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Biomedical subjects

I Suzuki

Publications and source records attributed to I Suzuki.

At least 199 records · Page 11Linked to original sources
PubMed1991

Sequence-specific interactions of a maize factor with a GC-rich repeat in the phosphoenolpyruvate carboxylase gene.

A plant nuclear protein PEP-I, which binds specifically to the promoter region of the phosphoenolpyruvate carboxylase (PEPC) gene, was identified. Methylation interference analysis and DNA binding assays using synthetic oligonucleotides revealed that PEP-I binds to GC-rich elements. These elements are directly repeated sequences in the promoter region of the PEPC gene and we have suggested that they may be cis-regulatory elements of this gene. The consensus sequence of the element is CCCTCTCCACATCC and the CTCC is essential for binding of PEP-I. PEP-I is present in the nuclear extracts of green leaves, where the PEPC gene is expressed. However, no binding was detected in tissues where the PEPC gene is not expressed in vivo, such as roots or etiolated leaves. Thus, PEP-I is the first factor identified in plants which has different binding activity in light-grown compared with dark-grown tissue. PEP-I binding is also tissue-specific, suggesting that PEP-I may function to coordinate PEPC gene expression with respect to light and tissue specificity. This report describes the identification and characterization of the sequences required for PEP-I binding.

Base Composition↗
PubMed1991

Immunocytochemical localization of laminin in the epithelial rests of Malassez of immature rat molars.

Immediately after disruption of Hertwig's root sheath, epithelial cells were found near the root apex, singly or in groups of two or three cells. They were irregular in shape with only a partial lining of basal lamina. Immunoreactivity for laminin was intense in the basal lamina and weak between projections of the epithelial cells. In 5-week-old rats, the epithelial rests consisted of about five cells and still had an incomplete basal lamina with collagen fibrils in the intercellular spaces. Immunoreaction products were seen in the basal lamina and diffusely in the intercellular spaces. The epithelial rests of 9-week-old rats had more cells, an almost complete lining of basal lamina, and narrowed intercellular spaces. Immunoreaction products were seen in the basal lamina but not in the intercellular spaces. These findings indicate the basal lamina is involved in the formation of the epithelial rests.

Animals↗
PubMed1991

Oral administration of SSG, a beta-glucan obtained from Sclerotinia sclerotiorum, affects the function of Peyer's patch cells.

The effect of orally administered SSG, a beta-1,3-glucan obtained from the culture filtrate of a fungus, Sclerotinia sclerotiorum IFO 9395, on the function of Peyer's patch (PP) cells was investigated in comparison with that on spleen cells in mice. Oral administration of SSG enhanced the proliferative response of PP cells to a T-cell mitogen, concanavalin A (Con A), and a B-cell mitogen, lipopolysaccharide (LPS), although the response of spleen cells was not affected. Peyer's patch cells taken from mice which had received oral administration of SSG two days before, showed enhanced plaque-forming cell (PFC) response to sheep red blood cells (SRBC) after antigen (SRBC) stimulation for 5 days in vitro. These results suggest that oral administration of SSG can modulate the mucosal immune response.

Adjuvants, Immunologic↗
PubMed1991

Chai-ling-tang (Japanese name: sairei-to) as an oral adjuvant.

Immunomodulating and anti-tumor activities of orally administered Chai-Ling-Tang (Japanese name: sairei-to, ST) were investigated. The oral administration of ST into mice augmented the antibody response to intraperitoneally administered 2, 4, 6-trinitrophenyl-haptenated sheep red blood cells (TNP-SRBC). Orally administered ST showed also an enhancing effect on the antibody response to TNP-SRBC administered by the oral route. In addition, orally administered ST markedly activated the peritoneal macrophages to enhanced phagocytic and lysosomal enzyme activities. A significant inhibition of tumor growth was observed in a syngeneic tumor-mouse system when ST was administered orally. These results suggest that ST has an efficiency as an oral adjuvant or an oral biological response modifier (BRM).

Adjuvants, Immunologic↗
PubMed1991

Oral administration of a streptococcal agent OK-432 activates peritoneal macrophages in mice.

The effect of orally administered OK-432, a streptococcal preparation, on the function of peritoneal macrophages in mice was examined. The administration of OK-432 orally (1 KE or 2 KE, four times every three days) did not affect the numbers of both total peritoneal cells and macrophages recovered five days after the final administration. However, the macrophages exhibited an increase in their spreading ability. Other functions of the peritoneal macrophages including lysosomal enzyme activity, phagocytic activity and interleukin 1 (IL-1) production were also enhanced significantly by the oral administration of OK-432 (1 KE or 2 KE). The production of H2O2, however, was not affected by the same treatment with OK-432. The activation of peritoneal macrophages by orally administered OK-432 reported here may contribute to expansion of the clinical application of this drug.

