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Biomedical subjects

I Hara

Publications and source records attributed to I Hara.

At least 217 records · Page 12Linked to original sources
PubMed1983

Simple method for analysis of apolipoproteins in high density lipoproteins by high performance liquid chromatography.

A simple and rapid method has been developed for the separation of apolipoproteins in high density lipoprotein (HDL) fractions by high performance liquid chromatography (HPLC) with gel permeation columns (G3000SW TSK GEL). The HPLC pattern monitored by A280 for a mixed solution of the HDL fraction (10 microliter) and an eluent buffer (200 microliter, 0.1 M sodium phosphate buffer containing 0.1% SDS) incubated at 60 degrees C for 5 min showed two completely separated peaks which corresponded to the major components of human HDL, apolipoprotein A-I and A-II. Moreover, quantitation of apolipoprotein A-I by our method was found to correlate well with that by a single radial immunodiffusion (SRID) assay.

Apolipoproteins↗
PubMed1983

An adult case with rhabdomyosarcoma of the liver.

An autopsy case of primary rhabdomyosarcoma of the liver in a 70-year-old man is described. The case could not be clinically differentiated from hepatocellular carcinoma. The tumor showed typical features of rhabdomyosarcoma; phosphotungstic acid hematoxylin (PTAH)-positive myofibrils and cross-striations were observed in the cytoplasm of highly plemorphic tumor cells.

Aged↗
PubMed1983

Serum lipoprotein measurement--liquid chromatography and sequential floatation (ultracentrifugation) compared.

An HPLC method [J. Biochem. (Tokyo) 91:1381, 1982] was used for evaluating serum lipoproteins, with on-line monitoring of either cholesterol or phospholipids. Five well-distinguished lipoprotein fractions were observed, based on their particle sizes. Serum of 15 normal persons, 12 subjects with various types of hyperlipidemia, 20 patients with various liver diseases, and two cases of familial LCAT deficiency were examined and the results compared with those by a sequential ultracentrifugal floatation technique. In the normal group, the amounts of fractions 2, 3, and 4 by the HPLC method correlated well with concentrations of the LDL, HDL2, and HDL3 fractions as measured by the ultracentrifugal method, respectively. In the hyperlipidemic group, similar good correlations were observed between fractions 1, 2, 3, 4 and chylomicrons + VLDL, LDL, HDL2, and HDL3 fractions, respectively. For those with liver diseases or LCAT deficiency, the corresponding fractions correlated less well, and characteristically the elution profile of lipoproteins in these groups showed heterogeneity of particle size within each lipoprotein density class, especially in LDL and HDL2.

Cholesterol↗
PubMed1982

High-performance liquid chromatography of human serum lipoproteins. Selective detection of choline-containing phospholipids by enzymatic reaction.

A convenient method for the quantitation of choline-containing phospholipids in each lipoprotein fraction has been developed by combining separation by high-performance liquid chromatography with gel permeation columns and selective detection by enzymatic reaction in the post-column effluent. The elution patterns monitored by choline-containing phospholipids were compared with those monitored by cholesterol. The elution patterns of choline-containing phospholipids were found to give much more information about the distribution of lipoproteins according to their particle-size differentiation than analyses done by cholesterol. This choline-containing phospholipid monitoring method not only resolves lipoprotein peaks of the major classes (chylomicron + VLDL, LDL, HDL2 and HDL3) quantitatively, but also detects the presence of abnormal lipoproteins containing a large amount of choline-containing phospholipids. We could detect these abnormal lipoproteins using a small amount of whole serum (10-20 microliter) from patients with various liver diseases. Our examination of HDL subclasses using this technique showed that the HDL fraction was composed of several subfractions due to their particle-size differentiation.

Cholesterol↗
PubMed1982

High-performance liquid chromatography of human serum lipoproteins: selective detection of triglycerides by enzymatic reaction.

A simple and convenient method for detection and quantitation of triglycerides in each lipoprotein fraction (chylomicron, VLDL, LDL and HDL) has been developed by high-performance liquid chromatography followed by enzymatic reaction using a high-speed reaction type chromatograph. Triglycerides in serum lipoproteins eluted from the gel permeation column (TSK GEL) could be sensitively and selectively detected by the absorbance at 550 nm using a commercial reagent kit. The distribution of triglycerides in each lipoprotein fraction could be examined with a small amount of serum (10-50 microliters) in less than 50 min by this method. Moreover, free glycerol could be detected as a sharp peak at the elution volume of total permeation of the column. This technique was found to be suitable for the study of triglyceride-rich lipoproteins such as chylomicron and VLDL.

Chromatography, Gel↗
PubMed1982

Murine serum glycoprotein gp70 behaves as an acute phase reactant.

A single intraperitoneal injection of bacterial lipopolysaccharide (LPS) or its lipid A component induced high levels of glycoprotein, gp70, in sera of several strains of mice within 24 h. This serum gp70 response induced by LPS was independent of the activation of B cells and the presence of T cells. However, serological and immunohistochemical studies demonstrated the production of gp70 by hepatic parenchymal cells and its subsequent release into the circulating blood. The expression of gp70 in the serum was enhanced not only by LPS but also other inducers of acute phase reactants (APR) such as turpentine oil or polyriboinosinic-polyribocytidylic acid. Further, the serum gp70 response was kinetically identical to those of APR. These results strongly suggest that (a) the liver may be the major source for serum gp70, (b) serum gp70 behaves like an APR, (c) its expression may be controlled by a mechanism similar to that for other APR, and (d) this glycoprotein apparently behaves as a normal host constituent and not a product of a viral genome.

