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Biomedical subjects

I Miyoshi

Publications and source records attributed to I Miyoshi.

At least 289 records · Page 16Linked to original sources
PubMed1983

Transformation of Bloom syndrome T-lymphocytes by cocultivation with a lethally irradiated human T-cell line carrying type C virus particles.

The present study describes the establishment of T-cell lines from the peripheral blood of two Bloom syndrome (BS) patients and one healthy female by co-cultivation with a lethally irradiated human T-cell line (MT-2) carrying adult T-cell leukemia (ATL) virus (ATLV). The cell lines from normal and BS subjects exhibited cell surface markers compatible with T-cell origin, and BS T-cell lines retained the original cytogenetic characteristics of the syndrome. Even though phytohemagglutinin-stimulated BS lymphocytes from the two BS patients studied all showed high levels of sister chromatid exchange (SCE), one of the BS T-lines retained the high SCE level in 100% of the cells and the other BS T-line contained two populations, one with high SCE (70%) and the other with normal SCE (30%), at a relatively constant frequency over 6 months. Transformation of normal and BS cells by cocultivation with MT-2, which carries ATLV, did not cause karyotypic changes over 6 months. ATLV infection, chromosome instability, karyotypic abnormality and SCE in BS T-lymphocytes are also discussed.

Bloom Syndrome↗
PubMed1983

Japanese Burkitt's lymphoma: clinicopathological review of 14 cases.

The clinicopathological features of 14 Japanese patients with Burkitt's lymphoma (seven studied by us and seven reported by others) are reviewed. Five patients were 12 years of age or younger. Six patients presented with abdominal masses and five with jaw tumors. Twelve patients manifested blood and/or marrow involvement at the time of initial diagnosis (four patients) or later in the course of the disease (eight patients). Most of the patients had a rapid clinical course in spite of combination chemotherapy. The malignant cells from all patients exhibited a B-cell phenotype; the surface immunoglobulin was IgM in 13 and IgG in one. Two patients had tumors positive for Epstein-Barr virus-determined nuclear antigen while all the others lacked this antigen in their tumors. Chromosome analysis revealed a standard t(8;14) translocation in nine patients, a 14q+ chromosome without an identifiable donor chromosome in two and variant translocations t(2;8) in two and t(8;22) in one.

Adolescent↗
PubMed1983

[Establishment and characterization of ATLV-producing cell lines].

Peripheral blood lymphocytes from a Japanese monkey (Macaca fuscata) were co-cultivated with lethally irradiated MT-2 cells that carry abundant type C virus particles (ATLV) isolated from a patient with adult T-cell leukemia (ATL). After six weeks, a lymphoid cell line designated Si-1 was established from the simian lymphocytes. The Si-1 line was E-, SIg-, Leu-1-, OKI1+, EBNA-, ATLA+ and ATLV+. Co-cultivation of peripheral blood lymphocytes from three anti-ATLA positive and two negative healthy adults resulted in the establishment of three lymphoid cell lines derived either from an anti-ATLA positive donor or both donors. All three cell lines were E+, SIg-, Leu-1+, OKI1+, EBNA-, ATLA+ and ATLV+. This mixed lymphocyte culture technique provides a simple means for the isolation of ATLV from healthy ATLV carriers.

Adult↗
PubMed1983

Three cases of pre-adult T-cell leukemia.

Three patients with pre-adult T-cell leukemia (ATL) are reported. All of them were seropositive to ATL-associated antigens (ATLA) and had 3-30% abnormal lymphocytes with cleaved or lobulated nuclei in the peripheral blood in the absence of clinical symptoms of ATL. All the data on immunological tests were normal except for negative purified protein derivative skin tests. In Case 1 the patient was an elder sister of an ATL patient. Four other members of this family were also anti-ATLA positive without abnormal cells in the blood. In Cases 1 and 2, ATLA and ATL virus (ATLV) were expressed in the lymphocytes cultured with phytohemagglutinin or T-cell growth factor. Moreover, an ATLV-producing cell line was established from Case 1 by mixed lymphocyte culture with lymphocytes obtained from a normal anti-ATLA negative person. Investigation of pre-ATL cases may clarify factors leading to the development of ATL in ATLV-infected persons.

Adult↗
PubMed1982

A new subtype of human T-cell leukemia virus (HTLV-II) associated with a T-cell variant of hairy cell leukemia.

Human T-cell leukemia virus (HTLV) is a human type-C RNA tumor virus (retrovirus) previously identified in and isolated from several patients with T-cell leukemias or lymphomas. The known virus isolates from the United States and Japan are closely related and are found in adults with an acute malignancy of mature T cells. A related retrovirus has been found in a patient (Mo) with a somewhat different disease (a T-cell variant of relatively benign hairy cell leukemia). Serum from Mo contains antibodies to the major internal core protein (p24) of HTLV. A T-cell line established from the spleen of Mo expresses HTLV antigens. However, HTLV from Mo is significantly different from all previous HTLV isolates in immunological cross-reactivity tests of p24. The usual prototype HTLV isolate is represented as HTLV-I, and the HTLV from Mo is represented as HTLV-II. Individual members of each subgroup may then be identified by subscript initials of the patient [for example, HTLV-I(CR), HTLV-I(MB), and HTLV-II(Mo)].

Antibodies, Viral↗