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Biomedical subjects

I Ohmori

Publications and source records attributed to I Ohmori.

At least 19 recordsLinked to original sources
PubMed1995

The shortest isoform of human vascular endothelial growth factor/vascular permeability factor (VEGF/VPF121) produced by Saccharomyces cerevisiae promotes both angiogenesis and vascular permeability.

Vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) is a multifunctional cytokine that is expressed as four isoforms having 206, 189, 165, and 121 amino acids in humans. We constructed a system that produces the shortest isoform of human VEGF/VPF in Saccharomyces cerevisiae (yVEGF/VPF121). Active yVEGF/VPF121 was secreted from the yeast cells as a glycosylated dimeric protein. Various biological activities of the purified yVEGF/VPF121 were examined. It bound to cell surface receptor(s) and stimulated the growth of human umbilical vein endothelial cells in culture at a dose similar to that of native VEGF/VPF. Purified yVEGF/VPF121 also induced angiogenesis in mice, and promoted the extravasation of plasma proteins from the blood vessels. These observations demonstrated that the shortest isoform of VEGF/VPF with an amino acid sequence of 121 residues contains enough information necessary to trigger both angiogenesis and the induction of vascular permeability upon binding to its cognate receptor(s).

Animals↗
PubMed1995

[A case of localized fibrous tumor of the pleura with bloody pleural effusion].

A 25-year-old female came to our hospital with the chief complaint of right dorsal pain. On her initial chest X-ray, the tumor (5 x 3 cm) was recognized in the right lower lung field. The tumor grew rapidly during the three month period between the initial X-ray taken on her first visit and her getting admitted in the hospital. A preoperative diagnosis was done by percutaneous aspiration biopsy. Initial diagnosis of spindle cell tumor was made. Partial resection of right lower lobe with chest wall resection was carried out. There were about 500 ml of bloody pleural effusion. Macroscopically, the tumor was encapsulated, measuring 8.0 x 6.0 x 5.0 cm. The cross section was of a yellowish brown solid tumor. The histological diagnosis was of a low grade malignant fibrous mesothelioma. Only 14 cases of localized mesothelioma with pleural effusion including our case have been reported in Japan. She is still alive nine months after surgery. This patient should be carefully followed for the recurrence of the disease.

Adult↗
PubMed1994

Vascular endothelial growth factor/vascular permeability factor is detectable in the sera of tumor-bearing mice and cancer patients.

We developed an enzyme-linked immunosorbent assay (ELISA) for vascular endothelial growth factor/vascular permeability factor (VEGF/VPF). The assay revealed that VEGF/VPF levels in the sera of mice were significantly increased from undetectable level by s.c. transplantation with a solid tumor. We also measured VEGF/VPF levels in serum specimens obtained from cancer patients with several types of cancers. VEGF/VPF levels in the sera from cancer patients were significantly higher than those in the sera from the individuals with no sign of cancer.

Animals↗
PubMed1989

[A histological observation of the primary teeth with enamel hypoplasia resulting from perinatal disturbance].

Two primary teeth with enamel hypoplasia from two cerebralpalsied children were subjected to histological examination. One primary tooth was B and the other was C. The location of enamel hypoplasia in these teeth was considered to coincide with the neonatal line reaching the enamel surface. The purpose of this study was to examine the location of the enamel hypoplasia in these teeth in relation to the neonatal line, and to detail the histological structure of the teeth. They were examined morphologically as well as histologically, by using a stereoscopic microscope, SEM, a light microscope and a microradiogram. The results obtained were as follows: 1. The size of the tooth crown of these teeth was smaller than the normal standard, but the form of tooth crown was normal. 2. Based on streomicroscopic observation, enamel hypoplasia was found on the labial and lingual surfaces in B, and on labial surface only in C. In B, the enamel hypoplasia on labial surface was extended widely to incisal 2/3 of the crown, while that on the lingual surface was minimum. In C, there were two spots of enamel hypoplasia located on the mesial and distal incisal edges respectively. 3. Based on SEM observation, the hypoplastic enamel was formed right after their birth. 5. Based on microradiographic observation, the neonatal lines were radiolucent, and the adjacent areas of the neonatal line did not show any poor calcification. 6. From these histological observations, it was found that the perinatal disturbance directly affected the tooth enamel formation and these enamel hypoplasias were positively caused by the cerebral palsy of these children.

Cerebral Palsy↗
PubMed1986

Purification and immunofluorescence localization of the mutant gene product of a Tetrahymena cdaA1 mutant affecting cell division.

A division arrest mutant, cdaA, of Tetrahymena thermophila is known to have a ts-defect in the formation of the fission zone which determines the position of the fission plane. A protein (Mr = 85,000; pI = 4.7, designated as p85) has recently been identified in our laboratory as a possible gene product of the cdaA locus by two-dimensional gel electrophoresis and genetic experiments (Ohba et al., submitted). In the present research, we have isolated p85, prepared its antibody, and demonstrated that in wild-type cells or in cdaA cells at permissive temperature, immunofluorescence for p85 appears on the equatorial basal bodies at the predicted fission zone just before formation of the zone. In such a case, the fission zone appears to be formed just anterior to the fluorescence-associated basal bodies, and then constriction of the division furrow occurs at the zone. However, in cdaA cells at the restrictive temperature, the equatorial p85 deposit and subsequent fission zone formation and furrowing do not occur at all. Thus, we conclude that p85 plays a key role in the formation of the fission zone and in the positioning of the equatorial fission line.

Animals↗
PubMed1981

Dental care for severely handicapped children.

Dental care for the physically and mentally handicapped ought to be a normal part of the responsibilities of the pedodontist. In the case of severe handicap, however, it may be difficult to achieve an acceptable standard of care under general practice conditions. An account is given of the regimen followed in the Tokyo Metropolitan Rehabilitation Institute for the Severely Handicapped where a team approach to dental care has been in existence for the past 10 years. Data are presented which demonstrate the incidence of dental disease in various categories of handicap and examples are given of the improvement in the quality of life of the institutionalized handicapped as a result of regular, high quality dental care.

Adolescent↗