Search PubMed⌕ Search

Biomedical subjects

J Andrade

Publications and source records attributed to J Andrade.

At least 73 records · Page 4Linked to original sources
PubMed1976

[The planning of higher or university education and the concept of politics, strategy and planning].

The phenomenon of mass education and the crisis of university education in Latin America, in particular in the 1960's, are broadly analyzed. From the facts that characterized the period in question, two conclusions may be drawn: (1) that the basic role of the university must be redefined on the basis of new principles and different content; and (2) that a university must be administered by a system other than improvization. The concepts of policy, strategy, and plan are considered in depth and then explained as they apply to curriculum planning and design.

Education↗
PubMedDate not supplied

Properties of firefly luciferase immobilized through a biotin carboxyl carrier protein domain.

A fusion protein, consisting of biotin carboxyl carrier protein (BCCP) domain from Escherichia coli and firefly luciferase (FL) from Photinus pyralis, was immobilized through the biotin-avidin interaction on 6% cross-linked agarose beads. Several properties of the immobilized BCCP-FL were studied. Immobilized and free enzymes showed no significant difference in thermal stability; both retained at least 91% activity after incubation at 4 degrees C and 25 degrees C for 22 h. Incubation at 37 degrees C for 22 h caused significant activity loss. K(M) and k(cat) values were determined for both free and immobilized enzymes. K(M) values were similar between free and immobilized enzymes; however, k(cat) of immobilized BCCP-FL was one-third of the k(cat) of the free enzyme. 294 micromol/L Co-enzyme A (CoA) and 44 mmol/L dithiothreitol (DTT) enhanced the total bioluminescence output. Triton X-100, Tween 20, PEG 8,000, PVP 40,000 and PVP 360,000 did not enhance the bioluminescence reaction of immobilized BCCP-FL.

Acetyl-CoA Carboxylase↗
PubMedDate not supplied

Contribution of finger tracing to the recognition of Chinese characters.

Finger tracing is a simulation of the act of writing without the use of pen and paper. It is claimed to help in the processing of Chinese characters, possibly by providing additional motor coding. In this study, blindfolded subjects were equally good at identifying Chinese characters and novel visual stimuli through passive movements made with the index finger of the preferred hand and those made with the last finger of that hand. This suggests that finger tracing provides a relatively high level of coding specific to individual characters, but non-specific to motor effectors. Beginning each stroke from the same location, i.e. removing spatial information, impaired recognition of the familiar characters and the novel nonsense figures. Passively tracing the strokes in a random sequence also impaired recognition of the characters. These results therefore suggest that the beneficial effect of finger tracing on writing or recall of Chinese characters is mediated by sequence and spatial information embedded in the motor movements, and that proprioceptive channel may play a part in mediating visuo-spatial information. Finger tracing may be a useful strategy for remediation of Chinese language impairments.

Adult↗
PubMedDate not supplied

Is CK-MB isoenzyme useful for diagnosis of cardiac involvement in icteric leptospirosis?

In the absence of heart failure or cardiogenic shock, cardiac involvement diagnosis in icteric leptospirosis is possible on the basis of abnormal electrocardiograms. As metabolic and electrolytic disorders are frequently seen during acute leptospirosis infection, they may be responsible for some electrocardiograms changes. We conducted a study to assess if creatine phosphokinase isoenzyme determinations are useful in selecting patients with a high cardiac involvement suspicion. Sixty-nine patients were studied prospectively. Ten patients out of 16 with cardiac involvement and 25 without had high CK-MB levels (p > 0.05), although mean values of abnormal CK-MB levels were higher in the group with cardiac involvement (p < 0.05). Our analysis indicates that serum CK-MB determination does not provide a specific indicator of myocardial involvement in the course of icteric leptospirosis.

Adult↗
PubMedDate not supplied

[Leptospirosis in children in Rio do Janeiro].

In order to obtain data about clinical manifestations of symptomatic leptospiral infection in children, the authors reviewed 188 microscopic agglutination tests performed on sera of patients aged 0 to 12 years, made at the National Reference Laboratory of Leptospirosis (FIOCRUZ-RJ) from January 1983 to June 1991. Fifty two (27.6%) sera were positive. Twenty three (12.2%) children had serological evidence of acute infection. The most frequent signs and symptoms of these 23 cases were: fever (100%); myalgia (69.5%); headache (52.1%); jaundice (47.8%); vomit (34.8%); abdominal pain, hemorrhagic manifestations and impaired renal function (17.4%); conjunctivitis (13%); hepatomegaly (4.3%).

Age Distribution↗
PubMedDate not supplied

Leptospirosis patient with AIDS: the first case reported.

A case of renal icterohemorrhagic leptospirosis involving a patient with acquired immunodeficiency syndrome (AIDS) is reported. Despite the low levels of CD4+ T lymphocytes, the clinical course of leptospirosis was similar to that observed in non-immunodepressed patients, and no worsening of AIDS occurred due to the infection by the spirochete. Serologic conversion was observed in the microscopic agglutination test, with maximum titer of 1:3,200. The patient had positive urine cultures for Leptospira interrogans for two months, whereas blood cultures were negative.

AIDS-Related Opportunistic Infections↗
PubMedDate not supplied

[An anti-Listeria seroepidemiological study in Uberaba, MG].

From a total of 445 individuals, 17.1% had antibodies against L. monocytogenes detected by the agglutination tube test. They were separated in seven groups: bloods donnors (n = 50), Hospital visitors (n = 40), frigorific workers (n = 28), aviculture workers (n = 87), herdsman (n = 31), agriculture students (n = 60) and street-sweepers (n = 51). L1/2a serotype was predominant. Individuals from urban areas (19.5%) and those who had less contact with animals (21.7%) had significantly positive serology when compared with individuals from rural areas (9.4%) and those who had close contact with animals (13.2%). The overall picture is individuals of more specialized occupations had more frequently (25.9%) anti listeria antibodies similar to the results observed in developed countries where listeriosis is a public health problem in urban areas.

Adolescent↗
PubMedDate not supplied

[Epidemiology of human leptospirosis, with special reference to greater Rio de Janeiro, Brazil, from 1970 to 1982].

From 1970 to 1982, 884 cases of human leptospirosis were serologically diagnosed at the Leptospirosis Laboratory, Fundação Oswaldo Cruz - FIOCRUZ, by means of the microscopic agglutination test. Of these patients, 775 were hospitalized in the city of Rio de Janeiro, and 109 were reported by other Brazilian cities. Icterohaemorrhagiae was the presumptive infecting serogroup most commonly encountered in greater Rio patients (76.4%) and in the others (41.3%). During this 13-year period, two Icterohaemorrhagiae strains were isolated. In greater Rio the illness predominated between January and April (53.7%), the male sex accounted for 89.9% of the cases, and patients ranging from 16 to 45 year-olds were the most affected (74.9%).

Adolescent↗