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Biomedical subjects

I Miyoshi

Publications and source records attributed to I Miyoshi.

At least 307 records · Page 17Linked to original sources
PubMed1982

Characteristics of a brain lymphoma cell line derived from primary intracranial lymphoma.

A brain lymphoma cell line, designated TK, was established from biopsy material of a patient with primary intracranial lymphoma. The TK cell line grew in suspension with clumps of cells and consisted of primitive lymphoid cells. TK cells and original lymphoma cells from which the cell line was derived bore surface immunoglobulin and lacked Epstein-Barr virus-determined nuclear antigen. Transplantation of the TK cells into immunosuppressed newborn hamsters gave rise to invasive growth of tumors with metastasis to the brain and eyes. These findings provide definitive evidence for a B-cell origin of the brain lymphoma in this case.

Adult↗
PubMed1982

Isolation and characterization of retrovirus from cell lines of human adult T-cell leukemia and its implication in the disease.

A retrovirus (ATLV) was unequivocally demonstrated in human adult T-cell leukemia (ATL) cell lines by density (1.152-1.155 g/cm3) in a sucrose gradient, reverse transcriptase activity insensitive to actinomycin D, RNA labeled with [3H]uridine, and specific proteins with molecular weights of 11,000, 14,000, 17,000, 24,000, and 45,000. Furthermore, cDNA prepared by endogenous reaction with detergent-treated virions hybridized to 35S RNA containing poly(A), which was inducible by IdUrd treatment of a T-cell line derived from leukemic cells of the ATL, and the integrated form of ATLV proviral DNA was detected in T-cell lines derived from ATL. The ATLV proviral DNA was also detected in fresh peripheral lymphocytes from all five patients with ATL tested so far but not in those from healthy adults. On the other hand, ATLV protein of Mr 42,000 was found to be at least one of the ATL-associated antigen(s) that were previously detected in ATL-leukemic cells by all sera from patients with ATL. These findings on the close association of ATLV protein and proviral DNA with ATL are direct evidence for the possible involvement of the retrovirus ATLV in leukemogenesis of human ATL.

Adult↗
PubMed1982

Avian antisera to various gangliosides: detection by enzyme immunoassay.

We attempted to produce specific antibodies to various gangliosides by immunizing chickens. Antibody activity was determined by enzyme immunoassay (EIA). The optimal conditions for EIA were examined by using chicken anti-GM1 ganglioside (GM1) serum and the final procedure was as follows. Fifty microliters of ethanol solution containing 2.5 micrograms of the glycolipid antigen and 50 micrograms of taurodeoxycholate were added to each well of an EIA microtitration plate and dried at 37 degrees C for 2 h. Nonspecific sites were blocked by incubation with 1% gelatin-containing buffer, then titration of the chicken antisera was carried out in the antigen-coated plate by incubation at 4 degrees C for 12 h. Next, alkaline phosphatase-labeled anti-chicken IgG (specific antibody, 15 ng; enzyme, 0.054 units) was allowed to react at 37 degrees C for 2 h. The enzyme activity which was bound to the plate was assayed with p-nitrophenol phosphate as substrate. Under the above conditions, anti-GM1 serum reacted strongly with GM1 and asialo GM1, and anti-GM2 serum indicated a considerable specificity for GM2. However, we failed to elicit any antibody to GD1a, GD1b, and GT1b. Anti-NeuAc-sialosylparagloboside and anti-NeuGc-sialosylparagloboside sera showed a high specificity for the homologous ganglioside. However, anti-NeuGc-hematoside serum reacted equally with both the homologous antigen and NeuGc-sialosylparagloboside, and anti-NeuAc-hematoside (GM3) serum cross-reacted with both N-acetyl and N-glycolyl types of hematoside and sialosylparagloboside.

Animals↗
PubMed1982

Characterization of xenoantiserum produced against B cell acute lymphoblastic leukemia cell line.

