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Biomedical subjects

I Nolte

Publications and source records attributed to I Nolte.

At least 109 records · Page 6Linked to original sources
PubMed1993

The influence of storage time on the nucleotide concentration of canine platelets.

Nucleotide concentrations of canine platelets during storage for 50 days were determined to find out the variation in the concentration of various nucleotides in canine platelets during storage at +4 degrees C for 50 days. Platelets of 3 dogs were stored in whole blood in PVC bags containing CPDA-1 stabilizer. The bags were kept at 4 degrees C for 50 days. Changes in the energy metabolism of the platelets during storage were determined by successive measurements of the following nucleotides: adenosine-diphosphate (ADP), adenosine-tri-phosphate (ATP) and guanosine-tri-phosphate (GTP). In preparation for the measurement of their nucleotide content, the platelets were isolated from the blood at regular intervals and their membranes destroyed by deep freezing, thawing, double sonification in ice and protein precipitation with perchloric acid. The concentration of nucleotides within the platelets was determined by "High Pressure Liquid Chromatography" (HPLC). ADP, ATP and GTP were identified in the peak areas of the HPLC using 3 different techniques: comparison of retention time of standard nucleotide solutions as control samples, enzyme tests and thin layer chromatography. Adenosine-mono-phosphate (AMP), adenosine, hypoxanthine and inosine mono 5'-phosphate (IMP) were not found in dog platelets. Platelet ATP concentration decreased significantly during 50 days of storage (p < 0.001), and was reduced to 24% of the concentration measured after 1 hour of storage on day 50. ADP concentration also dropped significantly during 50 days of storage (p < 0.001). On day 50 the average ADP concentration was reduced to about 34% of the concentration measured after 1 hour of storage.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1993

Investigations on dietary treatment of struvite urolithiasis: 3. Effect of an acidifying diet on acid-base- and mineral-balance of cats after acute urethral obstruction.

The effect of a commercial acidifying diet on acid-base-balance and urinary mineral concentration was compared in five healthy cats and five cats with urethral obstruction for six days after the removal of lower urinary tract obstruction. There were no significant differences in urine pH throughout the experiment between the groups. Blood pH was decreased in obstructed animals at the time of presentation at the clinic, but thereafter it did not differ from data from healthy cats on the same diet. The concentration of phosphorus and potassium in urine decreased, sodium and chloride did not change, while magnesium and calcium content increased.

Acid-Base Equilibrium↗
PubMed1992

[Laboratory diagnosis and differential diagnosis of disseminated intravascular coagulation in the dog].

The laboratory diagnostic possibilities for characterization of disseminated intravascular coagulation (DIC) in dogs are reviewed. A DIC can be demonstrated by means of: 1. Simultaneous consumption of platelets, fibrinogen, coagulation factors and inhibitors of coagulation 2. Increased plasma levels of the specific reaction products fibrin monomers and fibrinopeptides 3. Secondary hyperfibrinolysis, especially an increase in plasma concentrations of fibrin(ogen) degradation products 4. Presence of schistocytes in the blood smear 5. Improvement in the coagulation values during an efficacious anticoagulant therapy 6. Indication of enhanced turnover and formation of microthrombi in different organs by radioisotopically detected coagulation components. Different principles of the laboratory diagnostic procedure in DIC are illustrated by the results of an animal experiment and clinical case reports. Concerning differential diagnosis among other things a deprivation- and dilution effect as well as a disturbance of synthesis have to be considered. One must especially bear in mind that a DIC may also be caused by different coagulator disturbances, e.g. of prothrombin complex synthesis. A DIC can mask such a defect in laboratory diagnostic findings, thereby complicating the diagnosis.

Animals↗
PubMed1992

[The therapy of chronic diseases of the respiratory tract in dogs and cats].

Chronic conditions of the respiratory system are a diagnostic as well as a therapeutic challenge to the veterinarian. In the majority of cases the protective structures, e.g. the vibrating epithelium, have already been irreversibly damaged, so that therapy can only relieve the trouble, but not lead to a full recovery. Prior to initiating a long-term therapy that requires a lot of patience from the veterinarian as well as the owner, it is therefore crucial to carry out a thorough diagnostic procedure. This is a review of the pathophysiological basis and the resulting diagnostic consequences of chronic diseases of the respiratory system. A few selected cases are discussed and recommendations for medical treatment are given.

