Search PubMed⌕ Search

Biomedical subjects

J Barry

Publications and source records attributed to J Barry.

At least 127 records · Page 7Linked to original sources
PubMed1985

Total parathyroidectomy for posttransplantation hyperparathyroidism.

The acute and short-term clinical course of 19 subjects who underwent total parathyroidectomy and forearm implantation for persistent hyperparathyroidism following successful kidney transplantation (mean [+/- SD] time after transplant 43.7 +/- 29.5 months) is described. Their mean preoperative serum calcium level of 10.8 +/- 0.5 mg% decreased to a nadir of 7.9 +/- 0.9 mg%, 62.5 +/- 27.7 hr after the operation. The lowest serum ionized calcium (1.80 +/- 0.2 mEq/L) was recorded 57 +/- 49 hr postoperatively. After an average of five hospital days, the patients were discharged with a mean serum total calcium concentration of 8.3 +/- 1.0 mg%. Three months following the operation, the mean serum total calcium concentration was 9.5 +/- 0.6 mg%. With an average follow-up of 19 months (range 3-36 months) serum total calcium was 9.6 +/- 0.6 mg%, with only one subject requiring calcium supplementation. Total parathyroidectomy with forearm implantation was associated with normalization of serum-immunoreactive parathyroid hormone concentrations and maintenance of stable allograft function. Our experience suggest that this procedure is an effective modality with a predictable postoperative recovery of parathyroid function when used to treat persistent hyperparathyroidism in the long-term survivor of renal transplantation.

Adolescent↗
PubMed1985

[Neurosecretion and peptidergic synapses].

The notion of interneuronal neurosecretory synapses, proposed since 1954, was ignored until 1973; dating from this year the equivalent notion of peptidergic synapses becomes progressively recognized. The same holds true for the notion of intranervous transfer of neurosecretory substances, also proposed in 1954 and which (together with the former notion of interneuronal neurosecretory synapses) perfectly agrees with our present state of knowledge concerning peptidergic communications in the central nervous system.

Animals↗
PubMed1984

Reconstruction of the cervical spine following anterior vertebral body resection: a mechanical analysis of a canine experimental model.

In some quadriplegic patients whose acute spinal injury consists of a vertebral body fracture with bony retropulsion anteriorly and ligamentous damage posteriorly, it is appropriate to perform a decompression of the injured spinal cord by excision of the fractured body. Reconstruction of such a spine to achieve immediate and ultimate stability can be quite difficult. This is a report of mechanical testing using a canine in vitro experimental model to simulate different general types of reconstruction systems applicable to the spine destabilized as above. The results explain some of the previously noted failures of the traditional anterior strut grafting procedures. In addition, one of the reconstruction systems tested (anterior and posterior tension bands compressing an anterior strut graft into place) appeared to have sufficient immediate stability to eliminate the need for a halo-vest in the postoperative period. If such a reconstruction system were to prove clinically applicable, it would be quite beneficial to the quadriplegic patient in his rehabilitation.

Animals↗
PubMed1984

The use of methylmethacrylate cement as an instantaneous fusion mass in posterior cervical fusions: a canine in vivo experimental model.

The authors previously predicted the failure of posterior cervical fusions utilizing methylmethacrylate cement as an instantaneous "fusion" mass, based on research using an in vitro canine experimental model. This report describes the results of in vivo canine studies on the same subject. Three groups of dogs had application of a posterior C4-C5 20-gauge cerclage wire and autologous iliac crest bone graft; application of a posterior C4-C5 20-gauge cerclage wire and methylmethacrylate cement; or application of a C4-C5 20-gauge cerclage wire only. This group represented the control group. The dogs were allowed to live for 3 months postoperatively, at which time they were killed and their spine fusions studied radiologically, mechanically, and histologically. Five of the bone fusions united solidly radiologically. Their flexion stability was statistically superior to the others. Histologic studies confirmed solid union of the fusion mass to the underlying bone. Four of the six methylmethacrylate fusions demonstrated cerclage wire fracture and methacrylate-bone separation by the second postoperative month. At the time the dogs were killed, their flexion stability was statistically inferior to the bone fusions and tended to be inferior to the controls as well. Histologically, fibrous tissue was noted to have grown between the methacrylate "fusion" mass and the underlying bone. This work provides a mechanical explanation for the well-known success of the traditional bony fusion. It further supports our original prediction regarding the failure of methylmethacrylate "fusions."

Animals↗
PubMed1982

Unsuspected adnexal masses in renal transplant recipients.

Post-transplant sonograms in 74 female recipients less than 40 years old revealed unsuspected adnexal masses in 5 (7 per cent). Sonographically, these masses were difficult to distinguish from other post-transplant fluid collections, such as lymphoceles, urinomas or abscesses and, consequently, led to further diagnostic evaluations in 4 patients. Of the adnexal masses 4 were excised surgically: 2 were hemorrhagic ovarian cysts, 1 was a follicular ovarian cyst and 1 was a paraovarian cyst. A coexisting gynecologic mass should be included in the differential diagnosis of a perinephric mass in female transplant recipients. Pre-transplant sonograms to identify or exclude occult pelvic masses in women may be of benefit in simplifying the postoperative management and minimizing unnecessary diagnostic evaluation.

