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Biomedical subjects

J Abraham

Publications and source records attributed to J Abraham.

At least 145 records · Page 8Linked to original sources
PubMed1975

Regional cerebral blood flow changes after bilateral external carotid artery ligation in acute experimental infarction.

Regional cerebral blood flow (rCBF) was measured in baboons by intracarotid injection of 133Xe and a gamma camera after acute cerebral infarction was induced by occlusion of the middle cerebral artery (MCA). A steady state of rCBF was measured four hour after MCA occlusion and was followed by bilateral ligation of the external cartoid arteries (ECA). Subsequent rCBF measurements were obtained at 30, 60, and 120 minutes. After bilateral ECA ligation, flow in ischaemic and non-ischaemic areas was greatly enhanced and flow in the hyperaemic areas significantly reduced, presumably since they had provided collateral circulation to the ischaemic zone with a favourable redistribution.

Acute Disease↗
PubMed1975

Improved rapid method for electron microscopy of macro- and micro-tissue cultures.

A rapid method of embedding macro- and micro-tissue cultures is described. The procedure involves use of a preshaped BEEM capsule for pelleting, fixation and embedding of tissue culture cells for electron microscopy. To obtain good contrast specimens prepared by this rapid method need a rather longer staining time with uranyl acetate than specimens prepared by standard methods. THis procedure takes only about 200 min from fixation and embedding to double staining with uranyl acetate and lead citrate. It should be useful for both monolayer tissue cultures and organ cultures in diagnostic and research studies on viral agents.

Culture Techniques↗
PubMed1975

The influence of changes in arterial CO2 and blood pressure on the collateral circulation and the regional perfusion pressure in monkeys with occlusion of the middle cerebral artery.

In 7 baboons and 5 macaques the effects of hypercapnia, hypocapnia, hypertension, and combinations of hypertension plus hypocapnia and of hypertension plus hypercapnia on the regional perfusion pressure (rPP) in the brain area rendered ischaemic by occlusion of the middle cerebral artery (MCA) were studied. A new technique for recording the pressure in the occluded MCA was used. The regional tissue pressure (rTP) was recorded with the so called wick type pressure transducers. Hypercapnia produced a marked decrease in rPP in the ischaemic brain area. Hypertension, especially in combination with hypocapnia, produced a very pronounced increase in rPP in the ischaemic brain area. The clinical implications of these findings are discussed. Occlusion of the homolateral common carotid artery (CCA) was followed by a very slight and transient drop in the occluded MCA pressure. Occlusion of the contralateral CCA was followed by a marked and persistent drop in the occluded MCA pressure.

Animals↗