Animals↗
PubMed1992

[The pathogenesis of the "endometritis-pyometra complex" in the female dog].

Based on investigations in 51 bitches (uteri and ovaries after ovariohysterectomy), suffering from "endometritis-pyometra-complex" (without, n = 38; with, n = 13, hormonal pretreatment), morphologic-functional endometrial characteristics (light microscopy, scanning electron microscopy, enzyme histochemistry, immunohistochemical estrogen receptor analysis) are correlated with ovarian findings, plasma estradiol and progesterone levels as well as microbiological results. The estrous phase of all cycling patients was determined as "diestrus". Plasma estradiol and progesterone concentrations fluctuate within physiological variations. In all cases, not including those, pretreated with progestagen, histological signs of irregular hyperproliferation of uterine glands are obvious, closely related with enzyme histochemical aberrations and atypical endometrial estrogen receptor state. This is interpreted as indicative for prolonged periestrous estrogen induced effects, undergoing different stages of secretory transformation due to the species specific long lasting corpus luteum period. A comparable pathogenesis is supposed deriving from estrogen applications (e. a. after mismating) due to intensified hormonal stimulation and sensibilization of the endometrium, predisposing to ascendant infections by facultative pathogenic bacteria (e. a. E. coli, Streptococcus sp.). Patients without ovarian cyclicity, resulting from progestagen application, exhibit findings, that indicate hormonal imbalances: secretory hypertrophy, irregular regeneration, fibrous atrophy and rigid secretion, resembling those possibly occurring in women after long administration of oral contraceptives.

Animals↗
PubMed1991

[Modification of the global coagulation tests for the dog. Significance for the monitoring of heparin therapy].

When the global coagulation tests Quick's test, aPTT and thrombin time are carried out on dogs using reagents from the human medical sector according to the manufacturer's instructions, they react insensitively to deficiencies of the corresponding coagulation factors or to the presence of fibrin degradation products. In the present study it was attempted to develop sensitive global tests for the dog by modifying these tests. The Quick's test reacted apparently more sensitively after a 1:4 dilution of the test plasma with physiological saline solution. The sensitivity of the aPTT was demonstrated by the addition of BaSO4-adsorbed plasma to normal plasma. It seems to be particularly advantageous to combine a 1:3 plasma dilution with a reduction in the CaCl2-concentration to 5 mmol/l. Likewise, plasma dilution allows the thrombin time to react more sensitively in the presence of increased concentrations of fibrin degradation products. The aPTT and thrombin time are, as in human beings, suited for the control of a heparin therapy. However, since prolongation of the coagulation times is considerably dependent on the test chosen, the heparin sensitivity of the test should be determined in vitro prior to use.

Animals↗
PubMed1991

[Thrombocyte aggregation in the cat].

Platelet aggregation in healthy and sick cats after adding various aggregating agents is described. Feline platelets aggregate irreversibly in response to 0.15-1.0 micrograms/ml collagen, 1 microM ADP, 0.3 IU/ml test-thrombin and 0.71 NIH/ml Topostasin. Epinephrine, ristocetin and kaolin failed to cause aggregation. The aggregation function was decreased in a cat with liver damage and icterus; in 2 cats with uremia platelet aggregation was normal. Acetylsalicylic acid (ASA) (10 mg/kg iv) inhibits platelet aggregation in the presence of collagen in low concentrations; high concentrations of collagen succeeded in inducing platelet aggregation.

Adenosine Diphosphate↗
PubMed1990

Alterations of IgE immunoglobulin concentration in the serum after megavoltage radiotherapy with large-field technique in patients with Hodgkin's disease.

In patients with Hodgkin's disease, the serum IgE concentrations were determined with the PRIST radioimmunoassay before commencing and after ending systemic megavoltage radiotherapy. 57 patients showed mainly raised serum IgE concentrations before radiotherapy. After the end of radiotherapy, 44 patients were investigated, and a highly significant fall of the IgE concentration was shown in the serum following the radiotherapy. The decrease of IgE concentration was most pronounced in the patients in whom large areas of irradiation were necessary or who displayed B symptoms. In a proportion of the patients, the IgE concentration in the serum could be measured once more six and twelve months after the end of radiotherapy. Although the IgE concentrations rose again after radiotherapy, the initial values before the beginning of radiotherapy had not yet been reached again even twelve months after treatment in 21 patients investigated. A long-lasting immunosuppressant effect of radiotherapy on the serum IgE concentration could thus be demonstrated which corresponds to the effect on the serum IgM concentration described earlier.

