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Biomedical subjects

I Ueno

Publications and source records attributed to I Ueno.

At least 73 records · Page 4Linked to original sources
PubMed1984

Interaction between quercetin and superoxide radicals. Reduction of the quercetin mutagenicity.

Interaction between quercetin and superoxide radicals was investigated using xanthine-xanthine oxidase system and potassium superoxide as superoxide radical generators. Superoxide radical scavenging action of quercetin was demonstrated by measurement of the electron spin resonance spectrum of the 5-hydroperoxy-2,2-dimethyl-1-pyrrolidinyloxyl adduct. Degradation of quercetin by the radicals was demonstrated spectrophotometrically. Reduction of the quercetin mutagenicity by the radicals was demonstrated in the Salmonella typhimurium strain TA 98 by the Ames test.

Animals↗
PubMed1984

[The relationship between blood alcohol level and head injury].

Alcoholic intoxication and a traumatic intracranial lesion have many features in common. It is important for a doctor not to make an incorrect diagnosis between these two conditions, but it is difficult in some cases. The effect of alcohol on the conscious level may complicate the assessment of patients and make it more difficult to recognize the development of complications such as intracranial hematoma. The most immediate and correct diagnosis can be obtained by estimating the amount of alcohol in a patient's blood although it is often difficult in the usual emergency departments in Japan. To try to throw some light on this question, we decided to attempt both clinical and laboratory diagnosis of alcohol ingestion. In 68 adult patients who had head injuries with conscious disturbance admitted to our ward at night, alcohol levels in their blood were estimated by gas chromatography, during the past 24 months. At the same time clinical examinations for the diagnosis of drunkenness were made: on admission, it was recorded whether all patients had white or red conjunctiva, whether speech was clear or slurred, whether there was a distinctive smell on their breath, and whether coordination was good or poor. Of 68 patients (63 male, 5 female), 7 had no alcohol in their blood and 61 had positive alcohol results. The mean blood alcohol level was 233 +/- 130 mg/dl. Clinical diagnosis of alcohol ingestion; The amount of alcohol in the blood correlated highly with the strength of the smell on their breath and of conjunctival injection (p less than 0.001), on the other hand, did not correlate with the speech disturbance, skillfullness of coordination and level of consciousness.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1983

Carcinogenic activity of Farfugium japonicum and Senecio cannabifolius.

The carcinogenicity of Farfugium japonicum and Senecio cannabifolius was studied in ACI rats. In Group I, rats were given a diet containing 20% Farfugium japonicum. Groups II, III, IV and V were given diets containing 8%, 4%, 1% and 0.2% Senecio cannabifolius until the end of the experiment, respectively. The experiment was terminated after 480 days, except for Group V which was terminated 560 days after the start of feeding. Hemangioendothelial sarcoma of the liver and liver cell adenoma were induced in Groups I, IV and V. All rats in Groups II and III died of hepatotoxicity within a short period.

Adenoma↗
PubMed1983

Enhancing effect of dextran sulfate sodium on colorectal carcinogenesis by 1,2-dimethylhydrazine in rats.

Four groups were used, each consisting of 24 male ACI rats. Group I received a single subcutaneous injection of 20 mg 1,2-dimethylhydrazine (DMH)/kg body weight and was fed on basal diet until the termination of the experiment. Group II received the same single injection as in group I and then, starting one week later, was fed the diet containing 1% dextran sulfate sodium (DS-M-1). Group III received an injection of 0.9% sodium chloride and then DS-M-1 diet. The control group was given neither DMH nor DS-M-1 diet. The experiment was terminated after 40 weeks. A significantly higher incidence of colorectal carcinoma was observed in group II, as compared with group I (P less than 0.02). These data demonstrate the enhancing effect of DS-M-1 on colorectal carcinogenesis.

1,2-Dimethylhydrazine↗
PubMed1983

Metabolic fate of [14C] quercetin in the ACI rat.

To explain why quercetin, a mutagenic flavonoid distributed widely in edible plants, showed no carcinogenicity in experimental animals, overall metabolic fate of the drug was pursued using radiolabeled compound. [4-(14)C]Quercetin was administered orally or intraperitoneally to male ACI rats and its distribution, metabolism and excretion were studied by autoradiography and/or quantitation of radioactivity, and chemical analysis. [14C]Quercetin was also administered intravenously, intraperitoneally or orally to bile duct-cannulated rats and radioactivities in CO2 expired, bile, urine and feces were measured. To elucidate chemical structure of radiolabeled flavonoids in the bile and urine, urinary flavonoids derived from the rats given non-labeled quercetin diet was analyzed chemically. When [14C]-quercetin was administered orally, around 20% of the administered [14C]quercetin was absorbed from the digestive tract, more than 30% was decomposed to yield 14CO2 and around 30% was excreted unchanged in the feces. The absorbed [14C]quercetin was rapidly excreted into the bile and urine within 48 hours as the glucuronide and sulfate conjugates of [14C]quercetin, 3'-O-monomethyl [14C]quercetin and 4'-O-monomethyl [14C]quercetin. The present data indicate that the lack of carcinogenicity of quercetin in the rat is attributable to neither its poor absorption from the digestive tract nor its microbial degradation in the tract. Efficient metabolism and elimination of quercetin may be one reason of the lack of carcinogenicity.