Adnexal Diseases↗
PubMed1981

Scintigraphic detection of intrapulmonary bleeding using technetium-99m sulfur colloid: concise communication.

Radionuclide imaging has been successfully used clinically to determine sites of gastrointestinal hemorrhage, but its use in hemoptysis has not been studied. A dog model of intrapulmonary hemorrhage was devised. Utilizing technetium sulfur colloid, at doses of 4 and 15 mCi, bleeding rates of 0.1--0.2 cc/min were detected. In some dogs, however, significantly higher bleeding into a large bronchus, which causes a diffuse distribution of the radionuclide.

Animals↗
PubMed1980

Immunofluorescence study of LRH-producing neurons in prosimians (Tupaia and Galago).

Reactive LRH neurons were characterized in prosimians (Tupaia and Galago) by immunofluorescence using rabbit immunesera against unconjugated synthetic LRH, or LRH conjugated with bovine serum albumin. These neurons, which vary individually in number in one species, are mainly concentrated in the rostral hypothalamus (medial preoptic area and anterior hypothalamic area) and in the lamina terminalis. In contrast to the simians and man, immunoreactive perikarya were not routinely found in the mediobasal hypothalamus of the prosimians investigated in the present study. Reactive axons of the hypothalamo-hypophyseal tract are more numerous and conspicuous in the retrochiasmatic area and in the postinfundibular eminence. They give rise to radiating collaterals ending mainly around the capillaries of the primary portal plexus of the median eminence and of the infundibular stem (where they are generally more numerous). Reactive axons of the preoptico-terminal tract, originating from the perikarya of the lamina terminalis, end around the capillaries of the vascular organ or below and between the ependymal cells lining its ventricular side. In Galago a small but very distinct tract of reactive axons runs under the optic chiasma, between the lamina terminalis and the ventral labium of the infundibulum. Very fine reactive extrahypothalamic axons were observed in the posterior part of the habenular ganglia, along the preamygdaloid portion of the stria terminalis and along the blood vessels of the parolfactory area.

Animals↗
PubMed1980

[Ultrastructural localization of gonadoliberin in the gonadotropic cells of rat. Study by immunocytochemistry after cryo-ultramicrotomy (author's transl)].

By using ultrastructural immunocytochemistry after cryoultramicrotomy, gonadoliberin (GnRH) was localized in the gonadotropic cells of the female rat anterior pituitary gland. The immunocytological (IC) reaction was observed: (1) in the dense matrix of the secretory granules; (2) in the cytoplasmic matrix; (3) in the nucleus, in euchromatin, in the neighbourhood of heterochromatin. GnRH was seldom observed on the plasmic membrane. The values of the maximal dilution of the antibody (MDA) still giving an IC reaction decreased in the cytoplasmic matrix, in the nucleus and in the secretory granules. These observations, when compared with the data reported by biochemists, suggest that GnRH might be internalized in the gonadotropic cells. However, it remains to be shown that GnRH detected by IC reaction is specifically bound at binding sites.

Animals↗
PubMed1979

Immunofluorescence study of the preoptico-terminal LRH tract in the female squirrel monkey during the estrous cycle.

Reactive LRH neurons of the preoptico-terminal trace were studied with rabbit antisera to unconjugated or conjugated synthetic LRH in normally cyclic female squirrel monkeys. The specific immunoreactive material present along the preoptic-terminal LRH tract showed significant modifications that were particularly distinct (1) at the level of the perikarya, and (2) in the axons and axonal endings situated around the short and long capillary loops of the lamina terminalis. Conspicuous variations were observed during the luteal phase (with distinct and fast refilling), and the early and mid-follicular phases (suggesting a pulsatile mode of release); minimum concentrations were attained during the late and peri-ovulatory phases. A comparison is made between these modifications and those observed along the hypothalamo-infundibular LRH tract in the same animals. The preoptico-terminal LRH tract is thought to be one of the possible mechanisms controlling the sensitivity of gonadotropic cells to LRH, and the synthesis and storage of gonadotropins before their mid-cycle discharge.

Animals↗
PubMed1979

[Effects of estradiol-7 alpha-butyric acid on hypothalamus cells].

When Rat uterus was incubated at 37 degrees with estradiol-7 alpha-butyric acid (OII-7 alpha-bu), no interference was observed with the intracellular estradiol receptors. In addition, OII-7 alpha-bu did not display estrogenic effect such as in vivo inhibition of LH secretion in Rat and in vitro increased activity of the enzyme ornithine-decarboxylase in the chick oviduct. Contrary to these negative findings, we have observed preoptic and septal cells in the guinea pig where micro-iontophoresis of OII-7 alpha-bu triggers changes of the electric activity within a second. We submit therefore, that this latter response is due to an interaction between the acid estrogen and the neuron membrane.

Animals↗