Follow-Up Studies↗
PubMed1989

Sonographic changes of the pancreas in chronic renal failure.

Several abnormalities regarding pancreatic morphology and function have been reported in patients with chronic renal failure (CRF) with an incidence as high as 72%. In a prospective study we investigated 96 outpatients from our chronic ambulatory hemodialysis program by abdominal ultrasound. Of the patients with CRF, 20.6% were found to have morphologic alterations of the pancreas compared to 4.7% of controls. Although pathologic sonograms of the pancreas correlated with biliary disease, hyperparathyroidism and years of hemodialysis, the most obvious etiologic factor appeared to be the duration of CRF. Possible pathogenetic mechanisms are discussed and screening abdominal ultrasound examinations in patients with long-standing CRF are recommended.

Adult↗
PubMed1988

[Antibiotic treatment of diarrhea in dogs and cats].

The most important reason for diarrhea in dogs and cats are dietary errors. In addition there is a multitude of other influences which may lead to diarrhea. Bacteria play a minor role in diarrhea-associated disease. The so-called primary bacterial intestinal infections occur rarely as it is shown in the examinations presented. Except for the situation in which a specific bacterial activator is the cause of continuous diarrhea in general the administration of antibiotics is ineffective. The treatment of diarrhea should rather consist of food deprivation, oral or parenteral rehydration, use of antisecretory medicaments and of adsorbents, diet and, in case of spasms, administration of anti-cholinergics.

Animals↗
PubMed1988

[Changes in isoagglutinin titers after high-voltage therapy with the large-field technic in Hodgkin's disease patients].

In 48 patients with Hodgkin's disease, the naturally occurring isoagglutinins anti A1, anti A2 and anti B were investigated and their titer determined before commencement of radiotherapy as well as after the end of radiotherapy, and in a proportion of the patients six and twelve months after the end of radiotherapy. Radiotherapy resulted in a highly significant fall in the isoagglutinin titer amounting on average to one titer level compared to the initial value before radiotherapy. After the end of radiotherapy, there was no further fall of the isoagglutinin titers, but a marked reduction of the isoagglutinin titers was still shown six and twelve months later. A long lasting reduction of serum immunoglobulins after radiotherapy in patients with Hodgkin's disease is known from earlier investigations; this applies in particular to IgM. Since the isoagglutinins mainly belong to the IgM fraction, our finding of persistent reduction of isoagglutinin titers after radiotherapy correlates with the known long-lasting reduction of serum IgM concentration.

ABO Blood-Group System↗
PubMed1988

[The progress of a fibrinolytic therapy with streptokinase, urokinase and warfarin in a thoroughbred with intermittent lameness with special reference to resonance thrombography].

A thoroughbred horse, suffering from intermittent lameness was treated with streptokinase, urokinase and warfarin. The appearing fibrinolytic changes in coagulation were exhibited in a resonance thrombogram. In attendant coagulation studies fibrinogen- and fibrin-degradation products were demonstrated. The stallion does not show any lameness since the end of the treatment and is standing in a breeding station since March 1988.

Animals↗
PubMed1988

[The effectiveness of the combined treatment of thrombocytic hemorrhagic diathesis of dogs with prednisolone and blood transfusion in the model of an aspirin-produced thrombocytopathy].

The administration of 20 mg/kg of acetylsalicylic acid in 18 clinically healthy dogs resulted in a thrombocytopathy with lengthened capillary bleeding time and irreversible aggregation inhibition. Through the set up of individual dilution series, one could conclude the proportional percentage of aggregation functional transfused thrombocytes. The capillary bleeding time was shortened after the intravenous injection of prednisolone (5 mg/kg) without measurable influence on the thrombocytes. Compared to the singular use of cortisone or blood transfusion alone, the effect on capillary bleeding time became magnified when one combined transfusion and corticosteroids. The fresh blood conserves (12 hours) were, with respect to the haemostyptical properties, superior to blood stored for 5 days. The component of aggregation-functional thrombocytes was, due to the prophylactic cortisone application prior to transfusion, not increased.

Animals↗