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Biomedical subjects

J Bernard

Publications and source records attributed to J Bernard.

At least 271 records · Page 15Linked to original sources
PubMed1980

Bone marrow transplantation in Fanconi anaemia.

Five patients with Fanconi anaemia have been treated by bone marrow transplantation from HLA identical donors. Only one patient survived for more than 3 years. She is now perfectly healthy with complete haematological reconstitution with chimaerism and disparition of chromosomal abnormalities. In contrast, four patients died of acute severe GVHD soon after grafting. In addition, all had signs of severe cyclophosphamide toxicity. This evolution could be explained by a special sensitivity of FA cells to alkylating agents and may indicate the need to modify the conditioning regimen in FA patients.

Anemia, Aplastic↗
PubMed1980

Drosophila X virus RNA polymerase: tentative model for in vitro replication of the double-stranded virion RNA.

An RNA polymerase activity was found to be associated with the infectious drosophila X virus particles extracted from infected flies. The rate of synthesis was at first linear as a function of time, and then a plateau was reached. During the linear phase of the synthesis, the template and product were associated as replicative intermediates which were larger than the double-stranded RNA of the drosophila X virus genome, but the final product of the reaction was indistinguishable from the RNA genome with respect to its density, sedimentation coefficient, electrophoretic mobility, and RNase resistance. The results indicated that both strands of the genomic RNA were copied. The implications of these findings with regard to other virion polymerases are discussed.

Animals↗
PubMed1980

Viral hemorrhagic septicemia of rainbow trout: selection of a thermoresistant virus variant and comparison of polypeptide synthesis with the wild-type virus strain.

Serial passage of viral hemorrhagic septicemia virus at gradually increasing temperature selected for a variant virus that replicates at 25 degrees C and has a low pathogenicity for rainbow trout. Viral hemorrhagic septicemia virus-specific polypeptide synthesis was examined in epithelioma papulosum cyprini cells infected with either a wild-type strain or a thermoresistant variant. The wild-type N and M1 proteins were synthesized throughout the course of infection, whereas L, G, and M2 were more actively translated later in the replication cycle. The wild-type strain was more cytotoxic at 25 than at 14 degrees C despite the fact that no translation could be evidenced when the temperature was raised. When epithelioma papulosum cyprini cells were infected with the variant virus, the kinetic study was obstructed since protein synthesis was difficult to observe by the pulse method at a low multiplicity of infection and aborted when the multiplicity of infection was raised. The variant was less cytotoxic at 25 degrees C than wild-type virus.

Animals↗
PubMed1980

[Burkitt's tumor].

Explore the source record for details and available documents.

Burkitt Lymphoma↗
PubMed1980

Acute monoblastic leukemia: a clinical and biologic study of 74 cases.

Seventy-four cases of pure acute monoblastic leukemia (AMol) have been retrospectively studied. All patients were treated at Hospital Saint-Louis between 1970 and 1978. Diagnosis was based on morphological and cytochemical features according to the FAB classification. This type of leukemia occurred at any age and in both sexes, with a high frequency of extramedullary involvements. Hyperleukocytosis was very frequent and was significantly correlated with increased blood and urine levels of lysozyme, with renal failure and hypokalemia, and with coagulation abnormalities. AMol still has a poor prognosis, despite a best remission rate (75%) obtained with rubidazone, since the duration of complete remission was short. Central nervous irradiation prolonged remission and prevented meningeal relapses, while 6 meningeal relapses occurred in the patients not irradiated. The high frequency of the extramedullary relapses, including gum and skin, emphasized the question of persistant blast cell sanctuaries after achievement of bone marrow remissions. A more intensive induction with several drugs active against monoblasts could be more efficient and prolong the duration of complete remissions.

Adolescent↗
PubMed1980

[Acute leukemia in childhood: present status of 100 cases after 7 years of complete remission (author's transl)].

