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J Bernard

Publications and source records attributed to J Bernard.

At least 199 records · Page 11Linked to original sources
PubMed1984

Non-Hodgkin's lymphoma occurring after Hodgkin's disease. Four new cases and a review of the literature.

This article describes four cases of non-Hodgkin's lymphomas occurring after successful treatment of Hodgkin's disease (HD). The clinical symptoms consisted of digestive disorders, and the histology confirmed an intestinal involvement in these four patients. In all cases patients had diffuse large cell types (intermediate or high grade). The respective role of HD treatment (combination chemotherapy in 3 of 4 patients with irradiation in 3 of 4 patients) and of other pathogenic hypotheses, are discussed.

Adolescent↗
PubMed1984

Cytochemical analysis of acid hydrolases expression during phorbol diester (TPA)-driven differentiation of B-chronic lymphocytic leukaemia cells in vitro.

Four acid hydrolases, acid phosphatase (AP), alpha-naphthyl acetate acid esterase (ANAE), beta-glucuronidase (BG) and N-acetyl-beta-glucosaminidase (NABG) were determined cytochemically in B-chronic lymphocytic leukaemia (B-CLL) cells exposed in vitro to the tumor promoter 12-0-tetradecanoyl phorbol 13 acetate (TPA). TPA, which has been previously shown to induce B-CLL cells to mature towards plasmacytoid cells, results in the progressive expression of the enzymes tested in the cytoplasm of malignant cells, in particular AP and ANAE. Furthermore, the sensitivity to inhibitors and the pattern of reactivity of ANAE provide evidence for an enzyme subtype normally restricted to plasma cells. Thus, acid hydrolases--some of which showing plasma cell type of activity--are expressed during B-CLL cells differentiation induced in vitro. These results confirm the value of cytochemistry in subtyping B-cell malignancies.

Acetylglucosaminidase↗
PubMed1984

Failure to distinguish ultrastructurally between T4+ (helper) and T8+ (suppressor/cytotoxic) T-cell subsets.

Human peripheral T-cell subpopulations revealed by monoclonal antibodies by means of a rosetting method were isolated by micromanipulation and submitted to electron microscopic analysis. The T3+ subset (total T cells) displayed a high degree of heterogeneity, including multiple transitional forms, from cells with a high nuclear to cytoplasmic ratio and rare organelles to cells with a low nuclear to cytoplasmic ratio and a complex system of cytoplasmic organelles. T4+ (inducer/helper) and T8+ (suppressor/cytotoxic) cell subpopulations were shown to have no evident distinguishing characteristics. They both displayed the same morphological variation mentioned for T3+ lymphocytes. On morphometric analysis, these two cell subsets were very similar, with only slight differences for cell surface roughness, volume of mitochondria, extent of nuclear indentation, and surface area of the rough endoplasmic reticulum. The significance of these minor morphological differences is discussed.

Animals↗
PubMed1984

[Not Available].

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Demography↗
PubMed1984

T-lymphocyte subsets in pleural fluids: discrimination according to traditional and monoclonal antibody-defined markers.

T-lymphocyte subpopulations in pleural fluid and in peripheral blood from 17 patients admitted for pleural effusion were identified by E-rosette formation and delineated by monoclonal antibodies OKT3 (peripheral T-cells), OKT4 (helper/inducer cells), and OKT8 (suppressor/cytotoxic cells). We studied 13 patients with specified pleural diseases (tuberculosis, malignancies, connective tissue diseases, congestive heart failure) and 4 patients with non-specified pleural diseases. Our findings showed that the percentage of T-cells increases in pleural fluid versus peripheral blood whatever the diagnosis is, and that these T-cells are predominantly helper/inducer cells. Moreover, a recently described T-lymphocyte subpopulation, which expresses neither T3 nor other monoclonal antibody-defined markers, seems to be concentrated in the pleural fluid, especially in tuberculosis and malignant effusions. Although T-lymphocyte delineation seems to fail to aid in etiological diagnosis of pleurisy, such determinations could provide informations about local pathogenic mechanisms.

Antibodies, Monoclonal↗
PubMed1983

[Recurrent familial hyperparathyroidism. A propos of 7 adenomas in 3 members of the same family. Review of the literature].

Over a period of 23 years, 3 members of a family of 5 presented with 7 parathyroid adenomas (4 in the first case, 2 in the second case, 1 in the third case). Excision of each adenoma, with systematic pre-operative assessment of the remaining parathyroid tissue, led to complete clinical and laboratory cure of each episode. The delay in the appearance of recurrence was between 3 and 9 years. After reviewing the literature, the authors stress the importance, in any case of hyperparathyroidism, of routinely investigating the serum calcium levels in members of the patient's family, especially if the patient is young and if he has had several episodes or multiglandular involvement in one episode. The authors discuss the literature concerning recurrent familial hyperparathyroidism with endocrine polyadenomatosis and the "hypercalcaemia-hypocalciuria" syndrome.

Adenoma↗
PubMed1983

Philadelphia chromosome-positive chronic myelocytic leukemia in children. Survival and prognostic factors.

