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Biomedical subjects

I Kiss

Publications and source records attributed to I Kiss.

At least 163 records · Page 9Linked to original sources
PubMed1988

Computerized automatic non-invasive blood pressure monitoring system.

A non-invasive, fully automatic blood pressure monitoring system was developed to make the diagnosis of hypertension more accurate and to help individualizing antihypertensive therapy. The system consists of two subunits: the automatic microprocessor-controlled blood pressure monitor (Nippon Colin Co. BP 203) and a microcomputer system (Commodore 64). The blood pressure monitor is fitted to the computer by our own interface and data transfer programme. Data-analysing, graphic displaying and plotting programmes were also developed by us. Circadian average blood pressure, its variability, the rhythm variabilities of chronobiological cosinor analysis: mesor, amplitude, acrophase and linear trend, have been calculated by means of these programmes. Results of blood pressure monitoring are demonstrated in a patient with essential hypertension and in a patient with renoparenchymal hypertension. The perspectives of this method in biomedical research and in clinical pharmacology are illustrated and discussed.

Antihypertensive Agents↗
PubMed1988

A comparative study of the acute antihypertensive effect of Adalat capsules and Corinfar dragées, two different nifedipine preparations.

The calcium entry blocker nifedipine produces systemic vasodilation and decreases the elevated vascular resistance in hypertensive patients. The authors investigated the acute antihypertensive effect of sublingually administered nifedipine in two different formulas: Adalat capsules and Corinfar dragées. There was no significant difference between both these formulas regarding their effects on blood pressure and heart rate, and their side effects were also similar. The authors conclude that the acute antihypertensive effect of nifedipine, either in capsules or in dragées, is equipotent in essential hypertension.

Administration, Oral↗
PubMed1987

Structure of the chicken link protein gene: exons correlate with the protein domains.

The structure of the chicken link protein gene has been determined from a series of genomic clones that cover the entire coding region as well as the complete 3'-untranslated region and a small portion of the 5'-untranslated region. The gene is greater than 80 kilobase pairs long and is present in a single copy in the chicken genome. The link protein gene contains at least five exons with four encoding the entire protein. The domain of link protein that has homologies with immunoglobulin-like proteins and the tandemly repeated hyaluronic acid binding domains are each encoded by separate exons. The exon-intron structure indicates that the link protein gene may have arisen by exon duplication and exon shuffling.

Amino Acid Sequence↗
PubMed1987

Structural features of cartilage matrix protein deduced from cDNA.

cDNAs encoding the Mr 54,000 chicken cartilage matrix protein (CMP) were selected from a cartilage cDNA expression library by immunological means. Antibodies elicited against insert-encoded protein purified from one of the clones reacted specifically with chicken CMP in immunoblots of total cartilage extract, providing positive identification of the cDNA clones isolated. The cDNAs detect a 3.4-kilobase transcript that was present in sternal cartilage and in cartilaginous but not in precartilaginous embryonic limb tissues. The cDNAs code for 416 amino acids of the chicken CMP, including its COOH terminus. There are two striking features in the deduced CMP amino acid sequence: first, it contains a region with significant homologies to repeat sequences in the precursor for epidermal growth factor; and second, it is made up of two large homologous repeat sequences. These results provide the first detailed structural information on the CMP and establish it as a developmentally regulated marker of cartilage differentiation.

Amino Acid Sequence↗
PubMed1987

The kinetics of repair in mouse lung after fractionated irradiation.

The kinetics of repair of sublethal damage in mouse lung was studied after fractionated doses of 137Cs gamma-rays. A wide range of doses per fraction (1.7-12 Gy) was given with interfraction intervals ranging from 0.5 to 24 h. The data were analysed by a direct method of analysis using the incomplete repair model. The half-time of repair (T1/2) was 0.76 h for the pneumonitis phase of damage (up to 8 months) and 0.65 h for the later phase of damage up to 12 months. The rate of repair was dependent on fraction size for both phases of lung damage and was faster after large dose fractions than after small fractions. The T1/2 was 0.6 h (95 per cent c.1. 0.53, 0.69) for doses per fraction greater than 5 Gy and 0.83 h (95 per cent c.1 0.76, 0.92) for doses per fraction of 2 Gy. Repair was nearly complete by 6 h, at least for the pneumonitis phase of damage. To the extent that extrapolation of these data to humans may be valid, these results imply that treatments with multiple fractions per day that involve the lung will not be limited by the necessity for interfraction intervals much longer than 6 h.

