Biomedical subjects

J Barnouin

Publications and source records attributed to J Barnouin.

At least 37 records · Page 2Linked to original sources

PubMed1994

[How does one detect biological imbalance and health disorders in the dairy cow within the framework of ecopathological research?].

The methods of detection of health and biological imbalances in the dairy cow, which can be performed in ecopathological studies, are presented. Mastitis, metritis, liver disorders, nutritional imbalances, reproductive disorders, stress, specific diseases and non-specific inflammations are reviewed. The possible future evolutions are analyzed.

Animal Nutritional Physiological Phenomena↗

PubMed1994

[The ecopathologic approach to the study of the relationship between nutrition and health in the dairy cow].

An ecopathological approach for examining nutrition-induced health disorders in dairy herds in France is described. Bovine dairy herds were surveyed for 4 yr to detect nutritional risk factors that discriminated between high and low disease incidence rates for retained placenta, milk fever, calf perimortality, metritis, liver disorders and infertility. In the cow, preventive feeding helps avoid nitrogen overnutrition, and reduces the requirements of Ca in the late gestation period, gives a satisfactory balance in polyunsaturated fatty acids before calving. The absence of feedstuffs including toxic components and a feeding system with few feedstuffs and few diet changes are also beneficial factors.

Animal Nutritional Physiological Phenomena↗

PubMed1994

Farming practices associated with the 'udder infection' complex.

A total of 4,129 dairy cows from 47 dairy farms were submitted each year after calving to a milk sampling for bacteriological examination. Six criteria defined the udder infection complex: frequency of minor, major and rare pathogens; rate of clinical and subclinical mastitis; and index of gravity of mastitis. Forty-seven variables described practices and their associations with the type of farms (defined by the above 6 criteria) were studied. The associated variables were dipping practices, covering of the milking parlour, disinfection practices, housing cleanliness and milk production.

Animal Husbandry↗

PubMed1994

[Nutritional factors and mammary inflammatory infection in the dairy cow. Ecopathological approach during the peripartum period].

The relationships between nutrition and udder inflammation/infection were studied in the dairy cow using an ecopathological approach. The study ('enquête écopathologique Bretagne') was performed over 4 yr in French herds with high-lactating cows. A long distribution time of Italian ryegrass silage and a low quantity of dietary cereal-based concentrates in the first 2 months of lactation was linked with low milk leukocyte counts, probably due to a high dietary energetic level or to a polyunsaturated fatty acid imbalance and a subsequent decrease in the synthesis of leukotriene LTB4. In the late gestation period, a long distribution time of fresh English rye-grass, which induced high uraemia and a larger distribution of vitamins A, D and E, was related to lower frequencies of major pathogens in the milk.

Animal Feed↗

PubMed1994

[Ecopathological approach to nutritional, metabolic and health factors linked to the protein and butyrate level in milk during the peripartum phase of the dairy cow].

An ecopathological analysis of milk protein and fat contents in high lactating herds is described. In herds with a high milk protein content, cows had shorter dry periods and more cereal-based concentrates and fresh English rye-grass in the dry diet. In the early lactation period, these cows had a higher risk for milk fever, their body score was less decreased, and the duration of feeding with Italian rye-grass silage was lower. In herds with a high milk fat content, the cows had shorter dry periods, lower plasma magnesium levels, more dietary vitamins A,D and E before calving, a decreased risk for metritis, and calved less frequently in summer.

Animal Feed↗

PubMed1994

Worm population characteristics and pathological changes in lambs after a single or trickle infection with Teladorsagia circumcincta.

The regulation of the worm population and of its pathological effects was studied after a single or trickle infection with T. circumcincta. One hundred and twenty lambs, 60 Romanov and 60 Mérinos d'Arles, 3 months old, were distributed in four balanced groups: non-infected (G0), infected with 7000 L3 per animal once and slaughtered after 4 weeks (G14) or 8 weeks (G18), and infected 8 times and slaughtered after 8 weeks (G88). Parasitological, histological, haematological parameters and weight gains were recorded on each animal. Female and artificially nursed lambs had lower worm burdens and egg counts (epg) than males and naturally suckled lambs. No difference in parasitological parameters was seen between the two breeds, but Mérinos lambs infected once, had a higher increase in pepsinogen concentrations than Romanov lambs. In the infected animals, a significant proliferation of mast and eosinophil cells was observed in the abomasum wall. Serum pepsinogen concentrations were significantly higher 3 weeks p.i. and the weight gain was depressed during the first month p.i. The worm population was more numerous and younger in group G14 compared with G18 in which 24% of the worms had been expelled during the second month p.i. The female worms in G18 were longer and had more eggs in utero and higher egg output. After the trickle infection (G88) the take was reduced, female worms were longer with more eggs in vagina (pars ejectrix) and there was a higher variability in the number of eggs (compared with G18 data). The pepsinogen rise was smaller but no specific effect was seen on histological and haematological parameters.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1993

[Automated micro-determination by transfer analyzer of circulating alpha-amino nitrogen].