Antiserum was produced in white rabbit by intravenously injecting living cells of a B cell acute lymphoblastic leukemia (ALL) line (BALL-1). The reactivity of the antiserum against various lymphoid cell lines was examined by membrane immunofluorescence after appropriate absorption. Serum absorbed with non-T, non-B (NALL-1) and T-ALL (TALL-1) cells recognized B cell antigens distinct from Ia-like antigens on both normal and neoplastic B cells. After further absorption with tonsillar cells or normal B cell line (KO-HL-3), it reacted only with BALL-1 cells and did not react with other leukemia/lymphoma and normal B cell lines. The serum absorbed with tonsillar cells reacted only with BALL-1 and some B cell lines. Thus we were able to obtain antisera with specificity to B cell antigen, B-ALL antigen, and B cell line antigen.

Animals↗
PubMed1982

Detection of type C virus particles in Japanese monkeys seropositive to adult T-cell leukemia-associated antigens.

Type C virus particles which were identical to adult T-cell leukemia virus (ATLV) in morphology were detected in phytohemagglutinin-stimulated peripheral lymphocytes from four out of four Japanese monkeys seropositive to adult T-cell leukemia-associated antigens. It is possible that non-human primates may be a natural reservoir of ATLV, and vector-borne transmission of ATLV from monkeys to humans should be explored.

Animals↗
PubMed1982

A retrovirus from human leukemia cell lines: its isolation, characterization, and implication in human adult T-cell leukemia (ATL).

We have tried to isolate a retrovirus from adult T-cell leukemia (ATL) which is a new clinical entity of T-cell malignancy. This disease shows a peculiar geographic clustering of patient birthplaces in the southwestern part of Japan. A retrovirus was isolated from the T-cell line, MT-2, which was established from cord lymphocytes cocultivated with leukemic cells from an ATL patient and characterized by: (a) density of 1.152-1.155 g/ml in sucrose gradient; (b) reverse transcriptase activity; (c) specific protein components; (d) RNA labeled with 3H-uridine, and (e) specific DNA complementary with viral RNA. The retrovirus was named adult T-cell leukemia virus (ATLV). Complementary DNA (cDNA) prepared by the endogenous reaction of detergent-treated virions hybridized with 35S RNA in MT-2 cells and another ATL cell line, MT-1, and this 35S RNA was inducible with IUDR treatment of the MT-1 cells, indicating that ATLV is a typical retrovirus containing 35S RNA as the genome. However, the cDNA did not show any detectable hybridization with cellular RNA of other human cell lines unrelated to ATL. The ATLV proviral DNA was detected in the chromosomal DNA of MT-1 and MT-2 cell lines as well well as in fresh peripheral blood cells of all five patients with ATL tested; however, it was not found in those of three healthy adults. Furthermore, sera from the patients reacted with one component of the ATLV protein, but normal sera did not. These sera and all other sera from ATL patients were previously shown to react with antigen(s) in leukemic cells of ATL, and the antigen(s) also reacted with sera from about 25% of the healthy adults in the endemic area, but not in the non-endemic area. These close associations of ATLV proviral DNA and proteins with ATL are direct evidence strongly suggesting the involvement of the retrovirus, ATLV, in the leukemogenesis of human ATL.

Adult↗
PubMed1982

Necessity of bile for and lack of inhibitory effect of retinoid on development of forestomach papillomas in nontreated mutant mice of the W/Wv genotype.

The pathogenesis of papillomas which developed spontaneously in the forestomach of WB X C57BL/6 F1-W/Wv mutant mice was investigated. The thickness of the forestomach epithelium was used as a quantitative index for development of papillomas. From the 15th day after birth, the forestomach epithelium of the W/Wv mice was significantly thicker than that of the congenic +/+ mice. Administration of aromatic retinoic acid analog (ethyl all-trans-9-(4-methoxy-2,3,6-trimethylphenyl)-3,7-dimethyl-3,7-dimethyl-2,4,6,8 -nonatetraenoate) did not suppress development of papillomas. Since papillomas did not appear in the stomach which was removed from the W/Wv embryos and grafted to the s.c. space produced in the back of the adult mice and since a considerable amount of bile reflux preceded development of papillomas, bile reflux may be a cause of papillomas in W/Wv mice.

