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Biomedical subjects

J Bouda

Publications and source records attributed to J Bouda.

At least 37 records · Page 2Linked to original sources
PubMed1989

[The effect of subclinical ketosis in dry cows on the composition of the colostrum and on health indicators in newborn calves].

Subclinical ketosis of dry cows was studied as to its effect on the composition of colostrum and on the health condition and selected clinico-biochemical parameters in the new-born calves of these cows. The experimental group consisted of eight cows with ketonuria and their calves. The control group consisted of six cows without ketonuria and their new-born calves. Calves of both groups were reared in similar conditions. Colostrum samples were taken from the first milking and then from the milking 24 hours after parturition. The blood of the calves was sampled before the intake of colostrum and 24 hours after birth. The concentration of ketone bodies, particularly beta-hydroxybutyrate, was much higher in the colostrum of both groups of cows than in the blood (62.6 mg total ketone bodies per 1 litre of the first colostrum of the cows of the experimental group). The concentration of ketone bodies slightly increased after parturition. The cows with ketonuria secreted into colostrum a larger amount of oxidated ketone bodies. Colostrum quality was good in both groups. Decreased viability was not recorded during the clinical examination of the calves after birth. The level of total ketone bodies in the blood of pre-colostral calves in the experimental group was 10.4 mg per litre (a much lower concentration than in blood of their dams), and exhibited no appreciable change after taking in the colostrum. The blood of calves born to cows with ketonuria contained significantly higher amounts of oxidated ketone bodies. No correlation was demonstrated between the contents of ketone bodies in the blood of cows, in the blood of calves, and in the colostrum. The calves of the experimental group were found to have a significantly higher AST activity in plasma, as compared with the control group. Twenty-four hours after birth, the quantity of immunoglobulins in the blood serum was significantly lower in the calves of the experimental group. Thanks to good attendance and hygiene, the morbidity did not increase and no losses occurred.

Acidosis↗
PubMed1989

[Diagnostic reliability of pre-biopsy methods (colposcopy and cytology) in predicting the histological basis of cervical lesions].

The more frequent incidence of cancer of the uterine cervix and thus of precanceroses in younger age groups, the introduction of new therapeutic methods (cryotherapy, CO2 laser, modernized diathermocoagulation) call for an estimate of the histological basis of the cervical lesion and in precanceroses assessment of its grade. These data must be provided by prebioptic methods (cytology and colposcopy). This diagnosis is essential to provide optimum therapy under the given circumstances. During the two-year period of 1985-1986 at the Gynaecological Clinic in Plzen 308 women detected by cytological screening were examined who were subsequently operated on account of a cervical lesion. In the investigated group a prebioptic predicition of high reliability was made. A concordant finding was recorded in 286 women, i.e. 92.9%. False negative, i.e. so-called risk errors were made in 9 women, i.e. 2.9%. False positive errors without risk, where the only consequence was unnecessarily radical treatment, were made in 13, i.e. in 4.2% women.

Adult↗
PubMed1987

[Trypsin inhibitor activity in the colostrum of cows].

The activity of the colostral trypsin inhibitor (CTI) was studied in seven milkings of colostrum in the course of the first five days after calving in 15 cows. The activity of the total trypsin inhibitor in the colostrum was ascertained in the course of the first two days after calving in ten cows in their second to fourth lactations and also in the colostrum of the first milking after calving in the case of 11 cows. The greatest CTI activity was found in the colostrum of the first milking after calving (301 micrograms X ml-1). Between the CTI activity and the concentration of total protein (CB) in the whey of the colostrum from the first and second day after calving there was found to be a statistically significant correlation (r = +0.929). The activity of total trypsin inhibitor was also highest in the colostrum from the first milking (499 micrograms X ml-1). After calving the CTI and the total trypsin inhibitor activities in the colostrum gradually dropped. Between the activity of the total trypsin inhibitor and the CB concentration in whey from the first, third, fifth and seventh milkings after birth a statistically significant correlation was found (r = +0.972). Statistically significant correlations were also found between the activity of the total trypsin inhibitor, the specific gravity of colostrum, the concentration of total protein and gamma-globulins in the whey of the colostrum from the first milking after calving. In metabolic alkalosis the concentration of total protein in the whey and the activity of trypsin inhibitor were found to be considerably lower than the average values.