An automated method for the determination of plasma free amino groups is described. The analysis is performed on a discrete computerized analyzer after plasma deproteinization using trichloracetic acid. The method is based on the formation of a complex between terminal NH2 radicals and 2,4,6-trinitrobenzene sulfonate (TNBS). The method is accurate (coefficient of variation = 2%), linear over a range of 0.2-7 mM/l and requires small analytic volumes; it also correlates well in the bovine with results from standard liquid chromatography (r = 0.953). With this method a single operator can determine free amino group contents of 50 samples per h.

Amino Acids↗

PubMed1993

The effect of inhibition of prostaglandin F2 alpha synthesis on placental expulsion in the ewe.

Five ewes were injected with two doses of a nonsteroidal anti-inflammatory drug (NSAI), lysine acetyl salicylate, at birth of their first lamb and one hour later, and five others were injected once only, at birth of their first lamb. A control group of six animals was constituted. The times needed for fetal expulsion and placental release were recorded. The peripheral plasma PgF2 alpha (as PGFM) levels were measured prepartum during the seven last days of gestation, at parturition, then 1 h, 2 h and 12 h after lambing. The results were compared among and within treatment groups. They indicate that the physiological increase in peripheral PGFM levels starts two days before lambing and that the level peaks at lambing. The normal decrease after parturition is emphasized by NSAI injections as detected 1 h and 2 h posttreatment (p < 0.01). The NSAI drug is short-acting as revealed by the lower PGFM levels in twice-treated animals 2 h after birth compared to once treated animals and the similar low levels in all three groups 12 h after birth. The fetal membranes were expelled normally in all treated and nontreated animals, but the time needed for placental expulsion in ewes injected with two doses of NSAI was longer than in controls (p < 0.05). A negative correlation (p < 0.05) was found between plasma PGFM levels measured two hours after lambing and the time needed for fetal membrane expulsion. PgF2 alpha appears to have a role in placental release in the ewe.

Animals↗

PubMed1992

Circulating PgF2alpha and nutritional parameters at parturition in dairy cows with and without retained placenta: Relation to prepartum diet.

Reproductive and biochemical parameters were studied at parturition in multiparous single-carrying Holstein cows. These were compared in animals with (n=14) and without (n=40) a retained placenta and then according to 2 prepartum diets (corn silage/concentrate, n=44; grass silage/concentrate, n=10) in cows with and without retained placentas. Cows with retained placentas had a 4-day shorter gestation period and gave birth to 5 kg-lighter calves than cows without retained placentas. Furthermore, their plasma PGFM -PgF2alpha main metabolite- (3325 vs 5675 pg/ml; P<0.01) and glucose (79.2 vs 95.2 mg/100ml; P<0.05) levels were lower and their protein concentration was higher (85.7 vs 76.5 g/l; P<0.05) than those of cows without retained placentas. Retained placenta incidence in cows fed grass-silage was higher than in cows fed corn-silage (60% vs 18.2%; P<0.05). Cows with retained placentas and fed corn silage had shorter gestation lengths, gave birth to lighter calves, and had less circulating glucose at calving (77.6 vs 96.5 mg/100ml; P<0.05) than cows without retained placentas and fed the same forage. Cows with retained placentas and fed grass silage had less PGFM (2172 vs 4530 pg/ml; P<0.05) than cows without retained placentas and fed the same forage. Calving number, sex ratio and preceeding milk yield were not different between the two groups of cows whatever their prepartum diet. In the dairy cow, retained placenta could be due to a PgF2alpha or an energy deficiency at calving. Roles of the dietary polyunsaturated fatty acids toward the Pg synthesis and of the energy supply before calving in relation to retained placenta are discussed here.

Journal Article↗

PubMed1991

An aetiological hypothesis for the nutrition-induced association between retained placenta and milk fever in the dairy cow.

