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Biomedical subjects

I Kondo

Publications and source records attributed to I Kondo.

At least 253 records · Page 14Linked to original sources
PubMed1984

Ultrasonography and brain pathology of periventricular hemorrhage and subependymal cyst in the preterm neonate.

Ultrasound (US) findings in 32 premature infants with intraventricular hemorrhages (IVH, 21 cases), subependymal hemorrhages (SEH, 5 cases) or subependymal cysts (SEC, 6 cases) were reviewed to determine the accuracy of US diagnosis and clarify the pathogenesis of SEC. With single US there was the possibility of false-positive or -negative SEH or IVH, but serial US showed diagnostic accuracy for SEH or IVH. Four of 6 cases with iron-negative SEC did not show an image of prior SEH and the SEC suggested focal ischemic microcirculation which may be important in the pathogenesis of the occurrence of SEH or the development from SEH to IVH.

Brain↗
PubMed1984

Isolation and virulence of a caseinase- and bound coagulase-deficient mutant of Staphylococcus aureus BB.

A caseinase - and bound coagulase-negative mutant was isolated from Staphylococcus aureus BB by treatment with N-methyl-N'-nitro-N-nitrosoguanidine. The virulence of the mutant strain was compared with that of the parental strain in mice infected by the intravenous and intraperitoneal routes. The ratio between the LD50 of the mutant and that of the parent was 1,841 for intravenous infection and 106 for intraperitoneal infection. Viable staphylococci were counted in renal tissue three days after intravenous infection with 3 X 10(6) bacteria; the count was 8 X 10(7) cfu/100 mg of tissue for the parental strain and 1.2 X 10(2) cfu/100 mg for the mutant strain.

Abscess↗
PubMed1984

Ring chromosome 10 syndrome: case report and the possibility of clinical diagnosis.

A 2-year-old boy, whose clinical abnormalities included growth retardation(3rd percentile), mild mental retardation (DQ = 67), microcephaly, hypertelorism, strabismus, prominent nasal bridge, stubby nose, pointed chin, undescended testes and hydronephrosis, had a ring chromosome 10. Using high-resolution banding technique, the breakpoints were decided at p15.3 and q26.3 of chromosome 10. The clinical features observed in this case were common in 8 other cases with ring chromosome 10 previously reported. Thus, the terminal deletion of both arms of chromosome 10 seems to be the cause of the specific clinical abnormalities. When a patient has the clinical common abnormalities above mentioned, ring chromosome 10 syndrome will be suspected before chromosome analysis.

Child, Preschool↗
PubMed1984

Megakaryoblastic transformation of chronic myelogenous leukaemia in a child.

A child with megakaryoblastic transformation of Ph1-positive chronic myelogenous leukaemia, complicated by myelofibrosis, is reported. The blastic cells were negative for myeloperoxidase by ultrastructural cytochemistry. They had Factor-VIII antigen and platelet glycoprotein but were negative for platelet peroxidase activity. This discrepancy seemed to be due to neoplastic origin of blastic cells.

Antigens↗
PubMed1984

[Two cases of Buschke-Löwenstein tumor].

We report two cases of Buschke-Löwenstein Tumor on 39- and 55-year-old males. Both patients presented with mass and pain of glans penis. Pathological diagnosis of biopsy specimens were condyloma acuminatum in both cases. Bleomycin was not effective. Therefore, tumor resection in the first case and partial penectomy on second case were performed. Pathological examination revealed hyperkeratosis, acanthosis and downward growth of the prickle cell layer. But cellular atypia were not seen. Pathological diagnosis was Buschke-Löwenstein tumor. Our two patients are alive with no evidence of disease after 10 years. Pathological and clinical problems of Buschke-Löwenstein tumor were discussed by review of the literature.

Adult↗
PubMed1983

Cloning and expression of the staphylokinase gene of Staphylococcus aureus in Escherichia coli.

Restriction fragments of DNA from bacteriophage S phi-C of Staphylococcus aureus which carries the gene for staphylokinase, one of the plasminogen activators, were cloned onto plasmid pBR322. Recombinant plasmids carrying the 2.5 kilobase pair segment of S phi-C DNA confer on Escherichia coli cells the capacity to synthesize staphylokinase. The enzyme is synthesized in amounts comparable to that found in S. aureus, and irrespective of the orientation of cloned fragments and their insertion site on pBR322. The active enzyme produced by E. coli cells is preferentially recovered from the periplasmic space and in part excreted into the culture medium. It is indistinguishable from the enzyme produced by S. aureus in molecular weight, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and in antigenicity, as determined by the micro-Ouchterlony precipitation test.

Cloning, Molecular↗
PubMed1983

Effect of highly purified coagulase and culture filtrate on virulence and immunity of a coagulase-negative mutant of staphylococcus aureus BB.