Animals↗
PubMed1986

[The effect of the use of milk-based feed mixtures and whole milk on the development of selected indicators in the blood plasma in calves].

Two milk feeding systems were investigated as influencing the health and development of calves. After the termination of colostrum feeding, the ten animals of the experimental group were given whole milk whereas the control group (also ten calves) was given the Laktosan milk replacer. By the age of three months, blood was collected from the calves for biochemical examination in weekly intervals, later once a month. The content of urea, determined in the blood plasma of the calves of the experimental group was significantly lower in the fourth to seventh week. The plasma levels of nitrogen, potassium, calcium and magnesium were about the same in the experimental and control groups, being within the limits of the reference values. At the age of six to nine weeks, the content of inorganic phosphorus in the blood plasma of the tested animals was statistically significantly higher. Vitamin A concentration in the blood plasma was about the same in both groups. The content of vitamin E in the blood plasma of the calves of the experimental group was statistically significantly higher in the fourth to eight week of age. No significant differences between the two groups were observed in the plasmatic activities of aspartate aminotransferase (AST) and gamma-glutamyl transferase (GMT). The activities of alkaline phosphatase (ALP) were significantly higher in the third to fifth week of life. From the fifth to eighteenth week of age, the average daily weight gains were significantly higher in the calves given whole milk.

Age Factors↗
PubMed1986

[Utilization of urea nitrogen in young calves].

The influence of an increasing intake of urea in feed rations for calves on its levels in blood plasma and urine, development of health condition, weight gains and on other biochemical characteristics in the blood of calves was studied. The experimental group (n = 4) received urea in feed ration from the 17th day of age, at the beginning in the preparation Syrur, later on (from the 66th day) in the synthetic form only. The feed ration of the control group (n = 3) differed only in the zero content of urea. Over the period of ten weeks the blood was sampled for biochemical examination twice a week, since the 52nd day of age urine was also taken at the same intervals. Starting the 24th day of age, the levels of urea in the blood plasma of experimental animals were significantly higher (P less than 0.05). The concentrations of urea in the urine of the animals belonging to the experimental group were also higher, however, the differences in the average values were not significant. In both groups of animals under study no significant differences in the content of vitamin A, concentration of total protein, glucose, bilirubin, activities of aspartate aminotransferase (AST), gammaglutamyl transferase (GMT) and lactate dehydrogenase (LD) were observed. The average daily weight gains were higher in the calves of the control group, however, the differences were not significant. Over the whole experimental period the health condition of both groups of calves was good.

Age Factors↗
PubMed1985

[Selected indicators of colostrum quality in cows over the period of a year].

Under the conditions of large-scale breeding, seasonal changes in the total protein of colostrum whey, gammaglobulins, vitamins A, E, C, and carotene in cow's colostrum from the first milking, were studied in the course of the first 12 months. As indicated by the results of an analysis of 302 colostrum samples, the concentrations of total protein in whey were significantly higher in winter (P less than 0.01) than in the summer season. On the other hand, vitamin C levels were higher in summer than in winter. A lower level of total protein of colostral serum than the required minimum of 120 g per litre was recorded in 64.4% of the cows. A highly positive correlation (r = +0.981) was demonstrated between the concentration of total protein and gammaglobulins in colostrum whey. This fact makes it possible to consider and use for diagnostic purposes the total protein of whey as a sufficiently exact parameter of colostrum quality without having to determine the gammaglobulin level. On the basis of these analyses it is possible to some extent not only to evaluate the health condition of high-pregnant cows and the quality of attendance, but also to prognosticate the health of their calves.

Animals↗