In the dairy cow, a statistical association between milk fever (MF) and placental retention (PR) has been observed in epidemiological studies. In order to prevent both diseases, an understanding of their common aetiological mechanism is necessary. In this paper, a new causal hypothesis concerning the nutrition-induced association between PR and MF has been put forward. This hypothesis integrates the aetiological concepts arising from the studies carried out by the authors of this paper, and those reviewed from the literature. The aetiological pathway leading to associated MF and PR in the dairy cow appears to include 3 main interrelated mechanisms: 1), decreased synthesis of cyclooxygenase products (mainly PgF2 alpha) via precalving linolenic acid-rich diets; 2), lack of calcium availability at calving via calcium-rich diets before calving; 3), intensive lipomobilization around calving due to a lack of energy supply. The risk for both MF and PR appears to be maximum with old cows fed diets rich in green fodder (fresh or ensiled) and in calcium, and poor in cereals in the prepartum period.

Animal Nutritional Physiological Phenomena↗

PubMed1990

Factors and markers of virulence in Escherichia coli from human septicemia.

One hundred Escherichia coli isolates from human septicemia were characterized with respect to O serogroups 1, 2, 4, 6, 7, 8, 15, 18, 75 and 78, alpha-hemolysin, carboxylesterase B typing, cytotoxic necrotizing factor, F165 and CS31A fimbrial antigens, aerobactin production, colicins, and antibiotic sensitivity. A factorial analysis of correspondence and chi 2 tests indicated that most of E. coli isolates belonging to the studied O serogroups were positive for the virulence factors or markers alpha-haemolysin, carboxylesterase B2 type, cytotoxic necrotizing factor, F165 fimbrial antigen and were antibiotic-sensitive (Group I). These characteristics differentiated them from E. coli isolates from other O serogroups which were generally antibiotic resistant and negative for the cited virulence factors and markers (Group II). Aerobactin and colicin production did not differentiate the two E. coli groups. E. coli O serogroups 4 and 6 were highly represented in group I and were responsible for most of the differences between the two groups.

Anti-Bacterial Agents↗

PubMed1989

[Experimental equine fascioliasis: evolution of serologic, enzymatic and parasitic parameters].

Three, four, and one horses were respectively infected with 100, 1,000, and 5,000 metacercariae of Fasciola hepatica. Six of them were reinfected 38 weeks later with 1,000 metacercariae each. Specific antibodies assayed by counter-electrophoresis, passive hemagglutination and ELISA tests appeared three to six weeks post-infection and peaked 10 to 17 weeks post-infection. Horses infected by 1,000 metacercariae and more showed 17.6% of positive samples by counter-electrophoresis, 49.2% by ELISA, and 75.6% by passive hemagglutination. Plasma glutamate dehydrogenase and gamma-glutamyltransferase levels increased significantly 3 to 5 months post-infection in the most infected animals. Eggs of Fasciola hepatica were only observed in 2 of the 8 horses, 14 and 15 weeks post-infection. This last observation indicates the limits of fecal examination in the diagnosis of fascioliasis in horses.

Animals↗

PubMed1988

[Morbidity registries of animal diseases: development, examples, trends].

The earliest registries of animal morbidity were established at the end of the nineteenth century and concerned contagious diseases. In the 1970s, the development of modern managerial methods on stock farms implied more systematized collection of statistics on clinical disorders. Since breeders are the principal observers of diseases, and because such sources of information as hospitals do not exist for animals, morbidity analysis must be based on surveys especially constructed and relying upon the participation of volunteers. We have cited here the example of ecopathological surveys based on volunteer stock farms and conducted in France at the level of cattle herds. The future of animal pathology registries should be based upon procedures using randomly selected samples, as in the system recently established in France by the Agronomic Research and Statistical Services of the Ministry of Agriculture.

Animal Diseases↗

PubMed1986

[Continuing eco-pathological survey: 4. Demonstration of pathologic associations in dairy cow stock: herd data].

Data concerning frequencies of 17 main diseases in 59 dairy farms from continual eco-pathological survey, are analyzed by classical regression test, principal components analysis and analysis of variance. Thirty-three statistical relations (positive simple correlations) are shown. Clinical mastitis and retained placenta are associated to nine diseases each, metritis, stillbirth and non-infectious foot disorders to six, dystocia and infectious foot disorders to four, pathology of calf and abortion to three, mammary edema and appetite disorders to two. Ovarian pathology, digestive disorders and teat lesions are "isolated", being associated to only one other disease. The pathological frequencies in dairy farms seem to change in the same way, indicating the existence of common risk factors, and leading to propose a global hygienic prevention. The pathological associations allow to definite some groups of dairy farms showing one or other dominant pathological complex. In the frame of our sample of dairy farms, two groups seem to be determined: one with calving disorders and calf pathology centred on retained placenta, the other with foot disorders, infectious and metabolic troubles, centred on mastitis, as dominant diseases.

