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J Braun

Publications and source records attributed to J Braun.

At least 523 records · Page 29Linked to original sources
PubMed1986

Luminometric assays of seven acute-phase proteins in minimal volumes of serum, plasma, sputum, and bronchioalveolar lavage.

We describe immunoluminometric assays for seven acute-phase proteins, which can be determined in minimal volumes of plasma, serum, sputum, and bronchioalveolar lavage. The theoretical volume of serum or plasma required to measure all seven analytes in duplicate is 130 nL, although in practice the smallest volume of sample was enough to fill a hematocrit tube (about 25 microL of blood), collected from neonates by the heel-prick method. The assays could be performed with 10 microL of sputum or with 100 microL of bronchioalveolar lavage. We measured alpha 1-antitrypsin, alpha 2-macroglobulin, alpha 1-acid glycoprotein, thyroxin-binding prealbumin, C-reactive protein, and total and secretory immunoglobulin A. The assays are rapid enough for all results to be returned to the ward on the same day and are suitable for monitoring neonatal sepsis. All coefficients of variation, derived from compound precision profiles, were less than 7% for clinically relevant analyte concentrations. Correlation with commercially available nephelometric assays was good.

C-Reactive Protein↗
PubMed1986

Radiology in war.

Explore the source record for details and available documents.

Abdominal Injuries↗
PubMed1985

Pharmacokinetic studies of high-dose metoclopramide with and without forced diuresis.

The pharmacokinetics of high-dose metoclopramide (10 mg/kg body wt. in five infusions of 2 mg/kg body wt. each) was studied in 11 patients (5 females, 6 males) in two groups: group A with and group B (consisting of five patients) without forced diuresis. When the drug was infused, forced diuresis had no influence on the pharmacokinetics of metoclopramide (serum level after the 1st infusion was 851 +/- 361 ng/ml in group A versus 840 +/- 348 ng/ml in group B; after the 5th infusion it was 2,005 +/- 588 ng/ml in group A versus 2,463 +/- 1,350 ng/ml in group B). There were significant differences in the 24-h serum levels (582 +/- 308 ng/ml in group A versus 379 +/- 170 ng/ml in group B; P less than 0.05) and in the elimination half life (8.5 +/- 2.6 h in group A versus 6.1 +/- 1.1 h in group B; P less than 0.05). The results demonstrate that the dosage regimen originally suggested by Gralla for cytostatic drugs, with forced diuresis for high-dose metoclopramide therapy, may also be applied, with no dosage reduction, with to other cytostatic drugs which do not require forced diuresis.

Adult↗
PubMed1985

Brain mass as a manifestation of very late relapse in nonendemic Burkitt's lymphoma.

A 4-year-old boy was diagnosed as suffering from Burkitt's lymphoma with abdominal and maxillary involvement. The child was from a nonendemic area for Burkitt's lymphoma, and no association with Epstein-Barr virus was found. Two years after the completion of therapy, (3.25 years later) the child developed a solitary brain tumor, which was completely excised and was diagnosed as Burkitt's lymphoma. No meningeal involvement was found. Very late relapse in nonendemic Burkitt's lymphoma is very rare, and its presentation as a solid brain mass without meningeal involvement has not been reported before.

Brain Neoplasms↗
PubMed1985

Pharmacokinetics of tocainide in patients with severe renal failure.

The plasma levels of tocainide have been followed after oral administration of 600 mg p.o. to 20 patients with renal failure due to various causes, and to 8 healthy controls. The peak plasma concentrations in the patients with pyelonephritis (3.80 micrograms/ml) and interstitial nephritis (3.74 micrograms/ml) but not in those with glomerulonephritis (3.17 micrograms/ml) differed from that in healthy volunteers (3.24 micrograms/ml). The renal clearance of tocainide was well correlated with the endogenous creatinine clearance and was dependent on urine pH. No difference in renal clearance was observed between the patients groups. It is suggested that the changes in plasma levels are a consequence of decreased renal clearance. Creatinine clearance was shown to be a poor estimator of tocainide clearance, which suggests that extrarenal clearance plays an important role in the handling of the drug in the body. The findings are used to suggest a safe dosage regimen.

