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Biomedical subjects

I Mehdi

Publications and source records attributed to I Mehdi.

31 records · Page 2Linked to original sources
PubMed1998

Frequency of gastrointestinal tumours at a teaching hospital in Karachi.

Malignant gastrointestinal tumours are amongst the commonest tumours exhibiting an annual increase globally. There is a change in the morphological site of involvement observed over the years. In this study biopsy proven malignant gastrointestinal tumours seen at Jinnah Postgraduate Medical Centre, Karachi from 1961-1992 were analyzed with reference to age, sex, topography and histology. The study showed an increase in malignant gastrointestinal tumours over the years, from 9% in 1961 to 17% in 1992 with respect to all malignant tumours reported. The tumours affected a much younger age in our population, 74% occurring between 35-64 years of age. Carcinoma oesophagus accounted for 10% of all malignancies (48.7% male and 62.4% female gastrointestinal tumours), while gastric carcinoma remained unchanged (14% male and 9% female GI tumours). The colorectal carcinoma (25.4% of male and 20.1% of female GI tumours) and carcinoma pancreas (1.2% male and 1.5% female GI tumours) were less frequently seen. It was observed that malignant gastrointestinal tumours have increased significantly over the years in our local population as part of international trend and are occurring at a much younger age as compared to western population. Carcinoma oesophagus was seen more frequently than gastric carcinoma and colorectal carcinoma. A substantially higher number tend to be more anaplastic being seen at an advanced stage of disease at the time of diagnosis.

Adolescent↗
PubMed1991

Detection of single-stranded DNA damage using monoclonal anti-thymidine antibody.

A method to detect single-stranded DNA damage from individual cells has been developed using a monoclonal anti-thymidine antibody (MoAb20B7). Initially, HL-60 cells were incubated with daunomycin at different concentrations, and processed by MoAb20B7. While 73.5% of the cells incubated with 5 micrograms/ml of daunomycin for 24 h reacted positively with MoAb20B7, 83.5% cells at 10 micrograms/ml daunomycin dose were positive. Next, this method was combined with unscheduled DNA synthesis to simultaneously measure repair and damage from individual cells. Finally, patients with acute myeloid leukemias were studied before and 24 h after therapy with a daunomycin containing regimen. In vivo damage could be determined in a prompt fashion.

Animals↗
PubMed1989

Detection of BrdU in the regenerating bone marrow cells of a patient with AML.

A patient with acute myeloid leukemia received bromodeoxyuridine (BrdU) prior to remission induction therapy. Prior to therapy only leukemic blast cells were found to be labeled. The marrow was aplastic by day 17 with the marrow cavity being devoid of BrdU labeled cells. In contrast rare BrdU containing cells were noted in the paratrabecular and endosteal regions. As the marrow recovered, many regenerating cells contained BrdU, implying that normal stem cells must have been in S-phase at the time of BrdU infusion, and repopulation of marrow may be accounted for by the activity of only a few clones in remission.

Bone Marrow↗
PubMedDate not supplied

Diagnostic efficacy of stool antigen test (HPSA), CLO test and serology for the detection of Helicobacter pylori infection.

BACKGROUND: The diagnosis of Helicobacter pylori infection was initially being made through invasive methods but now non invasive methods have been developed to make the diagnosis easier. The present study was done to evaluate the diagnostic efficacy of a two non invasive tests i.e. Helicobacter pylori Stool antigen test (HpSA) and Helicobacter pylori IgG serology with an invasive method i.e. Campylobacter like organism (CLO) gel test. METHODS: The study was conducted in the gastroenterology unit of Pakistan Medical Research Council Research Centre Karachi. Adult patients with gastroduodenal disease were selected for study and their medical history was recorded. Endoscopy was done on all patients and the antral biopsy sample was tested for H. pylori using CLO test. Serology (IgG) was done elsewhere using ELISA and titers of over 50 units were recorded as positive. HpSA was done to determine the presence of H. pylori antigen in stool. RESULTS: Out of 43 patients 34 (79%) were males and 9 (21%) females. The main presenting symptom was epigastric pain in 74% cases. Although H. pylori IgG antibody titers of over 50 were taken as positive but for this study titres of over 100 were taken as significant for comparison with other tests. CLO test was positive in 26 (60.5%) cases, H. Pylori antibody titers of over 100 IU were present in 33 (76.7%) cases and HpSA in 21 (48.8%). Using CLO test as the gold standard the sensitivity of serology was 81% and that of HpSA 65% with a 29% and 76% specificity respectively. CONCLUSION: In our setting CLO test is still the best diagnostic test for H. Pylori detection. Both non invasive tests i.e. serology and stool HpSA are less sensitive than CLO but amongst each other both are equally sensitive.

Adult↗
PubMedDate not supplied

DNA damage/repair studies using a combination of monoclonal thymidine antibody and unscheduled DNA synthesis simultaneously.

The ability of a new monoclonal antibody against thymidine (MoAb 20B7) to detect chemotherapy induced single stranded DNA damage is described. HL-60 cells were used for these experiments. Damage to DNA was caused by incubation of cells with alkylating agents. Portions of DNA which is damaged are cleaved by cellular endonucleases, thus exposing thymidine on the opposite DNA strand. Processing of these samples by MoAb 20B7 showed that such damaged segments could be detected consistently. Furthermore, this method was combined with autoradiographic detection of unscheduled DNA synthesis thereby allowing for assessment of DNA repair simultaneously from the same cell.

Antibodies, Monoclonal↗