Search PubMed⌕ Search

Biomedical subjects

J Brock

Publications and source records attributed to J Brock.

At least 109 records · Page 6Linked to original sources
PubMed1987

[Importance of secretory IgA in human body fluids. I. Development of an enzyme immunoassay for secretory IgA].

This paper describes an enzyme-immunoassay for the quantitative estimation of secretory IgA(S-IgA). As solid phase a monoclonal antibody against human secretory component was used, a polyclonal peroxidase labeled anti-IgA serum as conjugate. Thus, the method was specific for S-IgA. The test was done in microtiter plates, the upper limit was 0.5 ng S-IgA/ml. The standards of our test system ran in normal ranges, the coefficients of variation were 6.9% in the within-day-test and 12.2% in the day-to-day-test. The described test system is effective and sensitive. It is suitable for clinical use.

Antibodies, Monoclonal↗
PubMed1987

[Regulation of the immunoglobulin content of the mucous membranes of the uterus: effect of estradiol on immunoglobulin A content].

A was used for the animal experimental investigations standard castration mouse model. Vaginal smears indicated no cyclic changes 14 days after the removal of the ovaries. 12.5 micrograms estradiol benzoate have been injected intramuscularly in this time. 3 days later uterus lavage fluids and tissue homogenates were used for the IgA-test, single radial immunodiffusion. The standard was prepared from mouse colostrum because S-IgA is the dominant IgA in secretions. The results were expressed as U/l selected arbitrary. Our findings of significant increased IgA concentrations in uterus lavage fluid after estradiol stimulation and, in contrary, no changed IgA concentrations in homogenates support the hypothesis, that the secretory component synthesis in the epithelial cells of the uterus is controlled by estrogens.

Animals↗
PubMed1987

[Secretory immunoglobulin A (S-IgA) in the amniotic fluid supports the fetal maturity profile].

Amniotic fluid levels of secretory immunoglobulin A(S-IgA) were measured by single radial immunodiffusion according to the method of Mancini using a monospecific antiserum against the human secretory component. 114 amniotic fluid samples were examined. The S-IgA values showed a correlation (r = 0.49) with the phospholipid levels. Additionally, it was observed no correlation between Serum and amniotic fluid S-IgA concentrations estimated as parallel investigations in 20 patients. S-IgA values greater than or equal to 82 mg/l were used to diagnose a sufficient fetal lung maturity. False negative values of S-IgA are possible. In 19 cases of newborns without signs of respiratory distress syndrome amniotic fluid S-IgA estimations were performed up to 32 hours before the delivery. False negative phospholipid levels were estimated in 2 cases, false negative S-IgA levels in 4 cases. The measurement of S-IgA in amniotic fluid will serve as an additional parameter for the estimation of fetal lung maturity.

Amniocentesis↗
PubMed1987

[Behavior of some tumor markers (secretory immunoglobulin A, pregnancy-associated alpha 2-glycoprotein, circulating immune complexes in the serum of patients with breast tumors].

Concentrations of secretory immunoglobulin A (S-IgA), pregnancy associated alpha 2-glycoprotein (alpha 2-PAG) and circulating immune complexes (CIC) were estimated in serum samples of 20 blood donors, 14 patients with benign mamma tumors, 44 patients with mastopathies and 33 patients with breast cancer disease. The proteins were determined by means of the single radial immunodiffusion and the polyethylene glycol precipitation. S-IgA serum concentrations of the cancer group compared with those of the benign tumors, were increased significantly (benign tumors: mean = 40.27 mg/l; s = 15.61; carcinoma disease: mean = 78.87 mg/l; s = 72.80). alpha 2-PAG serum concentrations of the cancer-and benign tumor group as well as the mastopathy group were also increased significantly in comparison with the blood donor group (blood donors: mean = 143.75 mg/l; s = 198.50; benign tumors: mean = 295.85 mg/l; s = 232.64; mastopathies: mean = 190.86 mg/l; s = 177.77; carcinoma disease: mean = 192.52 mg/l; s = 172.88).

Adolescent↗
PubMed1987

[Detection of immunoglobulins in cervical secretions in an inflammatory adnexal process].

Single radial immunodiffusion test was used for estimations of IgG, M, A and secretory immunoglobulin A (S-IgA) in cervical secretions of patients with pelvic inflammations. Salpingo-oophoritis was diagnosed and staged by laparoscopy and an activity index aimed controlling the during therapy and decreased after therapy, but the decrease after therapy was significant for IgA only. Immunoglobulin values before and after therapy were as follows: IgG (mean = 0.63 g/l, s = 0.54, n = 32 and x = 0.24 g/l, s = 0.27, n = 7), IgM (mean = 0.028 g/l, s = 0.028, n = 33 and mean = 0.17 g/l, s = 0.023, n = 7), IgA (mean = 0.12 g/l, s = 0.09, n = 30 and mean = 0.04 g/l, s = 0.035, n = 7), S-IgA (mean = 0.77 g/l, s = 0.66 n = 30 and mean = 0.28 g/l, s = 0.41, n = 7). In no case we found an S-IgA deficiency. On the other side IgM could not be estimated in cervical secretions of 8 patients with long time applicated intrauterine device. Therefore IgM in cervical secretions may be a sign of acute ascending inflammations.