The virulence of the coagulase-deficient mutant BB-Cgl- 1301 (50% lethal dose [LD50] for mice by the intravenous route) was compared with that of its parental strain, Staphylococcus aureus BB. The BB strain produced free coagulase of serotype I, whereas the mutant 1301 did not. Mice were infected with strain 1301, alone or in combination with a highly purified coagulase type I or type II solution, or with concentrated culture filtrates of parent strain BB or mutant strain 1301. The ratios of the LD50S of 1301 and its combinations to that of BB ranged from 34.9 to 461. Combining strain 1301 with a concentrated culture filtrate of BB (BB-CF2.5) was the most effective for enhancement of its virulence. When mice were infected with a combination of strain 1301 and BB-CF2.5, the LD50 of strain 1301 (1.72 mg of cells [wet weight]) was decreased to 0.13 mg (1.3 x 10(8) CFU). This LD50 yielded the smallest ratio, 34.9, as compared with the LD50 of BB (0.00373 mg). In contrast, when the mice subcutaneously immunized with strain 1301 and BB-CF50 were intravenously challenged by strain BB, the LD50 for the immunized mice was 17.4 times the LD50 for the unimmunized control mice (0.0429 mg as compared with 0.00246 mg), indicating that combination was the most effective for enhancement of mouse immunization with strain 1301. However, combining strain 1301 with the highly purified sample of coagulase increased neither the virulence nor the immunizing power of mutant strain 1301.

Animals↗
PubMed1983

Mapping of the human gene for epidermal growth factor receptor (EGFR) on the p13 leads to q22 region of chromosome 7.

We previously reported that the structural gene for epidermal growth factor receptor (EGFR) can be mapped to the p22 leads to qter region of human chromosome 7 (Shimizu et al., 1979, 1980). In the present study, we produced two series of human-mouse cell hybrids by fusing mouse A9 cells that are deficient in EGFR with the human diploid fibroblast lines GM1356, 46,XX,t(1;7)(p34;p13), and GM2068, 46,XX,t(6;7)(q27;q22), both of which possess EGF receptors. Expression of EGF binding ability in the former series of cell hybrids was correlated with the retention of the human translocation chromosome containing the 7p13 leads to qter region, and in the latter series of cell hybrid it was correlated with the retention of the human translocation chromosome containing the 7pter leads to q22 region. Therefore, the EGFR gene can be localized in the p13 leads to q22 region of chromosome 7.

Cell Fusion↗
PubMed1983

[A study of cefoperazone prostatic tissue levels: difference between the gland and capsule].

Prostatic tissue levels of Cefoperazone (CPZ) were determined in 34 patients with prostatic hypertrophy after the intravenous administration of 1 g of CPZ. Prostatic tissue levels of CPZ decreased slowly with time and the elimination half-life was 125 to 171 minutes. The CPZ concentration in the surgical capsule was higher than that in the gland. As compared with the minimal inhibitory concentrations of CPZ, the prostatic tissue levels were considered high enough for the treatment of post-prostatectomy and bacterial prostatitis. Neither the pathological type of prostatic hypertrophy, that of infiltrations, nor the weight of the removed tissue was correlated to the tissue levels of CPZ.

Aged↗
PubMed1983

[Laboratory and clinical studies on latamoxef in the field of obstetrics and gynecology].

Latamoxef (LMOX) is a new antibiotic synthesized by Shionogi Research Laboratory. Chemically LMOX is especially unique with a sulfur atom replacing the oxygen atom in the 1 position of the conventional cephalosporin nucleus, and in addition, this antibiotic has a cephamycin-like structure. The antibacterial activity of LMOX shows high potency against Gram-negative bacteria, but tends to be weak against Gram-positive bacteria. The tissue levels of LMOX in humans after intravenous injection of 1 g were examined. The levels in uterine and adnexa uteri tissue at 1 hour after administration were 25.4 and 27.4 micrograms/g respectively. LMOX was administered to 147 cases in infections of obstetric and gynecological field. The clinical effect according to disease was 94.6% for intrauterine infections, 95.0% for adnexitis, 87.0% intrapelvic infections, and 100% for external genital organ infections, making a total of 92.5%. The rate of occurrence of side effects or abnormal laboratory findings was similar to or slightly less than that seen with other beta-lactam antibiotics.

Adult↗
PubMed1982

Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis: 3. Frequent occurrence of genetic variants in some abundant polypeptides of PHA-stimulated peripheral blood lymphocytes.

The 100 or so most intensely Coomassie blue-stained polypeptides from PHA-stimulated peripheral blood lymphocytes were analyzed by two-dimensional electrophoresis in combination with family and population studies. Besides polymorphic lymphocyte cytosol 64k polypeptide reported previously, genetic variants were frequently observed in three polypeptides with molecular weights of 100,000, 49,000, and 40,000. All of them occur in the cytosol. These variant polypeptides are charge variants, because they are separated in the isoelectric focusing dimension. It is indicated by family and population studies and cell distribution analysis that the polypeptide with a molecular weight of 100,000 shows a genetic polymorphism determined by two alleles at a new autosomal locus, as described in the following paper. Family and population studies also suggest that a genetic polymorphism defined by alleles at an autosomal locus is present in each of the polypeptides with molecular weights of 49,000 and 40,000. In contrast to the previous reports of the extremely restricted genetic variability of the 100 or so most abundant fibroblast polypeptides, the present data indicate that common genetic variants are present at least in four of the 100 or so most intensely Coomassie blue-stained lymphocyte polypeptides. The result also shows that careful side-by-side comparison of two-dimensional electrophoresis patterns among both parents and their children is an effective method to detect genetic variant polypeptides.