Animals↗
PubMed1987

Morphological studies on the articular cartilage of old rats.

Age-dependent morphological alterations of the tibial articular cartilage were studied in 26-month-old Wistar rats of both sexes. Young and adult rats (6 and 10 months of age, respectively) served as a basis for comparison. Light microscopic histology, polarization optical analysis (toluidine-blue staining with or without the addition of 0.2, 0.4 or 0.8 M MgCl2, as well as after phenol reaction) as well as transmission electron microscopy were applied. The main observations are: (1) According to the histological findings, the proportion of the zones changes with age; the subchondral bone layer becomes wider and separated to a certain extent, while the fibres become demasked. (2) Polarization microscopy revealed a disorientation of both the proteoglycane (PG) and collagen molecules; considering the available biochemical data, this phenomenon involves both quantitative and structural changes, as well as differences in the localization of the PG and collagen molecules. (3) Electron microscopy also supports the structural alterations of the collagen (thin and thick, as well as non-striated, fragmented fibres). Cellular alterations are also observed parallel with the changes of the matrix (cell organelles occur less frequently, signs of degeneration and disintegration, rupture of the plasma membrane, as well as accumulation of lipid-bodies can be seen. On the basis of the findings, one can consider the articular cartilage in the old rats as a tissue which is still in equilibrium, however, aged chondrocytes with decreased function become predominant in it. This state can be modified into a pathological one by various mechanical, endocrinological, iatrogenic, post-traumatic, etc., influences through the alterations of the cell-matrix interactions.

Aging↗
PubMed1986

Protection of mouse jejunal crypt cells by WR-2721 after small doses of radiation.

The ability of WR-2721 to protect jejunal crypt cells after single doses and multifractionated doses of radiation was studied. Effective dose survival curves for jejunal crypt cells were constructed over the dose range of 230 to 1600 cGy. WR-2721 was given 30 minutes before each fraction, in a regimen consisting of 200 mg/kg before the first radiation fraction, followed at 3 hr intervals by 100 mg/kg for a total of 12 drug doses for the largest number of fractions. Fractionation protocols were designed with common dose fractions in regimens with different fraction numbers, allowing a test of the hypothesis of equal effect per fraction and an estimate of the initial number of clonogens per crypt in both the drug treated and non-drug treated mice. The hypothesis of equal effect per fraction could not be rejected in either the drug or non-drug treated mice. An average number of 137 clonogens per crypt was estimated for the non-drug treated mice and 81 clonogens per crypt in the drug treated mice; the difference between these two values was not significant. The protection factor decreased with decreasing dose ranging from a high of 1.47 (95% C.L. = 1.44 to 1.50) after a single dose of 2000 cGy to a low of 1.21 (95% C.L. = 1.08 to 1.37) after 200 cGy. Analysis of the data using either the linear quadratic (LQ) or two-component (TC) model of cell survival showed that WR-2721 was not dose-modifying over the dose range tested. Analysis using the LQ model showed that both beta and alpha were modified by WR-2721, by 50% and 20% respectively. These data indicate that protection by WR-2721 can be expected to decrease with dose although there is some protection after clinically relevant doses.