Adolescent↗
PubMed1986

[Comparative studies of Fc receptors for IgG on resting and activated mouse T lymphocytes using different methods].

Fc-receptors for IgG (Fc gamma R) on resting (i.e. freshly prepared) and mitogen (Con A) or alloantigen-activated mouse spleen T cells were compared using binding of different markers such as 125J-labelled immune complexes, 125J-labelled anti Fc gamma R monoclonal antibody, FITC-labelled aggr. IgG and sheep erythrocytes covered with specific antibody (EA rosetting). C3b receptors were detected by rosetting with sheep erythrocytes covered with antibody and complement (EAC rosetting). The electrophoretic mobility of the cells without or after binding of aggr. IgG was also tested. A number of differences between resting and activated T cells were found: After activation of T cells by mitogen or alloantigen, a proportion of Fc gamma R-positive cells increased two to four times. Fc gamma R number per Fc gamma R-positive cell seemed to be higher on activated then on resting cells. Fc gamma R-positive resting cells did not shed their Fc gamma R upon incubation at 4 degrees C followed by incubation at 37 degrees C, but Fc gamma R-positive activated cells shed a remarkable proportion of their Fc gamma R on the same conditions. Binding of aggr. IgG caused a decrease of electrophoretic mobility of activated but not resting cells. Fc gamma R-positive resting cells were also C3b receptor-positive, whereas Fc gamma R-positive activated cells had no detectable C3b receptors.

Animals↗
PubMed1986

[Inhibitory serum factors in MLR and in PHA-stimulation in sera from normal, allogenic hyperimmunized and transplantation tolerant mice].

Sera from normal, allogeneically hyperimmunized and transplantation tolerant mice show an inhibitory effect on MLR and PHA stimulation. After gel chromatography these inhibitory factors are preferably found in fraction III for MLR (MW 250-750 KD) and III and IV for PHA stimulation. These factors are not identical with the IL-2 inhibitor. A specific inhibitory component with a MW of about 1000 KD is found in sera from transplantation tolerant mice. This suppressor factor could have a synergistic function in induction of specificity and activity of suppressor cell.

Animals↗
PubMed1986

[Stress-induced changes in the spleen and thymus of the mouse after skin transplantation].

The influence of the unspecific stress factors at the distribution of the lymphocyte subpopulations in spleen and thymus was investigated in skin transplantation model of the mouse after operation. The parameters total lymphocyte content, electrophoretic distribution pattern, activity and isoenzyme pattern of the LDH were compared in allogenic, syngenic, and sham transplanted mice with untreated control animals. Stress reactions were observed in the electrophoretic distribution pattern of the spleen cells as an increase of the quickly moving fraction (S-fraction) still on the 14th day after transplantation. Such reactions as an increase of the S-fraction were provable in the thymus cells on the 3rd and 7th day. The influence of the operation stress was further shown in a changed isoenzyme pattern of the thymus cells LDH. The relative portions of the LDH 1 and LDH 2 were reduced still 10 days after transplantation with an increase of the LDH 5.

Animals↗
PubMed1986

Safe, rapid administration of cisplatin in the outpatient clinic.

The records of 147 cancer patients who received at least three courses of cisplatin-containing chemotherapy in the Arizona Cancer Center clinic between 1982 and 1985 were reviewed to determine the safety and tolerance of cisplatin administered in the outpatient setting. Cisplatin was administered at doses of 50-120 mg/m2 every 3-4 weeks. The drug was added to 400 ml of 10% mannitol, was brought up to 1-L volume with normal saline containing 3 g of magnesium sulfate, and was administered iv over 1 hour. An additional liter of normal saline was administered over approximately 1 hour in patients who received cisplatin doses of 70-120 mg/m2. Courses were interrupted if the serum creatinine was greater than 1.5 mg/dl just prior to the next scheduled dose. The median total doses of cisplatin per patient were 487, 595, and 683 mg/m2 and complete plus partial response rates were 64%, 71%, and 78% for those patients who received 50-60, 70-90, and 100-120 mg/m2/course, respectively. Cisplatin-containing therapy was tolerated without evidence of renal failure and with only moderate to severe emesis in 25% of the patients. Calculated creatinine clearances dropped only 4.9%, 13.9%, and 14.9% between Courses 1 and 6 in patients receiving cisplatin doses of 50-60, 70-90, and 100-120 mg/m2, respectively. We conclude that cisplatin doses of 50-120 mg/m2 can be administered safely and with acceptable tolerance in the outpatient setting.

Adult↗
PubMed1985

Study of eosinophilia in relation to serum IgE level and cell mediated immunity (CMI) to soluble egg antigen (SEA) in human schistosomiasis mansoni.