Acute Kidney Injury↗
PubMed1985

Improved fluorescent compounds for tracing cell lineage.

In this note simple methods for the synthesis of several new fluorescent cell lineage tracers are described. These are fluorescent dextrans with average molecular weights of approximately 11 X 10(3), and with one or more fluorophore molecules covalently coupled to each dextran chain. These fluorescent dextrans are brighter than commercially obtainable products, and can be microinjected using either air-pressure injection or iontophoresis. They are long-lasting and have a uniform distribution in the cytoplasm of embryonic cells, clearly revealing very fine cell extensions such as cilia, axons, and filipodia. A method is also described for covalently attaching free amino groups to fluorescent dextran to make the tracers cofixable with cellular constituents by aldehyde treatment. Fluorescent dextran-amine tracers allow embryonic cell lineages to be studied in fixed, permeabilized, or sectioned embryos.

Amines↗
PubMed1985

[Sonographic detection of thromboses of the renal veins and vena cava in adenocarcinoma of the kidney].

Renal cell carcinoma invades the renal vein and the inferior vena cava in 5-33% and 5-10% of the cases, respectively. The ultrasonic findings of 102 consecutive patients with renal tubular carcinoma were compared with the intraoperative and pathohistological results. With ultrasonography the renal vein could be visualized in 81% on the right and in 54% on the left side. 20 patients showed a thrombosis of the renal vein. The main renal vein could be demonstrated in 10 of 13 cases, with a malignant thrombosis in 7 cases. Ultrasound could not visualize any of the 7 thrombotic small renal vein branches. 4 of 5 tumour thrombi in the inferior vena cava could be shown sonographically. The role of ultrasonography in comparison to phlebography and computed tomography in assessing the normal and pathological venous system of the kidney is described.

Adenocarcinoma↗
PubMed1985

[Radiotherapy of prostatic cancer: staging and radiotherapy planning with transrectal ultrasound].

Transrectal ultrasound of the prostate is a cheap and well-reproducible examination for the prostate and seminal vesicles. The local tumor extension of a prostatic carcinoma can be visualized. By documentation of the ultrasound image on a polaroid paper picture, the topometric section of the prostate can be obtained. This picture can be used for dosimetry in individual radiation therapy-planning. Computed tomography is performed for detection of enlarged regional lymph nodes and demonstration of the topographic anatomy of the prostate in the lower pelvis.

Humans↗
PubMed1985

Delayed onset of traumatic extradural hematoma.

During a 4 1/2-year period, seven patients with delayed onset of an extradural hematoma were seen among 80 consecutively treated cases of extradural hematoma for a frequency of 8.75%. The hematomas were insignificant or not present on initial computerized tomography (CT) scanning. Repeat CT scans within 24 hours of admission showed sizeable hemorrhages. Six hematomas were evacuated, and one was reabsorbed spontaneously. In only one patient did neurological deterioration herald the onset of the extradural hematoma, four patients remained unchanged, and two improved before diagnosis. Intracranial pressure (ICP) was monitored in five patients, four of whom showed intermittent rise in pressure despite preventive treatment. Intracranial hypotension and rapid recovery from peripheral vascular collapse seemed to be contributory factors in the delayed onset of an extradural hematoma. Awareness of this entity, a high degree of vigilance, ICP monitoring, and repeat CT scanning within 24 hours of injury are strongly recommended in these cases, especially after decompression by either surgical or medical means, recovery from shock, or whenever there is evidence of even minimal bleeding under a skull fracture on the initial CT scan.

Adult↗
PubMed1985

Autoregulation of B cell growth by an immunoglobulin M autoantibody.