Alleles↗
PubMed1981

Hairy cell leukemia: establishment of a cell line and its characteristics.

A hairy cell leukemia (HCL) line, ZK-H, was established from peripheral blood of a 69-year-old male patient. The ZK-H cells and the patient's original hairy cells shared the same surface properties; both possessed membrane-bound IgG with kappa light chains and villous surface structures. The ZK-H line carried Epstein-Barr virus (EBV)-determined nuclear antigen, but the patient's fresh leukemic cells lacked this antigen. Morphologically, the ZK-H cells appeared lymphoblastoid and more primitive than the preculture cells. The ZK-H line had a hyperdiploid chromosome constitution of 47 and trisomy no. 2. The presence of membrane-bound immunoglobulin and of B-cell tropic EBV in this cell line provides further evidence for the B-cell nature of HCL in this patient

Aged↗
PubMed1981

Chromosome 14q+ in adult T-cell leukemia.

Cytogenetic studies were performed on leukemic cells from two patients with adult T-cell leukemia. A 14q+ marker chromosome was found in the peripheral blood leukocytes from patient No. 1 and in a leukemic T-cell line (MT-1) derived from the peripheral blood of patient No. 2. The 14q+ resulted from a t(12;14) in patient No. 1 and from a t(Y;14) in patient No. 2 with a break point at 14q32 in each case. In addition, the leukemic cells from patient No. 1 showed a t(1;7) and a 9q-, while the MT-1 line had numerous structural abnormalities. Thus, it is clear that a 14q+ translocation is not restricted to B-cell neoplasms but occurs in T-cell neoplasms as well.

Adult↗
PubMed1981

Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera.

Indirect immunofluorescence of certain human sera demonstrated an antigen(s) in the cytoplasm of 1--5% of the cells of a T-cell line, MT-1, from a patient with adult T-cell leukemia (ATL), which is endemic in southwestern Japan. The antigen was not detected in other human lymphoid cell lines, including six T-cell lines, seven B-cell lines, and four non-T non-B cell lines. The antigen did not show cross antigenicity with that of herpesviruses, including Epstein--Barr virus, herpes simplex virus, cytomegalovirus, varicella-zoster virus, herpesvirus saimiri, and Marek disease virus. The proportion of antigen-bearing cells was increased by a factor of approximately 5 on culture in the presence of 5-iodo-2'-deoxyuridine. Antibodies against the antigen in MT-1 cells were found in all 44 patients with ATL examined and in 32 of 40 patients with malignant T-cell lymphomas (most of them had diseases similar to ATL except that leukemic cells were not found in the peripheral blood). The antibodies were also detected in 26% of the healthy adults examined from ATL-endemic areas but in only a few of those examined from ATL-non-endemic areas. On electron microscopy, extracellular type C virus particles were detected in pelleted MT-1 cells cultured in the presence of 5-iodo-2'-deoxyuridine.

Adult↗
PubMed1981

A T-cell line derived from normal human cord leukocytes by co-culturing with human leukemic T-cells.

A unique T-cell line, MT-2, was established from normal human cord leukocytes of a male infant by co-culturing with leukemic T-cells from a female patient with adult T-cell leukemia. MT-2 cells expressed receptors for sheep erythrocytes and complement and were reactive with anti-T-cell and anti-Ia sera. They were negative for Fc receptors, surface immunoglobulin, and Epstein-Barr virus nuclear antigen. Chromosomally, the MT-2 line was male and most cells were shown to have a normal diploid karyotype. The cultured cells were tumorigenic when transplanted into immunosuppressed newborn hamsters.

Animals↗