Animals↗
PubMed1982

Multiple primary cancers following exposure to benzidine and beta-naphthylamine.

The incidence of second primary cancers has been ascertained in 3,322 employees who had worked from 1950 to 1978 in industries in Japan that manufactured benzidine and beta-naphthylamine. Of the 244 workers who had suffered from and died of cancer of the genitourinary organs (first primary cancer), 11 (all male) had subsequent primary, histologically confirmed cancers of the liver, gallbladder, bile duct, large intestine, and lung. A control group of 177 male bladder cancer patients, assembled from the Osaka Cancer Registry during 1965-1975, showed eight cases of a second primary, five being stomach cancer. No stomach cancer was observed in the study cohort. A statistically significant excess risk for liver, gall bladder, and bile duct cancer (p less than 0.05) was found The number of observed deaths from respiratory cancer was greater than expected, though not statistically significant. These findings appear to confirm previous clinical data suggesting an increased occurrence of multiple primary cancers in workers exposed to benzidine/beta-naphthylamine, pointing to a pluripotential action of these carcinogens.

2-Naphthylamine↗
PubMed1982

Quantitation method for choline-containing phospholipids in human serum lipoproteins by high performance liquid chromatography.

A determination method for choline-containing phospholipids in serum lipoproteins was established by application of the HPLC method [Hara et al. (1980) J. Biochem. 87, 1863-1865; (1981) 89, 879-887] developed for cholesterol quantitation. The concentration of choline-containing phospholipids in the fraction separated by HPLC using gel permeation columns was determined through colorimetric detection using a commercial enzymatic kit. The optimum conditions for enzymatic reaction in the flow diagram were examined. The concentration calculated from the A500 peak area was found to reflect very precisely the concentration of choline-containing phospholipids in all lipoprotein fractions, and the quantitation of each lipoprotein fraction can be performed with only 10-20 microliters of whole serum. Monitoring the elution patterns by detecting choline-containing phospholipids can give much more information about lipoprotein distributions according to particle size than analyses done by detecting cholesterol.

Chemical Phenomena↗
PubMed1982

Heterogeneity of human serum high density lipoproteins on high performance liquid chromatography.

A combination method consisting of separation by HPLC using gel permeation columns and selective detection of cholesterol or choline-containing phospholipids was applied to the identification and quantitation of subclasses of high density lipoproteins in human serum. The frequency distribution of peaks in the elution patterns on detection of choline-containing phospholipids in a group (n = 71) of normal males and females showed five maxima. Their particle sizes were determined from the relation between Stokes' diameter and elution volume to be as follows: 122.0 +/- 2.8 A, 110.1 +/- 2.1 A, 97.5 +/- 1.8 A, 86.7 +/- 1.3 A, and 76.3 +/- 16 A. The two larger fractions were found to correspond to HDL2 subclasses, i.e., HDL2b and HDL2a. The other three peaks were found to reflect the subclasses of HDL3 and choline-containing phospholipids in the very high density lipoprotein fraction which sedimented at the bottom on ultracentrifugation at the density of 1.21. Moreover, the existence of these five subclasses in the HDL fraction was confirmed by rechromatography using this combination HPLC method. Our results for the particle sizes of HDL subclasses were confirmed by electron microscopy.

Chemical Phenomena↗
PubMed1982

[The mechanism of the development of contact sensitivity and the methods of assessing allergenicity of contact sensitizers].

Recent reports on the mechanism of the development of contact sensitivity and on the method of assessing allergenicity both in vivo and in vitro are reviewed. Various cell types and two processes, afferent and efferent limbs, involved in the contact sensitivity are discussed. Conditions for applying contact sensitizers which induce specific unresponsiveness, desensitization and antigenic competition are also discussed. Diagnostic methods for identifying contact sensitizers in vivo, patch testing, and in vitro, migration inhibition test and blastoid transformation are reported. Moreover, methods of assessing the allergenicity of contact sensitizers are reported centering on the guinea pig maximization testing.

Animals↗
PubMed1981

Low-calorie diet selectively reduces expression of retroviral envelope glycoprotein gp70 in sera of NZB x NZW F1 hybrid mice.

The effect of dietary restriction on the expression of retroviral envelope glycoprotein, gp70, and the formation of gp70-anti gp70 immune complexes was investigated in lupus-prone NZB x NZW F1 hybrid mice. Restricting total calorie intake from the usual 20 to only 10 calories per day after weaning markedly reduced serum levels of both free and antibody-complexed gp70, prevented renal disease, and increased the life spans of these mice. The reduction in serum gp70 was evident after only 2 wk of feeding these animals the low-calorie diet, and the concentration remained virtually unchanged throughout the course of 10 mon experimentation. However, serum concentrations of the major structural protein, p30, of endogenous retroviruses were not altered by restricting calories. Amounts of the serum glycoprotein, haptoglobin, decreased parallel to those of gp70 but amounts of albumin did not. These results suggest that the expression of gp70 in serum is controlled independently of the production of complete viral particles, and regulated by a mechanism similar to that for other serum glycoproteins, such as haptoglobin.

Animals↗