Eosinophil count, delayed intradermal test and lymphoblast transformation test (LTT) using SEA, have been used to study the correlation of eosinophilia with CMI in patients with schistosomiasis mansoni. Only a weak positive correlation was found between the eosinophil count and both in vivo and in vitro manifestations of CMI. When IgE serum level was correlated with eosinophil count, a positive correlation was only found in cases with hepato-splenic disease (those with increased CMI).

Antigens, Helminth↗
PubMed1985

Adoptive transfer of transplantation tolerance in the H-2 compatible mouse system CBA/C3H. Transfer of lymphocyte subpopulations into irradiated and non-irradiated mice.

Transfer of neonatally induced tolerance in the H-2 compatible CBA/C3H strain combination is possible with different efficiency by injection of adherent and non-adherent spleen cells, unseparated spleen cells and lymph node cells from C3H-tolerant CBA mice into sublethal irradiated CBA mice. The most efficient cell populations are adherent spleen cells and lymph node cells. Successful transfer of transplantation tolerance is not possible to non-irradiated mice. The adherent fraction of spleen cells and lymph node cells contains a suppressor cell population responsible for transplantation tolerance against non-H-2 antigens. The induced transplantation tolerance is not due to a chimaeric state of C3H-tolerant CBA mice.

Animals↗
PubMed1985

[The role of the cytoskeleton in lymphocyte stimulation].

The influence of the cytoskeleton active drugs Cytochalasin B and Colchicine on the 3H-thymidine and 14C-uridine incorporation of Con A stimulated lymphocytes of mice was studied. Both drugs had no influence on the entry of the Con A incubated lymphocytes into the G1-phase but inhibited significantly the entry into the S-phase of the cell cycle. Cytochalasin B and Colchicine differ in their effect on the release of charge changing lymphokines. Colchicine did not influence this release in contrast to Cytochalasin B. The results show that both Cytochalasin B and Colchicine suppress cellular processes which are necessary for going beyond the commitment point. A possible importance of the microfilaments for the release of lymphokines and of the microtubuli for the expression of lymphokine receptors is discussed.

Animals↗
PubMed1985

Qualitative and quantitative Con A binding to mouse lymphocytes, T cell enriched spleen cells and allogeneically stimulated lymphocytes.

The qualitative and quantitative Con A binding behaviour of mouse spleen cells and allogeneically stimulated lymphocytes was investigated by means of fluorescence-optical and cell-electrophoretical methods. Contrary to B lymphocytes and unstimulated cultured T lymphocytes, normal as well as allogeneically stimulated T cells show a decreased electrophoretic mobility after incubation with Con A. This decrease correlates with a higher affinity and specificity to Con A on the one side and with a functional signal of Con A on the other side. Incubation with Con A is a stimulating signal for normal T lymphocytes and a suppressive one for allogeneically stimulated lymphocytes. The 3H-thymidine incorporation of B lymphocytes and unstimulated T cells is not influenced by Con A in spite of Con A binding. The amount of bound molecules is not of decisive importance for the mitogenic cultivated properties of Con A.

Animals↗
PubMed1985

[Detection of secretory immunoglobulin A (S-IgA) and secretory component (SC) in cystic fluids from ovarian tumors and ovarian cysts].

Secretory immunoglobulin A (S-IgA) and secretory component (SC) were estimated by means of double radial immunodiffusion and single radial immunodiffusion in 22 cystic effusions from 22 patients with cystic ovarian tumours and ovarian cysts. For the quantitative estimations of S-IgA a S-IgA standard preparation was used. S-IgA and/or SC were demonstrable in all cases of the mucinous cystadenomas and also in the cystic effusions of an endometrioid adenocarcinoma and of a serous cystadenoma. The S-IgA values were estimated between 230 and 1330 mg/1. The estimation of S-IgA in cystic effusions is useful to support and extend the histological diagnosis of ovarian tumours.

Adenocarcinoma↗
PubMed1984

[Tumor markers (alpha 2-macroglobulin, secretory immunoglobulin A, pregnancy-associated alpha 2-glycoprotein) in the serum of patients with bronchial carcinoma].

Concentration of alpha 2-macroglobulin (AMG), pregnancy associated-alpha 2-glycoprotein (alpha 2-PAG, PZ) secretory immunoglobulin A (S-IgA), pregnancy specific-beta 1-glycoprotein (SP1) and immunoglobulin E (IgE) were estimated in serum samples of 88 patients with lung cancer of stages, II-IV. The proteins were determined by means of the single radial immunodiffusion and the rocket immunoelectrophoresis. AMG and S-IgA serum concentrations of the cancer group compared with those of the control group, were increased significantly. AMG (control group: means = 1,43 g/l; s = 0,999; tumor group: means = 4,37 g/l; s = 1,758); S-IgA (control group: means = 37 mg/l; s = 16,6; tumor group: means = 48 mg/l; s = 24,2). PZ was found only in 52% of the patients with lung cancer by means of the rocket immunoelectrophoresis. The difference between the tumour and the control group was not significant. IgE could be detected only in one case with lung cancer (IgE = 2950 IU/ml). It was not possible to detect SP1 by means of the single radial immunodiffusion in serum samples of lung cancer patients.

Adult↗