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Biomedical subjects

I Mano

Publications and source records attributed to I Mano.

At least 19 recordsLinked to original sources
PubMed1992

Organization and expression of the gene encoding chick kainate binding protein, a member of the glutamate receptor family.

The gene encoding chick cerebellar Bergmann glia-specific kainate binding protein (chKBP), has been isolated, characterized and expressed in heterologous systems. The structural gene spans 11.2 kb and contains 11 exons and 10 introns. Several of the exons encode specific receptor domains, including each of the predicted transmembrane regions. Exon/intron boundaries flanking the second, putative channel-forming transmembrane domain are conserved between chKBP and other glutamate/kainate receptor subunits. The putative promoter region 5' to the first exon displays high GC content and TATA, CAAT and AP1 consensus sequences. Transcription of the chKBP gene is evident prior to full cerebellar cortical maturation. Transcripts are abundant in cells consistent with Bergmann glia, as revealed by in situ hybridization. Transfection of 293 kidney cell cultures with chKBP cDNA or chKBP gene expression constructs confers CNQX-sensitive kainate binding with the pharmacological specificity displayed by both chKBP and kainate receptors. However, expression of the same constructs in Xenopus oocytes fails to yield detectable agonist-activated currents.

Amino Acid Sequence↗
PubMed1990

[Super conductive MR imaging of small acoustic tumor].

Eight patients with acoustic tumor were studied with a superconductive MR imaging. T1-weighted images with Gd-DTPA most accurately showed the margin of the seventh and eighth nerves in the internal auditory canal and were most sensitive in detecting small tumors. T2-weighted images were inferior to T1-weighted images, that could fail to detect small tumors. High resolution, thin slice, MR imaging using surface coil is particularly useful for small acoustic tumor because of its higher specificity compared with air-CT, cisternography.

Adult↗
PubMed1989

Molecular structure of the chick cerebellar kainate-binding subunit of a putative glutamate receptor.

Kainate receptors mediate some of the excitatory transactions carried out in the central nervous system by the neurotransmitter glutamate. They are involved in neurotoxicity, possibly in neurodegenerative disorders and it has been suggested that they have a role in long-term potentiation. Kainate receptors are present both on neuronal and glial cell membranes where they regulate the gating of a voltage-independent ion channel. Nothing is known about their molecular structure. Taking advantage of the unusually high abundance of 3H-kainate binding sites in the chick cerebellum, we have isolated an oligomeric protein that displays a pharmacological profile similar to that of a kainate receptor, and have demonstrated, using the monoclonal antibody IX-50, that this protein is composed of a single polypeptide of Mr 49,000 which harbours the specific kainate recognition site. The structure of this kainate binding protein (KBP) is also of interest because of its exclusive cerebellar localization on Bergmann glial membrane in close proximity to established glutamatergic synapses. We now report the isolation of the complementary DNA containing the complete coding region of the kainate binding protein. The predicted structure of the mature protein has four putative transmembrane domains with a topology analogous to that found in the superfamily of ligand-gated ion channels. This raises the possibility, that kainate binding protein may form part of an ion channel and may be a subunit of a kainate subtype of glutamate receptor.

Amino Acid Sequence↗
PubMed1989

[Magnetic resonance imaging in 38 cases of acoustic tumors].

The value of magnetic resonance imaging (MRI) in the diagnosis of acoustic tumors was retrospectively assessed in 38 cases. A 0.15 Tesla permanent magnet and a 1.5 Tesla superconducting magnet were employed in 24 and 14 cases, respectively. Gadolinium diethylene triamine pentaacetic acid (Gd-DTPA), a paramagnetic contrast agent, was used in 10 cases. Acoustic tumors were identified in all cases. Small, medium, and large tumors were depicted with equal clarity by MRI and computed tomography (CT). However, tumor contour and extension, accompanying cysts, and brainstem displacement were more clearly visualized on MRI. The use of Gd-DTPA improved the quality of the MR images by markedly enhancing the acoustic tumors in all cases. In particular, detection of small acoustic tumors and intra- or paratumoral cysts was facilitated by the use of Gd-DTPA. The possibility of a correlation between acoustic tumor histology and MRI features was studied by calculation of the contrast to noise (C/N) ratio in 10 cases of acoustic tumor and 7 cases of meningioma. No definite correlation was demonstrated, but there appeared to be some difference in the C/N ratio between acoustic tumors and meningiomas. In three volunteers, MRI demonstrated intracanalicular nerves, separately. Because of its higher resolution, MRI can be expected to replace CT and air CT in the diagnosis of acoustic tumors.

Adult↗
PubMed1988

A research method for MRI tissue characterization studies using small coils for excised organs.

The main purpose of this report is to describe our method of MRI tissue characterization studies, which consists of MR imaging of the patient, precision MR imaging of the excised organ, and pathological examination of the organ. We first made small coils to be used with conventional MRI scanners to examine the excised organs. In order to make it easier to understand the effectiveness of our method, three illustrative case reports of abdominal or pelvic tumors were presented as examples of the tissue characterization studies. In conclusion, this method seemed very useful and, in addition, it was found that this small coil technique has several advantages in other applications.

Abdominal Neoplasms↗
PubMed1986

New polyvinyl alcohol gel material for MRI phantoms.

Nambu PVA gel which is produced by repeated freezing and thawing of PVA solution has overcome almost all of the problems which present substances have: It is close to human soft tissue in MRI parameters. MRI parameters (1H density, T1, T2) are adjustable to some extent. It has appropriate physical characteristics. The important problem with PVA gel is long-term stability. It is assumed that this problem can be solved by its periodic calibration and replacement.

Cold Temperature↗
PubMed1980

[CT scanner].

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Humans↗