Amifostine↗
PubMed1986

Complete amino acid sequence of chicken cartilage link protein deduced from cDNA clones.

cDNA clones coding for chicken cartilage link protein were isolated and sequenced. The DNA sequence for the entire core polypeptide of the mature link protein and the predicted signal peptide consists of 1065 nucleotides. The deduced primary translation product (355 amino acids) has a molecular mass of 40.7 kDa; the calculated molecular mass of the mature link protein core polypeptide (340 amino acids) is 39.06 kDa. The DNA sequence contains two tandemly arranged repeat sequences that may code for repeated functional domains of link protein involved in binding to hyaluronic acid. The mRNAs for chicken link protein are 6.0, 5.8, and 3.0 kilobase pairs, and the difference between the sizes of the RNA species lies in the 3' untranslated region.

Amino Acid Sequence↗
PubMed1985

Investigation on the substrate specificity of human plasmin using tripeptidyl-p-nitroanilide substrates.

The hydrolysis of 35 tripeptidyl-p-nitroanilides was studied with human plasmin and the kinetic parameters were determined. The individual contribution of the various side chains to the kinetic parameters was calculated by regression analysis. Considering Km, substrates having Z-D-Ile-Phe-Lys as well as H-D-Ile-Phe-Lys sequences were found to be the best, while Bz-Ile-Leu-Lys and pGlu-Leu-Lys sequences are the best for kcat. The Km values of substrates protected at N-terminus are lower, their kcat values are higher than those of the unprotected ones with the same sequence.

Amino Acid Sequence↗
PubMed1985

Primary structure of the telopeptide and a portion of the helical domain of chicken type II procollagen as determined by DNA sequence analysis.

A comparison of the nucleotide sequences of three new cDNA clones for chicken type II procollagen with the sequences of the other three types of chicken fibrillar procollagens reveals that the most conserved regions correlate with the positions of hydroxyproline, hydroxylysine, cysteine and lysine residues. On the basis of replacement-site-divergence calculations it is concluded that alpha 1(II) and alpha 1(I) procollagens diverged later than alpha 1(I) and alpha 2(I) procollagens.

Amino Acid Sequence↗
PubMed1985

Effect of new organophosphates on the membrane of identified central neurons of Helix pomatia L. (Mollusca, Gastropoda).

Effects of two newly synthesized organophosphates were studied on identified neurons of Helix pomatia by microelectrophysiological methods. The single intracellular spikes were processed by computer using a "phase-plane trajectory" method. Dimethoate was also involved as reference substance. The mechanism of action of the substance NE-79297 was found to be similar to that of dimethoate resulting in prolongation of action potentials due to a delayed rectification of the outward current. Phosmethylan (NE-79168), a much more selective compound, altered the membrane parameters in a different way: it affected the slow--mainly calcium-mediated--inward current.

Action Potentials↗
PubMed1985

Late functional and biochemical changes in mouse lung after irradiation: differential effects of WR-2721.

The radioprotective effect of WR-2721 on late damage after whole thorax irradiation has been studied after split doses of radiation using the standard death and breathing rate assays at monthly intervals between 3 and 15 months after irradiation, as well as two biochemical measurements of injury at 15 months, hydroxyproline (HP), an indicator of tissue fibrosis, and DNA content, an indicator of tissue cellularity. A comparison of HP/lung and breaths per minute (BPM) in each dose group in the WR-2721 and non-WR-2721-treated mice 15 months after irradiation showed that the relationship between these two assays of late lung injury was not the same. There were large dose-related increases in breathing rate corresponding to relatively small changes in HP in the lungs of mice given radiation alone. In contrast, the mice given WR-2721 before irradiation showed large dose-related increases in HP/lung, but BPM remained relatively constant independent of dose. These data suggest then that changes in breathing rate and deaths later than 9 months after whole lung irradiation may not be due to collagen accumulation in the lung. WR-2721 did protect better against late lung functional changes (protection factors (PF) = 1.6) and late deaths (PF = 1.51) than against earlier changes in these same assays (PF = 1.4 and 1.28, respectively). Although the earlier-appearing injury after whole thoracic irradiation is most likely related to lung damage with deaths and increases in breathing rate resulting from pneumonitis, the cause of the late-appearing functional injury in the lung after radiation is not clear. Thus protection of late lung damage measured from either lethality or breathing rate is not related to the prevention of lung fibrosis.

Amifostine↗