Administration, Oral↗
PubMed1991

Atypical multiple benign osteoblastomas accompanied by simple bone cysts. A case report.

A case of atypical benign osteoblastomas occurring at multiple sites in the maxilla and mandible of a 38-year-old woman, in association with simple bone cysts, is described. The tumors produced hazy or ground-glass-like semiradiolucent lesions with well-defined or poorly-defined margins on conventional radiographs. Histologically, the lesions were characterized by a proliferation of plump osteoblast-like cells forming trabeculae of osteoid and immature bone in a well-vascularized stroma. In addition, simple bone cysts associated with a radiolucent-radiopaque lesion consisting of lamellar bone and loose connective tissue were found next to one of the tumours. Possible pathogenesis of the tumours and cysts is discussed.

Adult↗
PubMed1991

Interaction of doxapram and pentobarbital in mice.

We found a statistically significant increase in duration of pentobarbital-induced narcosis in doxapram-treated mice. The influence of doxapram (a respiratory stimulant) pretreatment on pentobarbital metabolism in mice was assessed by measurements of sleeping times, hypothermia, LD50 values, hepatic microsomal metabolism and relative plasma and brain levels of pentobarbital. When doxapram was given intraperitoneally 60 min. prior to administration of pentobarbital, doxapram potentiated pentobarbital-induced narcosis in a dose- and time-dependent manner, but had no effect on onset time. Doxapram potentiated hypothermia, increased acute toxicity, and prolonged the pentobarbital half-life in brain and plasma, but measurement of the concentration of pentobarbital in the brain and plasma immediately upon recovery from narcosis showed that there were no differences in any of the groups examined. Also, brain-to-plasma ratios of pentobarbital did not differ between the control and doxapram-treated groups. Doxapram competitively inhibited the hepatic metabolism of pentobarbital in 9000 x g supernatant incubation mixtures. The results obtained from these experiments indicate that inhibition of drug-metabolizing enzymes by doxapram may account for its enhancement of the duration of pentobarbital-induced narcosis.

Animals↗
PubMed1991

Inhibition of experimental pulmonary metastasis of Lewis lung carcinoma by orally administered beta-glucan in mice.

The inhibitory effect on experimental pulmonary metastasis of Lewis lung carcinoma (3LL) of SSG, a (1----3)-beta-D-glucan obtained from the fungus Sclerotinia sclerotiorum IFO 9395, administered orally was examined in mice. Oral administration of SSG for 10 consecutive days just after the intravenous implantation of tumor cells significantly inhibited the experimental pulmonary metastasis of 3LL at a dose of 2000 micrograms. However, SSG administered orally involving other timings was less effective. In comparison with oral administration, SSG was effective when administered intraperitoneally for 10 consecutive days at a dose of 200 micrograms. These results suggest that SSG given by both parenteral and nonparenteral routes is effective in the inhibition of experimental pulmonary metastasis of tumors.

Animals↗
PubMed1991

Effect of intraperitoneally administered beta-1,3-glucan, SSG, obtained from Sclerotinia sclerotiorum IFO 9395 on the functions of murine alveolar macrophages.

The effect of intraperitoneally (i.p.) administered SSG, a beta-1,3-glucan obtained from the culture filtrate of the fungus Sclerotinia sclerotiorum IFO 9395, on the functions of alveolar macrophages (AM) in CDF1 mice was examined. SSG administered i.p. increased the number of AM and enhanced several functions of murine AM (lysosomal enzyme activity, phagocytic activity, candidacidal activity, H2O2 production and interleukin 1 (IL-1) production) at a dose of 250 micrograms/mouse on days 1 and 8, especially on day 8. Furthermore, SSG (250 micrograms/mouse) administered i.p. for 10 consecutive days significantly inhibited the experimental pulmonary metastasis of Lewis Lung Carcinoma (3LL) on BDF1 mice.

Animals↗
PubMed1991

[A case report of atypical coarctation of the aorta with coarctation and left subclavian arterial aplasia].

A 18 year-old female was admitted to our hospital with hypertension of 190/100 mmHg in her right arm and 140/110 mmHg in the left arm. Femoral arterial pulsation was not palpable. Her physical status and growth of the lower limbs were normal. Aortography demonstrated slight coarctation with a pressure gradient of 10 mmHg and descending aortic stenosis with a pressure gradient of 80 mmHg. Main three branches from aortic arch were anatomically normal. However, the left subclavian artery distal to the origin of the left vertebral artery was not visualized and the axillary artery was perfused by collaterals. Atypical coarctation was replaced using a woven Dacron graft of 14 x 40 mm. Postoperatively proximal pressure decreased from 180/113 to 156/98 mmHg and there was no pressure gradient between proximal and distal of the graft. After six months blood pressure in the right arm gradually decreased to 120/78 mmHg.

