Biomedical subjects

Issarang Nuchprayoon

Publications and source records attributed to Issarang Nuchprayoon.

22 records · Page 2Linked to original sources

PubMed2003

Comparative study on serum iron determination by different methods.

Iron is an important trace element in humans. Depletion or overload iron status can result in a number of aberrant physiological functions. Determination of serum iron is one of the valuable test panels for iron status assessment. A comparative study was performed on 3 different methods of serum iron determination, nonprecipitating ferrozine colorimetric method, precipitating ferrozine colorimetric method, and iron liquicolor cleaning factor colorimetric agar-based (CAB) method (Human, Taunusstein, Germany). The correlation coefficients between nonprecipitating ferrozine colorimetric method and precipitating ferrozine colorimetric method, between nonprecipitating ferrozine colorimetric method and iron liquicolor cleaning factor CAB method, and between precipitating ferrozine colorimetric method and iron liquicolor cleaning factor CAB method were found to be 0.998 (P = .067), 0.999 (P = .067), and 0.951 (P = .934), respectively. For a setting with limited resources such as Thailand, use of the first analytical technique, nonprecipitating ferrozine colorimetric method, is recommended because of its low cost and ease of performance.

Colorimetry↗

PubMed2003

Prevalence of bancroftian filariasis on the Thai-Myanmar border.

To achieve the goal of eliminating lymphatic filariasis by the year 2020, close monitoring systems and effective control strategies need to be implemented and the real disease burden needs to be assessed. Bancroftian filariasis is endemic at the Thai-Myanmar border. However, there are only limited data on the prevalence of this disease in Thailand available. We employed microscopic examination, together with ELISA kits to detect W. bancrofti-specific Og4C3 circulating antigen and specific anti-filarial IgG4 antibodies to determine the burden of bancroftian filariasis in an endemic area at the Thai-Myanmar border in Umphang District, Tak province, Thailand. A total of 433 Thai-Karen blood samples were analyzed. The microfilarial rate determined by microscope was 6% and the W. bancrofti-specific Og4C3 antigenemia rate was 22%, while the specific anti-filarial IgG4 antibody rate was 54%. There were statistically significant higher levels of W. bancrofti-specific Og4C3 antigen in the microfilaremic-antigenemic group than in the amicrofilaremic-antigenemic group (unpaired Student's t-test; p < 0.001), similar to the specific anti-filarial IgG4 antibody results (unpaired Student's t-test; p < 0.001). A statistically significant correlation of moderate degree between the presence of W. bancrofti-specific Og4C3 antigen and of specific anti-filarial IgG4 antibody was found in the amicrofilaremic group (r = 0.474, p < 0.001), but not in the microfilaremic group (r = 0.291, p > 0.05). Our study revealed a very high prevalence of bancroftian filariasis in this endemic area and thus emphasized the importance of using highly sensitive and specific diagnostic tools to evaluate the true prevalence of the disease.

Adolescent↗

PubMed2002

Lyophilized cryoprecipitate for children with hemophilia A.

Cryoprecipitate is the most cost-effective treatment for bleeding events in hemophilia A children in Thailand. Lyophilized cryoprecipitate (LC), a freeze-dried heat-treated blood product from the National Blood Center, can be stored in a refrigerator. The objectives of this study were to assess the efficacy of LC in bleeding episodes of children with hemophilia A and to determine the pharmacokinetics of single dose LC infusion. Eight male children with congenital factor VIII deficiency, aged 1.2-13 years, with 12 active bleeding episodes were infused with reconsitituted LC (average factor VIII activity = 236 IU per bottle) at the dose of 1 bottle per 11 kg. All patients achieved clinical hemostasis by 12 hours post-infusion. The Factor VIII activity reached peak level within the first hour and declined slowly in the subsequent hours and retained in vivo Factor VIII activity at 27.3 +/- 5.6 per cent at 12 hours post-infusion. The incremental factor VIII activity was 2.37 +/- 1.05 per cent (mean +/- SD) per U infused per kilogram body weight. The half-life of infused factor VIII was 10.5 hours. The authors concluded that reconstituted LC infusion is efficacious in the treatment of bleeding episodes in children with hemophilia. Cost-effectiveness of this product and cryoprecipitate and other purified factor VIII should be evaluated.

Adolescent↗

PubMed2002

Prevalence of parasitic infections among Thai patients at the King Chulalongkorn Memorial Hospital, Bangkok, Thailand.

Parasitic diseases are still considered to be a major public health problem. Most patients with parasitic infections are asymptomatic and therefore remain undetected. Asymptomatic parasitic infections are usually discovered by routine parasite examination. To determine the result of parasite examination at the Parasitology Unit, Out Patient Department, King Chulalongkorn Memorial Hospital, Bangkok, Thailand, the authors collected the data of individuals examined for parasite infections from June to December 1997. A total of 6,231 Thais provided the data for analysis. Evidence of parasitic infections was found in 557 (8.94%) cases. The disease was most prevalent in males (57.3%), and in the age group >15-30 years old (11.13%). The population from the Northeast of Thailand was found to harbor parasites with the highest prevalence rate (17.03%), while it was 11.90 per cent in the northern group. The parasitic prevalence rates in the West, East, South and Central regions were 10.60 per cent, 8.90 per cent, 7.74 per cent, and 4.92 per cent, respectively. The parasite most commonly identified was Strongyloides stercoralis (33.39%), while giardiasis was the most common protozoan infection (14.36%). The highest infection rates of S. stercoralis, hookworms, Opisthorchis viverrini, and Gnathostoma spinigerum were found in northeasterners. People from the North of Thailand were infected mostly with G. lamblia. People of working-age from northeastern as well as northern regions harbored pathogenic parasites with high prevalence rates. To prevent parasitic infections, health education for these high risk groups should be provided.

Adolescent↗