female↗

PubMed1982

[Detection of protein in cervicovaginal irrigation fluid with special reference to secretory immunoglobulin A].

Secretory immunoglobulin A is a major component of the local immune system of the female reproductive tract (S-IgA). Information on differences between S-IgA values in sexual maturity, on the one hand, and those recordable from postmenopausal women, on the other, is reported in this paper. Detection of S-IgA in the vaginal fluid of hysterectomised patients has given rise to the question for the existence of an immune system of the vagina proper. Cervicovaginal fluid rinsed from pregnant patients was examined, with comparison being made between concentrations of secretory A, G, and M immunoglobulin, pregnancy-associated alpha 2 glycoprotein, and pregnancy-specific beta 1 glycoprotein.

Cervix Uteri↗