Adolescent↗
PubMed1990

Input-output organization of reticulospinal neurones, with special reference to connexions with dorsal neck motoneurones in the cat.

Dorsal neck motoneurones receive disynaptic tectal and pyramidal EPSPs via common reticulospinal neurones (RSNs). This study was aimed at identification of the RSNs projecting directly to neck motoneurones and mediating these EPSPs. 1. Stimulation of the tectum and the cerebral peduncle evoked monosynaptic descending volleys in the spinal cord, which were chiefly mediated by reticulospinal neurones in the pons and the medulla. Systematic tracking of the C3 and C7 segments was made to locate descending volleys in the spinal funiculi. The tectal monosynaptic volley was largest in the medial part of the ventral funiculus and decreased gradually as the recording electrode was moved to the lateral part of the ventral funiculus and the lateral funiculus. In contrast, the peduncle-evoked monosynaptic volley was distributed rather evenly in the ventral funiculus and the ventral half of the lateral funiculus. 2. Differences in funicular distribution of the two descending volleys suggest the existence of subgroups of RSNs which differed in strength of inputs from the two descending fibre systems and in the funicular location of descending axons. 3. The RSNs were classified into the following four groups; (1) mRSNs which descended in the medial part of the ventral funiculus, (2) in RSNs which descended in the ventrolateral funiculus, (3) 1RSNs which descended in the dorsal 2/3 of the lateral funiculus and (4) coRSNs which descended in the contralateral funiculi. The mRSNs were located in a fairly localized region corresponding to the nucleus reticularis pontis caudalis (N.r.p.c), while inRSNs, 1RSNs and coRSNs were mainly in the nucleus reticularis gigantocellularis (N.r.g.), in the nucleus reticularis magnocellularis (N.r.m.) and in the nucleus reticularis ventralis (N.r.v.). RSNs were further divided into three types depending on the levels of projection. L-RSNs projected to the lumbar spinal segments. C-RSNs descended to the C6-C7 spinal segment but not to the lumbar segments. N-RSNs projected to the C3 but not to the C6-C7 segments. 4. Stimulation of the tectum and the cerebral peduncle produced monosynaptic negative field potentials in the medial two thirds of the reticular formation in the pons and medulla.(ABSTRACT TRUNCATED AT 400 WORDS)

Action Potentials↗
PubMed1990

Modifications of temporal lobectomy according to the extent of epileptic foci and speech-related areas.

The authors applied combined depth and subdural electrodes in patients with intractable complex partial seizures to detect the precise extent of epileptic foci and functionally map speech-related areas. The medial temporal structures were explored with depth electrodes and the lateral temporal cortex with subdural electrodes. On the speech-dominant side, electrical stimulation was given to demarcate the speech-related areas in the lateral temporal cortex. Based on these data, the extent of surgical resections was tailored to include as much of the epileptogenic areas as possible while preserving the functionally essential zones of the lateral cortex. According to the range of resection, three different approaches were employed for en bloc ablation of the lateral cortex and opening of the inferior ventricle. The results thus acquired have been satisfactory in terms of seizure control and the preservation of speech function.

Adolescent↗
PubMed1990

Effect of orally administered beta-glucan on macrophage function in mice.

The effect of orally administered SSG, a beta-1,3-glucan obtained from the culture filtrate of the fungus Sclerotinia sclerotiorum IFO 9395, on the function of peritoneal macrophages in CDF1 mice was examined. Oral administration of SSG (20, 40, 80 or 160 mg/kg, daily for 10 consecutive days) enhanced the acid phosphatase activity of peritoneal macrophages. The greatest enhancing effect was observed at 80 mg/kg of SSG. Relatively long periods of administration (more than 10 consecutive days) were needed to induce significant enhancing effects. Phagocytic activity, candidacidal activity, hydrogen peroxide (H2O2) production and interleukin-1 (IL-1) production of peritoneal macrophages were also enhanced after the administration of SSG by the oral route (80 or 160 mg/kg). However, the durations of the activated state after completion of administration differed depending on the activity. Enhanced activity of lysosomal enzyme (acid phosphatase) was also shown in peritoneal macrophages taken from C3H/HeJ mice, which is a nonresponder strain to bacterial lipopolysaccharide (LPS). These results demonstrate that SSG given by the oral route can activate peritoneal macrophages in mice.

Adjuvants, Immunologic↗