Blood Protein Electrophoresis↗
PubMed1982

Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis: 4. Genetic polymorphism of cytosol 100k polypeptide.

We describe a genetic polymorphism of a human cellular polypeptide with mol. wt. 100,000, detected in peripheral blood lymphocytes by high resolution two-dimensional electrophoresis. Three different electrophoretic types (1-1, 2-1, and 2-2) of the polypeptide have been identified. Family and population studies indicate that the three phenotypes of the polypeptide are determined by two common alleles at a single autosomal locus. The polypeptide occurs in the cytosol and is one of the abundant polypeptides of B-lymphoblastoid cells, T-lymphoblastoid cells, fibroblasts, and HeLa cells. The data indicate that the cytosol polypeptide with mol. wt. 100,000 shows a genetic polymorphism determined by a new autosomal locus. It is proposed that the polypeptide and its locus be temporarily designated cytosol 100k polypeptide (C100k polypeptide) and C100P, respectively. In a Japanese population, the gene frequencies of C100P1 and C100P2 were 0.907 and 0.093, respectively.

Alleles↗
PubMed1982

Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis: 2. Genetic polymorphism of lymphocyte cytosol 64K polypeptide.

We describe a genetic polymorphism of human lymphocyte cytosol major polypeptide with mol. wt. 64,000, detected in peripheral blood lymphocytes by high resolution two-dimensional electrophoresis. Three different electrophoretic types (1-1, 2-1, 2-2) of the polypeptide have been identified. Family and population studies indicate that the three phenotypes of the polypeptide are determined by two common alleles at a single autosomal locus. The polypeptide occurs in the cytosol and is predominant in peripheral blood lymphocytes, B-lymphoblastoid cells, T-lymphoblastoid cells, lymph node, and spleen. The polypeptide has not been detected HeLa cells, fibroblasts, erythrocytes, serum, and cerebrum. Traces of the polypeptide exist in liver, kidney, and skeletal muscle. It is proposed that the polypeptide and its locus be temporarily designated lymphocyte cytosol 64K polypeptide (LC64K polypeptide) and LC64P, respectively. In a Japanese population, the gene frequencies of LC64P1 and LC64P2 were 0.936 and 0.064, respectively. The data suggest that LC64P is a new locus, product of which shows genetic polymorphism and is associated with the function and/or the structure of lymphocytes.

Alleles↗
PubMed1982

Genetics of receptors for bioactive polypeptides: expression of the human EGF receptor gene and internalization and processing of the receptor-bound EGF in human-mouse cell hybrids.

We previously postulated that the structural gene for epidermal growth factor (EGF) receptor is located on human chromosome 7 (1,2). In this study, EGF receptor and certain postreceptor functions were further analyzed in a unique cell hybrid line, C2B5, that retains only one human chromosome of an X;7 translocation besides a nearly complete mouse parental genome. Kinetics and Scatchard analysis of [125I]EGF binding to the C2B5 hybrid cells indicated that they carry a single class of EGF receptors with a dissociation constant of 4 x 10(-10) M. The receptors expressed in the hybrids are proven to be immunologically of human nature. The human EGF receptors now embedded in essentially mouse plasma membrane are subject to "down regulation" mediated by the ligand EGF. Analysis of the cell-bound EGF indicated that internalization and processing take place in the human-mouse cell hybrids. The degradation of EGF appears to be through a lysosomal pathway since it was substantially delayed or inhibited by lysosomotropic agents.

Animals↗
PubMed1982

Postoperative prophylactic use of progesterone in renal cell carcinoma.

Medroxyprogesterone acetate has been used prophylactically in 35 patients with stages I and IIIA renal cell carcinoma who had undergone radical nephrectomy. Metastasis was noted in 26 per cent (9 of 35) of the patients who had received the drug and in 44 per cent (20 of 45) of those who had not (controls). The incidence of metastasis in patients who had undergone nephrectomy more than 3 years previously was 10 per cent (3 of 31) for patients who received medroxyprogesterone acetate and 35 per cent (15 of 43) for the control group, the difference being statistically significant. Prognosis tended to be better in patients who had received medroxyprogesterone acetate than in the controls, and relative 3 and 5-year survival rates being 99.3 and 87.2 per cent, respectively, in the former group, and 86.8 and 75.0 per cent, respectively, in the latter group. There was no significant difference between these 2 groups. However, it may be concluded that prophylactic medroxyprogesterone acetate seems to be beneficial in the prevention of postoperative metastasis of renal cell carcinoma.

Adenocarcinoma↗