Animals↗
PubMed1982

Effect of retinoic acid and 12-O-tetradecanoyl phorbol-13-acetate on the binding of epidermal growth factor to its cellular receptors.

Binding of 125I-labelled epidermal growth factor (EGF) to C3H/2K cells and the effect of a tumor promotor, 12-O-tetradecanoyl phorbol-13-acetate (TPA) and of a tumor promotor antagonist, retinoic acid, on the binding was studied. Scatchard plot analysis of the binding showed the presence of two types of binding sites with different affinity to EGF. Treatment of the cells with retinoic acid for 1 h resulted in elevation of the affinity of both sites without changing their number per cell. Prolonged exposure to retinoic acid abrogated this elevation of the affinity and caused cycloheximide-sensitive increase of the number of the binding sites of both types. TPA inhibited binding of EGF to the cells by abolishing the binding to the high affinity sites, whereas retinoic acid, in the presence of TPA, enhanced it by increasing the number of the low affinity sites.

Animals↗
PubMed1981

Carcinogenicity examination of quercetin and rutin in ACI rats.

Carcinogenicity of quercetin and rutin were examined in inbred ACI strain rats. Rats were given a diet containing 1% or 5% quercetin or 5% rutin for 540 days, or 10% quercetin and 10% rutin for 850 days. Rats in control groups were fed a normal basal diet. Most tumors found in experimental groups were also found in the corresponding control groups. Furthermore, there was no significant difference between the incidence of tumors in the experimental or control groups (P greater than 0.05). Thus, quercetin and rutin tested were not shown to be carcinogenic to ACI rats.

Animals↗
PubMed1981

Tumor induction in germ-free rats fed bracken (Pteridium aquilinum).

This study indicated that there was no significant difference in the incidence of intestinal tumors between germ-free and conventional rats fed a diet containing bracken, suggesting that gut microflora did not play a definite role in bracken tumorigenesis. However, bracken induced exclusively sarcoma but no adenocarcinoma in germ-free rats, whereas it induced predominantly adenocarcinoma in conventional rats.

Animals↗
PubMed1980

Chronic toxicity and hepatocarcinogenicity of (+) rugulosin, an anthraquinoid mycotoxin from penicillium species: preliminary surveys in mice.

(+) Rugulosin (RUG) is bis-anthraquinoid pigment produced by fungi such as Penicillium rugulosum Thom, P. tardum and others, and its chemical structure is very close to (-) luteoskyrin, one of bis-anthraquinoid pigments of Penicillium islandicum Sopp and hepatocarcinogenic to mice and rats. This investigation was concerned with the subacute and chronic effects of RUG on hepatic injury and hepatocellular tumor induction in male mice fed purified diets for 22 days containing 1.5 mg/5 g diet/animal per day or whole feeding period containing 0.3 and 0.75 mg RUG/5 g diet/animal per day. A half of male DDD mice receiving 1.5 mg RUG for the initial 22 days of feeding died 1-3 weeks thereafter from the acute hepatic injury characterized by fatty degeneration and liver cell necrosis, and hepatocellular carcinoma developed in one out of 14 mice surviving the feeding of control diet for additional 400 days. Each one fourth of cases of male ddYS mice receiving 0.3 and 0.75 mg RUG for 500-700 days developed hyperplastic nodules of liver cells. These data suggest that RUG is possibly a weak hepatocarcinogen for male mice with a potency les than one tenth of (-) luteoskyrin.

Animals↗
PubMed1979

Inhibitory effects of condiments and herbal drugs on the growth and toxin production of toxigenic fungi.

The effects of thirteen kinds of powdered herbal drugs and seven kinds of commercial dry condiments on the growth and toxin production of Aspergillus parasiticus, A. flavus, A. ochraceus, and A. versicolor were observed by introducing these substances into culture media for mycotoxin production. Of the twenty samples tested, cinnamon bark completely inhibited the fungal growth, while the others only inhibited the toxin production. The inhibitors were easily extracted from the samples with solvents such as hot water, chloroform, or ethanol. The extracts from coptis, philodendron bark, mustard, green tea leaves, and zanthoxylum completely inhibited the aflatoxin production of A. parasiticus, however, they had little or no inhibitory effect against A. flavus.

Aspergillus↗
PubMed1979

Induction of hepatic tumors in rats by senkirkine and symphytine.

The carcinogenicity of the pyrrolizidine alkaloids senkirkine and symphytine was studied in male inbred ACI rats. Animals were divided into 3 groups: Group I received ip injections of freshly prepared senkirkine at a dose of 10% of the median lethal dose (LD50) twice weekly for 4 weeks and then once a week for 52 weeks. Group II received ip injections of symphytine at a dose of 10% of the LD50 by the same injection schedule as in group I. The control group was given ip injections of a 0.9% NaCl solution following the same injection schedule as in experimental groups. All group I rats survived for more than 290 days after the start of injections, and 9 of 20 rats developed liver cell adenoma. All group II animals survived for more than 330 days after the start of injections. Of 20 rats, 4 had liver tumors, 3 had hemangioendothelial sarcomas, and 1 had liver cell adenoma. The hemangioendothelial sarcomas showed metastasis in the lungs of 2 rats. The control group had no liver tumors.

Animals↗