Amongst 1,200 leukemie children treated between 1958 and 1971, 60 are in complete remission for more than 10 years and 100 for more than 7 years. There were 96 acute lymphoid and 4 acute myeloid leukemias. Ten patients who have relapsed in the past have not done so lately. The F/M sex ratio is 1.5. Poor prognostic features were initially absent in 2/3 of cases. In 1/3 there was associated hyperleucocytosis and/or tumours. 93 children are in remission, their treatment having been stopped for 1 to 12 years. Five children relapsed and 4 are in a second remission for more than 2 years. Two children died in remission: one from a hepatocarcinoma and one from cardiac failure. These patients have been shown to have the following: 1) normal growth; 2) normal puberty: 8 patients have been able to reproduce, giving 10 children, one with multiple malformations; 3) school achievement and later socioprofessional behaviour has been normal. The patients have often sought a medical or paramedical career. Sequelae are minimal, psychological problems being minimal in the child. With the protocols used, mean remission curve shows a plateau after 9 years and complete definitive care is achieved in 92 per cent of patients surviving at 7 years. The very distant future outlook is not known. No other malignant haematological disease has occurred but one child died from a carcinoma.

Adolescent↗
PubMed1980

Application of the study of prognostic factors to the treatment of childhood (less than 20 years old) acute lymphoblastic leukemia.

405 children with acute lymphoblastic leukemia were stratified according to age, initial leucocytes count, lymph nodes, liver and spleen size, into three prognostic classes I, II, III. Protocol 08 LA 74 which they were applied included: 1)initial randomization between Prednisone, Vincristine, Daunorubicin or the same plus Cyclophosphamide for induction and reinductions; 2)doses adjustments to prognostic factors, increased doses being given to increased risk patients; 3)comparison between intrathecal Methotrexate and intrathecal Methotrexate plus Ara-C in addition to skull irradiation for CNS prophylaxis; 4)L-Asparaginase consolidation for all patients; 5)maintenance by 6-Mercaptopurine and Methotrexate in all patients and reinductions. The most striking conclusions to date are the improvement for increased risk patients, the frequency of primary testicular relapses contrasting with the low rate of meningitis, the prognostic implication of sex, the influence on remission duration of the number of courses necessary to achieve complete remission, the importance of using Cox Method to improve the identification of prognostic groups.

Adolescent↗
PubMed1980

[Complete remission of acute leukemia for more than seven years. A report on 100 children (author's transl)].

A total of 1200 children were treated for leukemia between 1958 and 1971, and 100 of these cases have had an uninterrupted remission for more than seven years: 10 children who relapsed at an early stage have had no further relapses; 93 are alive and in remission after discontinuation of treatment for between 1 and 12 years. Five of the children relapsed and four of these are in remission again. Two children died, one from a hepatocarcinoma and the other after cardiac failure of late onset. A study of these children showed that: 1 - growth rate was normal, 2 - puberty occurred at a normal age, and procreation was possible as 10 children were born from parents who had recovered after having leukemia, 3 - school-work and subsequent social and occupational behaviour were satisfactory, 4 - late psychological repercussions were absent or mild. Long-term prognosis appears to be good, and no other blood disorder has been reported in those cases that have recovered from the leukemia.

Acute Disease↗
PubMed1980

[Comparative study of the in vitro- and vivo- antimicrobial activity of seven antiseptic solutions for use on surgeon's hands (author's transl)].

The antibacterial activity of 7 antiseptic solutions has been studied both "in vitro en in vivo". The results obtained in both case were necessary and complementary since some products (e. g. Solubacter) show an irregular activity either "in vitro or in vivo" but show specific actions which can explain those result. Others (e. g. Betadine scrub), very active in vitro react slowly in vivo. The 3 solutions that have been shown to be the most antibacterial in both cases are 90 degrees alcohol. Remanol and Hibiscrub. Their efficacy has been shown to be fast, regular and relatively long lasting.

Anti-Infective Agents, Local↗