Abortion, Veterinary↗

PubMed1986

[Continuing eco-pathological survey: 5. Demonstration of pathologic associations in dairy cow stock: individual data].

The calculation of pathological association ratio (or relative risk) from two ways contingency tables, allowed to show numerous interrelationships between diseases of 1,205 dairy cows. The analysis concerns the animal during productive life, the lactation (all lactation mixed), a particular one (1st, 2nd, 3rd and more) and the beginning of productive life of cow (three first lactations). The relationships are discussed according to the level of calculation (animal, lactation, or beginning of life). There is some triangular relations: calving trilogy (dystocia-stillbirth-retained placenta), infectious trilogy (foul of the foot-metritis-mastitis), metabolic trilogy (appetite disorders-ketosis-digestive disorders), foot disorders trilogy (lameness-non infectious diseases of foot-foul of the foot). These pathological groups seem to depend from common risk factors (climate-feeding-cowshed hygiene-control of heat-age-culling policy-genetic factors-anatomical or physiological predisposition, animal size...), but, numerous interactions between groups are also shown, leading to consider pathology in farm like a complex entity.

Age Factors↗

PubMed1986

[Automated microanalysis of plasma ceruloplasmin for the measurement of oxidase activity in cattle and sheep].

An automated method for the determination of plasma ceruloplasmin in bovine and ovine species is described. The analytic principle of Holmberg and Laurell (1951), based on protein oxidase activity, has been applied to a discrete computerized analyzer (Isamat, Isa Biologie, 94230 Cachan, France). Using this method a single operator can determine the oxidase activity of 500 plasma ceruloplasmin samples per day. The method is accurate (2.6% precision for mean values), linear over a range of 7-240 mcg/100 ml in copper equivalents and low in cost because small volumes of reagents can be used; it also correlates well with results from standard atomic absorption spectrophotometry and single radial immunodiffusion methods (correlation coefficients: 0.944 to 0.968). The conservation of oxidase activity is adequate and plasma ceruloplasmin oxidase activity determination can be used for assessing copper nutritional state and for screening inflammatory processes in bovine and ovine herds.

Animals↗

PubMed1986

[Automated micro-determination of bovine plasma 3-hydroxybutyrate without deproteinization].

An automated method for the determination of plasma 3-hydroxybutyrate (3-HB) is described. The enzymatic principle of Williamson and Mellanby is applied to a discrete computerized analyzer (Isamat, Isa-Biologie, 94230 Cachan, France). Using this method, a single operator can determine each day the 3-HB content of 500-600 bovine plasma samples, without perchloric acid precipitation and without adding hydrazine. Aceto-acetate and acetone don't interfere in 3-HB determination. Addition of oxalate (5 mM) in Tris buffer abolishes the interference of lactate dehydrogenase (LDH). The method is accurate, with a precision of 2.8%, linear over the range 0.05-8 mM, low in cost because it needs small volumes of reagent, and correlates well with results from standard end-point manual method (common variance between the two methods: 94-98%). The method allows to use plasma 3-HB determination for assessing nutritional state of cow herds after a convenient standardization of sampling.

3-Hydroxybutyric Acid↗

PubMed1986

[Continuing eco-pathological survey: the objectives and their accomplishment, the choice and the typology of the herd].

The Continuous Eco-Pathological Survey (EEPC) is a prospective and longitudinal epidemiological inquiry, designed to provide data on the global and special pathology in ovine and bovine species, particularly in the dairy cow. The chief purposes of this survey are: to get a better knowledge of the clinical pathology, observed both by the farmer and the veterinarian if he is consulted and the subclinical pathology, detected by various blood tests on 25% of the adult females in each herd; to look on the possible relationships between the herd frequencies of the major disease, i.e. "pathological associations"; to confirm classical or find out new risk factors linked with the environment of the farms; to propose a global approach for the prevention of the main diseases. In this article, methods of choice for herds and animals, and characteristics of the farms studied are described, and the state of realization of the chief objectives of the work is analysed. Conclusion concerns the future of our global approach of the pathology in ruminants.

Animals↗