The survival and the prognostic significance of the diagnostic characteristics of 39 children with Philadelphia chromosome-positive chronic myelocytic leukemia (Ph1-positive CML), seen between 1963-1976 at the Hôpital Saint-Louis of Paris, have been analyzed. The disease predominated in children older than age 4 years (95%), with girls being more affected than boys (24 versus 15). The clinical and hematological picture at presentation was similar to that observed in adults with Ph1-positive CML. Most children of this series were treated with busulfan which, as in adults, led to reduction of leucocytosis and organomegaly but did not prevent the occurrence of blastic crisis. Well-documented blastic crisis was observed in 78% of cases. Of 39 children, 12 were still alive, all in the chronic phase. Twenty-seven have died, 21 of them after blastic crisis, 4-156 months after diagnosis (median survival, 53 months). The effect of each diagnostic characteristic on survival was evaluated using the log-rank test. Of the 14 characteristics studied, only the degree of blood and marrow blastosis was associated with a shorter survival. Age, sex, bleeding, lymphadenopathy, hepatomegaly, degree of splenomegaly, hemoglobin level, total leucocyte, immature granulocyte (promyelocytes + myelocytes + metamyelocytes), eosinophil, basophil, and platelet counts in the peripheral blood were of no prognostic significance. The failure to attain a level of statistical significance for some characteristics found to be of prognostic value for adults, could be due to the small sample size and/or to the disease homogeneity. The results of this study, however, stress the importance of the initial blastic infiltration in determining the duration of survival, which is ultimately determined by the occurrence of terminal acute leukemia. In conclusion, this study shows that the Ph1-positive CML of childhood exhibits the same course, incidence of blastic crisis, and survival as the disease of adults. It also indicates that treatment with moderate chemotherapy, such as busulfan, has no effect on the duration of survival. Therefore, new therapeutic approaches are urgently needed for the treatment of this disorder in children.

Adolescent↗
PubMed1983

Phenotypic diversity within clones of human normal T cells.

Human normal T cells were selected for in vitro cloning according to the expression of T4, T8 or T10 antigens on individual cells. Clones were produced from each of these cells irrespective of the antigenic phenotype of the parental cell. The cloned progeny manifested, in many cases, shifts in antigen expression. Thus, T4+T8- cells gave clones expressing predominantly T4-T8+ and vice versa. The clonal expression of T4 and T8 seemed to be mutually exclusive. Antigenic shifts were recorded also in clones derived from T4-T8-T10- cells, resulting in T10+ clones which were also either T4+ or T8+ and from T4+T8-T10+ cloned cells yielding clones of either T4+ or T8+ cells. Testing functional properties we found that NK activity was mediated not only by T10+ cells but also, in some cases, by T4+ and T8+ cells. Moreover, TCGF production, which may reflect helper activity, was mediated not only by T4+ cells. Only the cytotoxic (CTL) activity seems to be confined to the T8 phenotype. Thus, it appears that T antigens, which seemed to be molecular markers of differentiation, are not markers for terminal differentiation and do not always reflect defined functional properties. These conclusions are drawn from cloning of normal T cells which manifest properties different from those of T-cell lines or T hybridomas.

Antibodies, Monoclonal↗
PubMed1983

Cytochemical analysis of human peripheral blood lymphocyte subsets defined by monoclonal antibodies.

Human blood lymphocyte subpopulations, revealed by a panel of commercially available monoclonal antibodies by means of a rosetting technique, were submitted to direct cytochemical analysis and were shown to have distinguishing characteristics. T cells reactive with OKT3 antibody (T3+) displayed higher beta-glucuronidase and dot-like alpha-naphthyl acetate acid esterase (ANAE) activity than T3- cells. Helper/inducer cells (T4+) were characterized by a high level of dot-like ANAE activity, whereas cytotoxic/suppressor cells (T8+) displayed selective naphthol-ASD-chloroacetate esterase activity. These results provide evidence for association of some cytoplasmic enzyme activities with the expression of membrane differentiation markers defined by monoclonal antibodies.

Acid Phosphatase↗
PubMed1983

Viral hemorrhagic septicemia of rainbow trout: relation between the G polypeptide and antibody production in protection of the fish after infection with the F25 attenuated variant.

A nonpathogenic variant of viral hemorrhagic septicemia virus has been selected which immunizes fish against a subsequent challenge with the wild-type virus strain. In this paper, we demonstrate that both the variant and the wild-type virus strains multiplied in spleen and kidney of infected fish, but the virus yield was lower for the variant and soon dropped below the sensitivity of our titration technique, which indicates that an early mechanism prevents the establishment of septicemia. This early mechanism could also be responsible for early protection since fish were already immunized 48 h postinfection with the variant. In a second step antibodies relayed that first defence mechanism to ensure long-lasting immunity. Antisera collected after immunization by the variant or the wild-type virus strain cross-reacted poorly in neutralization tests, which is in agreement with results obtained with plant lectins and proves that the variant is modified in its antigenic properties.

Agglutination↗