This study documents the autoregulation of B cell growth by an IgM autoantibody. This autoantibody is secreted by B lymphocytes upon stimulation by polyclonal activators and is identified by its potent inhibition of B cell growth induced by these agents (endotoxin, Fc fragment of human gamma-globulin, Mycoplasma bovirhinis, and anti-mouse Ig). The autoantibody specifically affects B cells: there was no effect on mitogen- or antigen-induced T cell proliferation and of responses to interleukin 1, interleukin 2, or interferon. Nonspecific effects were excluded by the ineffectiveness of serum and myeloma IgM. Also, IgG-IgM immune complexes were excluded. The binding specificity of the IgM autoantibody is not yet defined but appears to be a B cell surface structure distinct from membrane Ig. The autoantibody constitutes a ubiquitous, autoregulatory influence on B cell growth, which may be an important component in physiologic and pathologic states of B cell homeostasis.

Animals↗
PubMed1985

[The possibility of technical error in ultrasound studies].

Ultrasonography provides a diagnostic tool for patient examination by means of an imaging procedure according to sonic principles. An artificial image is created electronically from the different velocity of sound and intensity of sonic echos in various tissues. This echogram demonstrates an indirect image of the organs in the body, which differ in their echogenic structures. Three main causes for errors result from this principle: by the examiner, by the sonic principle and physical-technical limits of the ultrasound apparatus. These errors are presented with sonographic pictures and their causes are outlined.

Diagnosis, Differential↗
PubMed1985

Systolic time intervals before and after haemodialysis.

We investigated changes of haemodynamics during haemodialysis in 107 patients by noninvasive measurement of systolic time intervals. Heart rate and preejection period index increased and systolic and diastolic blood pressure, left ventricular ejection time and isovolumic contraction time decreased. We found, that if the pre-dialysis ejection fraction (EF) was more than 55 per cent, post-dialysis EF decreased by about 10 per cent. However if pre-dialysis EF was less than 55 per cent, post-dialysis EF increased by five per cent. Regarding cardio-thoracic-ratio, Sokolow-Lyon-Index (SLI) and repolarisation disorders we found the most remarkable decrease in EF in patients with normal sized hearts with enlarged SLI and repolarisation disorders.

Adult↗
PubMed1985

[Serum myoglobin following intermittent administration of succinylcholine and the effect of dantrolene. Clinical studies of children in halothane anesthesia].

Serum myoglobin concentration was observed over a period of 30 min following succinylcholine (SCH) in 30 children. They were uniformly anaesthetized with halothane; differences, however, concerned premedication and relaxation. They received in group 1 (n = 12) dantrolene 2 X 1 mg X kg-1 orally and SCH 2 X 1 mg X kg-1 i.v., in group 2 (n = 12) only SCH 2 X 1 mg X kg-1 i.v., and in group 3 (n = 6) neither dantrolene nor succinylcholine were used. The mean initial myoglobin concentrations were nearly identical (16.8-17.0 ng X ml-1). In the children without SCH (group 3) no changes could be observed; following SCH (group 2) the mean concentration increased to 1,610.5 ng X ml-1 after 30 min; additional premedication with dantrolene (group 1) led to a significant reduction of the myoglobin concentration (413.7 ng X ml-1; p = 0.031).

Anesthesia↗
PubMed1985

[Pathophysiologic role of the internal anal sphincter in chronic anal fissure].

Internal anal sphincter manometric and myoelectrical investigations were performed under basal conditions and in response to rectal distension in 17 patients with chronic anal fissures and 15 controls. Measurement of sphincter pressures were carried out by pull-through technique, using water perfused open-tip-catheters. Electrical signals were obtained employing concentric needle electrodes inserted into the internal anal sphincter. No statistically different resting pressures were noted between patients with fissures and controls. Just so no significant difference were found in frequency and amplitude of slow potentials generated by the internal and sphincter. Neither amplitude or frequency correlate with anal sphincter pressures. In both groups, transient rectal distension produced relaxation of the internal sphincter and were associated with inhibition or irregularity of electric activity. Distribution and amplitude of internal overshoot contraction showed no difference in both groups. It can be concluded that internal sphincter spasm can not be considered as the sole cause for persistence of fissures.

Adult↗