Morphological stability in Tetrahymena pyriformis.
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Biomedical subjects
Publications and source records attributed to J Berger.
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The results of interdepartmental determinations can be clearly presented in the laboratory in two ways: 1. Entry on a test card, according to Shewhart, as used in internal quality control. 2. Entry on a test card, constructed on the principle of the Cusum test. By the latter procedure, systematic errors are detected sooner than with the usual test card. A graphic variant of the Cusum test is described; a V-mask is not required; the card superficially resembles the usual control card, and the calculation time is minimal. This method may also be used to advantage in internal quality control.
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A microscopy test that used the typical shape of Mycoplasma pneumoniae cells growing on glass was investigated for its value for diagnostic purposes. Suspensions from 108 throat swabs were infected artificially with 102, 103, and 104 colony-forming units of three M. pneumoniae strains per ml. Agar medium, a diphasic medium, and the microscopy method with liquid medium in cover slip chambers were compared for isolation of the mycoplasmas. The mycoplasms were detected first by the microscopy method in nearly all concentrations tested. Typical M. pneumoniae cells could often be detected after 48 h. No differences were found between a laboratory strain and two low-passage strains. The experimental results suggest that under special circumstances the microscopy method could be a useful tool for isolation and identification of M. pneumoniae.
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A method is described which allows a quality control for demonstrating mycobacteria in the diangosis of tuberculosis. It does not test the differentiating procedures, but the manner of culturing and concentrating the bacteria by applying a statistical evaluation of serial experiments, thus allowing the statistically proven determination of the bacterial count. For this an evaluation method given by Cavalli-Storza was employed.
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This report gives a survey of the statistical evaluation in the combined study of "The course of gravidity and development of the child" in connection with toxoplasma infection which is sponsored by the German Research Council (DFG). In respect to the collected data it is possible to summarize as follows: 1. Neither the anamnestic nor the data of the observed gravidities allow the construction of a relationship between toxoplasma infection of a mother and the child after eliminating disturbing factors, especially that of the age (Tabelle 1-4 and Fig. 1). 2. An effect of prophylactic sulphonamide treatment in cases of latent and acute infected women has never been observed. The low number of abortions in the group of treated women is misleading because pregnant women with early abortion never received any treatment, therefore these people are included under the untreated group. 3. Up till now we have not observed a case of connatal toxoplasma infection in nearly 5,000 new born babies. After eliminating unreliable reports, abnormal developments which are thought to be related to toxoplasma infections have never been observed.
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Using artificially infected sheep, the response of a laboratory strain of Haemonchus contortus, which had not been subjected to anthelmintics for three years, was compared with that exhibited by a parbendazole resistant field strain following treatment with each of seven anthelmintics, the laboratory strain proved fully susceptable to thiabendazole, parbendazole, cambendazole, mebendazole, fenbandazole, levamisole and haloxon, in contrast to the field strain which showed a moderate to marked resistance to all five benzimidazoles, but was fully susceptible to levamisole and haloxon.
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